Immunogenicity of peptide fusions to hepatitis B virus core antigen.

abstract：:We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dirks RM,Pierce NA

更新日期：2004-10-26 00:00:00

abstract：:Tolerance and resistance provide hosts with two distinct defense strategies against parasitism. In resistance the hosts "fight" the parasite directly, whereas in tolerance the hosts fight the disease by ameliorating the damage that infection causes. There is increasing recognition that the two mechanisms may exhibit v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Best A,White A,Boots M

更新日期：2008-12-30 00:00:00

abstract：:Remembering is a complex process that involves recalling specific details, such as who you were with when you celebrated your last birthday, as well as contextual information, such as the place where you celebrated. It is well established that the act of remembering enhances long-term retention of the retrieved inform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jonker TR,Dimsdale-Zucker H,Ritchey M,Clarke A,Ranganath C

更新日期：2018-10-23 00:00:00

abstract：:GLQ223 is a highly purified, formulated preparation of trichosanthin, a 26-kDa plant-derived ribosome-inactivating protein with potent inhibitory activity against human immunodeficiency virus (HIV) in vitro. The compound produced concentration-dependent inhibition of HIV replication in acutely infected cultures of T-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McGrath MS,Hwang KM,Caldwell SE,Gaston I,Luk KC,Wu P,Ng VL,Crowe S,Daniels J,Marsh J

更新日期：1989-04-01 00:00:00

abstract：:The selective alteration of the cellular genome by laser microbeam irradiation has been extensively applied in cell biology. We report here the use of the third harmonic (355 nm) of an yttrium-aluminum garnet laser to facilitate the direct transfer of the neo gene into cultured human HT1080-6TG cells. The resultant tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao W,Wilkinson J,Stanbridge EJ,Berns MW

更新日期：1987-06-01 00:00:00

abstract：:Using currently available gene fusion techniques, we have constructed plasmids that direct the overproduction of the carboxyl-terminal two-thirds of DNA polymerase I, corresponding to the proteolytically derived "Klenow fragment." We have obtained overproduction amounting to several percent of the cellular protein usi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joyce CM,Grindley ND

更新日期：1983-04-01 00:00:00

abstract：:Equity is of fundamental concern in the quest for international cooperation to stabilize greenhouse gas concentrations by the reduction of emissions. By modeling the carbon cycle, we estimate the global CO(2) emissions that would be required to stabilize the atmospheric concentration of CO(2) at levels ranging from 45...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolin B,Kheshgi HS

更新日期：2001-04-24 00:00:00

abstract：:A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as a stable plasmid or can be packaged into infective bacteriophage particles. Replication of P420, directed by the ColE1 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kahn M,Helinski DR

更新日期：1978-05-01 00:00:00

abstract：:Preparations of partially purified chloroplast DNA-dependent RNA polymerase from maize and some other plants transcribe cloned chloroplast genes preferentially and much more actively from appropriately negatively supercoiled templates than from relaxed templates. We have found that the polymerase in such fractions doe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaitlin D,Hu J,Bogorad L

更新日期：1989-02-01 00:00:00

abstract：:Hendra virus (HeV) and Nipah virus (NiV) belong to the genus Henipavirus of the family Paramyxoviridae and are unique in that they exhibit a broad species tropism and cause fatal disease in both animals and humans. They infect cells through a pH-independent membrane fusion process mediated by their fusion and attachme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonaparte MI,Dimitrov AS,Bossart KN,Crameri G,Mungall BA,Bishop KA,Choudhry V,Dimitrov DS,Wang LF,Eaton BT,Broder CC

更新日期：2005-07-26 00:00:00

abstract：:Treatment strategies for severe cases of pandemic influenza have focused on antiviral therapies. In contrast, passive immunotherapy with convalescent blood products has received limited attention. We consider the hypothesis that a passive-immunotherapy program that collects plasma from a small percentage of recovered ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu JT,Lee CK,Cowling BJ,Yuen KY

