Specificity in the activation and control of transcription factor E2F-dependent apoptosis.

abstract：:Osmotically shocked Escherichia coli and membrane vesicle ghosts from E. coli cells have lost the ability to accumulate potassium by active transport. The addition of valinomycin to the membrane ghosts restores the capacity to accumulate radioactive (42)K and (86)Rb by a temperature- and energy-dependent process. Memb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhattacharyya P,Epstein W,Silver S

更新日期：1971-07-01 00:00:00

abstract：:In this report, we explore the nature of the inductive stimuli leading to expression of the divergently regulated lymphokines interleukin 2 (IL-2) and interleukin 4 (IL-4). Elevation of cAMP levels blocks IL-2 induction while sparing IL-4 induction. These effects are gene-specific, not cell-specific, and can be observ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novak TJ,Rothenberg EV

更新日期：1990-12-01 00:00:00

abstract：:Glu-126 (helix IV) and Arg-144 (helix V) are charge paired and play a critical role in substrate binding in the lactose permease of Escherichia coli. When Glu-126 is replaced with Asp, the permease has relatively high activity, implying that helix V has sufficient flexibility to allow Arg-144 to accommodate the decrea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinglass AB,Kaback HR

更新日期：1999-09-28 00:00:00

abstract：:Epidermal growth factor (EGF) receptor monomers and noncovalently associated dimers were isolated by sucrose density gradient centrifugation, and their respective binding and autophosphorylation activities were determined. We find that monomers are low-affinity receptors and dimers are high-affinity receptors. In the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Böni-Schnetzler M,Pilch PF

更新日期：1987-11-01 00:00:00

abstract：:Real-life decisions are often repeated. Whether considering taking a job in a new city, or doing something mundane like checking if the stove is off, decisions are frequently revisited even if no new information is available. This mode of behavior takes a particularly pathological form in obsessive-compulsive disorder...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Solway A,Lin Z,Vinaik E

更新日期：2021-01-05 00:00:00

abstract：:Reverse transcriptase (RNA-directed DNA polymerase, EC 2.7.7.49) of human immunodeficiency virus type 1 has been examined with respect to the steady-state kinetics of polymerization of dNTPs into product DNA. With dNTPs as variable substrate, the kinetics of polymerization deviated from standard Michaelis-Menten kinet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： West AB,Roberts TM,Kolodner RD

更新日期：1992-10-15 00:00:00

abstract：:Our previous work [Levinger, L. & Varshavsky, A. (1982) Cell 28, 375-385] has shown that D1, a 50-kilodalton chromosomal protein of Drosophila melanogaster, is specifically associated with isolated nucleosomes that contain a complex A + T-rich satellite DNA with buoyant density of 1.688 g/ml. We show here that D1 is a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levinger L,Varshavsky A

更新日期：1982-12-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) undergo self-renewal divisions to support spermatogenesis throughout life. Although several positive regulators of SSC self-renewal have been discovered, little is known about the negative regulators. Here, we report that F-box and WD-40 domain protein 7 (FBXW7), a component of the Skp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanatsu-Shinohara M,Onoyama I,Nakayama KI,Shinohara T

更新日期：2014-06-17 00:00:00

abstract：:The adzuki bean beetle, Callosobruchus chinensis, is triple-infected with distinct lineages of Wolbachia endosymbiont, wBruCon, wBruOri, and wBruAus, which were identified by their wsp (Wolbachia surface protein) gene sequences. Whereas wBruCon and wBruOri caused cytoplasmic incompatibility of the host insect, wBruAus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo N,Nikoh N,Ijichi N,Shimada M,Fukatsu T

更新日期：2002-10-29 00:00:00

abstract：:As a first step in assessing the efficacy of a gene transfer approach to the induction of transplantation tolerance in our miniature swine model, double-copy retroviral vectors engineered to express a drug-resistance marker (neomycin) and a swine class II DRB cDNA were constructed. Infectious particles containing thes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shafer GE,Emery DW,Gustafsson K,Germana S,Anderson WF,Sachs DH,LeGuern C

更新日期：1991-11-01 00:00:00

abstract：:We recently have shown that mice deficient for the 86-kDa component (Ku80) of the DNA-dependent protein kinase exhibit growth retardation and a profound deficiency in V(D)J (variable, diversity, and joining) recombination. These defects may be related to abnormalities in DNA metabolism that arise from the inability of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nussenzweig A,Sokol K,Burgman P,Li L,Li GC

更新日期：1997-12-09 00:00:00

abstract：:Arguably the most foundational principle in perception research is that our experience of the world goes beyond the retinal image; we perceive the distal environment itself, not the proximal stimulation it causes. Shape may be the paradigm case of such "unconscious inference": When a coin is rotated in depth, we infer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morales J,Bax A,Firestone C

更新日期：2020-06-30 00:00:00

abstract：:A "supermarket revolution" has occurred in developing countries in the past 2 decades. We focus on three specific issues that reflect the impact of this revolution, particularly in Asia: continuity in transformation, innovation in transformation, and unique development strategies. First, the record shows that the rapi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reardon T,Timmer CP,Minten B

更新日期：2012-07-31 00:00:00

abstract：:Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a sy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tavenet A,Suleau A,Dubreuil G,Ferrari R,Ducrot C,Michaut M,Aude JC,Dieci G,Lefebvre O,Conesa C,Acker J

更新日期：2009-08-25 00:00:00

abstract：:A critical step in the induction of apoptosis is the activation of the apoptotic initiator caspase 9. We show that at its normal physiological concentration, caspase 9 is primarily an inactive monomer (zymogen), and that activity is associated with a dimeric species. At the high concentrations used for crystal formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renatus M,Stennicke HR,Scott FL,Liddington RC,Salvesen GS

