Two-dimensional infrared spectra reveal relaxation of the nonnucleoside inhibitor TMC278 complexed with HIV-1 reverse transcriptase.

abstract：:HUMAN LIVER ALCOHOL DEHYDROGENASE (ALCOHOL: NAD(+) oxidoreductase, EC 1.1.1.1), homogeneous by physicochemical criteria, has been available in quantity only recently [Lange, L. G. & Vallee, B. L. (1976) Biochemistry 15, 4681-4686]. Until now, the biochemical basis of human alcohol metabolism had to be extrapolated fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li TK,Bosron WF,Dafeldecker WP,Lange LG,Vallee BL

更新日期：1977-10-01 00:00:00

abstract：:Exposure of human monocyte-macrophages to as little as 50 microliters of cultured medium from lymphocytes stimulated by concanavalin A (Con A) resulted in a dramatic decrease in the activities of the low density lipoprotein (LDL) receptor pathway, the LDL-dextran sulfate pathway, and the scavenger receptor pathway. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fogelman AM,Seager J,Haberland ME,Hokom M,Tanaka R,Edwards PA

更新日期：1982-02-01 00:00:00

abstract：:Zinniol [1,2-bis(hydroxymethyl)-3-methoxy-4-methyl-5-(3-methyl-2-butenyloxy)benzene], a toxin produced by fungi of the Alternaria group, causes symptoms in plants that resemble those induced by the fungi. The phytotoxin binds to carrot protoplasts and isolated membranes in a saturable and reversible manner. Receptor o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thuleau P,Graziana A,Rossignol M,Kauss H,Auriol P,Ranjeva R

更新日期：1988-08-01 00:00:00

abstract：:Two molecular forms of pyrophosphate-dependent phosphofructokinase (PP(i)-PFK; pyrophosphate:D-fructose-6-phosphate 1-phosphotransferase; EC 2.7.1.90) have been found whose activity depends upon association and dissociation characteristics regulated by fructose 2,6-bisphosphate (Fru-2,6-P(2)). PP(i)-PFK was purified 2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu MX,Smyth DA,Black CC

更新日期：1984-08-01 00:00:00

abstract：:We use stable isotope ratios in feces of wild mountain gorillas (Gorilla beringei) to test the hypothesis that diet shifts within a single year, as measured by dry mass intake, can be recovered. Isotopic separation of staple foods indicates that intraannual changes in the isotopic composition of feces reflect shifts i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blumenthal SA,Chritz KL,Rothman JM,Cerling TE

更新日期：2012-12-26 00:00:00

abstract：:Large-scale biogeographical shifts in vegetation are predicted in response to the altered precipitation and temperature regimes associated with global climate change. Vegetation shifts have profound ecological impacts and are an important climate-ecosystem feedback through their alteration of carbon, water, and energy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adams HD,Guardiola-Claramonte M,Barron-Gafford GA,Villegas JC,Breshears DD,Zou CB,Troch PA,Huxman TE

更新日期：2009-04-28 00:00:00

abstract：:Little is known about how members of the indigenous microflora interact with their mammalian hosts to establish mutually beneficial relationships. We have used a gnotobiotic mouse model to show that Bacteroides thetaiotaomicron, a component of the intestinal microflora of mice and humans, uses a repressor, FucR, as a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hooper LV,Xu J,Falk PG,Midtvedt T,Gordon JI

更新日期：1999-08-17 00:00:00

abstract：:Hybridization in plants leads to transinteractions between the parental genomes and epigenomes that can result in changes to both 24 nt siRNA and cytosine methylation ((m)C) levels in the hybrid. In Arabidopsis the principle processes altering the hybrid methylome are Trans Chromosomal Methylation (TCM) and Trans Chro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greaves IK,Groszmann M,Wang A,Peacock WJ,Dennis ES

更新日期：2014-02-04 00:00:00

abstract：:The localization of endothelin 1 mRNA and endothelin-like immunoreactivity was investigated in samples of neurologically normal nervous system tissue from 10 adults by using in situ hybridization and immunocytochemistry. Tissue sections of spinal cord and dorsal root ganglia were hybridized with an 35S-radiolabeled en...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giaid A,Gibson SJ,Ibrahim BN,Legon S,Bloom SR,Yanagisawa M,Masaki T,Varndell IM,Polak JM

