Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii.

abstract：:Linkage disequilibrium between polymorphisms in a natural population may result from various evolutionary forces, including random genetic drift due to sampling of gametes during reproduction, restricted migration between subpopulations in a subdivided population, or epistatic selection. In this report, we present evi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirby DA,Muse SV,Stephan W

更新日期：1995-09-26 00:00:00

abstract：:The global gene expression profiles for 67 human lung tumors representing 56 patients were examined by using 24,000-element cDNA microarrays. Subdivision of the tumors based on gene expression patterns faithfully recapitulated morphological classification of the tumors into squamous, large cell, small cell, and adenoc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garber ME,Troyanskaya OG,Schluens K,Petersen S,Thaesler Z,Pacyna-Gengelbach M,van de Rijn M,Rosen GD,Perou CM,Whyte RI,Altman RB,Brown PO,Botstein D,Petersen I

更新日期：2001-11-20 00:00:00

abstract：:A common complaint among pain patients is that they lose their appetite. These accounts are anecdotal, however, and the neural mechanism underlying pain-induced loss of appetite remains unknown. In this study, we documented the occurrence of appetite loss in patients under migraine attack and investigated the neuronal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malick A,Jakubowski M,Elmquist JK,Saper CB,Burstein R

更新日期：2001-08-14 00:00:00

abstract：:DNA heteroduplexes with single unpaired bases of the four different kinds were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system can reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dohet C,Wagner R,Radman M

更新日期：1986-05-01 00:00:00

abstract：:In a previous study we demonstrated that vesicular stomatitis virus (VSV) can be used as a vector to express a soluble protein in mammalian cells. Here we have generated VSV recombinants that express four different membrane proteins: the cellular CD4 protein, a CD4-G hybrid protein containing the ectodomain of CD4 and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schnell MJ,Buonocore L,Kretzschmar E,Johnson E,Rose JK

更新日期：1996-10-15 00:00:00

abstract：:The G12 subfamily of heterotrimeric G proteins, comprised of the alpha-subunits Galpha12 and Galpha13, has been implicated as a signaling component in cellular processes ranging from cytoskeletal changes to cell growth and oncogenesis. In an attempt to elucidate specific roles of this subfamily in cell regulation, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meigs TE,Fields TA,McKee DD,Casey PJ

更新日期：2001-01-16 00:00:00

abstract：:The oligopeptide permease of Escherichia coli has been characterized by Payne, Gilvarg, and their colleagues. We have confirmed its existence in Salmonella typhimurium, and have isolated a series of mutants lacking the permease. We use this transport system for smuggling a histidine biosynthetic intermediate, histidin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ames BN,Ames GF,Young JD,Tsuchiya D,Lecocq J

更新日期：1973-02-01 00:00:00

abstract：:Knowledge of normal human cardiac excitation stems from isolated heart or intraoperative mapping studies under nonphysiological conditions. Here, we use a noninvasive imaging modality (electrocardiographic imaging) to study normal activation and repolarization in intact unanesthetized healthy adults under complete phy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramanathan C,Jia P,Ghanem R,Ryu K,Rudy Y

更新日期：2006-04-18 00:00:00

abstract：:In vitro translation of Rous sarcoma virus (RSV) virion RNA in the messenger-dependent reticulocyte lysate system yielded polypeptides that were not synthesized by translation of RNA from a transformation-defective deletion mutant of RSV. These RSV-specific products migrated on sodium dodecyl sulfate/polyacrylamide ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beemon K,Hunter T

更新日期：1977-08-01 00:00:00

abstract：:The local conformations of proteins and peptides are determined by the amino acid sequence. However, the 20 amino acids encoded by the genome allow the peptide backbone to fold into many conformations, so that even for a small peptide it becomes very difficult to predict the three-dimensional structure. By using empir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burgess AW