更新日期：2001-12-04 00:00:00

abstract：:The gene V protein of bacteriophage f1 is a single-stranded DNA and RNA-binding protein composed of two identical subunits. We have constructed single-chain variants of the protein using short peptide linkers of five or six amino acids to connect the carboxyl terminus of one monomer to the amino terminus of the second...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang H,Sandberg WS,Terwilliger TC

更新日期：1993-08-01 00:00:00

abstract：:The effects of three anti-inflammatory drugs, which interfere with arachidonic acid transformation by three different enzymes, have been compared by using a simple model of tissue damage and foreign body rejection. In groups of control rats, subcutaneously implanted polyester sponges were rejected after a mean of 12 d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higgs GA,Mugridge KG,Moncada S,Vane JR

更新日期：1984-05-01 00:00:00

abstract：:The genes coding for apolipoproteins (apo) AI, CIII, and AIV, designated APOA1, APOC3, and APOA4, respectively, are closely linked and tandemly organized in the long arm of the human chromosome 11. A DNA rearrangement involving the genes encoding apoAI and apoCIII in certain patients with premature atherosclerosis has...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karathanasis SK,Ferris E,Haddad IA

更新日期：1987-10-01 00:00:00

abstract：:Epstein-Barr virus (EBV), an oncogenic herpesvirus, encodes two small RNAs (EBERs) that are expressed at high levels during latent transformation of human B lymphocytes. Here we report that a 15-kDa cellular protein called EAP (for EBER associated protein), previously shown to bind EBER1, is in fact the ribosomal prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toczyski DP,Matera AG,Ward DC,Steitz JA

更新日期：1994-04-12 00:00:00

abstract：:Members of the POU-homeodomain gene family encode transcriptional regulatory molecules that play important roles in terminal differentiation of many organ systems. Sperm-1 (Sprm-1) is a POU domain factor that is exclusively expressed in the differentiating male germ cell. We show here that the Sprm-1 protein is expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pearse RV 2nd,Drolet DW,Kalla KA,Hooshmand F,Bermingham JR Jr,Rosenfeld MG

更新日期：1997-07-08 00:00:00

abstract：:A cyclophilin (CyP) purified to homogeneity from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 had a molecular mass of 20.5 kDa and a pI of 8.1. The protein catalyzed the isomerization of the prolyl peptide bond of N-succinyl-Ala-Ala-(cis,trans)-Pro-Phe p-nitroanilide with a kcat/Km value of 9.3 x...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen H,Li XL,Ljungdahl LG

更新日期：1995-03-28 00:00:00

abstract：:The refractoriness of acute promyelocytic leukemia (APL) with t(11;17)(q23;q21) to all-trans retinoic acid (ATRA)-based therapy concerns clinicians and intrigues basic researchers. By using a murine leukemic model carrying both promyelocytic leukemia zinc finger/retinoic acid receptor-α (PLZF/RARα) and RARα/PLZF fusio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiao B,Ren ZH,Liu P,Chen LJ,Shi JY,Dong Y,Ablain J,Shi L,Gao L,Hu JP,Ren RB,de Thé H,Chen Z,Chen SJ

更新日期：2013-02-26 00:00:00

abstract：:In view of the extensive literature on phytochrome mutants in the Ler accession of Arabidopsis, we sought to secure a phytochrome-null line in the same genetic background for comparative studies. Here we report the isolation and phenotypic characterization of phyABCDE quintuple and phyABDE quadruple mutants in the Ler...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu W,Franklin KA,Sharrock RA,Jones MA,Harmer SL,Lagarias JC

更新日期：2013-01-22 00:00:00

abstract：:In this paper, we report an example of the engineered expression of tetrameric β-galactosidase (β-gal) containing varying numbers of active monomers. Specifically, by combining wild-type and single-nucleotide polymorphism plasmids at varying ratios, tetrameric β-gal was expressed in vitro with one to four active monom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Jiang Y,Chong S,Walt DR

更新日期：2018-08-14 00:00:00

abstract：:Although tumor necrosis factor (TNF) initially came to prominence because of its anti-tumor activity, most attention is now focused on its proinflammatory actions. TNF appears to play a critical role in both early and late events involved in inflammation, from localizing the noxious agent and amplifying the cellular a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marino MW,Dunn A,Grail D,Inglese M,Noguchi Y,Richards E,Jungbluth A,Wada H,Moore M,Williamson B,Basu S,Old LJ

更新日期：1997-07-22 00:00:00

abstract：:The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3' OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan J,Holzer S,Pellegrini L,Bell SD

更新日期：2018-06-26 00:00:00

abstract：:Although NMDA receptor (NMDAR)-dependent long-term potentiation (LTP) and long-term depression (LTD) of glutamatergic transmission are candidate mechanisms for long-term spatial memory, the precise contributions of LTP and LTD remain poorly understood. Here, we report that LTP and LTD in the hippocampal CA1 region of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ge Y,Dong Z,Bagot RC,Howland JG,Phillips AG,Wong TP,Wang YT

更新日期：2010-09-21 00:00:00

abstract：:Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

更新日期：1981-11-01 00:00:00

abstract：:As a second-order nonlinear optical process, sum-frequency generation is highly surface-specific and accordingly has been developed into a very powerful and versatile surface spectroscopic tool. It has found many unique applications in different disciplines and thus provided many exciting new research opportunities in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shen YR

更新日期：1996-10-29 00:00:00

abstract：:In movies or on TV, a wheel can seem to rotate backwards, due to the temporal subsampling inherent in the recording process (the wagon wheel illusion). Surprisingly, this effect has also been reported under continuous light, suggesting that our visual system, too, might sample motion in discrete "snapshots." Recently,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： VanRullen R,Reddy L,Koch C

更新日期：2005-04-05 00:00:00