更新日期：1989-10-01 00:00:00

abstract：:Fatty acylation of cysteine residues provides spatial and temporal control of protein function in cells and regulates important biological pathways in eukaryotes. Although recent methods have improved the detection and proteomic analysis of cysteine fatty (S-fatty) acylated proteins, understanding how specific sites a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Percher A,Ramakrishnan S,Thinon E,Yuan X,Yount JS,Hang HC

更新日期：2016-04-19 00:00:00

abstract：:Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： dos Santos Rolo T,Ershov A,van de Kamp T,Baumbach T

更新日期：2014-03-18 00:00:00

abstract：:Functional MRI based on blood oxygenation level-dependent (BOLD) contrast is well established as a neuroimaging technique for detecting neural activity in the cortex of the human brain. While detection and characterization of BOLD signals, as well as their electrophysiological and hemodynamic/metabolic origins, have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ding Z,Huang Y,Bailey SK,Gao Y,Cutting LE,Rogers BP,Newton AT,Gore JC

更新日期：2018-01-16 00:00:00

abstract：:Memory CD8 T cells provide durable protection against diverse intracellular pathogens and can be broadly segregated into distinct circulating and tissue-resident populations. Paradigmatic studies have demonstrated that circulating memory cells can be further divided into effector memory (Tem) and central memory (Tcm) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milner JJ,Nguyen H,Omilusik K,Reina-Campos M,Tsai M,Toma C,Delpoux A,Boland BS,Hedrick SM,Chang JT,Goldrath AW

更新日期：2020-10-13 00:00:00

abstract：:The domestication of cultivated barley has been used as a model system for studying the origins and early spread of agrarian culture. Our previous results indicated that the Tibetan Plateau and its vicinity is one of the centers of domestication of cultivated barley. Here we reveal multiple origins of domesticated bar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai F,Chen ZH,Wang X,Li Z,Jin G,Wu D,Cai S,Wang N,Wu F,Nevo E,Zhang G

更新日期：2014-09-16 00:00:00

abstract：:The temperature dependence of fatty acid spin label resonance spectra and freeze fracture micrographs of sonicated dipalmitoylphosphatidylcholine vesicles in the absence and presence of concanavalin A demonstrate a strong interaction of concanavalin A with these lipid membranes, which results in fusion of the vesicles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van der Bosch J,McConnell M

更新日期：1975-11-01 00:00:00

abstract：:Failure of embryo implantation accounts for a significant percentage of female infertility. Exquisitely coordinated molecular programs govern the interaction between the competent blastocyst and the receptive uterus. Decidualization, the rapid proliferation and differentiation of endometrial stromal cells into decidua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haller M,Yin Y,Ma L

更新日期：2019-09-24 00:00:00

abstract：:Photolyzed rhodopsin catalyzes the exchange of GTP for FDP bound to a protein in retinal rod outer segments. We previously proposed that the GTP complex of this protein regulates the cyclic GMP phosphodiesterase and that it may be the first amplified intermediate in visual excitation [Fung, B. K.-K. & Stryer, L. (1980...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fung BK,Hurley JB,Stryer L

更新日期：1981-01-01 00:00:00

abstract：:A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48). One positive clone was isolated and the nucleotide sequence was determined. It contained 1305 bas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cool DE,Tonks NK,Charbonneau H,Walsh KA,Fischer EH,Krebs EG

更新日期：1989-07-01 00:00:00

abstract：:Brood parasitism as an alternative female breeding tactic is particularly common in ducks, where hosts often receive eggs laid by parasitic females of the same species and raise their offspring. Herein, we test several aspects of a kin selection explanation for this phenomenon in goldeneye ducks (Bucephala clangula) b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andersson M,Ahlund M

更新日期：2000-11-21 00:00:00

abstract：:Tuberculosis (TB) is a major public health problem. One-third of the world's population is estimated to be infected with Mycobacterium tuberculosis (MTB), the etiological agent causing TB, and active disease kills nearly 2 million individuals worldwide every year. Several lines of evidence indicate that interindividua...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barreiro LB,Tailleux L,Pai AA,Gicquel B,Marioni JC,Gilad Y

