当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Element-specific localization of Drosophila retrotransposon Gag proteins occurs in both nucleus and cytoplasm.

Element-specific localization of Drosophila retrotransposon Gag proteins occurs in both nucleus and cytoplasm.

Abstract：

:Many Drosophila non-long terminal repeat (LTR) retrotransposons actively transpose into internal, gene-rich regions of chromosomes but do not transpose onto chromosome ends. HeT-A and TART are remarkable exceptions; they form telomeres of Drosophila by repeated transpositions onto the ends of chromosomes and never transpose to internal regions of chromosomes. Both telomeric and nontelomeric, non-LTR elements transpose by target-primed reverse transcription, and their targets are not determined simply by DNA sequence, so it is not clear why these two kinds of elements have nonoverlapping transposition patterns. To explore roles of retrotransposon-encoded proteins in transposition, we analyzed intracellular targeting of Gag proteins from five non-LTR retrotransposons, HeT-A, TART, jockey, Doc, and I factor. All were expressed as green fluorescent protein-tagged proteins in cultured Drosophila cells. These Gag proteins have high levels of sequence similarity, but they have dramatic differences in intracellular targeting. As expected, HeT-A and TART Gags are transported efficiently to nuclei, where they show specific patterns of localization. These patterns are cell cycle-dependent, disappearing during mitosis. In contrast, only a fraction of jockey Gag moves into nuclei, whereas neither Doc nor I factor Gag is detected in the nucleus. Gags of the nontelomeric retrotransposons form characteristic clusters in the cytoplasm. These experiments demonstrate that closely related retrotransposon Gag proteins can have different intracellular localizations, presumably because they interact differently with cellular components. We suggest that these interactions reflect mechanisms by which the cell influences the level of transposition of an element.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Rashkova S,Karam SE,Pardue ML

doi

10.1073/pnas.032071999

keywords:

subject

Has Abstract

pub_date

2002-03-19 00:00:00

pages

3621-6

issue

6

eissn

0027-8424

issn

1091-6490

pii

032071999

journal_volume

99

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Molecular phylogeny of the extinct ground sloth Mylodon darwinii.

    abstract：:DNA was extracted from the remains of 35 ground sloths from various parts of North and South America. Two specimens of Mylodon darwinii, a species that went extinct at the end of the last glaciation, yielded amplifiable DNA. However, of the total DNA extracted, only approximately 1/1000 originated from the sloth, wher...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.1.181

    authors： Höss M,Dilling A,Currant A,Pääbo S

    更新日期：1996-01-09 00:00:00

  • Single molecule physics and chemistry.

    abstract：:New experiments using scanning probe microcopies and advanced optical methods allow us to study molecules as individuals, not just as populations. The findings of these studies not only include the confirmation of results expected from studies of bulk matter, but also give substantially new information concerning the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.20.11075

    authors： Bai C,Wang C,Xie XS,Wolynes PG

    更新日期：1999-09-28 00:00:00

  • Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins.

    abstract：:Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.16.8508

    authors： Anderson MT,Tjioe IM,Lorincz MC,Parks DR,Herzenberg LA,Nolan GP,Herzenberg LA

    更新日期：1996-08-06 00:00:00

  • Partitioning of atmospherically relevant ions between bulk water and the water/vapor interface.

    abstract：:Recently, surface-sensitive spectroscopy data and molecular dynamics simulations have generated intense interest in the distribution of electrolyte ions between bulk water and the air/water interface. A partitioning model for cations and anions developed for biopolymer surface is extended here to interpret the effects...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0606256103

    authors： Pegram LM,Record MT Jr

    更新日期：2006-09-26 00:00:00

  • HIV-1 gag recruits PACSIN2 to promote virus spreading.

    abstract：:The p2b domain of Rous sarcoma virus (RSV) Gag and the p6 domain of HIV-1 Gag contain late assembly (L) domains that engage the ESCRT membrane fission machinery and are essential for virus release. We now show that the PPXY-type RSV L domain specifically recruits the BAR domain protein PACSIN2 into virus-like particle...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1801849115

    authors： Popov S,Popova E,Inoue M,Wu Y,Göttlinger H

    更新日期：2018-07-03 00:00:00

  • Tertiary structure of myohemerythrin at low resolution.

    abstract：:X-ray diffraction studies have produced a low resolution image and also located the iron atoms of a monomeric hemerythrin from muscles of a sipunculan worm. These results reveal the course of the polypeptide chain and some details of the active center. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.6.2160

    authors： Hendrickson WA,Klippenstein GL,Ward KB

    更新日期：1975-06-01 00:00:00

  • Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products.

