当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Photochromic activators of the acetylcholine receptor.

Photochromic activators of the acetylcholine receptor.

Abstract：

:Two photochromic activators of the electrogenic membrane of the electroplax of Electrophorus electricus are described. Trans-3,3'-bis[alpha-(trimethylammonium)methyl]azobenzene dibromide (Bis-Q), one of the most potent ever reported, is active at concentrations of less than 10(-7) M. Its cis isomer, which is obtained from the trans by exposure to light of 330 nm, is practically devoid of activity. Photoregulation of the potential of the membrane takes place in the presence of Bis-Q, presumably because of the conversion of the active trans isomer to the inactive cis isomer in the single-cell electroplax system. The second activator, 3-(alpha-bromomethyl)-3'-[alpha-(trimethylammonium)methyl]azobenzene bromide (QBr) can be covalently attached to the electroplax membrane after reduction of the membrane with dithiothreitol. Activation of the membrane is induced by the covalently linked reagent. Its cis isomer, obtained from the trans by exposure to light of 330 nm, is, like cis-Bis-Q, of very low activity. Both isomers of Bis-Q are equally active as inhibitors of acetylcholinesterase, 50% inhibition occurring at a concentration of 10(-5) M. The possibility of using trans-Bis-Q and trans-QBr to characterize and isolate the receptor protein is discussed.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Bartels E,Wassermann NH,Erlanger BF

doi

10.1073/pnas.68.8.1820

subject

Has Abstract

pub_date

1971-08-01 00:00:00

pages

1820-3

issue

8

eissn

0027-8424

issn

1091-6490

journal_volume

68

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Glycogen synthase kinase 3 inhibitors induce the canonical WNT/β-catenin pathway to suppress growth and self-renewal in embryonal rhabdomyosarcoma.

    abstract：:Embryonal rhabdomyosarcoma (ERMS) is a common pediatric malignancy of muscle, with relapse being the major clinical challenge. Self-renewing tumor-propagating cells (TPCs) drive cancer relapse and are confined to a molecularly definable subset of ERMS cells. To identify drugs that suppress ERMS self-renewal and induce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1317731111

    authors： Chen EY,DeRan MT,Ignatius MS,Grandinetti KB,Clagg R,McCarthy KM,Lobbardi RM,Brockmann J,Keller C,Wu X,Langenau DM

    更新日期：2014-04-08 00:00:00

  • Cytokine-stimulated human immunodeficiency virus replication is inhibited by N-acetyl-L-cysteine.

    abstract：:We show that the stimulation of human immunodeficiency virus (HIV) brought about by tumor necrosis factor alpha and phorbol 12-myristate 13-acetate can be inhibited by adding N-acetyl-L-cysteine (NAC). NAC, which replenishes intracellular glutathione, effectively inhibits the tumor necrosis factor alpha- or phorbol es...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.12.4884

    authors： Roederer M,Staal FJ,Raju PA,Ela SW,Herzenberg LA,Herzenberg LA

    更新日期：1990-06-01 00:00:00

  • A diversity of paracrine signals sustains molecular circadian cycling in suprachiasmatic nucleus circuits.

    abstract：:The suprachiasmatic nucleus (SCN) is the principal circadian pacemaker of mammals, coordinating daily rhythms of behavior and metabolism. Circadian timekeeping in SCN neurons revolves around transcriptional/posttranslational feedback loops, in which Period (Per) and Cryptochrome (Cry) genes are negatively regulated by...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1101767108

    authors： Maywood ES,Chesham JE,O'Brien JA,Hastings MH

    更新日期：2011-08-23 00:00:00

  • Inverse correlation between fatty acid transport protein 4 and vision in Leber congenital amaurosis associated with RPE65 mutation.

    abstract：:Fatty acid transport protein 4 (FATP4), a transmembrane protein in the endoplasmic reticulum (ER), is a recently identified negative regulator of the ER-associated retinal pigment epithelium (RPE)65 isomerase necessary for recycling 11-cis-retinal, the light-sensitive chromophore of both rod and cone opsin visual pigm...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2012623117

    authors： Li S,Gordon WC,Bazan NG,Jin M

    更新日期：2020-12-15 00:00:00

  • Molecular forms of the putative enkephalin precursor BAM-12P in bovine adrenal, pituitary, and hypothalamus.

    abstract：:A highly specific radioimmunoassay for one of the putative adrenomedullary [Met]enkephalin precursors, BAM-12P (Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu-OH), has been developed. The BAM-12P antibodies recognize the COOH-terminal fragment of the peptide from Arg7 to Glu12 and do not crossreact with [Met5]- or [L...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.6.2023

    authors： Baird A,Ling N,Böhlen P,Benoit R,Klepper R,Guillemin R

    更新日期：1982-03-01 00:00:00

  • Desoxyepothilone B is curative against human tumor xenografts that are refractory to paclitaxel.