更新日期：2010-02-16 00:00:00

abstract：:To achieve accurate aminoacylation of tRNAs with their cognate amino acids, errors in aminoacylation are corrected by the "editing" mechanism in several aminoacyl-tRNA synthetases. Phenylalanyl-tRNA synthetase (PheRS) hydrolyzes, or edits, misformed tyrosyl-tRNA with its editing domain in the beta subunit. We report t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasaki HM,Sekine S,Sengoku T,Fukunaga R,Hattori M,Utsunomiya Y,Kuroishi C,Kuramitsu S,Shirouzu M,Yokoyama S

更新日期：2006-10-03 00:00:00

abstract：:Cell-mediated immunity is critical for host resistance to tuberculosis. T lymphocytes recognizing antigens presented by the major histocompatibility complex (MHC) class I and class II molecules have been found to be necessary for control of mycobacterial infection. Mice genetically deficient in the generation of MHC c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teitelbaum R,Cammer M,Maitland ML,Freitag NE,Condeelis J,Bloom BR

更新日期：1999-12-21 00:00:00

abstract：:The interaction between cells and nanostructured materials is attracting increasing interest, because of the possibility to open up novel concepts for the design of smart nanobiomaterials with active biological functionalities. In this frame we investigated the response of human neuroblastoma cell line (SH-SY5Y) to go...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brunetti V,Maiorano G,Rizzello L,Sorce B,Sabella S,Cingolani R,Pompa PP

更新日期：2010-04-06 00:00:00

abstract：:We have devised a general strategy for gene mapping based upon the direct amplification of a target sequence within a single microdissected Giemsa-banded chromosomal segment using the polymerase chain reaction. The usefulness of this approach was demonstrated by mapping a cloned human brain sodium channel (alpha subun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han JA,Lu CM,Brown GB,Rado TA

更新日期：1991-01-15 00:00:00

abstract：:Chloroplasts have evolved from a cyanobacterial endosymbiont and been retained for more than 1 billion years by coordinated chloroplast division in multiplying eukaryotic cells. Chloroplast division is performed by ring structures at the division site, encompassing both the inside and the outside of the two envelopes....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyagishima SY,Kuwayama H,Urushihara H,Nakanishi H

更新日期：2008-09-30 00:00:00

abstract：:Curcumin (Cur) is a naturally occurring anticancer drug isolated from the Curcuma longa plant. It is known to exhibit anticancer properties via inhibiting the STAT3 phosphorylation process. However, its poor water solubility and low bioavailability impede its clinical application. Herein, we used organoplatinum(II) ← ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Datta S,Misra SK,Saha ML,Lahiri N,Louie J,Pan D,Stang PJ

更新日期：2018-08-07 00:00:00

abstract：:Patients with systemic lupus erythematosus (SLE) have accelerated atherosclerosis. The underlying mechanisms are poorly understood, and investigations have been hampered by the absence of animal models that reflect the human condition of generalized atherosclerosis and lupus. We addressed this problem by transferring ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanic AK,Stein CM,Morgan AC,Fazio S,Linton MF,Wakeland EK,Olsen NJ,Major AS

更新日期：2006-05-02 00:00:00

abstract：:Rearrangements between tandem sequence homologies of various lengths are a major source of genomic change and can be deleterious to the organism. These rearrangements can result in either deletion or duplication of genetic material flanked by direct sequence repeats. Molecular genetic analysis of repetitive sequence i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bzymek M,Lovett ST

更新日期：2001-07-17 00:00:00

abstract：:Here we study the effects of many-body interactions on rate and mechanism in protein folding by using the results of molecular dynamics simulations on numerous coarse-grained Calpha-model single-domain proteins. After adding three-body interactions explicitly as a perturbation to a Gō-like Hamiltonian with native pair...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ejtehadi MR,Avall SP,Plotkin SS

更新日期：2004-10-19 00:00:00

abstract：:The SCF ubiquitin ligase complex of budding yeast triggers DNA replication by catalyzing ubiquitination of the S phase cyclin-dependent kinase inhibitor SIC1. SCF is composed of three proteins-ySKP1, CDC53 (Cullin), and the F-box protein CDC4-that are conserved from yeast to humans. As part of an effort to identify co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lyapina SA,Correll CC,Kipreos ET,Deshaies RJ

