Yin6, a fission yeast Int6 homolog, complexes with Moe1 and plays a role in chromosome segregation.

abstract：:Oligodeoxyribonucleotides with site-specific modifications have been used as substrates for Escherichia coli DNA polymerase I holoenzyme and Klenow fragment. Modifications included the bulky guanine-8-aminofluorene adduct and a guanine oxidation product resembling the product of photosensitized DNA oxidation. By a com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Connor D,Stöhrer G

更新日期：1985-04-01 00:00:00

abstract：:E2A helix-loop-helix proteins are involved in the control of various developmental pathways. We show here by in situ hybridization that E2A transcripts are present in most embryonic and adult tissues. However, no E2A expression is detectable in heart and nonproliferative regions of the brain and spinal cord. Highest l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts VJ,Steenbergen R,Murre C

更新日期：1993-08-15 00:00:00

abstract：:Transient Receptor Potential Melastatin-8 (TRPM8), a recently identified member of the transient receptor potential (TRP) family of ion channels, is activated by mild cooling and by chemical compounds such as the supercooling agent, icilin. Since cooling, possibly involving TRPM8 stimulation, diminishes injury-induced...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramachandran R,Hyun E,Zhao L,Lapointe TK,Chapman K,Hirota CL,Ghosh S,McKemy DD,Vergnolle N,Beck PL,Altier C,Hollenberg MD

更新日期：2013-04-30 00:00:00

abstract：:Two functional domains in the primary structure of basic fibroblast growth factor (FGF) have been identified on the basis of their ability to interact with the FGF receptor, bind radiolabeled heparin, and modulate the cellular response to FGF. Peptides derived from these two functional domains can act as partial agoni...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baird A,Schubert D,Ling N,Guillemin R

更新日期：1988-04-01 00:00:00

abstract：:The catalytic power of enzymes containing coenzyme B12 has been, in some respects, the "last bastion" for the strain hypothesis. Our previous study of this system established by a careful sampling that the major part of the catalytic effect is due to the electrostatic interaction between the ribose of the ado group an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schopf P,Mills MJ,Warshel A

更新日期：2015-04-07 00:00:00

abstract：:Although it is accepted that regulatory T cells (T regs) contribute to cancer progression, most studies in the field consider nonantigen-specific suppression. Here, we show the presence of tumor antigen-specific CD4(+) T regs in the blood of patients with metastatic melanoma. These CD4(+) T regs recognize a broad rang...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vence L,Palucka AK,Fay JW,Ito T,Liu YJ,Banchereau J,Ueno H

更新日期：2007-12-26 00:00:00

abstract：:Extracts of Escherichia coli strains infected with bacteriophage phiX174 catalyze DNA synthesis dependent on double-stranded, circular phiX174 replicative form I (phiX RFI) by a semiconservative process. The reaction required Mg++, ATP, all four dNTP, and exogenous phiX RFI DNA as template and yielded phiX RFI and phi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sumida-Yasumoto C,Yudelevich A,Hurwitz J

更新日期：1976-06-01 00:00:00

abstract：:A transformation-defective mutant of Abelson murine leukemia virus (A-MuLV), called A-MuLV-P92td, has been isolated. The mutant encodes a serologically identifiable A-MuLV protein of molecular weight 92,000 (P92) but it lacks the ability to transform either fibroblasts or bone marrow lymphoid cells. In contrast to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Witte ON,Goff S,Rosenberg N,Baltimore D

更新日期：1980-08-01 00:00:00

abstract：:Glc3Man9GlcNAc2 is the preferred substrate of the oligosaccharyltransferase of N-linked glycosylation of proteins, but nonglucosylated oligosaccharides can be transferred to proteins in Saccharomyces cerevisiae. Mutations affecting the addition of the three terminal glucose residues lead to accumulation of Man9GlcNAc2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stagljar I,te Heesen S,Aebi M

更新日期：1994-06-21 00:00:00

abstract：:A mixture of two recombinant plasmids was microinjected into mouse thymidine kinase-negative fibroblasts (L cells). One plasmid contained the thymidine kinase gene of herpes simplex virus type I and the other contained the human beta globin gene. Seven fibroblast colonies arising from injected cells incubated in hypox...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson WF,Killos L,Sanders-Haigh L,Kretschmer PJ,Diacumakos EG

