A rodent model for Wilms tumors: embryonal kidney neoplasms induced by N-nitroso-N'-methylurea.

abstract：:We describe the preparation and structural analysis of ordered tubular arrays of the actin-DNase I complex. These structures consist of helically stacked rings; each ring is 73 A thick, has a 240 A outer and a 120 A inner diameter, and has 7-fold rotational symmetry. The actin-DNase I complex forms tubes under conditi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fowler WE,Buhle EL,Aebi U

更新日期：1984-03-01 00:00:00

abstract：:We have developed a color complementation assay that allows rapid screening of specific genomic DNA sequences. It is based on the simultaneous amplification of two or more DNA segments with fluorescent oligonucleotide primers such that the generation of a color, or combination of colors, can be visualized and used for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chehab FF,Kan YW

更新日期：1989-12-01 00:00:00

abstract：:The major Hsp70 of the mitochondrial matrix (Ssc1 in yeast) is critically important for the translocation of proteins from the cytosol, across the mitochondrial inner membrane, and into the matrix. Tim44, a peripheral inner membrane protein with limited sequence similarity to the J domain of J-type cochaperones, tethe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'Silva PD,Schilke B,Walter W,Andrew A,Craig EA

更新日期：2003-11-25 00:00:00

abstract：:The release of urine, or micturition, serves a fundamental physiological function and, in many species, is critical for social communication. In mice, the pattern of urine release is modulated by external and internal factors and transmitted to the spinal cord via the pontine micturition center (PMC). Here, we exploit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyun M,Taranda J,Radeljic G,Miner L,Wang W,Ochandarena N,Huang KW,Osten P,Sabatini BL

更新日期：2021-01-05 00:00:00

abstract：:Negative elongation factor (NELF) and 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) are involved in pausing RNA Polymerase II (Pol II) in the promoter-proximal region of the hsp70 gene in Drosophila, before heat shock induction. Such blocks in elongation are widespread in the Dros...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Missra A,Gilmour DS

更新日期：2010-06-22 00:00:00

abstract：:Decomposition is a critical source of plant nutrients, and drives the largest flux of terrestrial C to the atmosphere. Decomposing soil organic matter typically contains litter from multiple plant species, yet we lack a mechanistic understanding of how species diversity influences decomposition processes. Here, we sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meier CL,Bowman WD

更新日期：2008-12-16 00:00:00

abstract：:We describe a Xenopus mRNA, Xrel1, that is related to the avian protooncogene c-rel, the embryonic pattern gene dorsal of Drosophila, and the mammalian transcription factor NK-kappa B/KBF1. The sequence of Xrel1 is homologous to the other rel-related proteins in the large amino-terminal region that defines this class ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kao KR,Hopwood ND

更新日期：1991-04-01 00:00:00

abstract：:We investigate yeast sex chromosome evolution by comparing genome sequences from 16 species in the family Saccharomycetaceae, including data from genera Tetrapisispora, Kazachstania, Naumovozyma, and Torulaspora. We show that although most yeast species contain a mating-type (MAT) locus and silent HML and HMR loci str...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon JL,Armisén D,Proux-Wéra E,ÓhÉigeartaigh SS,Byrne KP,Wolfe KH

更新日期：2011-12-13 00:00:00

abstract：:Dysregulation of cyclin-dependent kinase 5 (cdk5) per relative concentrations of its activators p35 and p25 is implicated in neurodegenerative diseases. P35 has a short t½ and undergoes rapid proteasomal degradation in its membrane-bound myristoylated form. P35 is converted by calpain to p25, which, along with an exte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai SY,Pokrass MJ,Klauer NR,Nohara H,Su TP

更新日期：2015-05-26 00:00:00

abstract：:Oxidants, such as those generated by metabolically activated phagocytes in inflammation, have been implicated in the metabolic activation of carcinogens, and in this study we demonstrate that the interaction of (+/-)-trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (BP 7,8-dihydrodiol) with phorbol ester-stimulated polym...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trush MA,Seed JL,Kensler TW

