Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.

abstract：:We present here direct evidence for the preservation of a transcriptional initiation sequence in a eukaryotic rRNA precursor: the 5'-end group for precursor to 17S rRNA (p17S RNA) from Dictyostelium discoideum is identified as the triphosphate residue pppA-. We also show that mature 5S RNA form Dictyostelium bears a d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Batts-Young B,Lodish HF

更新日期：1978-02-01 00:00:00

abstract：:We report the isolation and functional characterization of cDNAs encoding a Drosophila kainate-selective glutamate receptor. The deduced mature 964-residue protein (DGluR-I) is 108,482 Da and exhibits significant homology to mammalian glutamate receptor subunits. Injection of DGluR-I cRNA into Xenopus oocytes generate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ultsch A,Schuster CM,Laube B,Schloss P,Schmitt B,Betz H

更新日期：1992-11-01 00:00:00

abstract：:The bacterial actin homolog, MreB, forms helical cables within the cell that are required for maintenance of a rod shape. These helical structures are thought to be involved in the spatial organization of cell wall (peptidoglycan) synthesizing complexes of penicillin-binding proteins (PBPs). Here, we examined the role...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Divakaruni AV,Loo RR,Xie Y,Loo JA,Gober JW

更新日期：2005-12-20 00:00:00

abstract：:For length and area, a central fact is that the value of the length of a curve or the area of a surface, as given by the Lebesgue theory, is at least as great as that given by the classical formula, whenever the latter has meaning. This is now found not to be valid in higher dimensions. We give an example of a continu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goffman C

更新日期：1969-05-01 00:00:00

abstract：:Psychedelic drugs have a long history of use in healing ceremonies, but despite renewed interest in their therapeutic potential, we continue to know very little about how they work in the brain. Here we used psilocybin, a classic psychedelic found in magic mushrooms, and a task-free functional MRI (fMRI) protocol desi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carhart-Harris RL,Erritzoe D,Williams T,Stone JM,Reed LJ,Colasanti A,Tyacke RJ,Leech R,Malizia AL,Murphy K,Hobden P,Evans J,Feilding A,Wise RG,Nutt DJ

更新日期：2012-02-07 00:00:00

abstract：:Single strands of DNA serve, in rare instances, as promoters for transcription; duplex DNA promoters with individual strands that also have a promoter capacityfunction have not been described. We show that the nontranscribed strand of the Saccharomyces cerevisiae U6 snRNA gene directs transcription initiation factor I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schroder O,Geiduschek EP,Kassavetis GA

更新日期：2003-02-04 00:00:00

abstract：:Analysis of previously published sets of DNA microarray gene expression data by singular value decomposition has uncovered underlying patterns or "characteristic modes" in their temporal profiles. These patterns contribute unequally to the structure of the expression profiles. Moreover, the essential features of a giv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holter NS,Mitra M,Maritan A,Cieplak M,Banavar JR,Fedoroff NV

更新日期：2000-07-18 00:00:00

abstract：:We studied the effect of gangliosides GD1a and GM1 on the lamellar-to-hexagonal II phase transition of mixtures of dioleoylphosphatidylethanolamine/dioleoylphosphatidyl choline, 3:1, and of transphosphatidylated phosphatidylethanolamine with dioleoylglycerol by high-sensitivity differential scanning calorimetry, 31P-N...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perillo MA,Scarsdale NJ,Yu RK,Maggio B

更新日期：1994-10-11 00:00:00

abstract：:GTP-tubulin forms a cap on microtubule ends during aggregation. The bulk of the microtubule is GDP-tubulin. This complicates the usual simple kinetic theory of subunit exchange at microtubule ends to such an extent that Monte Carlo calculations are needed to handle the complications, except in special cases. The Monte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Hill TL

更新日期：1983-12-01 00:00:00

abstract：:The mechanisms that regulate insulin secretion were investigated using capacitance measurements of exocytosis in single beta cells maintained in tissue culture. Exocytosis was stimulated by voltage-clamp depolarizations to activate the voltage-dependent Ca2+ channels that mediate Ca2+ influx into the beta cell. Under ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ammälä C,Eliasson L,Bokvist K,Berggren PO,Honkanen RE,Sjöholm A,Rorsman P