更新日期：1980-09-01 00:00:00

abstract：:A systematic comparison of crystal structures of nine different B-DNA dodecamers, in three different space groups, with and without A-tracts, shows that crystal packing or lattice forces are of secondary importance for helix axis bending, minor-groove width, and propeller twist. While other local helix parameters may ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickerson RE,Goodsell DS,Neidle S

更新日期：1994-04-26 00:00:00

abstract：:α-SNAP [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein] and NSF proteins are conserved across eukaryotes and sustain cellular vesicle trafficking by mediating disassembly and reuse of SNARE protein complexes, which facilitate fusion of vesicles to target membranes. However, certain haplotypes of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bayless AM,Smith JM,Song J,McMinn PH,Teillet A,August BK,Bent AF

更新日期：2016-11-22 00:00:00

abstract：:The ability to modulate the collective properties of correlated electron systems at their interfaces and surfaces underpins the burgeoning field of "designer" quantum materials. Here, we show how an electronic reconstruction driven by surface polarity mediates a Stoner-like magnetic instability to itinerant ferromagne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazzola F,Sunko V,Khim S,Rosner H,Kushwaha P,Clark OJ,Bawden L,Marković I,Kim TK,Hoesch M,Mackenzie AP,King PDC

更新日期：2018-12-18 00:00:00

abstract：:The field of perceptual learning has identified changes in perceptual templates as a powerful mechanism mediating the learning of statistical regularities in our environment. By measuring threshold-vs.-contrast curves using an orientation identification task under varying levels of external noise, the perceptual templ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bejjanki VR,Zhang R,Li R,Pouget A,Green CS,Lu ZL,Bavelier D

更新日期：2014-11-25 00:00:00

abstract：:C factor, an intercellular signaling protein, is required for aggregation and sporulation of the social bacterium, Myxococcus xanthus. We report that C factor, which normally is associated with the cell surface, provides input to the Frz signal transduction cascade. Elements of this cascade have sequence homology to b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Søgaard-Andersen L,Kaiser D

更新日期：1996-04-02 00:00:00

abstract：:A modification of the polymerase chain reaction has been used to establish the fact that a collection of Staphylococcus aureus toxins are "superantigens," each of which interacts with the T-cell alpha beta receptor of human T cells by means of a specific set of V beta elements. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi YW,Kotzin B,Herron L,Callahan J,Marrack P,Kappler J

更新日期：1989-11-01 00:00:00

abstract：:The hetR gene plays a very important role in cell differentiation of heterocystous cyanobacteria. To understand the mechanism of the hetR gene product in regulation of heterocyst differentiation, the recombinant HetR protein (rHetR) was overproduced in Escherichia coli. Purified rHetR was unstable and degraded easily ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou R,Wei X,Jiang N,Li H,Dong Y,Hsi KL,Zhao J

更新日期：1998-04-28 00:00:00

abstract：:A mathematical model is presented that describes the effects of hormone concentration on receptor saturation and biological response in systems dependent upon the generation of a secondary mediator such as cyclic AMP. The analysis makes the following assumptions: (i) the binding of hormone to its receptor is a reversi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strickland S,Loeb JN

更新日期：1981-03-01 00:00:00

abstract：:Nitric oxide (NO), synthesized from L-arginine by NO synthases (NOS), plays an essential role in the regulation of cerebrovascular tone. Adenoviral vectors have been widely used to transfer recombinant genes to different vascular beds. To determine whether the recombinant endothelial NOS (eNOS) gene can be delivered i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen AF,Jiang SW,Crotty TB,Tsutsui M,Smith LA,O'Brien T,Katusic ZS

更新日期：1997-11-11 00:00:00

abstract：:E12 and E47 are two non-tissue-specific helix-loop-helix (HLH) transcription factors encoded by the E2A gene. Previous studies suggested that they are involved in regulation of differentiation in many tissue types including muscle, blood, and nerve through direct heterodimer interactions with tissue-specific HLH prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhuang Y,Kim CG,Bartelmez S,Cheng P,Groudine M,Weintraub H