更新日期：1985-08-01 00:00:00

abstract：:The importance of gene gain through duplication has long been appreciated. In contrast, the importance of gene loss has only recently attracted attention. Indeed, studies in organisms ranging from plants to worms and humans suggest that duplication of some genes might be better tolerated than that of others. Here we h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Smet R,Adams KL,Vandepoele K,Van Montagu MC,Maere S,Van de Peer Y

更新日期：2013-02-19 00:00:00

abstract：:Corticosteroid hormones are critical for controlling metabolism, hydromineral balance, and the stress response in vertebrates. Although corticosteroid hormones have been well characterized in most vertebrate groups, the identity of the earliest vertebrate corticosteroid hormone has remained elusive. Here we provide ev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Close DA,Yun SS,McCormick SD,Wildbill AJ,Li W

更新日期：2010-08-03 00:00:00

abstract：:I reject the Darwinian assumption that larvae and their adults evolved from a single common ancestor. Rather I posit that, in animals that metamorphose, the basic types of larvae originated as adults of different lineages, i.e., larvae were transferred when, through hybridization, their genomes were acquired by distan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williamson DI

更新日期：2009-11-24 00:00:00

abstract：:In order to study the mutagenic effects of site-specific, covalent modifications of biologically active DNA, we need host cells that are permissive for any type of mutation that might be produced in vivo from the modified DNA. Specifically, we require a general, in vivo complementation system for the bacteriophage phi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Humayun MZ,Chambers RW

更新日期：1978-02-01 00:00:00

abstract：:The Ig new antigen receptors (IgNARs) are single-domain antibodies found in the serum of sharks. Here, we report 2.2- and 2.8-A structures of the type 2 IgNAR variable domains 12Y-1 and 12Y-2. Structural features include, first, an Ig superfamily topology transitional between cell adhesion molecules, antibodies, and T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Streltsov VA,Varghese JN,Carmichael JA,Irving RA,Hudson PJ,Nuttall SD

更新日期：2004-08-24 00:00:00

abstract：:Plasmodium vivax uses a single member of the Duffy binding-like (DBL) receptor family to invade erythrocytes and is not found in West Africa where its erythrocyte ligand, the Duffy blood group antigen, is missing. In contrast, Plasmodium falciparum expresses four members of the DBL family, and remarkably, single-point...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayer DC,Jiang L,Achur RN,Kakizaki I,Gowda DC,Miller LH

更新日期：2006-02-14 00:00:00

abstract：:Organic matter is responsible for the generation of hydrocarbons during the thermal maturation of source rock formation. This geochemical process engenders a network of organic hosted pores that governs the flow of hydrocarbons from the organic matter to fractures created during the stimulation of production wells. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berthonneau J,Obliger A,Valdenaire PL,Grauby O,Ferry D,Chaudanson D,Levitz P,Kim JJ,Ulm FJ,Pellenq RJ

更新日期：2018-12-04 00:00:00

abstract：:Intrathymic T-cell development requires temporally regulated rearrangement and expression of T-cell receptor (TCR) genes. To assess the role of the TCR beta gene transcriptional enhancer (Ebeta) in this process, mouse strains in which Ebeta is deleted were generated using homologous recombination techniques. We report...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bouvier G,Watrin F,Naspetti M,Verthuy C,Naquet P,Ferrier P

更新日期：1996-07-23 00:00:00

abstract：:The leukocyte common antigen (CD45) is an abundant lymphocyte surface antigen that has been reported to be involved in signaling through the T-cell antigen receptor. CD45 is a transmembrane protein-tyrosine-phosphatase. An internal segment comprises two domains each of which is homologous to other protein-tyrosine-pho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stover DR,Charbonneau H,Tonks NK,Walsh KA

更新日期：1991-09-01 00:00:00

abstract：:The cofactor composition and electron-transfer kinetics of the reaction center (RC) from a magnesium chelatase (bchD) mutant of Rhodobacter sphaeroides were characterized. In this RC, the special pair (P) and accessory (B) bacteriochlorophyll (BChl) -binding sites contain Zn-BChl rather than BChl a. Spectroscopic meas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin S,Jaschke PR,Wang H,Paddock M,Tufts A,Allen JP,Rosell FI,Mauk AG,Woodbury NW,Beatty JT

