In vivo X-ray cine-tomography for tracking morphological dynamics.

abstract：:Within the Ig superfamily (IgSF), intercellular adhesion molecules (ICAMs) form a subfamily that binds the leukocyte integrin alphaLbeta2. We report a 1.65-A-resolution crystal structure of the ICAM-3 N-terminal domain (D1) in complex with the inserted domain, the ligand-binding domain of alphaLbeta2. This high-resolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song G,Yang Y,Liu JH,Casasnovas JM,Shimaoka M,Springer TA,Wang JH

更新日期：2005-03-01 00:00:00

abstract：:Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lazarides E,Weber K

更新日期：1974-06-01 00:00:00

abstract：:The formation of social attachments is a critical component of human relationships. Infants begin to bond to their caregivers from the moment of birth, and these social bonds continue to provide regulatory emotional functions throughout adulthood. It is difficult to examine the interactions between social experience a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wismer Fries AB,Ziegler TE,Kurian JR,Jacoris S,Pollak SD

更新日期：2005-11-22 00:00:00

abstract：:To study the role of the interferon- (IFN) gammaR2 chain in IFN-gamma signaling and immune function, IFN-gammaR2-deficient mice have been generated and characterized. Cells derived from IFN-gammaR2 -/- mice are unable to activate either JAK/STAT signaling proteins or gene transcription in response to IFN-gamma. The la...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu B,Ebensperger C,Dembic Z,Wang Y,Kvatyuk M,Lu T,Coffman RL,Pestka S,Rothman PB

更新日期：1998-07-07 00:00:00

abstract：:An unbiased survey has been made of the stable, most abundant multi-protein complexes in Desulfovibrio vulgaris Hildenborough (DvH) that are larger than Mr approximately 400 k. The quaternary structures for 8 of the 16 complexes purified during this work were determined by single-particle reconstruction of negatively ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han BG,Dong M,Liu H,Camp L,Geller J,Singer M,Hazen TC,Choi M,Witkowska HE,Ball DA,Typke D,Downing KH,Shatsky M,Brenner SE,Chandonia JM,Biggin MD,Glaeser RM

更新日期：2009-09-29 00:00:00

abstract：:Two groups of mutant clones were isolated from YI adrenocortical tumor cells. One group, Y1(Kin), exhibited altered cytosolic cyclic AMP-dependent protein kinase activity; the second group, Y1(Cyc), exhibited diminished corticotropin-responsive adenylate cyclase activity. Steroidogenic responses to corticotropin and c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rae PA,Gutmann NS,Tsao J,Schimmer BP

更新日期：1979-04-01 00:00:00

abstract：:Disruption of neurotransmitter vesicle dynamics (transport, capacity, release) has been implicated in a variety of neurodegenerative and neuropsychiatric conditions. Here, we report a novel mouse model of enhanced vesicular function via bacterial artificial chromosome (BAC)-mediated overexpression of the vesicular mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohr KM,Bernstein AI,Stout KA,Dunn AR,Lazo CR,Alter SP,Wang M,Li Y,Fan X,Hess EJ,Yi H,Vecchio LM,Goldstein DS,Guillot TS,Salahpour A,Miller GW

更新日期：2014-07-08 00:00:00

abstract：:In Epstein-Barr virus (EBV)-positive Burkitt lymphoma (BL) the role of EBV in the translocation and deregulation of the MYC oncogene remains unknown. By utilizing an EBV-negative BL (BJAB) and several EBV-positive sublines derived from it by in vitro infection, it was possible to show that the presence of the virus wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lacy J,Summers WP,Watson M,Glazer PM,Summers WC

更新日期：1987-08-01 00:00:00

abstract：:A detailed experimental investigation of Fe1+y Te (y = 0.11, 0.12) using pulsed magnetic fields up to 60 T confirms remarkable magnetic shape-memory (MSM) effects. These effects result from magnetoelastic transformation processes in the low-temperature antiferromagnetic state of these materials. The observation of mod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rößler S,Koz C,Wang Z,Skourski Y,Doerr M,Kasinathan D,Rosner H,Schmidt M,Schwarz U,Rößler UK,Wirth S

