Interprotofilament interactions between Alzheimer's Abeta1-42 peptides in amyloid fibrils revealed by cryoEM.

abstract：:In most genome-wide linkage studies, implication of a causative disease gene often requires years of expanding the study to more families and finer mapping of the initially described region. Even after such efforts, unobtainable sample sizes can be required to make statistically meaningful conclusions about a single g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Antinozzi PA,Garcia-Diaz A,Hu C,Rothman JE

更新日期：2006-03-07 00:00:00

abstract：:Interleukin-12 (IL-12) is critical for induction of protective immunity against intracellular bacterial infection. However, the mechanisms for efficient induction of IL-12 in innate response remain poorly understood. Here we report that the B type of carbonic anhydrase 6 (Car6-b, which encoded CA-VI B) is essential fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Xu X,Wang B,Ma Y,Zhang L,Xu H,Hu Y,Wu J,Cao X

更新日期：2017-08-08 00:00:00

abstract：:Type I (IFN-alpha/beta) and type III (IFN-lambdas) IFNs are important components of the host antiviral response. Although type III IFNs possess intrinsic antiviral activity similar to that of type I IFNs, they signal through a specific unique receptor complex, and their functional importance for antiviral resistance i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang J,Smirnov SV,Lewis-Antes A,Balan M,Li W,Tang S,Silke GV,Pütz MM,Smith GL,Kotenko SV

更新日期：2007-06-05 00:00:00

abstract：:AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein into proteoliposomes. In the presence of DeltapH (outside acid), the protein catalyzed the extrusion of fluorescent phosp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zgurskaya HI,Nikaido H

更新日期：1999-06-22 00:00:00

abstract：:The toxicological significance of exposures to synthetic chemicals is examined in the context of exposures to naturally occurring chemicals. We calculate that 99.99% (by weight) of the pesticides in the American diet are chemicals that plants produce to defend themselves. Only 52 natural pesticides have been tested in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ames BN,Profet M,Gold LS

更新日期：1990-10-01 00:00:00

abstract：:Microsomal NADPH-cytochrome P450 reductase (CPR) is one of only two mammalian enzymes known to contain both FAD and FMN, the other being nitric-oxide synthase. CPR is a membrane-bound protein and catalyzes electron transfer from NADPH to all known microsomal cytochromes P450. The structure of rat liver CPR, expressed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang M,Roberts DL,Paschke R,Shea TM,Masters BS,Kim JJ

更新日期：1997-08-05 00:00:00

abstract：:Aminoacyl-tRNA synthetases are multidomain proteins responsible for the attachment of specific amino acids to their tRNA substrates. Prolyl-tRNA synthetases (ProRSs) are notable due to their particularly diverse architectures through evolution. For example, Saccharomyces cerevisiae ProRS possesses an N-terminal extens...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： SternJohn J,Hati S,Siliciano PG,Musier-Forsyth K

更新日期：2007-02-13 00:00:00

abstract：:The molecular weight of the major glycoprotein from the human erythrocyte membrane is 29,000, of which 55% is carbohydrate and 45% is protein. The binding of sodium dodecyl sulfate to this glycoprotein is anomalous when compared to water soluble proteins and leads to migration rates in sodium dodecyl sulfate-polyacryl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grefrath SP,Reynolds JA

更新日期：1974-10-01 00:00:00

abstract：:Development of effective chemopreventive agents against prostate cancer (CaP) for humans requires conclusive evidence of their efficacy in animal models that closely emulates human disease. The autochthonous transgenic adenocarcinoma of the mouse prostate (TRAMP) model, which spontaneously develops metastatic CaP, is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gupta S,Hastak K,Ahmad N,Lewin JS,Mukhtar H

更新日期：2001-08-28 00:00:00

abstract：:Dopamine-beta-hydroxylase (EC 1.14.2.1), an enzyme localized in sympathetic synaptic vesicles, was released together with norepinephrine during in vitro stimulation of the hypogastric nerve that innervates the vas deferens of the guinea pig. Stimulation of the nerve in the presence of high concentrations of calcium (7...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson DG,Thoa NB,Weinshilboum R,Axelrod J,Kopin IJ

