Abstract:
:We have compared chicken erythrocyte linker histones H1 and H5 binding to a synthetic four-way DNA junction. Each histone binds to form a single complex, with an affinity which permits competition against a large excess of linear duplex DNA. The affinity of H5 is higher than that of H1. The globular domain from either protein will also bind strongly, but in this case multiple binding occurs. Binding of intact H1 is inhibited by cations: Mg2+ and spermidine are very effective, Na+ much less so. This inhibition is not likely to be a general ion-competition effect, for Mg2+ is much less effective in inhibiting the binding of H1 to linear DNA. Instead, the inhibition of binding may be due to ion-dependent changes in the conformation of the four-way junction, which are known to occur under similar conditions. These results strongly suggest that the angle formed between the arms of the DNA junction could be a major determinant in the interaction of H1 with DNA crossovers.
journal_name
Proc Natl Acad Sci U S Aauthors
Varga-Weisz P,Zlatanova J,Leuba SH,Schroth GP,van Holde Kdoi
10.1073/pnas.91.9.3525subject
Has Abstractpub_date
1994-04-26 00:00:00pages
3525-9issue
9eissn
0027-8424issn
1091-6490journal_volume
91pub_type
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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doi:10.1073/pnas.1216824110
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2011-12-06 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1973-05-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1970-10-01 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:2020-01-07 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
pub_type: 杂志文章
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更新日期:2011-07-26 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1992-11-01 00:00:00