更新日期：1994-05-10 00:00:00

abstract：:Preadipocytes can give rise to either white adipocytes or beige adipocytes. Owing to their distinct abilities in nutrient storage and energy expenditure, strategies that specifically promote "beiging" of adipocytes hold great promise for counterbalancing obesity and metabolic diseases. Yet, factors dictating the diffe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu K,Lin L,Li Q,Xue Y,Zheng F,Wang G,Zheng C,Du L,Hu M,Huang Y,Shao C,Kong X,Melino G,Shi Y,Wang Y

更新日期：2020-02-04 00:00:00

abstract：:The adrenergic receptors (ARs) (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. We have previously assigned the genes for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang-Feng TL,Xue FY,Zhong WW,Cotecchia S,Frielle T,Caron MG,Lefkowitz RJ,Francke U

更新日期：1990-02-01 00:00:00

abstract：:Half-cystine peptic peptides representing the intrachain disulfide bonds of the light chain from an apparently homogeneous rabbit anti-p-azobenzoate antibody were isolated in good yields after reduction and alkylation with [(14)C]iodoacetate, and their sequences were determined. The sequence of the first 21 amino-term...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Appella E,Chersi A,Roholt OA,Pressman D

更新日期：1971-10-01 00:00:00

abstract：:Ocean acidification represents a key threat to coral reefs by reducing the calcification rate of framework builders. In addition, acidification is likely to affect the relationship between corals and their symbiotic dinoflagellates and the productivity of this association. However, little is known about how acidificat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anthony KR,Kline DI,Diaz-Pulido G,Dove S,Hoegh-Guldberg O

更新日期：2008-11-11 00:00:00

abstract：:Complexin activates Ca(2+)-triggered neurotransmitter release and regulates spontaneous release in the presynaptic terminal by cooperating with the neuronal soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and the Ca(2+)-sensor synaptotagmin. The N-terminal domain of complexin is importa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai Y,Choi UB,Zhang Y,Zhao M,Pfuetzner RA,Wang AL,Diao J,Brunger AT

更新日期：2016-08-09 00:00:00

abstract：:DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high-resolution NMR and x-ray studies, the origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbic A,Zimmer DP,Crothers DM

更新日期：2003-03-04 00:00:00

abstract：:One goal of regenerative medicine is to repair damaged tissue. This requires not only generating new cells of the proper phenotype, but also selecting for those that properly integrate into sites of injury. In our laboratory we are using a cell-migration-based in vivo selection system to generate antibodies that induc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han KH,Arlian BM,Macauley MS,Paulson JC,Lerner RA

更新日期：2018-01-16 00:00:00

abstract：:The formation of the past tense of verbs in English has been the focus of the debate concerning connectionist vs. symbolic accounts of language. Brain-injured patients differ with respect to whether they are more impaired in generating irregular past tenses (TAKE-TOOK) or past tenses for nonce verbs (WUG-WUGGED). Such...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joanisse MF,Seidenberg MS

更新日期：1999-06-22 00:00:00

abstract：:Chloroplast DNA of the protozoan flagellate, Euglena gracilis, exists as circular molecules, 40 mum in contour length, as shown by electron microscopy and buoyant density analyses. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning JE,Wolstenholme DR,Ryan RS,Hunter JA,Richards OC

更新日期：1971-06-01 00:00:00

abstract：:The evolution of development has been studied through the lens of gene regulation by examining either closely related species or extremely distant animals of different phyla. In nematodes, detailed cell- and stage-specific expression analyses are focused on the model Caenorhabditis elegans, in part leading to the view...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schiffer PH,Polsky AL,Cole AG,Camps JIR,Kroiher M,Silver DH,Grishkevich V,Anavy L,Koutsovoulos G,Hashimshony T,Yanai I