更新日期：2019-08-20 00:00:00

abstract：:In signal-recognition particle (SRP)-dependent protein targeting to the bacterial plasma membrane, two GTPases, Ffh (a subunit of the bacterial SRP) and FtsY (the bacterial SRP receptor), act as GTPase activating proteins for one another. The molecular mechanism of this reciprocal GTPase activation is poorly understoo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shan SO,Walter P

更新日期：2003-04-15 00:00:00

abstract：:The calcium-activated K(+) channel KCa3.1 plays an important role in T lymphocyte Ca(2+) signaling by helping to maintain a negative membrane potential, which provides an electrochemical gradient to drive Ca(2+) influx. To assess the role of KCa3.1 channels in lymphocyte activation in vivo, we studied T cell function ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di L,Srivastava S,Zhdanova O,Ding Y,Li Z,Wulff H,Lafaille M,Skolnik EY

更新日期：2010-01-26 00:00:00

abstract：:Measurements of the effect of DNA on rates of bimolecular energy transfer between ions provide a direct indication of how cations cluster in regions near DNA and how anions are repelled from the same regions. Energy transfer from luminescent lanthanide ions (in the "rapid-diffusion" limit) probes collision frequencies...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wensel TG,Meares CF,Vlachy V,Matthew JB

更新日期：1986-05-01 00:00:00

abstract：:New neurons are continuously generated in restricted regions of the adult mammalian brain. Although these adult-born neurons have been shown to receive synaptic inputs, little is known about their synaptic outputs. Using retrovirus-mediated birth-dating and labeling in combination with serial section electron microsco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Faulkner RL,Jang MH,Liu XB,Duan X,Sailor KA,Kim JY,Ge S,Jones EG,Ming GL,Song H,Cheng HJ

更新日期：2008-09-16 00:00:00

abstract：:The free radicals nitric oxide and superoxide anion react to form peroxynitrite (ONOO-), a highly toxic oxidant species. In vivo formation of ONOO- has been demonstrated in shock and inflammation. Herein we provide evidence that cytotoxicity in cells exposed to ONOO- is mediated by DNA strand breakage and the subseque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szabó C,Zingarelli B,O'Connor M,Salzman AL

更新日期：1996-03-05 00:00:00

abstract：:Extensive studies have detailed the molecular regulation of individual components of the hemostatic system, including platelets, coagulation factors, and regulatory proteins. Questions remain, however, about how these elements are integrated at the systems level within a rapidly changing physical environment. To answe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tomaiuolo M,Matzko CN,Poventud-Fuentes I,Weisel JW,Brass LF,Stalker TJ

更新日期：2019-02-05 00:00:00

abstract：:Acanthamoeba castellanii contains two enzymes, myosins IA and IB, that exhibit the catalytic properties of a myosin but possess very unusual physical properties, the most striking of which are their single, low molecular weight heavy chain, their globular shape, and their inability to form bipolar filaments. We have n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hammer JA 3rd,Jung G,Korn ED

更新日期：1986-07-01 00:00:00

abstract：:Cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, EC 1.9.3.1) has been resolved into a pair of complexes of unequal molecular weight. The larger complex (electron transfer complex) contains exclusively the oxidation-reduction proteins characteristic of cytochrome oxidase; the smaller complex (ion transfe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fry M,Vande Zande H,Green DE

更新日期：1978-12-01 00:00:00

abstract：:For over 40 y the dominant theory of stomatal behavior has been that plants should open stomates until the carbon gained by an infinitesimal additional opening balances the additional water lost times a water price that is constant at least over short periods. This theory has persisted because of its remarkable succes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolf A,Anderegg WR,Pacala SW

更新日期：2016-11-15 00:00:00

abstract：:Genome-wide association studies found that increased risk for atrial fibrillation (AF), the most common human heart arrhythmia, is associated with noncoding sequence variants located in proximity to PITX2 Cardiomyocyte-specific epigenomic and comparative genomics uncovered 2 AF-associated enhancers neighboring PITX2 w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang M,Hill MC,Kadow ZA,Suh JH,Tucker NR,Hall AW,Tran TT,Swinton PS,Leach JP,Margulies KB,Ellinor PT,Li N,Martin JF

