Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography.

abstract：:Understanding the mechanisms of nanoparticle interaction with cell membranes is essential for designing materials for applications such as bioimaging and drug delivery, as well as for assessing engineered nanomaterial safety. Much attention has focused on nanoparticles that bind strongly to biological membranes or ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foreman-Ortiz IU,Liang D,Laudadio ED,Calderin JD,Wu M,Keshri P,Zhang X,Schwartz MP,Hamers RJ,Rotello VM,Murphy CJ,Cui Q,Pedersen JA

更新日期：2020-11-10 00:00:00

abstract：:In a microarray-based gene profiling analysis of Müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish, we found that 2 genes required for regeneration of fin and heart tissues in zebrafish, hspd1 (heat shock 60-kDa protein 1) and mps1 (monopolar spindle 1), were up-regulated. Expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin Z,Barthel LK,Raymond PA

更新日期：2009-06-09 00:00:00

abstract：:We examined human immunodeficiency virus (HIV) production in cultured mononuclear cells from 36 seropositive homosexual males. Production was detected by using an HIV p24 antigen ELISA assay in blood mononuclear cells in 54% of asymptomatic, 71% of acquired immunodeficiency syndrome (AIDS)-related complex, and 100% of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McElrath MJ,Pruett JE,Cohn ZA

更新日期：1989-01-01 00:00:00

abstract：:Saccades are rapid eye movements that orient the visual axis toward objects of interest to allow their processing by the central, high-acuity retina. Our ability to collect visual information efficiently relies on saccadic accuracy, which is limited by a combination of uncertainty in the location of the target and mot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lisi M,Solomon JA,Morgan MJ

更新日期：2019-08-06 00:00:00

abstract：:Targeted gene modification mediated by single-stranded oligonucleotides (SSOs) holds great potential for widespread use in a number of biological and biomedical fields, including functional genomics and gene therapy. By using this approach, specific genetic changes have been created in a number of prokaryotic and euka...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu XS,Xin L,Yin WX,Shang XY,Lu L,Watt RM,Cheah KS,Huang JD,Liu DP,Liang CC

更新日期：2005-02-15 00:00:00

abstract：:A number of rare copy number variants (CNVs), including both deletions and duplications, have been associated with developmental disorders, including schizophrenia, autism, intellectual disability, and epilepsy. Pathogenicity may derive from dosage sensitivity of one or more genes contained within the CNV locus. To un...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLysaght A,Makino T,Grayton HM,Tropeano M,Mitchell KJ,Vassos E,Collier DA

更新日期：2014-01-07 00:00:00

abstract：:CPEB, a cytoplasmic polyadenylation element-binding protein, plays an important role in translational control of maternal mRNAs in early animal development. During Xenopus oocyte maturation, CPEB undergoes a Cdc2-mediated phosphorylation- and ubiquitin-dependent degradation that is required for proper entry into meios...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Setoyama D,Yamashita M,Sagata N

更新日期：2007-11-13 00:00:00

abstract：:Dysregulation of cyclin-dependent kinase 5 (cdk5) per relative concentrations of its activators p35 and p25 is implicated in neurodegenerative diseases. P35 has a short t½ and undergoes rapid proteasomal degradation in its membrane-bound myristoylated form. P35 is converted by calpain to p25, which, along with an exte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai SY,Pokrass MJ,Klauer NR,Nohara H,Su TP

更新日期：2015-05-26 00:00:00

abstract：:Mutations responsible for inherited disease may act by disrupting normal transcriptional splicing. Such mutations can be difficult to detect, and their effects difficult to characterize, because many lie deep within exons or introns where they may alter splice enhancers or silencers or introduce new splice acceptors o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Casadei S,Gulsuner S,Shirts BH,Mandell JB,Kortbawi HM,Norquist BS,Swisher EM,Lee MK,Goldberg Y,O'Connor R,Tan Z,Pritchard CC,King MC,Walsh T

更新日期：2019-12-16 00:00:00

abstract：:Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vician L,Lim IK,Ferguson G,Tocco G,Baudry M,Herschman HR