更新日期：2018-04-24 00:00:00

abstract：:Fifty cats with feline leukemia virus (FeLV) infection and leukemia-lymphoma complex were treated by ex vivo immunoadsorption with Staphylococcus protein A-bound filters. Most cats responded to therapy. Twelve showed tumor regression, including disappearance of tumor cells, but died later of other complications. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu WT,Engelman RW,Trang LQ,Hau K,Good RA,Day NK

更新日期：1984-06-01 00:00:00

abstract：:The type 6 secretion system (T6SS) is a nanomachine used by many Gram-negative bacteria, including Vibrio cholerae, to deliver toxic effector proteins into adjacent eukaryotic and bacterial cells. Because the activity of the T6SS is dependent on direct contact between cells, its activity is limited to bacteria growing...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toska J,Ho BT,Mekalanos JJ

更新日期：2018-07-31 00:00:00

abstract：:A variety of agricultural plant species, including corn, respond to insect herbivore damage by releasing large quantities of volatile compounds and, as a result, become highly attractive to parasitic wasps that attack the herbivores. An elicitor of plant volatiles, N-(17-hydroxylinolenoyl)-L-glutamine, named volicitin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paré PW,Alborn HT,Tumlinson JH

更新日期：1998-11-10 00:00:00

abstract：:We tested the possibility that insulin-like growth factor I (IGF-I) acts as a neuromodulator in the adult cerebellar cortex since previous observations indicated that IGF-I is located in the olivo-cerebellar system encompassing the inferior olive and Purkinje cells. We found that conjoint administration of IGF-I and g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castro-Alamancos MA,Torres-Aleman I

更新日期：1993-08-01 00:00:00

abstract：:Protection of poikilothermic animals from seasonal cold is widely regarded as being causally linked to changes in the unsaturation of membrane phospholipids, yet in animals this proposition remains formally untested. We have now achieved this by the genetic manipulation of lipid biosynthesis of Caenorhabditis elegans ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murray P,Hayward SA,Govan GG,Gracey AY,Cossins AR

更新日期：2007-03-27 00:00:00

abstract：:The Na,K-ATPase contains a binding site for cardiac glycosides, such as ouabain, digoxin, and digitoxin, which is highly conserved among species ranging from Drosophila to humans. Although advantage has been taken of this site to treat congestive heart failure with drugs such as digoxin, it is unknown whether this sit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dostanic-Larson I,Van Huysse JW,Lorenz JN,Lingrel JB

更新日期：2005-11-01 00:00:00

abstract：:Expression of the Arabidopsis TRANSPARENT TESTA 2 (TT2) MYB family transcription factor leads to massive accumulation of proanthocyanidins (PAs) in hairy roots of Medicago truncatula. Microarray analysis showed that TT2 induces genes for flavonoid/PA biosynthesis, transcription factors, and a large number of genes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pang Y,Peel GJ,Sharma SB,Tang Y,Dixon RA

更新日期：2008-09-16 00:00:00

abstract：:Protein acetylation, especially histone acetylation, is the subject of both research and clinical investigation. At least four small-molecule histone deacetylase inhibitors are currently in clinical trials for the treatment of cancer. These and other inhibitors also affect microtubule acetylation. A multidimensional, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haggarty SJ,Koeller KM,Wong JC,Grozinger CM,Schreiber SL

更新日期：2003-04-15 00:00:00

abstract：:To study the origin of and the degree of T-cell antigen receptor (TCR) diversity of Thy-1+ dendritic epidermal cells (Thy-1+ dECs) in mice, we have developed a monoclonal antibody (mAb 536) to the gamma delta TCR. mAb 536 binds to and stimulates interleukin 2 secretion from Thy-1+ dEC but not cells that express TCR co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Havran WL,Grell S,Duwe G,Kimura J,Wilson A,Kruisbeek AM,O'Brien RL,Born W,Tigelaar RE,Allison JP

更新日期：1989-06-01 00:00:00

abstract：:Despite the success statistical physics has enjoyed at predicting the properties of materials for given parameters, the inverse problem, identifying which material parameters produce given, desired properties, is only beginning to be addressed. Recently, several methods have emerged across disciplines that draw upon o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miskin MZ,Khaira G,de Pablo JJ,Jaeger HM

更新日期：2016-01-05 00:00:00