Single molecule physics and chemistry.

abstract：:An improved mammalian two-hybrid system designed for interaction trap screening is described in this paper. CV-1/EBNA-1 monkey kidney epithelial cells expressing Epstein-Barr virus nuclear antigen 1 (EBNA-1) were stably transfected with a reporter plasmid for GAL4-dependent expression of the green fluorescent protein ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shioda T,Andriole S,Yahata T,Isselbacher KJ

更新日期：2000-05-09 00:00:00

abstract：:Smooth muscle cells use fibronectin to bind to type I and type III collagens but bind to type V collagen by a trypsin-resistant intrinsic glycoconjugate. The binding site on type V collagen is located in the alpha A chain. By using collagen-coated filters in a modified Boyden chamber assay for chemotaxis, it was obser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grotendorst GR,Seppä HE,Kleinman HK,Martin GR

更新日期：1981-06-01 00:00:00

abstract：:DNA mismatch repair (MMR), the guardian of the genome, commences when MutS identifies a mismatch and recruits MutL to nick the error-containing strand, allowing excision and DNA resynthesis. Dominant MMR models posit that after mismatch recognition, ATP converts MutS to a hydrolysis-independent, diffusive mobile clamp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hao P,LeBlanc SJ,Case BC,Elston TC,Hingorani MM,Erie DA,Weninger KR

更新日期：2020-07-28 00:00:00

abstract：:Trimeric class I virus fusion proteins undergo a series of conformational rearrangements that leads to the association of C- and N-terminal heptad repeat domains in a "trimer-of-hairpins" structure, facilitating the apposition of viral and cellular membranes during fusion. This final fusion hairpin structure is sustai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cianci C,Langley DR,Dischino DD,Sun Y,Yu KL,Stanley A,Roach J,Li Z,Dalterio R,Colonno R,Meanwell NA,Krystal M

更新日期：2004-10-19 00:00:00

abstract：:Bacterial virulence factors are typically surface-associated or secreted molecules that in Gram-negative bacteria must cross the outer membrane (OM). Protein translocation across the bacterial OM is not well understood. To elucidate this process we studied P pilus biogenesis in Escherichia coli. We present high-resolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thanassi DG,Saulino ET,Lombardo MJ,Roth R,Heuser J,Hultgren SJ

更新日期：1998-03-17 00:00:00

abstract：:Collective intelligence refers to the ability of groups to outperform individual decision makers when solving complex cognitive problems. Despite its potential to revolutionize decision making in a wide range of domains, including medical, economic, and political decision making, at present, little is known about the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kurvers RH,Herzog SM,Hertwig R,Krause J,Carney PA,Bogart A,Argenziano G,Zalaudek I,Wolf M

更新日期：2016-08-02 00:00:00

abstract：:We are designing simple peptide ion channels as model systems for the study of the physical principles controlling conduction through ion-channel proteins. Here we report on an uncharged peptide, Ac-(Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2, designed to form an aggregate of parallel, amphiphilic, membrane-spanning alpha-he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kienker PK,DeGrado WF,Lear JD

更新日期：1994-05-24 00:00:00

abstract：:A major barrier to the design of immunotherapeutics and vaccines for cancer is the idiosyncratic nature of many tumor antigens and the possibility that T cells may be tolerant of broadly distributed antigens. We have devised an experimental strategy that exploits species differences in protein sequences to circumvent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Theobald M,Biggs J,Dittmer D,Levine AJ,Sherman LA

更新日期：1995-12-19 00:00:00

abstract：:Using a water-based prebiotic model of sugar synthesis involving glycolaldehyde self-condensation, we demonstrate that homochiral L-dipeptide catalysts lead to the stereospecific syntheses of tetroses. The asymmetric effect is largest for erythrose, which may reach a D-enantiomeric excess of >80% with L-Val-L-Val cata...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weber AL,Pizzarello S

更新日期：2006-08-22 00:00:00

abstract：:A cylindrical detonating explosive behaves like a non-Newtonian viscous fluid emerging from a cylindrical pipe. The expression for non-Newtonian viscous flow has been applied to detonating explosives. The resultant fit to emerging flame fronts from detonation is excellent for a wide variety of detonating explosives bo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coon N,Ma SM,Redington PK,Brown B,Eyring H

