Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach.

abstract：:Sequence-specific gene silencing by short hairpin (sh) RNAs has recently emerged as an indispensable tool for understanding gene function and a promising avenue for drug discovery. However, a wider biomedical use of this approach is hindered by the lack of straightforward methods for achieving uniform expression of sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khandelia P,Yap K,Makeyev EV

更新日期：2011-08-02 00:00:00

abstract：:Metabolomic markers associated with incident central adiposity gain were investigated in young adults. In a 9-mo prospective study of university freshmen (n = 264). Blood samples and anthropometry measurements were collected in the first 3 d on campus and at the end of the year. Plasma from individuals was pooled by p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hootman KC,Trezzi JP,Kraemer L,Burwell LS,Dong X,Guertin KA,Jaeger C,Stover PJ,Hiller K,Cassano PA

更新日期：2017-05-23 00:00:00

abstract：:Psoralens linked to triplex-forming oligonucleotides (psoTFOs) have been used in conjunction with laser-induced two-photon excitation (TPE) to damage a specific DNA target sequence. To demonstrate that TPE can initiate photochemistry resulting in psoralen-DNA photoadducts, target DNA sequences were incubated with psoT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oh DH,King BA,Boxer SG,Hanawalt PC

更新日期：2001-09-25 00:00:00

abstract：:The S region of the murine major histocompatibility complex contains two structurally related genes (21-OHase A and 21-OHase B) that encode 21-hydroxylase (21-OHase), an enzyme essential for the synthesis of adrenal steroids. Expression of these two genes has been analyzed by using oligonucleotide probes specific for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker KL,Chaplin DD,Wong M,Seidman JG,Smith JA,Schimmer BP

更新日期：1985-12-01 00:00:00

abstract：:Bone mineralization is an essential step during the embryonic development of vertebrates, and bone serves vital functions in human physiology. To systematically identify unique gene functions essential for osteogenesis, we performed a forward genetic screen in zebrafish and isolated a mutant, no bone (nob), that does ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huitema LF,Apschner A,Logister I,Spoorendonk KM,Bussmann J,Hammond CL,Schulte-Merker S

更新日期：2012-12-26 00:00:00

abstract：:Homozygous uniparental mouse embryos, produced by microsurgical removal of the male pronucleus from fertilized eggs and diploidization of the female pronucleus with cytochalasin, were surrounded with blastomeres from normal embryos to produce chimeric embryos. A few of these chimeras developed into viable adults, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderegg C,Markert CL

更新日期：1986-09-01 00:00:00

abstract：:Structure and function of p185neu receptor tyrosine kinase were found to be regulated in a cell cycle-dependent manner. In M phase, p185neu is hyperphosphorylated at serine and/or threonine residues. The phosphotyrosine [Tyr(P)] content of p185neu is at its highest level in G0/G1 phase, decreases through S and G2 phas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiyokawa N,Yan DH,Brown ME,Hung MC

更新日期：1995-02-14 00:00:00

abstract：:In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Aalten DM,Synstad B,Brurberg MB,Hough E,Riise BW,Eijsink VG,Wierenga RK

更新日期：2000-05-23 00:00:00

abstract：:The linear ubiquitin assembly complex (LUBAC) comprises 3 components: HOIP, HOIL-1, and Sharpin, of which HOIP and HOIL-1 are both members of the RBR subfamily of E3 ubiquitin ligases. HOIP catalyses the formation of Met1-linked ubiquitin oligomers (also called linear ubiquitin), but the function of the E3 ligase acti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelsall IR,Zhang J,Knebel A,Arthur JSC,Cohen P

更新日期：2019-07-02 00:00:00

abstract：:Break-induced replication (BIR) refers to recombination-dependent DNA synthesis initiated from one end of a DNA double-strand break and can extend for more than 100 kb. BIR initiates by Rad51-catalyzed strand invasion, but the mechanism for DNA synthesis is not known. Here, we used BrdU incorporation to track DNA synt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donnianni RA,Symington LS

