An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin alphaLbeta2.

abstract：:Outer membrane protein (OMP) biogenesis is critical to bacterial physiology because the cellular envelope is vital to bacterial pathogenesis and antibiotic resistance. The process of OMP biogenesis has been studied in vivo, and each of its components has been studied in isolation in vitro. This work integrates paramet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Costello SM,Plummer AM,Fleming PJ,Fleming KG

更新日期：2016-08-16 00:00:00

abstract：:Distinct subsets of thymic epithelial cells (TECs) support T-cell development and selection. Isolated TECs contain multicellular complexes that enclose many viable thymocytes. However, the functions of those TECs, termed thymic nurse cells (TNCs), are unclear and the idea that TNCs are present in vivo is questioned. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakagawa Y,Ohigashi I,Nitta T,Sakata M,Tanaka K,Murata S,Kanagawa O,Takahama Y

更新日期：2012-12-11 00:00:00

abstract：:We have examined the effects of a stromal cell-derived cytokine designated interleukin 11 (IL-11) on the proliferation of murine hemopoietic progenitors in methylcellulose culture. COS cell-conditioned medium containing IL-11 supported formation of granulocyte/macrophage colonies and a small number of multilineage col...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musashi M,Yang YC,Paul SR,Clark SC,Sudo T,Ogawa M

更新日期：1991-02-01 00:00:00

abstract：:The impossibility theorems that abound in the theory of social choice show that there can be no satisfactory method for electing and ranking in the context of the traditional, 700-year-old model. A more realistic model, whose antecedents may be traced to Laplace and Galton, leads to a new theory that avoids all imposs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balinski M,Laraki R

更新日期：2007-05-22 00:00:00

abstract：:A 1-hr exposure of bovine lenses in organ culture to H2O2 concentrations in the range found in the aqueous fluid of patients with cataracts inhibits 86Rb+ influx. At 1 mM H2O2, complete inhibition was observed and further investigated. Membrane permeability is slightly decreased. Although lactate concentrations increa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garner WH,Garner MH,Spector A

更新日期：1983-04-01 00:00:00

abstract：:The protein complex formed by the Ca2+ sensor neuronal calcium sensor 1 (NCS-1) and the guanine exchange factor protein Ric8a coregulates synapse number and probability of neurotransmitter release, emerging as a potential therapeutic target for diseases affecting synapses, such as fragile X syndrome (FXS), the most co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mansilla A,Chaves-Sanjuan A,Campillo NE,Semelidou O,Martínez-González L,Infantes L,González-Rubio JM,Gil C,Conde S,Skoulakis EM,Ferrús A,Martínez A,Sánchez-Barrena MJ

更新日期：2017-02-07 00:00:00

abstract：:We report the application of quantitative mass spectrometry to identify plasma membrane proteins differentially expressed in melanoma cells with high vs. low metastatic abilities. Using stable isotope labeling with amino acids in culture (SILAC) coupled with nanospray tandem mass spectrometry, we identified CUB-domain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu H,Ong SE,Badu-Nkansah K,Schindler J,White FM,Hynes RO

更新日期：2011-01-25 00:00:00

abstract：:alpha-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors undergo constitutive and ligand-induced internalization that requires dynamin and the clathrin adaptor complex AP-2. We report here that an atypical basic motif within the cytoplasmic tails of AMPA-type glutamate receptors directl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kastning K,Kukhtina V,Kittler JT,Chen G,Pechstein A,Enders S,Lee SH,Sheng M,Yan Z,Haucke V

更新日期：2007-02-20 00:00:00

abstract：:Wine chemical compositions, which result from a complex interplay between environmental factors, genetic factors, and viticultural practices, have mostly been studied using targeted analyses of selected families of metabolites. Detailed studies have particularly concerned volatile and polyphenolic compounds because of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gougeon RD,Lucio M,Frommberger M,Peyron D,Chassagne D,Alexandre H,Feuillat F,Voilley A,Cayot P,Gebefügi I,Hertkorn N,Schmitt-Kopplin P

更新日期：2009-06-09 00:00:00

abstract：:Topical application of phorbol myristate acetate (PMA) elicits intense local inflammation that facilitates outgrowth of premalignant lesions in skin after carcinogen exposure. The inflammatory response to PMA treatment activates immune stimulatory mechanisms. However, we show here that PMA exposure also induces plasma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muller AJ,Sharma MD,Chandler PR,Duhadaway JB,Everhart ME,Johnson BA 3rd,Kahler DJ,Pihkala J,Soler AP,Munn DH,Prendergast GC,Mellor AL

