Abstract:
:The ability to track cells and their patterns of gene expression in living organisms can increase our understanding of tissue development and disease. Gene reporters for bioluminescence, fluorescence, radionuclide, and magnetic resonance imaging (MRI) have been described but these suffer variously from limited depth penetration, spatial resolution, and sensitivity. We describe here a gene reporter, based on the organic anion transporting protein Oatp1a1, which mediates uptake of a clinically approved, Gd(3+)-based, hepatotrophic contrast agent (gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid). Cells expressing the reporter showed readily reversible, intense, and positive contrast (up to 7.8-fold signal enhancement) in T1-weighted magnetic resonance images acquired in vivo. The maximum signal enhancement obtained so far is more than double that produced by MRI gene reporters described previously. Exchanging the Gd(3+) ion for the radionuclide, (111)In, also allowed detection by single-photon emission computed tomography, thus combining the spatial resolution of MRI with the sensitivity of radionuclide imaging.
journal_name
Proc Natl Acad Sci U S Aauthors
Patrick PS,Hammersley J,Loizou L,Kettunen MI,Rodrigues TB,Hu DE,Tee SS,Hesketh R,Lyons SK,Soloviev D,Lewis DY,Aime S,Fulton SM,Brindle KMdoi
10.1073/pnas.1319000111subject
Has Abstractpub_date
2014-01-07 00:00:00pages
415-20issue
1eissn
0027-8424issn
1091-6490pii
1319000111journal_volume
111pub_type
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