    abstract：:We have cloned and determined the entire nucleotide sequence of cDNAs corresponding to the putative alpha subunits of the human and rat mast cell high-affinity IgE receptors. Both human and rat cDNAs encode an NH2-terminal signal peptide, two immunoglobulin-like extracellular domains (encoded by discrete exons), a hyd...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.6.1907

    authors： Shimizu A,Tepler I,Benfey PN,Berenstein EH,Siraganian RP,Leder P

    更新日期：1988-03-01 00:00:00

  • Polyamine biosynthesis is required for the maintenance of peripheral blood cell elements in the rat.

    abstract：:The specific ornithine decarboxylase inhibitor alpha-difluoromethylornithine, when given to adult rats in vivo for 5 wk, resulted in a decrease in peripheral blood cell elements in normal rats and a marked suppression of marrow recovery in rats with chemotherapy-induced marrow hypoplasia. In normal rats, alpha-difluor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.16.5090

    authors： Luk GD,Sharkis SJ,Abeloff MD,McCann PP,Sjoerdsma A,Baylin SB

    更新日期：1983-08-01 00:00:00

  • Yeast mitochondrial ADP/ATP carriers are monomeric in detergents.

    abstract：:Mitochondrial carriers are believed widely to be homodimers both in the inner membrane of the organelle and in detergents. The dimensions and molecular masses of the detergent and protein-detergent micelles were measured for yeast ADP/ATP carriers in a range of different detergents. The radius of the carrier at the mi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0607640103

    authors： Bamber L,Harding M,Butler PJ,Kunji ER

    更新日期：2006-10-31 00:00:00

  • Association of integrase, matrix, and reverse transcriptase antigens of human immunodeficiency virus type 1 with viral nucleic acids following acute infection.

    abstract：:We have examined components of the preintegration complex of human immunodeficiency virus type 1 (HIV-1) and have analyzed features which govern the association of these components. HIV-1 nucleoprotein complexes, isolated from nuclear and cytoplasmic extracts of CD4+ cells after acute virus infection, contained viral ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.13.6125

    authors： Bukrinsky MI,Sharova N,McDonald TL,Pushkarskaya T,Tarpley WG,Stevenson M

    更新日期：1993-07-01 00:00:00

  • Genomic analysis of human and mouse TCL1 loci reveals a complex of tightly clustered genes.

    abstract：:TCL1 and TCL1b genes on human chromosome 14q23.1 are activated in T cell leukemias by translocations and inversions at 14q32.1, juxtaposing them to regulatory elements of T cell receptor genes. In this report we present the cloning, mapping, and expression analysis of the human and murine TCL1/Tcl1 locus. In addition ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.25.14418

    authors： Hallas C,Pekarsky Y,Itoyama T,Varnum J,Bichi R,Rothstein JL,Croce CM

    更新日期：1999-12-07 00:00:00

  • Ly49R activation receptor drives self-MHC-educated NK cell immunity against cytomegalovirus infection.

    abstract：:Natural killer (NK) cells mediate vital control of cancer and viral infection. They rely on MHC class I (MHC I)-specific self-receptors to identify and lyse diseased cells without harming self-MHC I-bearing host cells. NK cells bearing inhibitory self-receptors for host MHC I also undergo education, referred to as lic...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1913064117

    authors： Gamache A,Cronk JM,Nash WT,Puchalski P,Gillespie A,Wei H,Gray L,Hammarskjold ML,Xu W,Brown MG

    更新日期：2019-12-16 00:00:00

  • Intramolecular energy transfer and molecular conformation.

    abstract：:A discussion of the range of applicability of Forster long-range energy transfer in the determination of macromolecular dimensions and conformational dynamics is given. Emphasis is laid on the effect of restrictions in the orientational freedom of donor and acceptor and on the importance of the orientational averaging...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.2.271

    authors： Dale RE,Eisinger J

    更新日期：1976-02-01 00:00:00

  • Spectral completion of partially masked sounds.

    abstract：:Natural environments typically contain multiple sound sources. The sounds from these sources frequently overlap in time and often mask each other. Masking could potentially distort the representation of a sound's spectrum, altering its timbre and impairing object recognition. Here, we report that the auditory system p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0711291105

    authors： McDermott JH,Oxenham AJ

    更新日期：2008-04-15 00:00:00

  • Tyrosol is a quorum-sensing molecule in Candida albicans.