更新日期：1971-09-01 00:00:00

abstract：:Conventional wisdom regarding mechanisms of bacterial pathogenesis holds that pathogens arise by external acquisition of distinct virulence factors, whereas determinants shared by pathogens and commensals are considered to be functionally equivalent and have been ignored as genes that could become adapted specifically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sokurenko EV,Chesnokova V,Dykhuizen DE,Ofek I,Wu XR,Krogfelt KA,Struve C,Schembri MA,Hasty DL

更新日期：1998-07-21 00:00:00

abstract：:Phenotypic capacitance refers to the ability of a genome to accumulate mutations that are conditionally hidden and only reveal phenotype-altering effects after certain environmental or genetic changes. Capacitance has important implications for the evolution of novel forms and functions, but experimentally studied mec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hayden EJ,Bendixsen DP,Wagner A

更新日期：2015-10-06 00:00:00

abstract：:Defects in clearing apoptotic debris disrupt tissue and immunological homeostasis, leading to autoimmune and inflammatory diseases. Herein, we report that macrophages from lupus-prone MRL/lpr mice have impaired lysosomal maturation, resulting in heightened ROS production and attenuated lysosomal acidification. Impaire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Monteith AJ,Kang S,Scott E,Hillman K,Rajfur Z,Jacobson K,Costello MJ,Vilen BJ

更新日期：2016-04-12 00:00:00

abstract：:The synthetic polypeptide antigen poly(LTyr, LGlu)-poly(DLAl)- -poly(LLys)[T,G)-A- -L] was covalently linked to N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), which is the minimal adjuvant-active structure that can substitute for Mycobacteria in complete Freund's adjuvant. When injected in aqueous solution into mice, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mozes E,Sela M,Chedid L

更新日期：1980-08-01 00:00:00

abstract：:The mammalian retina contains an endogenous circadian pacemaker that broadly regulates retinal physiology and function, yet the cellular origin and organization of the mammalian retinal circadian clock remains unclear. Circadian clock neurons generate daily rhythms via cell-autonomous autoregulatory clock gene network...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan GX,Zhang DQ,Zhou T,Yamazaki S,McMahon DG

更新日期：2006-06-20 00:00:00

abstract：:Peroxisome proliferators cause rapid and coordinated transcriptional activation of genes encoding peroxisomal beta-oxidation system enzymes by activating peroxisome proliferator-activated receptor (PPAR) isoform(s). Since the thyroid hormone (T3; 3,3',5-triiodothyronine) receptor (TR), another member of the nuclear ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu R,Madison LD,Lin Y,Kopp P,Rao MS,Jameson JL,Reddy JK

更新日期：1995-12-05 00:00:00

abstract：:Ovine pulmonary surfactant is bactericidal for Pasteurella haemolytica when surfactant and bacteria mixtures are incubated with normal ovine serum. To isolate this component, surfactant (1 mg/ml) was centrifuged at 100,000 x gav, and the supernatant was fractionated by HPLC. Fractions were eluted with acetonitrile (10...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brogden KA,De Lucca AJ,Bland J,Elliott S

更新日期：1996-01-09 00:00:00

abstract：:The thymic microenvironment involves complex cell interactions among different types of epithelial cells, macrophages, tissue histiocytes, and immature and maturing T cells. We describe the isolation of a subset of thymic epithelial cells by selective primary culture followed by cotransfection with a simian virus 40 r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mizutani S,Watt SM,Robertson D,Hussein S,Healy LE,Furley AJ,Greaves MF

更新日期：1987-07-01 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a devastating human disease of upper and lower motoneurons of unknown etiology. In support of the potential role of autoimmunity in ALS, two immune-mediated animal models of motoneuron disease have been developed that resemble ALS with respect to the loss of motoneurons, the pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Appel SH,Engelhardt JI,García J,Stefani E

更新日期：1991-01-15 00:00:00

abstract：:In earlier studies it was shown that the mammalian translation system is highly organized in vivo and that the intermediates in the process, aminoacyl-tRNAs, are channeled--i.e., they are directly transferred from the aminoacyl-tRNA synthetases to the elongation factor to the ribosomes without dissociating into the ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stapulionis R,Deutscher MP