    abstract：:The epothilones are naturally occurring, cytotoxic macrolides that function through a paclitaxel (Taxol)-like mechanism. Although structurally dissimilar, both classes of molecules lead to the arrest of cell division and eventual cell death by stabilizing cellular microtubule assemblies. The epothilones differ in thei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.26.15798

    authors： Chou TC,Zhang XG,Harris CR,Kuduk SD,Balog A,Savin KA,Bertino JR,Danishefsky SJ

    更新日期：1998-12-22 00:00:00

  • Elimination of potassium channel expression by antisense oligonucleotides in a pituitary cell line.

    abstract：:The clonal rat pituitary cell line GH4C1 expresses the genes for several voltage-dependent potassium channels including Kv1.5 and Kv1.4. Dexamethasone, a glucocorticoid agonist, induces a slowly inactivating potassium current in these cells but does not alter the amplitude of a rapidly inactivating component of potass...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.13.5955

    authors： Chung S,Saal DB,Kaczmarek LK

    更新日期：1995-06-20 00:00:00

  • Three-dimensional structure of the "long" neurotoxin from cobra venom.

    abstract：:The three-dimensional structure of alpha-cobra-toxin, the "long" neurotoxin from the venom of Naja naja siamensis, has been determined at 2.8-A resolution. Crystals grown as hexagonal needles have space group P6522 with unit cell parameters a = b = 74.59 A, c = 42.89 A; one molecule per asymmetric unit. Phases were de...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.5.2400

    authors： Walkinshaw MD,Saenger W,Maelicke A

    更新日期：1980-05-01 00:00:00

  • Transcription factor evolution in eukaryotes and the assembly of the regulatory toolkit in multicellular lineages.

    abstract：:Transcription factors (TFs) are the main players in transcriptional regulation in eukaryotes. However, it remains unclear what role TFs played in the origin of all of the different eukaryotic multicellular lineages. In this paper, we explore how the origin of TF repertoires shaped eukaryotic evolution and, in particul...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1311818110

    authors： de Mendoza A,Sebé-Pedrós A,Šestak MS,Matejcic M,Torruella G,Domazet-Loso T,Ruiz-Trillo I

    更新日期：2013-12-10 00:00:00

  • Brief history of US debt limits before 1939.

    abstract：:Between 1776 and 1920, the US Congress designed more than 200 distinct securities and stated the maximum amount of each that the Treasury could sell. Between 1917 and 1939, Congress gradually delegated all decisions about designing US debt instruments to the Treasury. In 1939, Congress began imposing a limit on the pa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1719687115

    authors： Hall GJ,Sargent TJ

    更新日期：2018-03-20 00:00:00

  • Electrophoretic karyotype of Aspergillus nidulans.

    abstract：:An electrophoretic karyotype of Aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated, with two of the bands migrating as doublets. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.16.6260

    authors： Brody H,Carbon J

    更新日期：1989-08-01 00:00:00

  • Gene discovery in genetically labeled single dopaminergic neurons of the retina.

    abstract：:In the retina, dopamine plays a central role in neural adaptation to light. Progress in the study of dopaminergic amacrine (DA) cells has been limited because they are very few (450 in each mouse retina, 0.005% of retinal neurons). Here, we applied transgenic technology, single-cell global mRNA amplification, and cDNA...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0400913101

    authors： Gustincich S,Contini M,Gariboldi M,Puopolo M,Kadota K,Bono H,LeMieux J,Walsh P,Carninci P,Hayashizaki Y,Okazaki Y,Raviola E

    更新日期：2004-04-06 00:00:00

  • Interspecific gene flow in sympatric oaks.

    abstract：:Variation of chloroplast DNA and nuclear ribosomal DNA (DNA encoding ribosomal RNA) was studied for five species of white oak native to the eastern United States. Although these species differ in many morphological characters and have different (though overlapping) geographical ranges and ecological tolerances, they a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.6.2540

    authors： Whittemore AT,Schaal BA

    更新日期：1991-03-15 00:00:00

  • Gene organization of DC and DX subregions of the human major histocompatibility complex.

    abstract：:The DC and DX subregions of the human major histocompatibility complex (MHC) have been cloned from a cosmid library made from a human B-cell line, Priess. The DC subregion, 48 kilobases, includes the DC alpha and DC beta genes. A second DC-like region, the DX subregion, 35 kilobases, contains the DX alpha gene and a n...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.10.3410

    authors： Okada K,Boss JM,Prentice H,Spies T,Mengler R,Auffray C,Lillie J,Grossberger D,Strominger JL