更新日期：1994-03-29 00:00:00

abstract：:Hyperapobetalipoproteinemia is defined as the combination of a normal low density lipoprotein (LDL) cholesterol in the face of an increased LDL apolipoprotein B (apoB) protein. To examine the physical basis for the apparent disproportion between LDL cholesterol and apoB characteristic of this syndrome, we used density...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teng B,Thompson GR,Sniderman AD,Forte TM,Krauss RM,Kwiterovich PO Jr

更新日期：1983-11-01 00:00:00

abstract：:Abnormal H2O2 levels are closely related to many diseases, including inflammation and cancers. Herein, we simultaneously load HRP and its substrate, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), into liposomal nanoparticles, obtaining a Lipo@HRP&ABTS optical nanoprobe for in vivo H2O2-responsive chrom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Q,Liang C,Sun X,Chen J,Yang Z,Zhao H,Feng L,Liu Z

更新日期：2017-05-23 00:00:00

abstract：:The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frouws TD,Duda SC,Richmond TJ

更新日期：2016-02-02 00:00:00

abstract：:A MODEL OF THE LINEAR HYDROGEN BOND HAS BEEN DEVISED WHICH IS CHARACTERIZED BY THREE PHYSICAL PARAMETERS OF THE ISOLATED ELECTRON DONOR AND PROTON DONOR MOLECULES: mu(A-H), the bond dipole moment; DeltaI, the difference in ionization potential between that of the electron donor and the corresponding noble gas atom; an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Allen LC

更新日期：1975-12-01 00:00:00

abstract：:Changes in levels of the newly discovered cytidine 3':5'-cyclic monophosphate (cyclic CMP) phosphodiesterase in some representative tissues (cerebral cortex, kidney, intestine, liver, heart, and lung) of guinea pigs from various developmental stages (fetus, neonate, pup, and adult) were studied and compared with those...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Helfman DM,Brackett NL,Kuo JF

更新日期：1978-09-01 00:00:00

abstract：:Antibody-coated latex beads (Ab-beads) were attached to Leptospira interrogans serovars illini 3055 and icterohaemorrhagiae SC1157. The movement of the Ab-beads relative to the motion of the cells was observed by direct darkfield microscopy or was recorded on videotape. When the Ab-beads were attached to the front end...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charon NW,Lawrence CW,O'Brien S

更新日期：1981-11-01 00:00:00

abstract：:A full-length placental alkaline phosphatase (PLAP) cDNA was used to identify and clone the PLAP-like Nagao isozyme gene from human genomic libraries. The entire nucleotide sequence of the gene reveals the existence of 11 exons interrupted by 10 small introns (76-427 base pairs). Putative regulatory sequences have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millán JL,Manes T

更新日期：1988-05-01 00:00:00

abstract：:A randomized double-blind controlled trial was conducted to determine the efficacy of passive immunotherapy in the treatment of symptomatic human immunodeficiency virus (HIV) infection. This trial included 86 symptomatic patients randomized to receive plasma rich in anti-HIV-1 antibody or standard seronegative plasma....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vittecoq D,Chevret S,Morand-Joubert L,Heshmati F,Audat F,Bary M,Dusautoir T,Bismuth A,Viard JP,Barré-Sinoussi F

更新日期：1995-02-14 00:00:00

abstract：:The amphibian tetradecapeptide, bombesin (BBS) has been shown to stimulate insulin secretion both in vivo and by pancreatic islet cells in vitro. To determine whether BBS can act directly on pancreatic beta cells, we examined its effects on insulin secretion by HIT-T15 cells (HIT cells), a clonal islet cell line. Addi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swope SL,Schonbrunn A

更新日期：1984-03-01 00:00:00

abstract：:Parkinson's disease (PD) is a neurodegenerative disorder of uncertain pathogenesis characterized by a loss of substantia nigra pars compacta (SNpc) dopaminergic (DA) neurons, and can be modeled by the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Both inflammatory processes and oxidative stress may c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu DC,Teismann P,Tieu K,Vila M,Jackson-Lewis V,Ischiropoulos H,Przedborski S

