Nonvesicular phospholipid transfer between peroxisomes and the endoplasmic reticulum.

abstract：:A fraction obtained by gel filtration at neutral pH of the extracellular Hb of Lumbricus terrestris dissociated either at pH 9.8 or at pH 4.0, consisting of the three subunits D1 (31 kDa), D2 (37 kDa), and T (50 kDa), was found to produce two peaks when subjected to gel filtration on Superose 6 at pH 7. The first peak...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kapp OH,Mainwaring MG,Vinogradov SN,Crewe AV

更新日期：1987-11-01 00:00:00

abstract：:The chromatin core particle DNA conformation deduced in broad outline by Finch et al. [Finch, J. T., Lutter, L. C., Rhodes, D., Brown, R. S., Rushton, B., Levitt, M. & Klug, A. (1977) Nature 269, 29-36] can be described in detail using other available experimental results. Histone binding sites compatible with the pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carter CW Jr

更新日期：1978-08-01 00:00:00

abstract：:Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved from beta-galactosida...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goeddel DV,Kleid DG,Bolivar F,Heyneker HL,Yansura DG,Crea R,Hirose T,Kraszewski A,Itakura K,Riggs AD

更新日期：1979-01-01 00:00:00

abstract：:There is evidence both for and against Na(+)- and Cl(-)-dependent neurotransmitter transporters forming oligomers. We found that cross-linking the human dopamine transporter (DAT), which is heterologously expressed in human embryonic kidney 293 cells, either with copper phenanthroline (CuP) or the bifunctional reagent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hastrup H,Karlin A,Javitch JA

更新日期：2001-08-28 00:00:00

abstract：:Phosphatase PP2A expression levels are positively correlated to the clinical severity of systemic lupus erythematosus (SLE) and IL17A cytokine overproduction, indicating a potential role of PP2A in controlling TH17 differentiation and inflammation. By generating a mouse strain with ablation of the catalytic subunit α ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu Q,Jin X,Zheng M,Rohila D,Fu G,Wen Z,Lou J,Wu S,Sloan R,Wang L,Hu H,Gao X,Lu L

更新日期：2019-01-15 00:00:00

abstract：:Benzophenones are among the most useful photocrosslinking agents in biology. We have evolved an orthogonal aminoacyl-tRNA synthetase/tRNA pair that makes possible the in vivo incorporation of p-benzoyl-l-phenylalanine into proteins in Escherichia coli in response to the amber codon, TAG. This unnatural amino acid was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chin JW,Martin AB,King DS,Wang L,Schultz PG

更新日期：2002-08-20 00:00:00

abstract：:A convergent, highly stereoselective total synthesis of (-)-spinosyn A (1) is described. Key features of the synthesis include the transannular Diels-Alder reaction of macrocyclic pentaene 11 and the transannular Morita-Baylis-Hillman cyclization of 12 that generates tetracycle 26. The total synthesis of (-)-spinosyn ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mergott DJ,Frank SA,Roush WR

更新日期：2004-08-17 00:00:00

abstract：:The genes encoding the histone acetyl-transferases (HATs) CREB binding protein (CREBBP) and EP300 are recurrently mutated in the activated B cell-like and germinal center (GC) B cell-like subtypes of diffuse large B cell lymphoma (DLBCL). Here, we introduced a patient mutation into a human DLBCL cell line using CRISPR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hashwah H,Schmid CA,Kasser S,Bertram K,Stelling A,Manz MG,Müller A

更新日期：2017-09-05 00:00:00

abstract：:Two-dimensional 1H NMR investigations were used to locate elements of regular secondary structure in the human complement protein C3a (the des-Arg77 derivative) in solution. The results were compared to a refined crystal structure based on the 3.2-A resolution structure of des-Arg77-C3a [Huber, R., Scholze, H., Paques...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nettesheim DG,Edalji RP,Mollison KW,Greer J,Zuiderweg ER

更新日期：1988-07-01 00:00:00

abstract：:Speech recognition involves three processes: extraction of acoustic indices from the speech signal, estimation of the probability that the observed index string was caused by a hypothesized utterance segment, and determination of the recognized utterance via a search among hypothesized alternatives. This paper is not ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jelinek F

