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The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane.

Abstract：

:Bacterial virulence factors are typically surface-associated or secreted molecules that in Gram-negative bacteria must cross the outer membrane (OM). Protein translocation across the bacterial OM is not well understood. To elucidate this process we studied P pilus biogenesis in Escherichia coli. We present high-resolution electron micrographs of the OM usher PapC and show that it forms an oligomeric complex containing a channel approximately 2 nm in diameter. This is large enough to accommodate pilus subunits or the linear tip fibrillum of the pilus but not large enough to accommodate the final 6.8-nm-wide helical pilus rod. We show that P pilus rods can be unraveled into linear fibers by incubation in 50% glycerol. Thus, they are likely to pass through the usher in this unwound form. Packaging of these fibers into their final helical structure would only occur outside the cell, a process that may drive outward growth of the pilus organelles. The usher complex appears to be similar to complexes formed by members of the PulD/pIV family of OM proteins, and thus these two protein families, previously thought to be unrelated, may share structural and functional homologies.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Thanassi DG,Saulino ET,Lombardo MJ,Roth R,Heuser J,Hultgren SJ

doi

10.1073/pnas.95.6.3146

subject

Has Abstract

pub_date

1998-03-17 00:00:00

pages

3146-51

issue

6

eissn

0027-8424

issn

1091-6490

journal_volume

95

pub_type

杂志文章

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