更新日期：2008-11-04 00:00:00

abstract：:This paper is the second of three describing a two-fluid theory of binary liquid mixtures. The general theory presented in the preceding paper is used to derive a model for calculating thermodynamic properties of hard-sphere mixtures. Calculations indicate that desirable boundary conditions are satisfied. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brandani V,Prausnitz JM

更新日期：1982-08-01 00:00:00

abstract：:The Cdc6 protein is essential for the assembly of pre-replicative complexes (pre-RCs) at origins of DNA replication in the budding yeast Saccharomyces cerevisiae. This reaction is blocked in vivo by the cyclin-dependent kinase Cdc28p, together with its regulatory subunits, the B type cyclins that are present throughou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donovan S,Harwood J,Drury LS,Diffley JF

更新日期：1997-05-27 00:00:00

abstract：:Two photochromic activators of the electrogenic membrane of the electroplax of Electrophorus electricus are described. Trans-3,3'-bis[alpha-(trimethylammonium)methyl]azobenzene dibromide (Bis-Q), one of the most potent ever reported, is active at concentrations of less than 10(-7) M. Its cis isomer, which is obtained ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartels E,Wassermann NH,Erlanger BF

更新日期：1971-08-01 00:00:00

abstract：:The subventricular zone remains mitotically active throughout life in rodents. Studies with tritiated thymidine, which is incorporated into the DNA of mitotic cells, have revealed that the rodent subventricular zone produces neuroblasts that migrate toward the olfactory bulb along the rostral migratory stream. A simil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bernier PJ,Bedard A,Vinet J,Levesque M,Parent A

更新日期：2002-08-20 00:00:00

abstract：:Emerging and reemerging viruses are responsible for a number of recent epidemic outbreaks. A crucial step in predicting and controlling outbreaks is the timely and accurate characterization of emerging virus strains. We present a portable microfluidic platform containing carbon nanotube arrays with differential filtra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeh YT,Gulino K,Zhang Y,Sabestien A,Chou TW,Zhou B,Lin Z,Albert I,Lu H,Swaminathan V,Ghedin E,Terrones M

更新日期：2020-01-14 00:00:00

abstract：:Proteins potentiated by epidermal growth factor (EGF) to induce a transformed phenotype in non-neoplastic rat kidney fibroblasts in cell culture have been isolated from many non-neoplastic tissues of the adult mouse, including submaxillary gland, kidney, liver, muscle, heart, and brain. They resemble previously descri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts AB,Anzano MA,Lamb LC,Smith JM,Sporn MB

更新日期：1981-09-01 00:00:00

abstract：:The suprachiasmatic nucleus (SCN) is the principal circadian pacemaker of mammals, coordinating daily rhythms of behavior and metabolism. Circadian timekeeping in SCN neurons revolves around transcriptional/posttranslational feedback loops, in which Period (Per) and Cryptochrome (Cry) genes are negatively regulated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maywood ES,Chesham JE,O'Brien JA,Hastings MH

更新日期：2011-08-23 00:00:00

abstract：:Naive CD4 T cells differentiate into several effector lineages, which generate a stronger and more rapid response to previously encountered immunological challenges. Although effector function is a key feature of adaptive immunity, the molecular basis of this process is poorly understood. Here, we investigated the spa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zorca CE,Kim LK,Kim YJ,Krause MR,Zenklusen D,Spilianakis CG,Flavell RA

更新日期：2015-03-31 00:00:00

abstract：:Glioblastoma multiforme (GBM; grade IV astrocytoma) is the most prevalent and aggressive form of primary brain cancer. A subpopulation of multipotent cells termed GBM cancer stem cells (CSCs) play a critical role in tumor initiation, tumor maintenance, metastasis, drug resistance, and recurrence following surgery. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lucki NC,Villa GR,Vergani N,Bollong MJ,Beyer BA,Lee JW,Anglin JL,Spangenberg SH,Chin EN,Sharma A,Johnson K,Sander PN,Gordon P,Skirboll SL,Wurdak H,Schultz PG,Mischel PS,Lairson LL

