Abstract:
:Global agricultural emissions of the greenhouse gas nitrous oxide (N2O) have increased by around 20% over the last 100 y, but regulation of these emissions and their impact on bacterial cellular metabolism are poorly understood. Denitrifying bacteria convert nitrate in soils to inert di-nitrogen gas (N2) via N2O and the biochemistry of this process has been studied extensively in Paracoccus denitrificans. Here we demonstrate that expression of the gene encoding the nitrous oxide reductase (NosZ), which converts N2O to N2, is regulated in response to the extracellular copper concentration. We show that elevated levels of N2O released as a consequence of decreased cellular NosZ activity lead to the bacterium switching from vitamin B12-dependent to vitamin B12-independent biosynthetic pathways, through the transcriptional modulation of genes controlled by vitamin B12 riboswitches. This inhibitory effect of N2O can be rescued by addition of exogenous vitamin B12.
journal_name
Proc Natl Acad Sci U S Aauthors
Sullivan MJ,Gates AJ,Appia-Ayme C,Rowley G,Richardson DJdoi
10.1073/pnas.1314529110subject
Has Abstractpub_date
2013-12-03 00:00:00pages
19926-31issue
49eissn
0027-8424issn
1091-6490pii
1314529110journal_volume
110pub_type
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