Abstract:
:Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the C = N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml or urine.
journal_name
Proc Natl Acad Sci U S Aauthors
Aigner-Held R,Campbell RA,Daves GD Jrdoi
10.1073/pnas.76.12.6652subject
Has Abstractpub_date
1979-12-01 00:00:00pages
6652-5issue
12eissn
0027-8424issn
1091-6490journal_volume
76pub_type
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