更新日期：1980-10-01 00:00:00

abstract：:Despite the importance of valuing another person's welfare for prosocial behavior, currently we have only a limited understanding of how these values are represented in the brain and, more importantly, how they give rise to individual variability in prosociality. In the present study, participants underwent functional...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sul S,Tobler PN,Hein G,Leiberg S,Jung D,Fehr E,Kim H

更新日期：2015-06-23 00:00:00

abstract：:Seep sediments are dominated by intensive microbial sulfate reduction coupled to the anaerobic oxidation of methane (AOM). Through geochemical measurements of incubation experiments with methane seep sediments collected from Hydrate Ridge, we provide insight into the role of iron oxides in sulfate-driven AOM. Seep sed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sivan O,Antler G,Turchyn AV,Marlow JJ,Orphan VJ

更新日期：2014-10-07 00:00:00

abstract：:Highly multiplexed assays using antibody coated, fluorescent (xMap) beads are widely used to measure quantities of soluble analytes, such as cytokines and antibodies in clinical and other studies. Current analyses of these assays use methods based on standard curves that have limitations in detecting low or high abund...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Won JH,Goldberger O,Shen-Orr SS,Davis MM,Olshen RA

更新日期：2012-02-21 00:00:00

abstract：:In epithelia, a primary damage of tight junctions (TJ) always leads to a secondary disruption of adherens junction (AJ), and vice versa. This response, if occurring in the testis, would disrupt spermatogenesis because the blood-testis barrier (BTB) must remain intact during the transit of spermatids in the seminiferou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lie PP,Chan AY,Mruk DD,Lee WM,Cheng CY

更新日期：2010-06-22 00:00:00

abstract：:Photoadaptation, the ability to attenuate a light response on prolonged light exposure while remaining sensitive to escalating changes in light intensity, is essential for organisms to decipher time information appropriately, yet the underlying molecular mechanisms are poorly understood. In Neurospora crassa, VIVID (V...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen CH,DeMay BS,Gladfelter AS,Dunlap JC,Loros JJ

更新日期：2010-09-21 00:00:00

abstract：:Hypertonicity-induced binding of the transcription factor TonEBP/OREBP to its cognate DNA element, ORE/TonE, is associated with increased transcription of several osmotically regulated genes. Previously, it was found that hypertonicity rapidly causes nuclear translocation and phosphorylation of TonEBP/OREBP and, more ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferraris JD,Williams CK,Persaud P,Zhang Z,Chen Y,Burg MB

更新日期：2002-01-22 00:00:00

abstract：:The nuclear receptor REV-ERBα integrates the circadian clock with hepatic glucose and lipid metabolism by nucleating transcriptional comodulators at genomic regulatory regions. An interactomic approach identified O-GlcNAc transferase (OGT) as a REV-ERBα-interacting protein. By shielding cytoplasmic OGT from proteasoma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berthier A,Vinod M,Porez G,Steenackers A,Alexandre J,Yamakawa N,Gheeraert C,Ploton M,Maréchal X,Dubois-Chevalier J,Hovasse A,Schaeffer-Reiss C,Cianférani S,Rolando C,Bray F,Duez H,Eeckhoute J,Lefebvre T,Staels B,Lef

更新日期：2018-11-20 00:00:00

abstract：:Three components of differentiated muscle membrane, the acetylcholine receptor, acetylcholinesterase (EC 3.1.1.7; acetylcholine hydrolase), and adenylate cyclase [EC 4.6.1.1; ATP pyrophosphate-lyase (cyclizing)], appear simultaneously during myogenesis in cultures of embryonic chick muscle, after the main period of ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prives JM,Paterson BM

更新日期：1974-08-01 00:00:00

abstract：:Mass-independent isotope fractionations driven by differences in volumes and shapes of nuclei (the field shift effect) are known in several elements and are likely to be found in more. All-electron relativistic electronic structure calculations can predict this effect but at present are computationally intensive and l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schauble EA

更新日期：2013-10-29 00:00:00

abstract：:The effect of human papillomavirus (HPV) and activated oncogenes on the growth and morphology of primary baby mouse kidney (BMK) cells has been studied. Early region DNA from HPV types 16, 18, 31, and 33, but not type 6, under the transcriptional control of a heterologous, retroviral promoter cooperated with EJ-ras to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crook T,Storey A,Almond N,Osborn K,Crawford L