    abstract：:The human fungal pathogen Candida albicans shows a significant lag in growth when diluted into fresh minimal medium. This lag is abolished by the addition of conditioned medium from a high-density culture. The active component of conditioned medium is tyrosol, which is released into the medium continuously during grow...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0401416101

    authors： Chen H,Fujita M,Feng Q,Clardy J,Fink GR

    更新日期：2004-04-06 00:00:00

  • The frequency of polyploid speciation in vascular plants.

    abstract：:Since its discovery in 1907, polyploidy has been recognized as an important phenomenon in vascular plants, and several lines of evidence indicate that most, if not all, plant species ultimately have a polyploid ancestry. However, previous estimates of the frequency of polyploid speciation suggest that the formation an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0811575106

    authors： Wood TE,Takebayashi N,Barker MS,Mayrose I,Greenspoon PB,Rieseberg LH

    更新日期：2009-08-18 00:00:00

  • Isotype modulates epitope specificity, affinity, and antiviral activities of anti-HIV-1 human broadly neutralizing 2F5 antibody.

    abstract：:The constant heavy chain (CH1) domain affects antibody affinity and fine specificity, challenging the paradigm that only variable regions contribute to antigen binding. To investigate the role of the CH1 domain, we constructed IgA2 from the broadly neutralizing anti-HIV-1 2F5 IgG1, and compared 2F5 IgA2 and IgG bindin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1200024109

    authors： Tudor D,Yu H,Maupetit J,Drillet AS,Bouceba T,Schwartz-Cornil I,Lopalco L,Tuffery P,Bomsel M

    更新日期：2012-07-31 00:00:00

  • Borrowed alleles and convergence in serpentine adaptation.

    abstract：:Serpentine barrens represent extreme hazards for plant colonists. These sites are characterized by high porosity leading to drought, lack of essential mineral nutrients, and phytotoxic levels of metals. Nevertheless, nature forged populations adapted to these challenges. Here, we use a population-based evolutionary ge...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1600405113

    authors： Arnold BJ,Lahner B,DaCosta JM,Weisman CM,Hollister JD,Salt DE,Bomblies K,Yant L

    更新日期：2016-07-19 00:00:00

  • Tug-of-war between dissimilar teams of microtubule motors regulates transport and fission of endosomes.

    abstract：:Intracellular transport is interspersed with frequent reversals in direction due to the presence of opposing kinesin and dynein motors on organelles that are carried as cargo. The cause and the mechanism of reversals are unknown, but are a key to understanding how cargos are delivered in a regulated manner to specific...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0906524106

    authors： Soppina V,Rai AK,Ramaiya AJ,Barak P,Mallik R

    更新日期：2009-11-17 00:00:00

  • Visual responses of neurons in somatosensory cortex of hamsters with experimentally induced retinal projections to somatosensory thalamus.

    abstract：:These experiments investigate the capacity of thalamic and cortical structures in a sensory system to process information of a modality normally associated with another system. Retinal ganglion cells in newborn Syrian hamsters were made to project permanently to the main thalamic somatosensory (ventrobasal) nucleus. W...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.1.357

    authors： Métin C,Frost DO

    更新日期：1989-01-01 00:00:00

  • Dual-modality gene reporter for in vivo imaging.

    abstract：:The ability to track cells and their patterns of gene expression in living organisms can increase our understanding of tissue development and disease. Gene reporters for bioluminescence, fluorescence, radionuclide, and magnetic resonance imaging (MRI) have been described but these suffer variously from limited depth p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319000111

    authors： Patrick PS,Hammersley J,Loizou L,Kettunen MI,Rodrigues TB,Hu DE,Tee SS,Hesketh R,Lyons SK,Soloviev D,Lewis DY,Aime S,Fulton SM,Brindle KM

    更新日期：2014-01-07 00:00:00

  • Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs.

    abstract：:The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.14.5057

    authors： Su SS,Modrich P

    更新日期：1986-07-01 00:00:00

  • Inhibition of the MRP1-mediated transport of the menadione-glutathione conjugate (thiodione) in HeLa cells as studied by SECM.

    abstract：:Oxidative stress induced in live HeLa cells by menadione (2-methyl-1,4-napthaquinone) was studied in real time by scanning electrochemical microscopy (SECM). The hydrophobic molecule menadione diffuses through a living cell membrane where it is toxic to the cell. However, in the cell it is conjugated with glutathione ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1201555109

    authors： Koley D,Bard AJ

    更新日期：2012-07-17 00:00:00

  • Structure of the Leanyer orthobunyavirus nucleoprotein-RNA complex reveals unique architecture for RNA encapsidation.