    更新日期：1985-05-01 00:00:00

  • Inhibition of beta-catenin-mediated transactivation by cadherin derivatives.

    abstract：:We studied the effect of N-cadherin, and its free or membrane-anchored cytoplasmic domain, on the level and localization of beta-catenin and on its ability to induce lymphocyte enhancer-binding factor 1 (LEF-1)-responsive transactivation. These cadherin derivatives formed complexes with beta-catenin and protected it f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.26.15339

    authors： Sadot E,Simcha I,Shtutman M,Ben-Ze'ev A,Geiger B

    更新日期：1998-12-22 00:00:00

  • Origin of the Metazoa.

    abstract：:The origin of the multicellular animals has been investigated by rate invariant analysis of 18S rRNA sequences. These analyses indicate that (i) the Metazoa is a monophyletic taxon; (ii) the Deuterostomia is a monophyletic taxon; (iii) the Annelida-Mollusca lineage is the sister group of an arthropod subgroup; and (iv...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.2.763

    authors： Lake JA

    更新日期：1990-01-01 00:00:00

  • Low Temperature Electronic Absorption Spectra of Oxidized and Reduced Spinach Ferredoxins. Evidence for Nonequivalent Iron(III) Sites.

    abstract：:The electronic absorption spectra of oxidized and reduced spinach ferredoxins have been measured between 1200 and 600 nm at low temperature in D(2)O/ethylene glycol glasses. Relatively weak absorption bands are observed at 720, 820, and 920 nm in oxidized ferredoxin, and at 652, 820, and 920 nm in reduced ferredoxin. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.1.125

    authors： Rawlings J,Siiman O,Gray HB

    更新日期：1974-01-01 00:00:00

  • Lysine methyltransferase G9a methylates the transcription factor MyoD and regulates skeletal muscle differentiation.

    abstract：:Skeletal muscle cells have served as a paradigm for understanding mechanisms leading to cellular differentiation. The proliferation and differentiation of muscle precursor cells require the concerted activity of myogenic regulatory factors including MyoD. In addition, chromatin modifiers mediate dynamic modifications ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1111628109

    authors： Ling BM,Bharathy N,Chung TK,Kok WK,Li S,Tan YH,Rao VK,Gopinadhan S,Sartorelli V,Walsh MJ,Taneja R

    更新日期：2012-01-17 00:00:00

  • Resistance of simian virus 40-transformed hamster cells to the cytolytic effect of activated macrophages: a possible factor in species-specific viral oncogenicity.

    abstract：:Simian virus 40 (SV40)-transformed hamster cells were relatively resistant to the lytic effect of activated macrophages from animals with chronic intracellular infections. Conversely, SV40-transformed mouse and rat cells and adenovirus 2-transformed hamster cells were highly susceptible to destruction by tumoricidal a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.11.6773

    authors： Cook JL,Hibbs JB Jr,Lewis AM Jr

    更新日期：1980-11-01 00:00:00

  • Serotonin receptor-mediated stimulation of bovine smooth muscle cell prostacyclin synthesis and its modulation by platelet-derived growth factor.

    abstract：:Serotonin (5-hydroxytryptamine; 0.5 microM and above) stimulated the synthesis of prostacyclin (as measured by radioimmunoassay of 6-ketoprostaglandin F1 alpha) by bovine aortic smooth muscle cells in culture. This effect was structurally specific; a similar response was not elicited by the other indoles (tryptophan, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.11.7134

    authors： Coughlin SR,Moskowitz MA,Antoniades HN,Levine L

    更新日期：1981-11-01 00:00:00

  • Isolation of cDNA clones specific for collagen IV and laminin from mouse teratocarcinoma cells.

    abstract：:The synthesis of the proteins laminin and collagen IV is stimulated approximately equal to 20-fold in F9 mouse teratocarcinoma stem cells after treatment of the cells with retinoic acid and N6, O2'-dibutyryl-cAMP (Bt2cAMP). A cDNA library from F9 cells treated with retinoic acid, Bt2cAMP, and theophylline (F9-R + DBC ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.19.5880

    authors： Wang SY,Gudas LJ

    更新日期：1983-10-01 00:00:00

  • Yin6, a fission yeast Int6 homolog, complexes with Moe1 and plays a role in chromosome segregation.

