Structure of the Leanyer orthobunyavirus nucleoprotein-RNA complex reveals unique architecture for RNA encapsidation.

abstract：:Mutations responsible for inherited disease may act by disrupting normal transcriptional splicing. Such mutations can be difficult to detect, and their effects difficult to characterize, because many lie deep within exons or introns where they may alter splice enhancers or silencers or introduce new splice acceptors o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casadei S,Gulsuner S,Shirts BH,Mandell JB,Kortbawi HM,Norquist BS,Swisher EM,Lee MK,Goldberg Y,O'Connor R,Tan Z,Pritchard CC,King MC,Walsh T

更新日期：2019-12-16 00:00:00

abstract：:The overall structure of the transition-state and intermediate ensembles observed experimentally for dihydrofolate reductase and interleukin-1beta can be obtained by using simplified models that have almost no energetic frustration. The predictive power of these models suggests that, even for these very large proteins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clementi C,Jennings PA,Onuchic JN

更新日期：2000-05-23 00:00:00

abstract：:The initial electron transfer steps in the photosynthetic reaction center of the purple bacterium Rhodobacter sphaeroides have been investigated by femtosecond time-resolved spectroscopy. The experimental data taken at various wavelengths demonstrate the existence of at least four intermediate states within the first ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holzapfel W,Finkele U,Kaiser W,Oesterhelt D,Scheer H,Stilz HU,Zinth W

更新日期：1990-07-01 00:00:00

abstract：:This report describes an unbiased method for systematically determining gene function in mammalian cells. A total of 20,704 predicted human full-length cDNAs were tested for induction of the IL-8 promoter. A number of genes, including those for cytokines, receptors, adapters, kinases, and transcription factors, were i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iourgenko V,Zhang W,Mickanin C,Daly I,Jiang C,Hexham JM,Orth AP,Miraglia L,Meltzer J,Garza D,Chirn GW,McWhinnie E,Cohen D,Skelton J,Terry R,Yu Y,Bodian D,Buxton FP,Zhu J,Song C,Labow MA

更新日期：2003-10-14 00:00:00

abstract：:Most small multicopy antibiotic-resistance plasmids of Staphylococcus aureus contain a major axis of hyphenated dyad symmetry (palA) that is required for normal replication and stability, although located outside of the minimal replicon. Rearrangements affecting palA cause plasmid instability, a marked reduction in co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gruss AD,Ross HF,Novick RP

更新日期：1987-04-01 00:00:00

abstract：:Tomato plants overexpressing a prosystemin gene that encodes the precursor of a mobile wound signal called systemin have been shown previously to constitutively synthesize extraordinarily high levels of two defensive proteinase inhibitor proteins in leaves in the absence of wounding. We herein report that leaves of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Constabel CP,Bergey DR,Ryan CA

更新日期：1995-01-17 00:00:00

abstract：:A library of Photobacterium phosphoreum DNA was screened in lambda 2001 for the lumazine protein gene, using two degenerate 17-mer oligonucleotide probes that were deduced from a partial protein primary sequence. The lumazine protein gene was localized to a 3.4-kilobase BamHI/EcoRI fragment in one clone. The fragment ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Kane DJ,Woodward B,Lee J,Prasher DC

更新日期：1991-02-15 00:00:00

abstract：:Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 microM are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthesis. In the abse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JB

更新日期：1983-10-01 00:00:00

abstract：:Deletion of 5 residues (Delta5) from the central cytoplasmic loop of the lactose permease of Escherichia coli has no significant effect on expression or activity, whereas Delta12 leads to increased rates of permease turnover after membrane insertion and decreased transport activity, and Delta20 abolishes insertion and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinglass AB,Kaback HR

更新日期：2000-08-01 00:00:00

abstract：:The expansion of machine learning to high-stakes application domains such as medicine, finance, and criminal justice, where making informed decisions requires clear understanding of the model, has increased the interest in interpretable machine learning. The widely used Classification and Regression Trees (CART) have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luna JM,Gennatas ED,Ungar LH,Eaton E,Diffenderfer ES,Jensen ST,Simone CB 2nd,Friedman JH,Solberg TD,Valdes G