    abstract：:Negative-stranded RNA viruses cover their genome with nucleoprotein (N) to protect it from the human innate immune system. Abrogation of the function of N offers a unique opportunity to combat the spread of the viruses. Here, we describe a unique fold of N from Leanyer virus (LEAV, Orthobunyavirus genus, Bunyaviridae ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1300035110

    authors： Niu F,Shaw N,Wang YE,Jiao L,Ding W,Li X,Zhu P,Upur H,Ouyang S,Cheng G,Liu ZJ

    更新日期：2013-05-28 00:00:00

  • Separating the effects of internal friction and transition state energy to explain the slow, frustrated folding of spectrin domains.

    abstract：:The elongated three-helix bundle domains spectrin R16 and R17 fold some two to three orders of magnitude more slowly than their homologue R15. We have shown that this slow folding is due, at least in part, to roughness in the free-energy landscape of R16 and R17. We have proposed that this roughness is due to a frustr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1201793109

    authors： Wensley BG,Kwa LG,Shammas SL,Rogers JM,Browning S,Yang Z,Clarke J

    更新日期：2012-10-30 00:00:00

  • Binding of Drosophila Polo kinase to its regulator Matrimony is noncanonical and involves two separate functional domains.

    abstract：:Drosophila melanogaster Polo kinase physically interacts with, and is repressed by, the Matrimony (Mtrm) protein during oogenesis. Females heterozygous for a deletion of the mtrm gene display defects in chromosome segregation at meiosis I. However, a complete absence of Mtrm results in both meiotic catastrophe and fem...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1301690110

    authors： Bonner AM,Hughes SE,Chisholm JA,Smith SK,Slaughter BD,Unruh JR,Collins KA,Friederichs JM,Florens L,Swanson SK,Pelot MC,Miller DE,Washburn MP,Jaspersen SL,Hawley RS

    更新日期：2013-03-26 00:00:00

  • ALS-linked mutant superoxide dismutase 1 (SOD1) alters mitochondrial protein composition and decreases protein import.

    abstract：:Mutations in superoxide dismutase 1 (SOD1) cause familial ALS. Mutant SOD1 preferentially associates with the cytoplasmic face of mitochondria from spinal cords of rats and mice expressing SOD1 mutations. Two-dimensional gels and multidimensional liquid chromatography, in combination with tandem mass spectrometry, rev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1014862107

    authors： Li Q,Vande Velde C,Israelson A,Xie J,Bailey AO,Dong MQ,Chun SJ,Roy T,Winer L,Yates JR,Capaldi RA,Cleveland DW,Miller TM

    更新日期：2010-12-07 00:00:00

  • Imprinted expression of genes and small RNA is associated with localized hypomethylation of the maternal genome in rice endosperm.

    abstract：:Arabidopsis thaliana endosperm, a transient tissue that nourishes the embryo, exhibits extensive localized DNA demethylation on maternally inherited chromosomes. Demethylation mediates parent-of-origin-specific (imprinted) gene expression but is apparently unnecessary for the extensive accumulation of maternally biase...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1306164110

    authors： Rodrigues JA,Ruan R,Nishimura T,Sharma MK,Sharma R,Ronald PC,Fischer RL,Zilberman D

    更新日期：2013-05-07 00:00:00

  • Developmental topography of cortical thickness during infancy.

    abstract：:During the first 2 postnatal years, cortical thickness of the human brain develops dynamically and spatially heterogeneously and likely peaks between 1 and 2 y of age. The striking development renders this period critical for later cognitive outcomes and vulnerable to early neurodevelopmental disorders. However, due t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1821523116

    authors： Wang F,Lian C,Wu Z,Zhang H,Li T,Meng Y,Wang L,Lin W,Shen D,Li G

    更新日期：2019-08-06 00:00:00

  • Structural basis for autoantibody recognition of phosphatidylserine-beta 2 glycoprotein I and apoptotic cells.

    abstract：:Apoptotic cells contain nuclear autoantigens that may initiate a systemic autoimmune response. To explore the mechanism of antibody binding to apoptotic cells, 3H9, a murine autoantibody with dual specificity for phospholipids and DNA, was used. H chain mutants of 3H9 were constructed, expressed as single-chain Fv (sc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.241510698

    authors： Cocca BA,Seal SN,D'Agnillo P,Mueller YM,Katsikis PD,Rauch J,Weigert M,Radic MZ

    更新日期：2001-11-20 00:00:00