Abstract:
:Benzophenones are among the most useful photocrosslinking agents in biology. We have evolved an orthogonal aminoacyl-tRNA synthetase/tRNA pair that makes possible the in vivo incorporation of p-benzoyl-l-phenylalanine into proteins in Escherichia coli in response to the amber codon, TAG. This unnatural amino acid was incorporated with high translational efficiency and fidelity into the dimeric protein glutathione S-transferase. Irradiation resulted in efficient crosslinking (>50%) of the protein subunits. This methodology may prove useful for discovering and defining protein interactions in vitro and in vivo.
journal_name
Proc Natl Acad Sci U S Aauthors
Chin JW,Martin AB,King DS,Wang L,Schultz PGdoi
10.1073/pnas.172226299keywords:
subject
Has Abstractpub_date
2002-08-20 00:00:00pages
11020-4issue
17eissn
0027-8424issn
1091-6490pii
172226299journal_volume
99pub_type
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