更新日期：2019-11-05 00:00:00

abstract：:Hexamethylene bisacetamide (HMBA) induces in vitro the cytodifferentiation of PCC3/A/1 mouse embryonal carcinoma (EC) cells. In EC cells, actin is associated with surface structures but microfilament bundles are not seen. After 2 days of HMBA treatment, rounded EC cells are converted to flat adhesive ones with a devel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paulin D,Perreau J,Jakob H,Jacob F,Yaniv M

更新日期：1979-04-01 00:00:00

abstract：:The primary structure of proteins S1, the largest protein component of the Escherichia coli ribosome, has been elucidated by determining the amino acid sequence of the protein (from E. coli MRE600) and the nucleotide sequence of the S1 gene (rpsA, of a K-12 strain). The two methods gave results in perfect agreement ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schnier J,Kimura M,Foulaki K,Subramanian AR,Isono K,Wittmann-Liebold B

更新日期：1982-02-01 00:00:00

abstract：:We describe and test a Markov chain model of microsatellite evolution that can explain the different distributions of microsatellite lengths across different organisms and repeat motifs. Two key features of this model are the dependence of mutation rates on microsatellite length and a mutation process that includes bo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kruglyak S,Durrett RT,Schug MD,Aquadro CF

更新日期：1998-09-01 00:00:00

abstract：:d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roper DI,Huyton T,Vagin A,Dodson G

更新日期：2000-08-01 00:00:00

abstract：:We previously demonstrated that platelets express high levels of the tyrosine protein kinase pp60c-src. By a quantitative immunoblot assay, it is shown in this report that pp60c-src represents 0.2-0.4% of total platelet protein. The expression of high levels of pp60c-src in platelets correlated with high levels of tot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golden A,Brugge JS

更新日期：1989-02-01 00:00:00

abstract：:Maintaining a fleet of buses to transport students to school is a major expense for school districts. To reduce costs by reusing buses between schools, many districts spread start times across the morning. However, assigning each school a time involves estimating the impact on transportation costs and reconciling addi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bertsimas D,Delarue A,Martin S

更新日期：2019-03-26 00:00:00

abstract：:Polycomb group genes (PcGs) have been implicated in cancer based on altered levels of expression observed in certain tumors and the behavior of cultured cells containing inserted PcG transgenes. Endometrial stromal tumors provide evidence for a direct causal relationship because they contain several chromosomal transl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li H,Ma X,Wang J,Koontz J,Nucci M,Sklar J

更新日期：2007-12-11 00:00:00

abstract：:A flexible finite difference method is described that gives approximate solutions of linear elliptic partial differential equations, Lu = G, subject to general linear boundary conditions. The method gives high-order accuracy. The values of the unknown approximation function U are determined at mesh points by solving a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lynch RE,Rice JR

更新日期：1978-06-01 00:00:00

abstract：:Given the widely noted increase in the warming effects of rising greenhouse gas concentrations, it has been unclear why global surface temperatures did not rise between 1998 and 2008. We find that this hiatus in warming coincides with a period of little increase in the sum of anthropogenic and natural forcings. Declin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaufmann RK,Kauppi H,Mann ML,Stock JH

更新日期：2011-07-19 00:00:00

abstract：:By incorporating a population number regulating mechanism into the formulation of genic selection involving a pair of alleles (A1 and A2) with respective frequencies x and I-x, it is shown that the change of x in one generation is given by deltax = sx(1-x)/W, in which W is the mean absolute selective value (in Wright'...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kimura M

更新日期：1978-04-01 00:00:00

abstract：:Bitemporal injections of puromycin that primarily affect the hippocampal-entorhinal area induce amnesia of aversive maze-learning in mice for 3 days after training but are ineffective 6 or more days after training. At these later times, additional puromycin sites covering widespread forebrain areas are necessary to in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flexner JB,Flexner LB,Church AC,Rainbow TC,Brunswick DJ

更新日期：1985-11-01 00:00:00