更新日期：2013-08-13 00:00:00

abstract：:We have totally synthesized a gene that codes for rat hepatic cytochrome b5. The 5' flanking region was designed for efficient expression of this gene in Escherichia coli by incorporating an optimum ribosome binding site and spacer region. Both a soluble form, analogous to the protease-treated microsomal protein, as w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beck von Bodman S,Schuler MA,Jollie DR,Sligar SG

更新日期：1986-12-01 00:00:00

abstract：:Excitation of HbCO by a single 6-psec 530-nm pulse results in photodissociation with a first-order constant of 0.89 X 10(11) sec-1. The kinetics of photodissociation, monitored by following absorbance changes in the Soret band at 440 nm, are interpreted as corresponding to predissociation followed by a corssing into a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noe LJ,Eisert WG,Rentzepis PM

更新日期：1978-02-01 00:00:00

abstract：:Transcript accumulation and translation of psbA as well as processing of the D1 precursor protein were investigated in relation to chlorophyll availability in vivo in cyanobacterial strains lacking photosystem I (PS I). The psbA transcript level was almost independent of chlorophyll availability and was approximately ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Q,Vermaas W

更新日期：1998-05-12 00:00:00

abstract：:To determine which of seven library design algorithms best introduces new protein function without destroying it altogether, seven combinatorial libraries of green fluorescent protein variants were designed and synthesized. Each was evaluated by distributions of emission intensity and color compiled from measurements ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Treynor TP,Vizcarra CL,Nedelcu D,Mayo SL

更新日期：2007-01-02 00:00:00

abstract：:Cyanogen bromide fragments were isolated from the heavy chains of three human IgG myeloma proteins of the V(H)III subgroup, sequenced by an automated method, and localized to the variable region. Inspection of these sequences, together with corresponding stretches from both human and animal proteins (studied in other ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kehoe JM,Capra JD

更新日期：1971-09-01 00:00:00

abstract：:We studied the surface delivery pathways followed by newly synthesized plasma membrane proteins in intestinal cells. To this end, we developed an assay and characterized an epithelial cell line (SK-CO-15) derived from human colon adenocarcinoma. Polarized confluent monolayers (2000 omega.cm2), grown on polycarbonate f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Bivic A,Real FX,Rodriguez-Boulan E

更新日期：1989-12-01 00:00:00

abstract：:We reported previously that human cells after neoplastic transformation in culture had acquired an increased susceptibility to chromatid damage induced by x-irradiation during the G2 phase of the cell cycle. Evidence suggested that this results from deficient DNA repair during G2 phase. Cells derived from human tumors...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parshad R,Sanford KK,Jones GM

更新日期：1985-08-01 00:00:00

abstract：:Several gene fusions have been constructed in which coding sequences for antigenic regions of the pre-S sequences of hepatitis B virus, hepatitis B surface antigen, and the envelope protein of human immunodeficiency virus were linked to the 3' end of that for the first 144 residues of hepatitis B core antigen. The seq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stahl SJ,Murray K

更新日期：1989-08-01 00:00:00

abstract：:Fms-like tyrosine kinase 1 (Flt1)/vascular endothelial growth factor (VEGF) receptor 1, a receptor for VEGF-A and placental growth factor, is expressed in the spongiotrophoblast layer that segregates the maternal and fetal vasculature in the mouse placenta. A soluble form of Flt1 (sFlt1) produced in the mouse and huma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirashima M,Lu Y,Byers L,Rossant J

更新日期：2003-12-23 00:00:00

abstract：:Understanding the complexity of anisotropic turbulent processes over a wide range of spatiotemporal scales in engineering shear turbulence as well as climate atmosphere ocean science is a grand challenge of contemporary science with important societal impact. In such inhomogeneous turbulent dynamical systems there is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majda AJ