更新日期：1988-12-01 00:00:00

abstract：:The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a stra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turroni F,Bottacini F,Foroni E,Mulder I,Kim JH,Zomer A,Sánchez B,Bidossi A,Ferrarini A,Giubellini V,Delledonne M,Henrissat B,Coutinho P,Oggioni M,Fitzgerald GF,Mills D,Margolles A,Kelly D,van Sinderen D,Ventura M

更新日期：2010-11-09 00:00:00

abstract：:Beetle luciferases are thought to have evolved from fatty acyl-CoA synthetases present in all insects. Both classes of enzymes activate fatty acids with ATP to form acyl-adenylate intermediates, but only luciferases can activate and oxidize d-luciferin to emit light. Here we show that the Drosophila fatty acyl-CoA syn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mofford DM,Reddy GR,Miller SC

更新日期：2014-03-25 00:00:00

abstract：:The primary base sequence of the protein coding regions of the four small heat shock genes of Drosophila melanogaster present at cytological locus 67B has been determined. A single open reading frame large enough to encode a small heat shock protein is found for each gene. The molecular weights of the predicted protei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ingolia TD,Craig EA

更新日期：1982-04-01 00:00:00

abstract：:The ten-eleven translocation (TET) family of methylcytosine dioxygenases initiates demethylation of DNA and is associated with tumorigenesis in many cancers; however, the mechanism is mostly unknown. Here we identify upstream activators and downstream effectors of TET1 in breast cancer using human breast cancer cells ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun M,Song CX,Huang H,Frankenberger CA,Sankarasharma D,Gomes S,Chen P,Chen J,Chada KK,He C,Rosner MR

更新日期：2013-06-11 00:00:00

abstract：:In eukaryotes, microtubules are essential for cellular plasticity and dynamics. Here we show that P300/CBP-associated factor (PCAF), a kinetochore-associated acetyltransferase, acts as a negative modulator of microtubule stability through acetylation of EB1, a protein that controls the plus ends of microtubules. PCAF ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia P,Wang Z,Liu X,Wu B,Wang J,Ward T,Zhang L,Ding X,Gibbons G,Shi Y,Yao X

更新日期：2012-10-09 00:00:00

abstract：:In the mitochondrial genome of the hemichordate Balanoglossus carnosus, the codon AAA, which is assigned to lysine in most metazoans but to asparagine in echinoderms, is absent. Furthermore, the lysine tRNA gene carries an anticodon substitution that renders its gene product unable to decode AAA codons, whereas the as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castresana J,Feldmaier-Fuchs G,Pääbo S

更新日期：1998-03-31 00:00:00

abstract：:Amphibian eggs and embryos as well as mammalian cells have been reported to contain an activity that unwinds double-stranded RNA. We have now found that adenosine residues have been modified in the RNA products of this unwinding activity. Although the modified RNA remains double-stranded, the modification causes the R...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wagner RW,Smith JE,Cooperman BS,Nishikura K

更新日期：1989-04-01 00:00:00

abstract：:The design principles of spider dragline silk, nature's high-performance fiber, are still largely unknown, in particular for the noncrystalline glycine-rich domains, which form the bulk of the material. Here we apply two-dimensional solid-state NMR to determine the distribution of the backbone torsion angles (phi,psi)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Beek JD,Hess S,Vollrath F,Meier BH

更新日期：2002-08-06 00:00:00

abstract：:Defining the processes of autoimmune attack of tissues is important for inhibiting continued tissue destruction. In type 1 diabetes, it is not known how cytotoxic effector T cell responses evolve over time in the pancreatic islets targeted for destruction. We used two-photon microscopy of live, intact, individual isle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedman RS,Lindsay RS,Lilly JK,Nguyen V,Sorensen CM,Jacobelli J,Krummel MF

更新日期：2014-06-24 00:00:00

abstract：:In electron microscopy studies of the endothelial vesicles in frog mesenteric capillaries, an accidental observation was made concerning vesicular organization. When tannic acid was added to already fixed tissue, the mordant reached apparently free vesicles in the cytoplasm under conditions in which vesicular movement...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bundgaard M,Frøkjaer-Jensen J,Crone C

更新日期：1979-12-01 00:00:00