更新日期：2003-05-13 00:00:00

abstract：:We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz O,Pieper C,Clever M,Pfaff J,Ruhlandt A,Kehlenbach RH,Wouters FS,Großhans J,Bunt G,Enderlein J

更新日期：2013-12-24 00:00:00

abstract：:Conjugative DNA transfer of the self-transmissible broad-host-range plasmid RP4 is initiated by strand- and site-specific cleavage at the nick site (nic) of the transfer origin (oriT). Cleavage results in covalent attachment of the plasmid-encoded relaxase (TraI) to the 5'-terminal 2'-deoxycytidine residue at nic. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pansegrau W,Schröder W,Lanka E

更新日期：1993-04-01 00:00:00

abstract：:We have investigated the role of cAMP in NO- and prostanoid-independent relaxations that are widely attributed to an endothelium-derived hyperpolarizing factor (EDHF). Under control conditions EDHF-type relaxations evoked by acetylcholine (ACh) in rabbit iliac arteries were transient, but in the presence of the cAMP p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffith TM,Chaytor AT,Taylor HJ,Giddings BD,Edwards DH

更新日期：2002-04-30 00:00:00

abstract：:Diabetic peripheral neuropathy (DPN) is the most common complication in both type 1 and type 2 diabetes. Here we studied some phenotypic features of a well-established animal model of type 2 diabetes, the leptin receptor-deficient db(-)/db(-) mouse, and also the effect of long-term (6 mo) treatment with coenzyme Q10 (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi TJ,Zhang MD,Zeberg H,Nilsson J,Grünler J,Liu SX,Xiang Q,Persson J,Fried KJ,Catrina SB,Watanabe M,Arhem P,Brismar K,Hökfelt TG

更新日期：2013-01-08 00:00:00

abstract：:Siglecs are a recently discovered family of animal lectins that belong to the Ig superfamily and recognize sialic acids (Sias). CD33-related Siglecs (CD33rSiglecs) are a subgroup with as-yet-unknown functions, characterized by sequence homology, expression on innate immune cells, conserved cytosolic tyrosine-based sig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angata T,Margulies EH,Green ED,Varki A

更新日期：2004-09-07 00:00:00

abstract：:The signals required for rapid internalization of the bovine cation-dependent mannose 6-phosphate receptor have been localized to two distinct regions of the 67-amino acid cytoplasmic domain. One signal includes phenylalanine 13 and phenylalanine 18, while the other involves tyrosine 45. The former signal is more pote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson KF,Chan W,Kornfeld S

更新日期：1990-12-01 00:00:00

abstract：:Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hollands K,Sevostiyanova A,Groisman EA

更新日期：2014-05-13 00:00:00

abstract：:A number of eukaryotic viruses have evolved mechanisms to downregulate activity of the interferon-induced, double-stranded RNA-activated protein kinase (referred to as P68 based on its Mr of 68,000 in human cells). This control is essential because once activated, the P68 kinase phosphorylates its natural substrate, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee TG,Tomita J,Hovanessian AG,Katze MG

更新日期：1990-08-01 00:00:00

abstract：:Linear double-stranded molecules of the circularly permuted and terminally redundant DNA of Salmonella bacteriophage P22 have been converted to oligomeric products in the presence of polynucleotide ligase coded for by the coliphage T4. The reaction has been monitored by sucrose density-gradient centrifugation and elec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sgaramella V

更新日期：1972-11-01 00:00:00

abstract：:Vesicles were constituted with glycophorin, the Sendai virus receptor of human erythrocytes, and loaded with calcein, a polar derivative of fluorescein, at self-quenching concentrations. On exposure to Sendai virus and mild hypo-osmotic stress, vesicles of the appropriate composition released a significant portion of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kundrot CE,Spangler EA,Kendall DA,MacDonald RC,MacDonald RI

更新日期：1983-03-01 00:00:00