更新日期：2012-01-24 00:00:00

abstract：:Population expansion in space, or range expansion, is widespread in nature and in clinical settings. Space competition among heterogeneous subpopulations during range expansion is essential to population ecology, and it may involve the interplay of multiple factors, primarily growth and motility of individuals. Struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuo W,Wu Y

更新日期：2020-03-03 00:00:00

abstract：:The alphavbeta3 integrins are linked to human bleeding disorders, and pathogenic hantaviruses regulate the function of alphavbeta3 integrins and cause acute vascular diseases. alphavbeta3 integrins are present in either extended (active) or dramatically bent (inactive) structures, and interconversion of alphavbeta3 co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raymond T,Gorbunova E,Gavrilovskaya IN,Mackow ER

更新日期：2005-01-25 00:00:00

abstract：:Cancer therapies using genes and other macromolecules might realize their full clinical potential if they could be delivered to tumor tissue in optimal quantities. Unfortunately, the compromised circulation within tumors poses a formidable resistance to adequate and uniform penetration of these agents. Previously, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Netti PA,Hamberg LM,Babich JW,Kierstead D,Graham W,Hunter GJ,Wolf GL,Fischman A,Boucher Y,Jain RK

更新日期：1999-03-16 00:00:00

abstract：:Transcription, splicing, and translation are potentially coordinately regulatable in a temporospatial-dependent manner, although supporting experimental evidence for this notion is scarce. Yeast two-hybrid screening of a mammary gland cDNA library with human p21-activated kinase 1 (Pak1) as bait identified polyC-RNA-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng Q,Rayala SK,Gururaj AE,Talukder AH,O'Malley BW,Kumar R

更新日期：2007-04-03 00:00:00

abstract：:The human papillomavirus E7 protein is phosphorylated at the two serines in positions 31/32, which are part of a consensus sequence for casein kinase II (CKII). In this study, we have investigated the effect of CKII phosphorylation site mutations, all of which lead to unphosphorylated E7 proteins. The replacement of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Firzlaff JM,Lüscher B,Eisenman RN

更新日期：1991-06-15 00:00:00

abstract：:Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we reimagine this wavelength division multiplexing (WDM) paradigm in a novel context--the differentiated detection and i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozcelik D,Parks JW,Wall TA,Stott MA,Cai H,Parks JW,Hawkins AR,Schmidt H

更新日期：2015-10-20 00:00:00

abstract：:Pax-6 in vertebrates and its homolog eyeless in Drosophila are known to be essential for eye development. Here we investigate the role of Pax-6 in eye development in another major systematic group, molluscs. We demonstrate that alternatively spliced RNAs derived from a single Pax-6 gene in the squid (Loligo opalescens...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tomarev SI,Callaerts P,Kos L,Zinovieva R,Halder G,Gehring W,Piatigorsky J

更新日期：1997-03-18 00:00:00

abstract：:Despite the common use of the blooming metaphor, its floral inspiration remains poorly understood. Here we study the physical process of blooming in the asiatic lily Lilium casablanca. Our observations show that the edges of the petals wrinkle as the flower opens, suggesting that differential growth drives the deploym...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang H,Mahadevan L

更新日期：2011-04-05 00:00:00

abstract：:In this paper, we report an example of the engineered expression of tetrameric β-galactosidase (β-gal) containing varying numbers of active monomers. Specifically, by combining wild-type and single-nucleotide polymorphism plasmids at varying ratios, tetrameric β-gal was expressed in vitro with one to four active monom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Jiang Y,Chong S,Walt DR

更新日期：2018-08-14 00:00:00

abstract：:Genomic clones of the human non-N-methyl-D-aspartate (non-NMDA) glutamate receptor subunit GluR5 were isolated by high-stringency screening of a cosmid library using the rat cDNA as a probe. The chromosomal localization of the human GluR5 gene has been established. Southern hybridization of DNA isolated from mapping p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eubanks JH,Puranam RS,Kleckner NW,Bettler B,Heinemann SF,McNamara JO

更新日期：1993-01-01 00:00:00