更新日期：2009-05-26 00:00:00

abstract：:IDIOTYPY HAS BEEN STUDIED IN ANTIBODIES AGAINST A PROTEIN ANTIGEN: hen ovalbumin. Various fractions of antisera to hen ovalbumin have been compared from the standpoint of the idiotypic specificities found on the immunoglobulins that they contain. Idiotypic specificities were found to be common to (a) antibodies that w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oudin J,Cazenave PA

更新日期：1971-10-01 00:00:00

abstract：:Deregulated production of nitric oxide (NO) has been implicated in the development of certain human diseases, including cancer. We sought to assess the damaging potential of NO produced under long-term conditions through the development of a suitable model cell culture system. In this study, we report that when murine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhuang JC,Wogan GN

更新日期：1997-10-28 00:00:00

abstract：:We model experience-dependent plasticity in the cortical representation of whiskers (the barrel cortex) in normal adult rats, and in adult rats that were prenatally exposed to alcohol. Prenatal exposure to alcohol (PAE) caused marked deficits in experience-dependent plasticity in a cortical barrel-column. Cortical pla...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benusková L,Rema V,Armstrong-James M,Ebner FF

更新日期：2001-02-27 00:00:00

abstract：:We developed a method for rapidly generating thermostable enzyme variants. Our strategy is to introduce the gene coding for a given enzyme from a mesophilic organism into a thermophile, Bacillus stearothermophilus. Variants that retain the enzymatic activity at the higher growth temperatures of the thermophile are the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao H,McKenzie T,Hageman R

更新日期：1986-02-01 00:00:00

abstract：:McKay et al. [(1996) Science 273, 924-930] suggested that carbonate globules in the meteorite ALH84001 contained the fossil remains of Martian microbes. We have characterized a subpopulation of magnetite (Fe(3)O(4)) crystals present in abundance within the Fe-rich rims of these carbonate globules. We find these Martia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Keprta KL,Clemett SJ,Bazylinski DA,Kirschvink JL,McKay DS,Wentworth SJ,Vali H,Gibson EK Jr,McKay MF,Romanek CS

更新日期：2001-02-27 00:00:00

abstract：:In mammals, toxic electrolytes of endogenous and exogenous origin are excreted through the urine and bile. Before excretion, these compounds cross numerous cellular membranes in a transporter-mediated manner. However, the protein transporters involved in the final excretion step are poorly understood. Here, we show th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Otsuka M,Matsumoto T,Morimoto R,Arioka S,Omote H,Moriyama Y

更新日期：2005-12-13 00:00:00

abstract：:Sinuous patterns traced by fluid flows are a ubiquitous feature of physical landscapes on Earth, Mars, the volcanic floodplains of the Moon and Venus, and other planetary bodies. Typically discussed as a consequence of migration processes in meandering rivers, sinuosity is also expressed in channel types that show lit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lazarus ED,Constantine JA

更新日期：2013-05-21 00:00:00

abstract：:Double-stranded RNA (dsRNA) recently has been shown to give rise to genetic interference in Caenorhabditis elegans and also is likely to be the basis for phenotypic cosuppression in plants in certain instances. While constructing a plasmid vector for transfection of trypanosome cells, we serendipitously discovered tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ngô H,Tschudi C,Gull K,Ullu E

更新日期：1998-12-08 00:00:00

abstract：:We have studied the genetic interactions between mutant alleles in 12 genes, designated PER1-PER12, which are essential for peroxisome biogenesis in the yeast Hansenula polymorpha. Recessive mutations in any of these genes determined three different morphological phenotypes: (i) complete absence of peroxisomes (Per-);...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Titorenko VI,Waterham HR,Cregg JM,Harder W,Veenhuis M

更新日期：1993-08-15 00:00:00

abstract：:IFN-alpha/beta plays an essential role in innate immunity against viral and bacterial infection. Among the proteins induced by IFN-alpha/beta are the ubiquitin-like ISG15 protein and its E1- (Ube1L) and E2- (UbcH8) conjugating enzymes, leading to the conjugation of ISG15 to cellular proteins. It is likely that ISG15 c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao C,Denison C,Huibregtse JM,Gygi S,Krug RM

更新日期：2005-07-19 00:00:00