更新日期：1995-03-14 00:00:00

abstract：:Stearoyl-acyl-carrier-protein-desaturase-mediated conversion of stearic acid (18:0) to oleic acid (18:1) is a key step, which regulates levels of unsaturated fatty acids in cells. We previously showed that stearoyl-acyl-carrier-protein-desaturase mutants ssi2/fab2 carrying a loss-of-function mutation in the plastidial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kachroo A,Venugopal SC,Lapchyk L,Falcone D,Hildebrand D,Kachroo P

更新日期：2004-04-06 00:00:00

abstract：:We analyzed gene expression patterns in human gastric cancers by using cDNA microarrays representing approximately equal 30,300 genes. Expression of PLA2G2A, a gene previously implicated as a modifier of the Apc(Min/+) (multiple intestinal neoplasia 1) mutant phenotype in the mouse, was significantly correlated with p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung SY,Chen X,Chu KM,Yuen ST,Mathy J,Ji J,Chan AS,Li R,Law S,Troyanskaya OG,Tu IP,Wong J,So S,Botstein D,Brown PO

更新日期：2002-12-10 00:00:00

abstract：:Clonal deletion provides an important mechanism for the elimination of autoreactive T cells. Deletion is accomplished by programmed cell death directed by interaction of the T-cell receptor (TCR) of the developing thymocyte with major histocompatibility complex elements in the thymic environment. In this report we pre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Russell JH,White CL,Loh DY,Meleedy-Rey P

更新日期：1991-03-15 00:00:00

abstract：:An unusual cAMP signaling system mediates many of the events that prepare spermatozoa to meet the egg. Its components include the atypical, bicarbonate-stimulated, sperm adenylyl cyclase and a cAMP-dependent protein kinase (PKA) with the unique catalytic subunit termed Calpha(2) or C(s). We generated mice that lack Ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nolan MA,Babcock DF,Wennemuth G,Brown W,Burton KA,McKnight GS

更新日期：2004-09-14 00:00:00

abstract：:Herpesvirus entry into cells requires the coordinated action of multiple virus envelope glycoproteins, including gH, gL, and gB. For EBV, the gp42 protein assembles into complexes with gHgL heterodimers and binds HLA class II to activate gB-mediated membrane fusion with B cells. EBV tropism is dictated by gp42 levels ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sathiyamoorthy K,Jiang J,Möhl BS,Chen J,Zhou ZH,Longnecker R,Jardetzky TS

更新日期：2017-10-10 00:00:00

abstract：:We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang AS,Barbas CF,Janda KD,Benkovic SJ,Lerner RA

更新日期：1991-05-15 00:00:00

abstract：:We have characterized seven murine retinoic acid receptor gamma cDNA isoforms (mRAR-gamma 1 to -gamma 7) generated by alternative splicing of at least seven exons. These isoforms differ from one another in their 5' untranslated region and in two cases (mRAR-gamma 1 and -gamma 2) differ in their N-terminal A region, wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kastner P,Krust A,Mendelsohn C,Garnier JM,Zelent A,Leroy P,Staub A,Chambon P

更新日期：1990-04-01 00:00:00

abstract：:Neovascular diseases of the retina include age-related macular degeneration and diabetic retinopathy, and together they comprise the leading causes of adult-onset blindness in developed countries. Current surgical, pharmaceutical, and laser therapies for age-related macular degeneration (AMD) rarely result in improved...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raisler BJ,Berns KI,Grant MB,Beliaev D,Hauswirth WW

更新日期：2002-06-25 00:00:00

abstract：:The brain contains an astonishing diversity of neurons, each expressing only one set of ion channels out of the billions of potential channel combinations. Simple organizing principles are required for us to make sense of this abundance of possibilities and wealth of related data. We suggest that energy minimization s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasenstaub A,Otte S,Callaway E,Sejnowski TJ

更新日期：2010-07-06 00:00:00

abstract：:Dematerialization, represented by declining consumption per GDP of energy or of goods, offers some hope for rising environmental quality with development. The declining proportion of income spent on staples as affluence grows, which income elasticity <1.0 measures, makes dematerialization widespread. Further, as learn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ausubel JH,Waggoner PE

