当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Intracellular distributions of mechanochemical proteins in cultured fibroblasts.

Intracellular distributions of mechanochemical proteins in cultured fibroblasts.

Abstract：

:We have used methods that have allowed simultaneous fluorescent staining of intracellular actin together with either myosin, filamin, or tubulin in normal rat kidney fibroblasts in monolayer culture. In the main portions of the cell body, the actin, myosin, and filamin are all present in two structures: in one, the three proteins are present in the same fiber bundles (stress fibers); in the other, there is a diffuse distribution of the three proteins. On portions of the cell periphery however-in the basal regions of microspikes, in ruffles, and in regions of cell-cell contact-actin and filamin are present, but myosin is severely depleted or absent. Microtubules are present in the cell body in a distribution independent of the stress fibers and are mostly absent from the cell periphery. Microspikes and ruffles are highly dynamic structures on the cell surface, and regions of cell-cell contact generally result from the association of ruffles on the two contacting cells. Therefore, the presence of filamin and actin but not myosin in these specialized regions on the cell surface, together with the recent demonstration [Wang, K. & Singer, S. J. (1977) Proc. Natl. Acad. Sci. USA 74, 2021-2025)] that pure filamin interacts with individual F-actin filaments in solution to form fiber bundles and sheet-like structures, suggest that in vivo filamin-actin interactions play an important role in the control of actin filament structure, in cell motility, and in the stabilization of cell-cell contacts.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Heggeness MH,Wang K,Singer SJ

doi

10.1073/pnas.74.9.3883

subject

Has Abstract

pub_date

1977-09-01 00:00:00

pages

3883-7

issue

9

eissn

0027-8424

issn

1091-6490

journal_volume

74

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Regulation of molecular components of the synapse in the developing and adult rat superior cervical ganglion.

    abstract：:Rat superior cervical sympathetic ganglion was used to begin studying the regulation of molecular components of the synapse. Ganglionic postsynaptic densities (PSDs)exhibited a thin, disc-shaped profile electron microscopically, comparable to that described for brain. Moreover, the presumptive ganglionic PSD protein (...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.23.8687

    authors： Wu K,Black IB

    更新日期：1987-12-01 00:00:00

  • ATM deficiency impairs thymocyte maturation because of defective resolution of T cell receptor alpha locus coding end breaks.

    abstract：:The ATM (ataxia telangiectasia mutated) protein plays a central role in sensing and responding to DNA double-strand breaks. Lymphoid cells are unique in undergoing physiologic double-strand breaks in the processes of Ig class switch recombination and T or B cell receptor V(D)J recombination, and a role for ATM in thes...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0611222104

    authors： Vacchio MS,Olaru A,Livak F,Hodes RJ

    更新日期：2007-04-10 00:00:00

  • High incidence of epithelial cancers in mice deficient for DNA polymerase delta proofreading.

    abstract：:Mutations are a hallmark of cancer. Normal cells minimize spontaneous mutations through the combined actions of polymerase base selectivity, 3' --> 5' exonucleolytic proofreading, mismatch correction, and DNA damage repair. To determine the consequences of defective proofreading in mammals, we created mice with a poin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.232340999

    authors： Goldsby RE,Hays LE,Chen X,Olmsted EA,Slayton WB,Spangrude GJ,Preston BD

    更新日期：2002-11-26 00:00:00

  • Moral consequences of becoming unemployed.

    abstract：:We test the conjecture that becoming unemployed erodes the extent to which a person acknowledges earned entitlement. We use behavioral experiments to generate incentive-compatible measures of individuals' tendencies to acknowledge earned entitlement and incorporate these experiments in a two-stage study. In the first ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1521250113

    authors： Barr A,Miller L,Ubeda P

    更新日期：2016-04-26 00:00:00

  • Hydrogen peroxide production, chemiluminescence, and the respiratory burst of fertilization: interrelated events in early sea urchin development.