更新日期：2015-07-21 00:00:00

abstract：:The Late Heavy Bombardment (LHB), a hypothesized impact spike at ∼3.9 Ga, is one of the major scientific concepts to emerge from Apollo-era lunar exploration. A significant portion of the evidence for the existence of the LHB comes from histograms of (40)Ar/(39)Ar "plateau" ages (i.e., regions selected on the basis of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boehnke P,Harrison TM

更新日期：2016-09-27 00:00:00

abstract：:The B-cell antigen receptor is composed of membrane immunoglobulin sheathed by an alpha/beta heterodimer. The complex is noncovalently associated with protein kinase activity, and crosslinking of the receptor leads to capping and transmembrane signaling. Here we show that the sheath is not necessary either for this ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams GT,Peaker CJ,Patel KJ,Neuberger MS

更新日期：1994-01-18 00:00:00

abstract：:The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47-->methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skinner MM,Zhang H,Leschnitzer DH,Guan Y,Bellamy H,Sweet RM,Gray CW,Konings RN,Wang AH,Terwilliger TC

更新日期：1994-03-15 00:00:00

abstract：:The Arabidopsis thaliana disease resistance genes RPS2 and RPM1 belong to a class of plant disease resistance genes that encode proteins that contain an N-terminal tripartite nucleotide binding site (NBS) and a C-terminal tandem array of leucine-rich repeats. RPS2 and RPM1 confer resistance to strains of the bacterial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leister RT,Ausubel FM,Katagiri F

更新日期：1996-12-24 00:00:00

abstract：:In the nitrogen-fixing symbiosis between legume hosts and rhizobia, the bacteria are engulfed by a plant cell membrane to become intracellular organelles. In the model legume Medicago truncatula, internalization and differentiation of Sinorhizobium (also known as Ensifer) meliloti is a prerequisite for nitrogen fixati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim M,Chen Y,Xi J,Waters C,Chen R,Wang D

更新日期：2015-12-08 00:00:00

abstract：:Game theory describes strategic interactions where success of players' actions depends on those of coplayers. In humans, substantial progress has been made at the neural level in characterizing the dopaminergic and frontostriatal mechanisms mediating such behavior. Here we combined computational modeling of strategic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Set E,Saez I,Zhu L,Houser DE,Myung N,Zhong S,Ebstein RP,Chew SH,Hsu M

更新日期：2014-07-01 00:00:00

abstract：:The human placental alkaline phosphatase gene has been cloned and reintroduced into mammalian cells. When a plasmid carrying the gene under control of the simian virus 40 early promoter (pSV2Apap) is transfected into a variety of different cell types, placental alkaline phosphatase activity can readily be detected by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henthorn P,Zervos P,Raducha M,Harris H,Kadesch T

更新日期：1988-09-01 00:00:00

abstract：:The [PSI+] prion is a self-propagating amyloid of the translation termination factor, Sup35p, of Saccharomyces cerevisiae. The N-terminal 253 residues (NM) of this 685-residue protein normally function in regulating mRNA turnover but spontaneously form infectious amyloid in vitro. We converted the three Ile residues i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorkovskiy A,Thurber KR,Tycko R,Wickner RB

更新日期：2014-10-28 00:00:00

abstract：:Concepts organize the relationship among individual stimuli or events by highlighting shared features. Often, new goals require updating conceptual knowledge to reflect relationships based on different goal-relevant features. Here, our aim is to determine how hippocampal (HPC) object representations are organized and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mack ML,Love BC,Preston AR

更新日期：2016-11-15 00:00:00

abstract：:The human carcinogen vinyl chloride is metabolized in the liver to reactive intermediates which form N2,3-ethenoguanine in DNA. N2,3-Ethenoguanine is known to cause G----A transitions during DNA replication in Escherichia coli, and its formation may be a carcinogenic event in higher organisms. To investigate the repai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matijasevic Z,Sekiguchi M,Ludlum DB

更新日期：1992-10-01 00:00:00