Abstract:
:Over the past years, family 18 chitinases have been validated as potential targets for the design of drugs against human pathogens that contain or interact with chitin during their normal life cycles. Thus far, only one potent chitinase inhibitor has been described in detail, the pseudotrisaccharide allosamidin. Recently, however, two potent natural-product cyclopentapeptide chitinase inhibitors, argifin and argadin, were reported. Here, we describe high-resolution crystal structures that reveal the details of the interactions of these cyclopeptides with a family 18 chitinase. The structures are examples of complexes of a carbohydrate-processing enzyme with high-affinity peptide-based inhibitors and show in detail how the peptide backbone and side chains mimic the interactions of the enzyme with chitooligosaccharides. Together with enzymological characterization, the structures explain why argadin shows an order of magnitude stronger inhibition than allosamidin, whereas argifin shows weaker inhibition. The peptides bind to the chitinase in remarkably different ways, which may explain the differences in inhibition constants. The two complexes provide a basis for structure-based design of potent chitinase inhibitors, accessible by standard peptide chemistry.
journal_name
Proc Natl Acad Sci U S Aauthors
Houston DR,Shiomi K,Arai N,Omura S,Peter MG,Turberg A,Synstad B,Eijsink VG,van Aalten DMdoi
10.1073/pnas.132060599keywords:
subject
Has Abstractpub_date
2002-07-09 00:00:00pages
9127-32issue
14eissn
0027-8424issn
1091-6490pii
132060599journal_volume
99pub_type
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