更新日期：1995-10-24 00:00:00

abstract：:Previous work indicated that the light chains of a monotypic immunoglobulins G2-K and M-K from a single patient (Ti1) are identical. Our present data show that the monotypic immunoglobulins G and M share idiotypic determinants not present in their isolated light chains or in any of a large number of other immunoglobul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang AC,Wilson KS,Hopper JE,Fudenberg HH,Nisonoff A

更新日期：1970-06-01 00:00:00

abstract：:Taking advantage of a fluorescent Ca(2+) indicator selectively targeted to the trans-Golgi lumen, we here demonstrate that its Ca(2+) homeostatic mechanisms are distinct from those of the other Golgi subcompartments: (i) Ca(2+) uptake depends exclusively on the activity of the secretory pathway Ca(2+) ATPase1 (SPCA1),...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lissandron V,Podini P,Pizzo P,Pozzan T

更新日期：2010-05-18 00:00:00

abstract：:We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNF1) and 87,093 (pNF2) bp with average G+C ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishikawa J,Yamashita A,Mikami Y,Hoshino Y,Kurita H,Hotta K,Shiba T,Hattori M

更新日期：2004-10-12 00:00:00

abstract：:To address devastating environmental crises and to improve human well-being, China has been implementing a number of national policies on payments for ecosystem services. Two of them, the Natural Forest Conservation Program (NFCP) and the Grain to Green Program (GTGP), are among the biggest programs in the world becau...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu J,Li S,Ouyang Z,Tam C,Chen X

更新日期：2008-07-15 00:00:00

abstract：:Buffering variability in morphogen distribution is essential for reproducible patterning. A theoretically proposed class of mechanisms, termed "distal pinning," achieves robustness by combining local sensing of morphogen levels with global modulation of gradient spread. Here, we demonstrate a critical role for morphog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rahimi N,Carmon S,Averbukh I,Khajouei F,Sinha S,Schejter ED,Barkai N,Shilo BZ

更新日期：2020-01-21 00:00:00

abstract：:Nicotiana tabacum cv. Turkish Samsun NN plants were transformed with a modified and truncated replicase gene encoded by RNA-2 of cucumber mosaic virus strain Fny. The replicase gene had been modified by deleting a 94-base-pair region spanning nucleotides 1857-1950; the deletion also caused a shift in the open reading ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson JM,Palukaitis P,Zaitlin M

更新日期：1992-09-15 00:00:00

abstract：:Recently, hepatic peroxisome proliferator-activated receptor (PPAR)γ has been implicated in hepatic lipid accumulation. We found that the C3H mouse strain does not express PPARγ in the liver and, when subject to a high-fat diet, is resistant to hepatic steatosis, compared with C57BL/6 (B6) mice. Adenoviral PPARγ2 inje...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee YJ,Ko EH,Kim JE,Kim E,Lee H,Choi H,Yu JH,Kim HJ,Seong JK,Kim KS,Kim JW

更新日期：2012-08-21 00:00:00

abstract：:Disruption of neurotransmitter vesicle dynamics (transport, capacity, release) has been implicated in a variety of neurodegenerative and neuropsychiatric conditions. Here, we report a novel mouse model of enhanced vesicular function via bacterial artificial chromosome (BAC)-mediated overexpression of the vesicular mon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lohr KM,Bernstein AI,Stout KA,Dunn AR,Lazo CR,Alter SP,Wang M,Li Y,Fan X,Hess EJ,Yi H,Vecchio LM,Goldstein DS,Guillot TS,Salahpour A,Miller GW

更新日期：2014-07-08 00:00:00

abstract：:A disulfide bond that is evidently conserved in the guanine nucleotide-binding protein-coupled receptors is present in rhodopsin between Cys-110 and Cys-187. We have replaced these two cysteine residues by alanine residues and now report on the properties of the resulting rhodopsin mutants. The mutant protein C110A/C1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davidson FF,Loewen PC,Khorana HG

更新日期：1994-04-26 00:00:00

abstract：:Urinary acidification by the turtle bladder is due to a H+-ATPase that is located in the luminal membrane. The rate of H+ transport is stimulated by an increase in the ambient CO2. Using the fluorescent dye acridine orange, we showed that the mitochondria-rich cell of this equilibrium contains vesicles whose internal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gluck S,Cannon C,Al-Awqati Q

