Abstract:
:Analysis of variations in the concentrations or structures of biomolecules (e.g., mRNAs, proteins, peptides, natural products) that occur either naturally or in response to environmental or genetic perturbations can provide important insight into complex biological processes. Many biological samples are mixtures that require a separation step before quantitation of variations in the individual components. Two-dimensional denaturing gel electrophoresis has been used very effectively to separate complex mixtures of proteins, but it is time consuming and requires considerable amounts of sample. Microchannel-based separations have proven very effective in rapidly separating small amounts of nucleic acids; more recently, isoelectric focusing of proteins also has been adapted to the microchannel format. Here, we describe microchannel-based SDS capillary gel electrophoresis of proteins and demonstrate the speed and high resolution it provides. This development is an important step toward the miniaturization and integration of multidimensional and array separation methods for complex protein mixtures.
journal_name
Proc Natl Acad Sci U S Aauthors
Yao S,Anex DS,Caldwell WB,Arnold DW,Smith KB,Schultz PGdoi
10.1073/pnas.96.10.5372keywords:
subject
Has Abstractpub_date
1999-05-11 00:00:00pages
5372-7issue
10eissn
0027-8424issn
1091-6490journal_volume
96pub_type
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