更新日期：1995-08-01 00:00:00

abstract：:Mast cells with the morphological and biochemical properties of mucosal mast cells (MMC) appear and proliferate to form the predominant cell type in rat bone marrow cultures stimulated with factors from antigen- or mitogen-activated lymphocytes. Conditioned media causing a selective proliferation of MMC were derived f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haig DM,McMenamin C,Gunneberg C,Woodbury R,Jarrett EE

更新日期：1983-07-01 00:00:00

abstract：:Targeting tyrosine kinase receptors (RTKs) with specific Abs is a promising therapeutic approach for cancer treatment, although the molecular mechanism(s) responsible for the Abs' biological activity are not completely known. We targeted the transmembrane RTK for hepatocyte growth factor (HGF) with a monoclonal Ab (DN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petrelli A,Circosta P,Granziero L,Mazzone M,Pisacane A,Fenoglio S,Comoglio PM,Giordano S

更新日期：2006-03-28 00:00:00

abstract：:The application of boron neutron capture therapy to rheumatoid arthritis requires the selective delivery of the boron-10 isotope to the synovitic tissue. The use of liposomes as a boron delivery method has been explored through the measurement of the time course biodistribution of boron in rats with collagen-induced a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watson-Clark RA,Banquerigo ML,Shelly K,Hawthorne MF,Brahn E

更新日期：1998-03-03 00:00:00

abstract：:A close correlation was observed between intracellular Ca2+ pool depletion and refilling and the onset of DNA synthesis and proliferation of DDT1MF-2 smooth muscle cells. The intracellular Ca2+ pump inhibitors 2,5-di-tert-butyl-hydroquinone (DBHQ) and thapsigargin (TG) specifically emptied identical inositol 1,4,5-tri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Short AD,Bian J,Ghosh TK,Waldron RT,Rybak SL,Gill DL

更新日期：1993-06-01 00:00:00

abstract：:This investigation was undertaken to determine the possible role of growth hormone (GH) in the hormonal regulation of hepatic low density lipoprotein (LDL) receptor expression. Treatment of normal rats with estrogen (ethynylestradiol, 5 mg/kg per day) increased the number of hepatic LDL receptors, and the LDL receptor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rudling M,Norstedt G,Olivecrona H,Reihnér E,Gustafsson JA,Angelin B

更新日期：1992-08-01 00:00:00

abstract：:Pokeweed antiviral protein (PAP), a 29-kDa protein isolated from Phytolacca americana, inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. In addition to its ribosome-inactivating ability, PAP has potent antiviral activity against ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tumer NE,Hwang DJ,Bonness M

更新日期：1997-04-15 00:00:00

abstract：:Most Escherichia coli promoters initiate transcription with a purine 7 or 8 nt downstream from the -10 hexamer, but some promoters, including the ribosomal RNA promoter rrnB P1, start 9 nt from the -10 element. We identified promoter and RNA polymerase determinants of this noncanonical rrnB P1 start site using biochem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winkelman JT,Chandrangsu P,Ross W,Gourse RL

更新日期：2016-03-29 00:00:00

abstract：:A series of hairpin pyrrole-imidazole polyamide-fluorescein conjugates were synthesized and assayed for cellular localization. Thirteen cell lines, representing 11 human cancers, one human transformed kidney cell line, and one murine leukemia cell line, were treated with 5 microM polyamide-fluorescein conjugates for 1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Best TP,Edelson BS,Nickols NG,Dervan PB

更新日期：2003-10-14 00:00:00

abstract：:Kinetic partitioning is predicted to be a general mechanism for proteins to fold into their well defined native three-dimensional structure from unfolded states following multiple folding pathways. However, experimental evidence supporting this mechanism is still limited. By using single-molecule atomic force microsco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peng Q,Li H

更新日期：2008-02-12 00:00:00

abstract：:Cells transformed by Friend virus in liquid suspension culture respond to low concentrations of dimethylsulfoxide by initiating hemoglobin synthesis. The kinetics of appearance of such differentiated erythroid cells is consistent with either the induction of differentiation in a uniformly susceptible population of tra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Singer D,Cooper M,Maniatis GM,Marks PA,Rifkind RA

更新日期：1974-07-01 00:00:00