更新日期：2019-10-01 00:00:00

abstract：:We have studied the effect of the 3' terminal CCA sequence in precursors of tRNAs on catalysis by the RNase P RNA or the holoenzyme from the cyanobacterium Synechocystis sp. PCC 6803 in a completely homologous system. We have found that the absence of the 3' terminal CCA is not detrimental to activity, which is in sha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pascual A,Vioque A

更新日期：1999-06-08 00:00:00

abstract：:During cardiac trabeculation, cardiomyocytes delaminate from the outermost (compact) layer to form complex muscular structures known as trabeculae. As these cardiomyocytes delaminate, the remodeling of adhesion junctions must be tightly coordinated so cells can extrude from the compact layer while remaining in tight c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherian AV,Fukuda R,Augustine SM,Maischein HM,Stainier DY

更新日期：2016-07-05 00:00:00

abstract：:The uni1 mutant of Chlamydomonas reinhardtii lacks one of the paired flagella seen in wild-type cells. The missing flagellum is cis to the eye spot and at this site the basal body is incomplete, lacking a transition zone. Together with nine other loci affecting flagellar assembly and two loci affecting flagellar funct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramanis Z,Luck DJ

更新日期：1986-01-01 00:00:00

abstract：:To explore protein space from a global perspective, we consider 9,710 SCOP (Structural Classification of Proteins) domains with up to 70% sequence identity and present all similarities among them as networks: In the "domain network," nodes represent domains, and edges connect domains that share "motifs," i.e., signifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nepomnyachiy S,Ben-Tal N,Kolodny R

更新日期：2014-08-12 00:00:00

abstract：:Four synthetic peptides that together constitute the cell attachment domain of fibronectin [Pierschbacher, M.D., Ruoslahti, E., Sundelin, J., Lind, P. & Peterson, P. (1982) J. Biol. Chem. 257, 9593-9597] were constructed and tested for their ability to induce cell attachment and spreading. One of these peptides, consi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pierschbacher M,Hayman EG,Ruoslahti E

更新日期：1983-03-01 00:00:00

abstract：:VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. Here, the identification of an 83-kDa protein (p83) that specifically binds...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinhard M,Jouvenal K,Tripier D,Walter U

更新日期：1995-08-15 00:00:00

abstract：:Analysis of variations in the concentrations or structures of biomolecules (e.g., mRNAs, proteins, peptides, natural products) that occur either naturally or in response to environmental or genetic perturbations can provide important insight into complex biological processes. Many biological samples are mixtures that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao S,Anex DS,Caldwell WB,Arnold DW,Smith KB,Schultz PG

更新日期：1999-05-11 00:00:00

abstract：:The sperm receptors of the ascidian oocyte are located at the outer surface of the vitelline coat (formerly called the chorion). The fucose residues are the receptor's most important components for sperm recognition and binding. We asked whether the fucosyl-containing glycoproteins of the vitelline coat are a product ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosati F,Cotelli F,De Santis R,Monroy A,Pinto MR

更新日期：1982-03-01 00:00:00

abstract：:Mapping biological pathways across microbial genomes is a highly important technique in functional studies of biological systems. Existing methods mainly rely on sequence-based orthologous gene mapping, which often leads to suboptimal mapping results because sequence-similarity information alone does not contain suffi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao F,Su Z,Olman V,Dam P,Liu Z,Xu Y

更新日期：2006-01-03 00:00:00

abstract：:People who are more avoidant of pathogens are more politically conservative, as are nations with greater parasite stress. In the current research, we test two prominent hypotheses that have been proposed as explanations for these relationships. The first, which is an intragroup account, holds that these relationships ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tybur JM,Inbar Y,Aarøe L,Barclay P,Barlow FK,de Barra M,Becker DV,Borovoi L,Choi I,Choi JA,Consedine NS,Conway A,Conway JR,Conway P,Adoric VC,Demirci DE,Fernández AM,Ferreira DC,Ishii K,Jakšić I,Ji T,van Leeuwen

更新日期：2016-11-01 00:00:00

abstract：:Although sexual selection theory has proved successful in explaining a wide array of male ornaments, the function of ornaments occurring in females is largely unknown. Traditionally, female ornaments have been considered nonfunctional, being merely a genetically correlated response to selection for male ornamentation....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amundsen T,Forsgren E

