Hyper telomere recombination accelerates replicative senescence and may promote premature aging.

abstract：:We report on the production of tumor necrosis factor (TNF)-alpha and TNF-beta by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- and DQw1-positive donors frequently exhibit low production of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob CO,Fronek Z,Lewis GD,Koo M,Hansen JA,McDevitt HO

更新日期：1990-02-01 00:00:00

abstract：:The ribosomal RNA genes (rDNA) of Tetrahymena thermophila macronucleus exist as free linear 21-kilobase molecules that contain replication origins and telomeres. A mutation in this gene confers resistance to the antibiotic paromomycin. We have isolated rDNA from such a mutant (strain p2f), microinjected it into the ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tondravi MM,Yao MC

更新日期：1986-06-01 00:00:00

abstract：:Sialic acid-containing glycosphingolipids, i.e., gangliosides, constitute a major component of neuronal cells and are thought to be essential for brain function. UDP-glucose:ceramide glucosyltransferase (Ugcg) catalyzes the initial step of glycosphingolipid (GSL) biosynthesis. To gain insight into the role of GSLs in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jennemann R,Sandhoff R,Wang S,Kiss E,Gretz N,Zuliani C,Martin-Villalba A,Jäger R,Schorle H,Kenzelmann M,Bonrouhi M,Wiegandt H,Gröne HJ

更新日期：2005-08-30 00:00:00

abstract：:Approximately 25% of patients who are prescribed opioids for chronic pain misuse them, and 5 to 10% develop an opioid use disorder. Although the neurobiological target of opioids is well known, the molecular mechanisms that are responsible for the development of addiction-like behaviors in some but not all individuals...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kallupi M,Carrette LLG,Kononoff J,Solberg Woods LC,Palmer AA,Schweitzer P,George O,de Guglielmo G

更新日期：2020-01-28 00:00:00

abstract：:The Tol2 element of the medaka fish Oryzias latipes belongs to the hAT family of transposons (hobo/Ac/Tam3). We report here identification of a functional transposase of Tol2 that is capable of catalyzing its transposition in the germ line of zebrafish Danio rerio. A transcript produced from Tol2 encodes a putative tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawakami K,Shima A,Kawakami N

更新日期：2000-10-10 00:00:00

abstract：:The MoxR family of AAA+ ATPases is widespread throughout bacteria and archaea but remains poorly characterized. We recently found that the Escherichia coli MoxR protein, RavA (Regulatory ATPase variant A), tightly interacts with the inducible lysine decarboxylase, LdcI/CadA, to form a unique cage-like structure. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El Bakkouri M,Gutsche I,Kanjee U,Zhao B,Yu M,Goret G,Schoehn G,Burmeister WP,Houry WA

更新日期：2010-12-28 00:00:00

abstract：:Remarkable progress has come from whole-brain models linking anatomy and function. Paradoxically, it is not clear how a neuronal dynamical system running in the fixed human anatomical connectome can give rise to the rich changes in the functional repertoire associated with human brain function, which is impossible to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kringelbach ML,Cruzat J,Cabral J,Knudsen GM,Carhart-Harris R,Whybrow PC,Logothetis NK,Deco G

更新日期：2020-04-28 00:00:00

abstract：:The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. By using somatic cell hybrids and in situ chromosomal hybridization, we localized this gene to human chromosome 5 at bands q23-31, a chromos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Beau MM,Epstein ND,O'Brien SJ,Nienhuis AW,Yang YC,Clark SC,Rowley JD

更新日期：1987-08-01 00:00:00

abstract：:C-1027 is an enediyne antitumor antibiotic composed of four distinct moieties: an enediyne core, a deoxy aminosugar, a beta-amino acid, and a benzoxazolinate moiety. We now show that the benzoxazolinate moiety is derived from chorismate by the sequential action of two enzymes-SgcD, a 2-amino-2-deoxyisochorismate (ADIC...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Lanen SG,Lin S,Shen B

更新日期：2008-01-15 00:00:00

abstract：:Past research found that the ingestion of glucose can enhance self-control. It has been widely assumed that basic physiological processes underlie this effect. We hypothesized that the effect of glucose also depends on people's theories about willpower. Three experiments, both measuring (experiment 1) and manipulating...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Job V,Walton GM,Bernecker K,Dweck CS