更新日期：1998-06-23 00:00:00

abstract：:Humans who have inherited the class I major histocompatibility allele HLA-A29 have a markedly increased relative risk of developing the eye disease termed birdshot chorioretinopathy. This disease affecting adults is characterized by symmetrically scattered, small, cream-colored spots in the fundus associated with reti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szpak Y,Vieville JC,Tabary T,Naud MC,Chopin M,Edelson C,Cohen JH,Dausset J,de Kozak Y,Pla M

更新日期：2001-02-27 00:00:00

abstract：:The microvillus core-filament bundle from intestinal epithelial cells is a highly ordered structure containing actin and four major associated proteins. Two of these, villin and calmodulin, bind calcium ions (Kd approximately 10(-6) M) in the physiologically important range. Because ethylene glycol bis(beta-aminoethyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glenney JR Jr,Bretscher A,Weber K

更新日期：1980-11-01 00:00:00

abstract：:Creatine kinase (CK; EC 2.7.3.2) plays an important role in energy metabolism in brain and muscle. Expression of CK isoenzymes is regulated during development and is tissue specific. To define the structures of canine CK isoenzymes and to elucidate the mechanism of regulation in their expression, CK cDNA clones from d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roman D,Billadello J,Gordon J,Grace A,Sobel B,Strauss A

更新日期：1985-12-01 00:00:00

abstract：:A remote labeling method has been developed to determine (18)O kinetic isotope effects (KIEs) in Ras-catalyzed GTP hydrolysis. Substrate mixtures consist of (13)C-depleted GTP and [(18)O,(13)C]GTP that contains (18)O at phosphoryl positions of mechanistic interest and (13)C at all carbon positions of the guanosine moi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du X,Black GE,Lecchi P,Abramson FP,Sprang SR

更新日期：2004-06-15 00:00:00

abstract：:Heat shock factors (HSFs) are the master regulators of transcription under protein-damaging conditions, acting in an environment where the overall transcription is silenced. We determined the genomewide transcriptional program that is rapidly provoked by HSF1 and HSF2 under acute stress in human cells. Our results rev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vihervaara A,Sergelius C,Vasara J,Blom MA,Elsing AN,Roos-Mattjus P,Sistonen L

更新日期：2013-09-03 00:00:00

abstract：:Energy-storing artificial-photosynthetic systems for CO2 reduction must derive the reducing equivalents from a renewable source rather than from sacrificial donors. To this end, a homogeneous, integrated chromophore/two-catalyst system is described that is thermodynamically capable of photochemically driving the energ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： La Porte NT,Moravec DB,Hopkins MD

更新日期：2014-07-08 00:00:00

abstract：:Autophagy is a cellular catabolic pathway by which long-lived proteins and damaged organelles are targeted for degradation. Activation of autophagy enhances cellular tolerance to various stresses. Recent studies indicate that a class of anticancer agents, histone deacetylase (HDAC) inhibitors, can induce autophagy. On...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gammoh N,Lam D,Puente C,Ganley I,Marks PA,Jiang X

更新日期：2012-04-24 00:00:00

abstract：:Prior studies have identified a subset of dendritic cells in human blood, as well as their stimulatory function for T-cell-mediated immune responses. However research has been limited by difficulties in isolation, since dendritic cells make up only 0.1-1% of blood mononuclear cells. We present a protocol that reliably...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freudenthal PS,Steinman RM

更新日期：1990-10-01 00:00:00

abstract：:The minimal size of the recombination site required for efficient FLP recombinase-catalyzed recombination in vitro is no more than 28 base pairs, which includes parts of two 13-base-pair inverted repeats and all of an 8-base-pair spacer. The FLP recombinase cleaves the DNA at the boundaries of the spacer, becomes cova...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senecoff JF,Bruckner RC,Cox MM

更新日期：1985-11-01 00:00:00