Abstract:
:Single strands of DNA serve, in rare instances, as promoters for transcription; duplex DNA promoters with individual strands that also have a promoter capacityfunction have not been described. We show that the nontranscribed strand of the Saccharomyces cerevisiae U6 snRNA gene directs transcription initiation factor IIIB-requiring and accurately initiating transcription by RNA polymerase III. The nontranscribed strand promoter is much more extended than its duplex DNA counterpart, comprising the U6 gene TATA box, a downstream T(7) tract, and an upstream-lying segment. A requirement for placement of the 3' end of the transcribed (template) strand within the confines of the transcription bubble is seen as indicating that the nontranscribed strand provides a scaffold for RNA polymerase recruitment but is deficient at a subsequent step of transcription initiation factor IIIB's direct involvement in promoter opening.
journal_name
Proc Natl Acad Sci U S Aauthors
Schroder O,Geiduschek EP,Kassavetis GAdoi
10.1073/pnas.242735699keywords:
subject
Has Abstractpub_date
2003-02-04 00:00:00pages
934-9issue
3eissn
0027-8424issn
1091-6490pii
242735699journal_volume
100pub_type
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