更新日期：2013-09-10 00:00:00

abstract：:We report here on the isolation and characterization of a serotonin (5HT) transporter from Drosophila melanogaster. A 3.1-kb complementary DNA clone (dSERT) was found to encode a protein of 622 amino acid residues with a predicted molecular mass of approximately 69 kDa and a putative transmembrane topology characteris...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Demchyshyn LL,Pristupa ZB,Sugamori KS,Barker EL,Blakely RD,Wolfgang WJ,Forte MA,Niznik HB

更新日期：1994-05-24 00:00:00

abstract：:FRET between the zinc porphyrin (ZnP) chromophore in zinc-substituted cytochrome c (Zn-cyt c) and an Alexa Fluor dye attached to specific surface sites was used to characterize Zn-cyt c unfolding. The use of ZnP as a fluorescent acceptor eliminates the need to doubly label the protein with exogenous dyes to perform FR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ensign AA,Jo I,Yildirim I,Krauss TD,Bren KL

更新日期：2008-08-05 00:00:00

abstract：:The E6 oncoprotein of human papillomaviruses (HPVs) that are associated with cervical cancer utilizes the cellular ubiquitin-protein ligase E6-AP to target the tumor suppressor p53 for degradation. In normal cells (i.e., in the absence of E6), p53 is also a target of the ubiquitin-proteasome pathway. Under these condi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hengstermann A,Linares LK,Ciechanover A,Whitaker NJ,Scheffner M

更新日期：2001-01-30 00:00:00

abstract：:We have developed Differential Specificity and Energy Landscape (DiSEL) analysis to comprehensively compare DNA-protein interactomes (DPIs) obtained by high-throughput experimental platforms and cutting edge computational methods. While high-affinity DNA binding sites are identified by most methods, DiSEL uncovered nu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhimsaria D,Rodríguez-Martínez JA,Pan J,Roston D,Korkmaz EN,Cui Q,Ramanathan P,Ansari AZ

更新日期：2018-11-06 00:00:00

abstract：:The blue-light receptor genes (sopII) of sensory rhodopsin (SR) II were cloned from two species, the halophilic bacteria Haloarcula vallismortis (vSR-II) and Natronobacterium pharaonis (pSR-II). Upstream of both sopII gene loci, sequences corresponding to the halobacterial transducer of rhodopsin (Htr) II were recogni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seidel R,Scharf B,Gautel M,Kleine K,Oesterhelt D,Engelhard M

更新日期：1995-03-28 00:00:00

abstract：:Embryonic stem cell-based therapies exhibit great potential for the treatment of Parkinson's disease (PD) because they can significantly rescue PD-like behaviors. However, whether the transplanted cells themselves release dopamine in vivo remains elusive. We and others have recently induced human embryonic stem cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang X,Xu H,Teng S,Zhang X,Deng Z,Zhou L,Zuo P,Liu B,Liu B,Wu Q,Wang L,Hu M,Dou H,Liu W,Zhu F,Li Q,Guo S,Gu J,Lei Q,Lü J,Mu Y,Jin M,Wang S,Jiang W,Liu K,Wang C,Li W,Zhang K,Zhou Z

更新日期：2014-11-04 00:00:00

abstract：:125I-Labeled L-fucose-albumin complex and rat preputial beta-glucuronidase are rapidly cleared from plasma after intravenous infusion. L-Fucose-albumin retards the plasma clearance of beta-glucuronidase whereas D-fucose-albumin is inactive. In vitro, 125I-labeled L-fucose-albumin is taken up into rat or rabbit alveola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shepherd VL,Lee YC,Schlesinger PH,Stahl PD

更新日期：1981-02-01 00:00:00

abstract：:Unconscious neural activity has been repeatedly shown to precede and potentially even influence subsequent free decisions. However, to date, such findings have been mostly restricted to simple motor choices, and despite considerable debate, there is no evidence that the outcome of more complex free decisions can be pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soon CS,He AH,Bode S,Haynes JD

更新日期：2013-04-09 00:00:00

abstract：:The SRC gene is the prototype for a family of closely related genes whose products have protein-tyrosine kinase activity. We recently described another member of this family, designated FYN, whose cDNA was isolated from normal human fibroblasts. To examine the possible role of FYN as an oncogene, we investigated the e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawakami T,Kawakami Y,Aaronson SA,Robbins KC

更新日期：1988-06-01 00:00:00

abstract：:We examined human immunodeficiency virus (HIV) production in cultured mononuclear cells from 36 seropositive homosexual males. Production was detected by using an HIV p24 antigen ELISA assay in blood mononuclear cells in 54% of asymptomatic, 71% of acquired immunodeficiency syndrome (AIDS)-related complex, and 100% of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McElrath MJ,Pruett JE,Cohn ZA

