Abstract:
:Expression of the Arabidopsis TRANSPARENT TESTA 2 (TT2) MYB family transcription factor leads to massive accumulation of proanthocyanidins (PAs) in hairy roots of Medicago truncatula. Microarray analysis showed that TT2 induces genes for flavonoid/PA biosynthesis, transcription factors, and a large number of genes of unknown function. A second microarray dataset identified genes that were preferentially expressed in the M. truncatula seed coat. Comparison of the two datasets defines target genes for steps that are yet unidentified in PA biosynthesis and accumulation. Of these genes, a glycosyltransferase, UGT72L1, was active specifically toward the PA precursor (-)-epicatechin, and its expression pattern in developing seeds correlated with the presence of epicatechin glucoside and accumulation of PAs. UGT72L1 may be involved in the production of epicatechin 3'-O-glucoside in the seed coat as a key step in PA biosynthesis or its regulation.
journal_name
Proc Natl Acad Sci U S Aauthors
Pang Y,Peel GJ,Sharma SB,Tang Y,Dixon RAdoi
10.1073/pnas.0805954105subject
Has Abstractpub_date
2008-09-16 00:00:00pages
14210-5issue
37eissn
0027-8424issn
1091-6490pii
0805954105journal_volume
105pub_type
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