更新日期：2008-09-02 00:00:00

abstract：:Mutations in the gene encoding the transcription factor autoimmune regulator (AIRE) are responsible for autoimmune polyendocrinopathy candidiasis ectodermal dystrophy syndrome. AIRE directs expression of tissue-restricted antigens in the thymic medulla and in lymph node stromal cells and thereby substantially contribu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pomié C,Vicente R,Vuddamalay Y,Lundgren BA,van der Hoek M,Enault G,Kagan J,Fazilleau N,Scott HS,Romagnoli P,van Meerwijk JP

更新日期：2011-07-26 00:00:00

abstract：:Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devise...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aigner-Held R,Campbell RA,Daves GD Jr

更新日期：1979-12-01 00:00:00

abstract：:Conjugated linoleic acids (CLAs) affect body fat gain, carcinogenesis, insulin resistance, and lipid peroxidation in mammals. Several isomers of CLA exist, of which the (9Z, 11E) and (10E, 12Z) isomers have beneficial effects on human metabolism but are scarce in foods. Bacterial polyunsaturated fatty acid isomerases ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liavonchanka A,Hornung E,Feussner I,Rudolph MG

更新日期：2006-02-21 00:00:00

abstract：:The nonlinear nature of integration among cortical brain areas renders the effective connectivity between them inherently dynamic and context-sensitive. One emerging architectural principle of functional brain organization, which rests explicitly on these nonlinear interactions, is that neuronal responses expressed at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friston KJ,Büchel C

更新日期：2000-06-20 00:00:00

abstract：:High-resolution physical maps of the genomes of three Rhodobacter capsulatus strains, derived from ordered cosmid libraries, were aligned. The 1.2-Mb segment of the SB1003 genome studied here is adjacent to a 1-Mb region analyzed previously [Fonstein, M., Nikolskaya, T. & Haselkorn, H. (1995) J. Bacteriol. 177, 2368-2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nikolskaya T,Fonstein M,Haselkorn R

更新日期：1995-11-07 00:00:00

abstract：:The major small nuclear ribonucleoproteins (snRNPs) U1, U2, U4/U6, and U5 share a set of common proteins denoted B/B', D1, D2, D3, E, F, and G which play an important part in the biogenesis of the snRNPs. In addition, there is a link between the common proteins and autoimmunity; the three D proteins, together with B/B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehmeier T,Raker V,Hermann H,Lührmann R

更新日期：1994-12-06 00:00:00

abstract：:Climate warming is expected to mobilize northern permafrost and peat organic carbon (PP-C), yet magnitudes and system specifics of even current releases are poorly constrained. While part of the PP-C will degrade at point of thaw to CO2 and CH4 to directly amplify global warming, another part will enter the fluvial ne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wild B,Andersson A,Bröder L,Vonk J,Hugelius G,McClelland JW,Song W,Raymond PA,Gustafsson Ö

更新日期：2019-05-21 00:00:00

abstract：:The mechanism by which antifreeze proteins (AFPs) irreversibly bind to ice has not yet been resolved. The ice-binding site of an AFP is relatively hydrophobic, but also contains many potential hydrogen bond donors/acceptors. The extent to which hydrogen bonding and the hydrophobic effect contribute to ice binding has ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garnham CP,Campbell RL,Davies PL

更新日期：2011-05-03 00:00:00

abstract：:Invasive organisms pose a global threat and are exceptionally difficult to eradicate after they become abundant in their new habitats. We report a successful multitactic strategy for combating the pink bollworm (Pectinophora gossypiella), one of the world's most invasive pests. A coordinated program in the southwester...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tabashnik BE,Liesner LR,Ellsworth PC,Unnithan GC,Fabrick JA,Naranjo SE,Li X,Dennehy TJ,Antilla L,Staten RT,Carrière Y

更新日期：2021-01-05 00:00:00

abstract：:The P23T mutant of human gammaD-crystallin (HGD) is associated with cataract. We have previously investigated the solution properties of this mutant, as well as those of the closely related P23V and P23S mutants, and shown that although mutations at site 23 of HGD do not produce a significant structural change in the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McManus JJ,Lomakin A,Ogun O,Pande A,Basan M,Pande J,Benedek GB

更新日期：2007-10-23 00:00:00