更新日期：2019-03-26 00:00:00

abstract：:The genes for platelet-derived growth factor (PDGF) A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines. In the present investigation we have studied the expression of these genes in biopsy specimens from human glioblastomas. Hyperplasia of the vascular endothelium is a pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hermansson M,Nistér M,Betsholtz C,Heldin CH,Westermark B,Funa K

更新日期：1988-10-01 00:00:00

abstract：:A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5%...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sipe JD,de Maeyer-Guignard J,Fauconnier B,de Maeyer E

更新日期：1973-04-01 00:00:00

abstract：:Cyclodextrins are cyclic oligosaccharides widely used in the pharmaceutical industry to improve drug delivery and to increase the solubility of hydrophobic compounds. Anabaenolysins are lipopeptides produced by cyanobacteria with potent lytic activity in cholesterol-containing membranes. Here, we identified the 23- to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shishido TK,Jokela J,Kolehmainen CT,Fewer DP,Wahlsten M,Wang H,Rouhiainen L,Rizzi E,De Bellis G,Permi P,Sivonen K

更新日期：2015-11-03 00:00:00

abstract：:Both treatment of disease and basic studies of complex tissues can benefit from directing viral vector infection to specific cell types. We have used a unique cell targeting method to direct viral vector transduction to cerebral cortical neurons expressing the neuregulin (NRG) receptor ErbB4; both NRG and ErbB4 have b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi J,Young JA,Callaway EM

更新日期：2010-09-21 00:00:00

abstract：:Soon after the initiation of sporulation, Bacillus subtilis divides asymmetrically to produce sister cells that have very different developmental fates. Recently, it has been proposed that the differential gene expression which begins soon after this division is due to cell-specific activation of the transcription fac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis PJ,Partridge SR,Errington J

更新日期：1994-04-26 00:00:00

abstract：:Mature larvae of Sarcophaga argyrostoma fail to form puparia when kept in contact with water, but pupariate after having been subsequently exposed to dryness for 30 hr. They become increasingly more sensitive to injected ecdysone the longer the exposure to dry conditions. A second wet treatment halts the production of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zdarek J,Fraenkel G

更新日期：1970-09-01 00:00:00

abstract：:Aromatic polyketides make up a large class of natural products with diverse bioactivity. During biosynthesis, linear poly-β-ketone intermediates are regiospecifically cyclized, yielding molecules with defined cyclization patterns that are crucial for polyketide bioactivity. The aromatase/cyclases (ARO/CYCs) are respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caldara-Festin G,Jackson DR,Barajas JF,Valentic TR,Patel AB,Aguilar S,Nguyen M,Vo M,Khanna A,Sasaki E,Liu HW,Tsai SC

更新日期：2015-12-15 00:00:00

abstract：:The initiator RNAs of mammalian Okazaki fragments are thought to be removed by RNase HI and the 5'-3' flap endonuclease (FEN1). Earlier evidence indicated that the cleavage site of RNase HI is 5' of the last ribonucleotide at the RNA-DNA junction on an Okazaki substrate. In current work, highly purified calf RNase HI ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murante RS,Henricksen LA,Bambara RA

更新日期：1998-03-03 00:00:00

abstract：:In animal cells, exogenous DNA recombines into random chromosomal sites much more frequently than it recombines into homologous sites. Free DNA ends are "recombinogenic" in both processes. To test the effects of specific ends on analogous extrachromosomal processes, we constructed a linear genome of simian virus 40 wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang XB,Wilson JH

更新日期：1987-07-01 00:00:00

abstract：:Progress toward understanding the biology of prostate cancer has been slow due to the few animal research models available to study the spectrum of this uniquely human disease. To develop an animal model for prostate cancer, several lines of transgenic mice were generated by using the prostate-specific rat probasin pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greenberg NM,DeMayo F,Finegold MJ,Medina D,Tilley WD,Aspinall JO,Cunha GR,Donjacour AA,Matusik RJ,Rosen JM

更新日期：1995-04-11 00:00:00

abstract：:As a structure involved in learning and memory, the hippocampus functions as a network. The functional differentiation along the longitudinal axis of the hippocampus is poorly demarcated in comparison with the transverse axis. Using patch clamp recordings in conjunction with post hoc anatomy, we have examined the patt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gloveli T,Dugladze T,Rotstein HG,Traub RD,Monyer H,Heinemann U,Whittington MA,Kopell NJ

更新日期：2005-09-13 00:00:00