更新日期：2001-11-06 00:00:00

abstract：:Emerging and reemerging viruses are responsible for a number of recent epidemic outbreaks. A crucial step in predicting and controlling outbreaks is the timely and accurate characterization of emerging virus strains. We present a portable microfluidic platform containing carbon nanotube arrays with differential filtra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeh YT,Gulino K,Zhang Y,Sabestien A,Chou TW,Zhou B,Lin Z,Albert I,Lu H,Swaminathan V,Ghedin E,Terrones M

更新日期：2020-01-14 00:00:00

abstract：:Porcine and bovine GH receptor (GHR) cDNAs were stably expressed in mouse L cells, which normally do not possess detectable levels of mouse GHR. Expression of the GHR cDNAs resulted in specific binding of 125I-labeled GH by these cell lines. To study GHR-related signaling events in these cells, protein tyrosine phosph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Xu B,Souza SC,Kopchick JJ

更新日期：1994-02-15 00:00:00

abstract：:Curved DNA molecules and unusually small circles have been obtained by ligation of synthetic 21-base precursors: (sequence in text). The ligation resulted in the formation of double-stranded oligo-(precursor)s possessing a strong 10.5-base-pair (bp) periodicity of the runs of adenines. Two-dimensional polyacrylamide g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulanovsky L,Bodner M,Trifonov EN,Choder M

更新日期：1986-02-01 00:00:00

abstract：:The ability of a specialized herbivore to overcome the chemical defense of a particular plant taxon not only makes it accessible as a food source but may also provide metabolites to be exploited for communication or chemical defense. Phyllotreta flea beetles are adapted to crucifer plants (Brassicales) that are defend...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beran F,Pauchet Y,Kunert G,Reichelt M,Wielsch N,Vogel H,Reinecke A,Svatoš A,Mewis I,Schmid D,Ramasamy S,Ulrichs C,Hansson BS,Gershenzon J,Heckel DG

更新日期：2014-05-20 00:00:00

abstract：:The global configuration of individual, surface-adsorbed molecules of the giant muscle protein titin, labeled with rhodamine conjugates, was followed with confocal microscopy. Fluorescence-emission intensity was reduced because of self-quenching caused by the close spacing between rhodamine dye molecules that formed d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grama L,Somogyi B,Kellermayer MS

更新日期：2001-12-04 00:00:00

abstract：:The latent membrane protein (LMP1) of Epstein-Barr virus (EBV) is expressed in EBV-associated nasopharyngeal carcinoma, which is notoriously metastatic. Although it is established that LMP1 represses E-cadherin expression and enhances the invasive ability of carcinoma cells, the mechanism underlying this repression re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai CN,Tsai CL,Tse KP,Chang HY,Chang YS

更新日期：2002-07-23 00:00:00

abstract：:Intestinal CD103(-) dendritic cells (DCs) are pathogenic for colitis. Unveiling molecular mechanisms that render these cells proinflammatory is important for the design of specific immunotherapies. In this report, we demonstrated that mesenteric lymph node CD103(-) DCs express, among other proinflammatory cytokines, h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kourepini E,Aggelakopoulou M,Alissafi T,Paschalidis N,Simoes DC,Panoutsakopoulou V

更新日期：2014-03-04 00:00:00

abstract：:Neurons respond to sensory stimuli by altering the rate and temporal pattern of action potentials. These spike trains both encode and propagate information that guides behavior. Local inhibitory networks can affect the information encoded and propagated by neurons by altering correlations between different spike train...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giridhar S,Doiron B,Urban NN

更新日期：2011-04-05 00:00:00

abstract：:Activation of androgen receptor (AR) via androgen in muscle cells has been closely linked to their growth and differentiation. Here, we report the cloning and characterization of supervillin (SV), a 205-kDa actin-binding protein, as an AR coregulator from the skeletal muscle cDNA library. Mammalian two-hybrid and glut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ting HJ,Yeh S,Nishimura K,Chang C

更新日期：2002-01-22 00:00:00