Abstract:
:To understand molecular interactions that organize developing myofibrils, we examined the biosynthesis and interaction of titin and myosin heavy chain in cultures of developing muscle. Use of pulse-labeling, immunoprecipitation, and a reversible cross-linking procedure demonstrates that within minutes of synthesis, titin and myosin heavy chain can be chemically cross-linked into very large, detergent-resistant complexes retaining many features of intact myotubes. These complexes, predominantly of titin and myosin, occur very early in myofibrillogenesis as well as later. These data suggest that synthesis and assembly of titin and myosin are temporally and spatially coordinated in nascent myofibrils and support the hypothesis that titin molecules help to organize sarcomere formation.
journal_name
Proc Natl Acad Sci U S Aauthors
Isaacs WB,Kim IS,Struve A,Fulton ABdoi
10.1073/pnas.89.16.7496keywords:
subject
Has Abstractpub_date
1992-08-15 00:00:00pages
7496-500issue
16eissn
0027-8424issn
1091-6490journal_volume
89pub_type
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