更新日期：1989-01-01 00:00:00

abstract：:Adenosine triphosphate (ATP) is a regulatory molecule for many cell functions, both for intracellular and, perhaps less well known, extracellular functions. An important example of the latter involves red blood cells (RBCs), which help regulate blood pressure by releasing ATP as a vasodilatory signaling molecule in re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wan J,Ristenpart WD,Stone HA

更新日期：2008-10-28 00:00:00

abstract：:Organic substances can adopt an amorphous solid or semisolid state, influencing the rate of heterogeneous reactions and multiphase processes in atmospheric aerosols. Here we demonstrate how molecular diffusion in the condensed phase affects the gas uptake and chemical transformation of semisolid organic particles. Flo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shiraiwa M,Ammann M,Koop T,Pöschl U

更新日期：2011-07-05 00:00:00

abstract：:T cells are essential for immune defenses against pathogens, such that viability of naïve T cells before antigen encounter is critical to preserve a polyclonal repertoire and prevent immunodeficiencies. The viability of naïve T cells before antigen recognition is ensured by IL-7, which drives expression of the prosurv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller ML,Mashayekhi M,Chen L,Zhou P,Liu X,Michelotti M,Tramontini Gunn N,Powers S,Zhu X,Evaristo C,Alegre ML,Molinero LL

更新日期：2014-05-20 00:00:00

abstract：:Molecular dynamics simulations show that the desolvation rates of isotopes of Li(+), K(+), Rb(+), Ca(2+), Sr(2+), and Ba(2+) may have a relatively strong dependence on the metal cation mass. This inference is based on the observation that the exchange rate constant, k(wex), for water molecules in the first hydration s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hofmann AE,Bourg IC,DePaolo DJ

更新日期：2012-11-13 00:00:00

abstract：:DNA was prepared from 15 different mouse and rat cell lines transformed by chemical carcinogens in vitro and in vivo. These DNAs were applied to NIH3T3 mouse fibroblast cultures by using the calcium phosphate transfection technique. DNAs of five donor lines were able to induce foci on the recipient monolayers. Ten oth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shih C,Shilo BZ,Goldfarb MP,Dannenberg A,Weinberg RA

更新日期：1979-11-01 00:00:00

abstract：:African sleeping sickness is a fatal disease that is caused by the protozoan parasite Trypanosoma brucei. Polyamine biosynthesis is an essential pathway in the parasite and is a validated drug target for treatment of the disease. S-adenosylmethionine decarboxylase (AdoMetDC) catalyzes a key step in polyamine biosynthe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willert EK,Fitzpatrick R,Phillips MA

更新日期：2007-05-15 00:00:00

abstract：:von Willebrand factor (vWF) is a multimeric glycoprotein that mediates the adhesion of platelets to the subendothelium by binding to platelet glycoprotein Ib. For human vWF, this interaction can be induced in vitro by the antibiotic ristocetin or the snake venom protein botrocetin. A missense mutation, Gly-561-->Ser, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rabinowitz I,Tuley EA,Mancuso DJ,Randi AM,Firkin BG,Howard MA,Sadler JE

更新日期：1992-10-15 00:00:00

abstract：:The downstream box (DB) is a sequence element that enhances translation of several bacterial and phage mRNAs. It has been proposed that the DB enhances translation by base pairing transiently to bases 1469-1483 of 16S rRNA, the so-called anti-DB, during the initiation phase of translation. We have tested this model of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Connor M,Asai T,Squires CL,Dahlberg AE

更新日期：1999-08-03 00:00:00

abstract：:Worldwide dissemination of antibiotic resistance in bacteria is facilitated by plasmids that encode postsegregational killing (PSK) systems. These produce a stable toxin (T) and a labile antitoxin (A) conditioning cell survival to plasmid maintenance, because only this ensures neutralization of toxicity. Shortage of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pimentel B,Nair R,Bermejo-Rodríguez C,Preston MA,Agu CA,Wang X,Bernal JA,Sherratt DJ,de la Cueva-Méndez G

更新日期：2014-02-18 00:00:00

abstract：:Triglyceridemic response to a standard oral fat meal was determined in 28 healthy subjects and related to the levels of several lipids, lipoproteins, and apolipoproteins in the post-absorptive plasma. A fatty test meal was administered orally, and postprandial plasma triglyceride levels were determined. In the fasting...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patsch JR,Karlin JB,Scott LW,Smith LC,Gotto AM Jr

更新日期：1983-03-01 00:00:00