更新日期：1982-07-01 00:00:00

abstract：:We investigate the problem of learning to play the game of rock-paper-scissors. Each player attempts to improve her/his average score by adjusting the frequency of the three possible responses, using reinforcement learning. For the zero sum game the learning process displays Hamiltonian chaos. Thus, the learning traje...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sato Y,Akiyama E,Farmer JD

更新日期：2002-04-02 00:00:00

abstract：:Explanations for primate brain expansion and the evolution of human cognition and culture remain contentious despite extensive research. While multiple comparative analyses have investigated variation in brain size across primate species, very few have addressed why primates vary in how much they use social learning. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Street SE,Navarrete AF,Reader SM,Laland KN

更新日期：2017-07-25 00:00:00

abstract：:The water channel aquaporin 1 (AQP1) and certain Rh-family members are permeable to CO(2) and NH(3). Here, we use changes in surface pH (pH(S)) to assess relative CO(2) vs. NH(3) permeability of Xenopus oocytes expressing members of the AQP or Rh family. Exposed to CO(2) or NH(3), AQP1 oocytes exhibit a greater maxima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musa-Aziz R,Chen LM,Pelletier MF,Boron WF

更新日期：2009-03-31 00:00:00

abstract：:Mucopolysaccharidosis type VII is a lysosomal storage disorder resulting from inherited deficiency of beta-glucuronidase (GUS). Mucopolysaccharidosis type VII is characterized by glycosaminoglycan storage in most tissues, including brain. In these disorders, enzyme delivery across the blood-brain barrier (BBB) is the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Urayama A,Grubb JH,Sly WS,Banks WA

更新日期：2004-08-24 00:00:00

abstract：:An essential element in the web-trap architecture, the capture silk spun by ecribellate orb spiders consists of glue droplets sitting astride a silk filament. Mechanically this thread presents a mixed solid-liquid behavior unknown to date. Under extension, capture silk behaves as a particularly stretchy solid, owing t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elettro H,Neukirch S,Vollrath F,Antkowiak A

更新日期：2016-05-31 00:00:00

abstract：:cDNAs representing the alpha subunit of polyomavirus enhancer binding protein 2 (PEBP2; also called PEA2) were isolated. The products of the cDNAs are highly homologous to that of Drosophila segmentation gene runt (run) for an N-proximal 128-amino acid region showing 66% identity. The run homology region encompasses t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogawa E,Maruyama M,Kagoshima H,Inuzuka M,Lu J,Satake M,Shigesada K,Ito Y

更新日期：1993-07-15 00:00:00

abstract：:The peroxidation of polyunsaturated fatty acids leads to numerous products, including 4-hydroxynonenal (HNE). That 4-hydroxy-2-alkenal compounds react with sulfhydryl groups of proteins to form thioether adducts possessing a carbonyl function has been established [Schauenstein, E. & Esterbauer, H. (1979) Ciba Found. S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Uchida K,Stadtman ER

更新日期：1992-06-15 00:00:00

abstract：:Understanding the relationship between genetic variation and phenotypic variation for quantitative traits is necessary for predicting responses to natural and artificial selection and disease risk in human populations, but is challenging because of large sample sizes required to detect and validate loci with small eff...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swarup S,Huang W,Mackay TF,Anholt RR

更新日期：2013-01-15 00:00:00

abstract：:In epidemiological studies, both positive and negative correlations have been found between cardiovascular disease and mortality and the presence of several inorganic ions in the drinking water. In an attempt to resolve this apparent disagreement, we exposed White Carneau pigeons to drinking water containing calcium (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Revis NW,Zinsmeister AR,Bull R

更新日期：1981-10-01 00:00:00

abstract：:The Tsc2 gene, which is mutationally inactivated in the germ line of some families with tuberous sclerosis, encodes a large, membrane-associated GTPase activating protein (GAP) designated tuberin. Studies of the Eker rat model of hereditary cancer strongly support the role of Tsc2 as a tumor suppressor gene. In this s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin F,Wienecke R,Xiao GH,Maize JC Jr,DeClue JE,Yeung RS

更新日期：1996-08-20 00:00:00