    abstract：:The INT6 gene has been implicated in human breast cancer formation, but its function is unknown. We isolated an Int6 homolog from fission yeast, Yin6, by its binding to a conserved protein in the Ras pathway, Moe1. Yin6 and Moe1 converge on the same protein complex to promote microtubule instability/disassembly. Yin6 ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.26.14370

    authors： Yen HC,Chang EC

    更新日期：2000-12-19 00:00:00

  • Enzyme catalysis by entropy without Circe effect.

    abstract：:Entropic effects have often been invoked to explain the extraordinary catalytic power of enzymes. In particular, the hypothesis that enzymes can use part of the substrate-binding free energy to reduce the entropic penalty associated with the subsequent chemical transformation has been very influential. The enzymatic r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1521020113

    authors： Kazemi M,Himo F,Åqvist J

    更新日期：2016-03-01 00:00:00

  • The roles of specific xanthophylls in photoprotection.

    abstract：:Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the green alga Chlamydomonas reinhardtii revealed functions for specific xanthophylls in the nonradiative dissipation of exce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.25.14162

    authors： Niyogi KK,Björkman O,Grossman AR

    更新日期：1997-12-09 00:00:00

  • Cardiac myosin-binding protein C decorates F-actin: implications for cardiac function.

    abstract：:Cardiac myosin-binding protein C (cMyBP-C) is an accessory protein of striated muscle sarcomeres that is vital for maintaining regular heart function. Its 4 N-terminal regulatory domains, C0-C1-m-C2 (C0C2), influence actin and myosin interactions, the basic contractile proteins of muscle. Using neutron contrast variat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0808903105

    authors： Whitten AE,Jeffries CM,Harris SP,Trewhella J

    更新日期：2008-11-25 00:00:00

  • Insufficiently dehydrated hydrogen bonds as determinants of protein interactions.

    abstract：:The prediction of binding sites and the understanding of interfaces associated with protein complexation remains an open problem in molecular biophysics. This work shows that a crucial factor in predicting and rationalizing protein-protein interfaces can be inferred by assessing the extent of intramolecular desolvatio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0136888100

    authors： Fernández A,Scheraga HA

    更新日期：2003-01-07 00:00:00

  • Molecular interactions and inhibition of the staphylococcal biofilm-forming protein SdrC.

    abstract：:Staphylococcus aureus forms biofilms on indwelling medical devices using a variety of cell-surface proteins. There is growing evidence that specific homophilic interactions between these proteins represent an important mechanism of cell accumulation during biofilm formation, but the underlying molecular mechanisms are...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616805114

    authors： Feuillie C,Formosa-Dague C,Hays LM,Vervaeck O,Derclaye S,Brennan MP,Foster TJ,Geoghegan JA,Dufrêne YF

    更新日期：2017-04-04 00:00:00

  • Ezrin-mediated apical integrity is required for intestinal homeostasis.

    abstract：:Individual cell types are defined by architecturally and functionally specialized cortical domains. The Ezrin, Radixin, and Moesin (ERM) proteins play a major role in organizing cortical domains by assembling membrane protein complexes and linking them to the cortical actin cytoskeleton. Many studies have focused on t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1103418108

    authors： Casaletto JB,Saotome I,Curto M,McClatchey AI

    更新日期：2011-07-19 00:00:00

  • Attention deficit/hyperactivity disorder children with a 7-repeat allele of the dopamine receptor D4 gene have extreme behavior but normal performance on critical neuropsychological tests of attention.

    abstract：:An association of the dopamine receptor D4 (DRD4) gene located on chromosome 11p15.5 and attention deficit/hyperactivity disorder (ADHD) has been demonstrated and replicated by multiple investigators. A specific allele [the 7-repeat of a 48-bp variable number of tandem repeats (VNTR) in exon 3] has been proposed as an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.080070897

    authors： Swanson J,Oosterlaan J,Murias M,Schuck S,Flodman P,Spence MA,Wasdell M,Ding Y,Chi HC,Smith M,Mann M,Carlson C,Kennedy JL,Sergeant JA,Leung P,Zhang YP,Sadeh A,Chen C,Whalen CK,Babb KA,Moyzis R,Posner MI

    更新日期：2000-04-25 00:00:00

  • Optical mapping of site-directed cleavages on single DNA molecules by the RecA-assisted restriction endonuclease technique.

    abstract：:Fluorescence in situ hybridization (FISH) resolution has advanced because newer techniques use increasingly decondensed chromatin. FISH cannot analyze restriction enzyme cutting sites due to limitations of the hybridization and detection technologies. The RecA-assisted restriction endonuclease (RARE) technique cleaves...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.1.165

    authors： Wang YK,Huff EJ,Schwartz DC

    更新日期：1995-01-03 00:00:00