更新日期：1992-12-15 00:00:00

abstract：:Escherichia coli phage N4 transcription is resistant to rifampicin, an inhibitor of the host RNA polymerase, even when the drug is added prior to infection. A rifampicin-resistant RNA polymerase has been detected in disrupted N4 virions. This enzyme shows a requirement for the four ribonucleoside 5'-triphosphates and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Falco SC,Laan KV,Rothman-Denes LB

更新日期：1977-02-01 00:00:00

abstract：:Metazoan embryogenesis is controlled by a limited number of signaling modules that are used repetitively at successive developmental stages. The development of social amoebas shows similar reiterated use of cAMP-mediated signaling. In the model Dictyostelium discoideum, secreted cAMP acting on 4 cAMP receptors (cARs1-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawabe Y,Morio T,James JL,Prescott AR,Tanaka Y,Schaap P

更新日期：2009-04-28 00:00:00

abstract：:Climate change is expected to influence the capacities of the land and oceans to act as repositories for anthropogenic CO2 and hence provide a feedback to climate change. A series of experiments with the National Center for Atmospheric Research-Climate System Model 1 coupled carbon-climate model shows that carbon sink...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fung IY,Doney SC,Lindsay K,John J

更新日期：2005-08-09 00:00:00

abstract：:Numerous relatively short regions within intrinsically disordered proteins (IDPs) serve as molecular recognition elements (MoREs). They fold into ordered structures upon binding to their partner molecules. Currently, there is still a lack of in-depth understanding of how coupled binding and folding occurs in MoREs. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Chu X,Longhi S,Roche P,Han W,Wang E,Wang J

更新日期：2013-10-01 00:00:00

abstract：:Classic physiology studies dating to the 1930s demonstrate that moderate or transient glucocorticoid (GC) exposure improves muscle performance. The ergogenic properties of GCs are further evidenced by their surreptitious use as doping agents by endurance athletes and poorly understood efficacy in Duchenne muscular dys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrison-Nozik A,Anand P,Zhu H,Duan Q,Sabeh M,Prosdocimo DA,Lemieux ME,Nordsborg N,Russell AP,MacRae CA,Gerber AN,Jain MK,Haldar SM

更新日期：2015-12-08 00:00:00

abstract：:Low-barrier hydrogen bonds (LBHBs) have been proposed to play roles in protein functions, including enzymatic catalysis and proton transfer. Transient formation of LBHBs is expected to stabilize specific reaction intermediates. However, based on experimental results and theoretical considerations, arguments against th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamaguchi S,Kamikubo H,Kurihara K,Kuroki R,Niimura N,Shimizu N,Yamazaki Y,Kataoka M

更新日期：2009-01-13 00:00:00

abstract：:We have investigated the biochemical basis for negative regulation of interleukin 2 receptor alpha-chain (IL-2R alpha) gene expression. Transient transfection studies employing internally deleted forms of the IL-2R alpha promoter localized a negative regulatory element (NRE) between nucleotides -400 and -368 relative ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith MR,Greene WC

更新日期：1989-11-01 00:00:00

abstract：:Although x-ray crystallography is the most widely used method for macromolecular structure determination, it does not provide dynamical information, and either experimental tricks or complementary experiments must be used to overcome the inherently static nature of crystallographic structures. Here we used specific x-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colletier JP,Bourgeois D,Sanson B,Fournier D,Sussman JL,Silman I,Weik M

更新日期：2008-08-19 00:00:00

abstract：:Contact of axons with target territories results in the formation of synapses, specific junctional complexes that may represent a final stage of neuronal maturation. Synaptosomal-associated protein 25 (SNAP-25) is a component of particular nerve terminals recently identified in rodent brain. To evaluate the structure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Catsicas S,Larhammar D,Blomqvist A,Sanna PP,Milner RJ,Wilson MC

更新日期：1991-02-01 00:00:00

abstract：:Limited trypsinization of the fatty acid synthetase multienzyme complex from rat mammary gland results in the release of a protein, molecular weight 32,000, with thioesterase activity. The other components of the multienzyme complex--the acyl carrier protein, acetyl and malonyl transferases, condensing enzyme, keto re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith S,Agradi E,Libertini L,Dileepan KN

更新日期：1976-04-01 00:00:00