    abstract：:After fertilization of the sea urchin, Strongyl-ocentrotus purpuratus, a crosslinked fertilization membrane is formed; the crosslinks (dityrosine residues) are synthesized in a reaction catalyzed by an ovoperoxidase that is released from the cortical granules during fertilization. The substrate for ovoperoxidase activ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.7.3183

    authors： Foerder CA,Klebanoff SJ,Shapiro BM

    更新日期：1978-07-01 00:00:00

  • Glutamate oxidation of 6C3HED lymphoma: effects of L-asparaginase on sensitive and resistant lines.

    abstract：:L-Asparaginase (EC 3.5.1.1) inhibited respiration in sensitive, but not resistant, lines of murine lymphoma 6C3HED. Glucose, in these tumor lines, was principally converted to lactate, and very little was oxidized in the citric acid cycle or hexose monophosphate shunt. The cells derived 70-80% of their respiratory CO(...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.10.3993

    authors： Lavietes BB,Regan DH,Demopoulos HB

    更新日期：1974-10-01 00:00:00

  • Monoclonal antibodies to the human insulin receptor block insulin binding and inhibit insulin action.

    abstract：:Antibodies to the insulin receptor were prepared in BALB/c mice by immunization with IM-9 human lymphocytes, a cell type that has a large number of plasma membrane insulin receptors. The spleens of these mice were then removed, and their lymphocytes were fused to a mouse myeloma cell line, FO cells. After screening ov...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.23.7312

    authors： Roth RA,Cassell DJ,Wong KY,Maddux BA,Goldfine ID

    更新日期：1982-12-01 00:00:00

  • Dysregulation of signaling pathways in CD45-deficient NK cells leads to differentially regulated cytotoxicity and cytokine production.

    abstract：:CD45, a protein tyrosine phosphatase that regulates Src family kinases, is important for regulating T cell and B cell receptor signaling; however, little is known about how CD45 regulates immunoreceptor tyrosine-based activation motif (ITAM)-dependent natural killer (NK) cell receptor signaling and the resulting effec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601851103

    authors： Hesslein DG,Takaki R,Hermiston ML,Weiss A,Lanier LL

    更新日期：2006-05-02 00:00:00

  • Liquid behavior of cross-linked actin bundles.

    abstract：:The actin cytoskeleton is a critical regulator of cytoplasmic architecture and mechanics, essential in a myriad of physiological processes. Here we demonstrate a liquid phase of actin filaments in the presence of the physiological cross-linker, filamin. Filamin condenses short actin filaments into spindle-shaped dropl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1616133114

    authors： Weirich KL,Banerjee S,Dasbiswas K,Witten TA,Vaikuntanathan S,Gardel ML

    更新日期：2017-02-28 00:00:00

  • Multiple genetic loci within 11p15 defined by Beckwith-Wiedemann syndrome rearrangement breakpoints and subchromosomal transferable fragments.

    abstract：:Beckwith-Wiedemann syndrome (BWS) involves fetal overgrowth and predisposition to a wide variety of embryonal tumors of childhood. We have previously found that BWS is genetically linked to 11p15 and that this same band shows loss of heterozygosity in the types of tumors to which children with BWS are susceptible. How...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.26.12456

    authors： Hoovers JM,Kalikin LM,Johnson LA,Alders M,Redeker B,Law DJ,Bliek J,Steenman M,Benedict M,Wiegant J,Lengauer C,Taillon-Miller P,Schlessinger D,Edwards MC,Elledge SJ,Ivens A,Westerveld A,Little P,Mannens M,Feinberg AP

    更新日期：1995-12-19 00:00:00

  • Reelin and glutamic acid decarboxylase67 promoter remodeling in an epigenetic methionine-induced mouse model of schizophrenia.

    abstract：:Reduction of prefrontal cortex glutamic acid decarboxylase (GAD67) and reelin (mRNAs and proteins) expression is the most consistent finding reported by several studies of postmortem schizophrenia (SZ) brains. Converging evidence suggests that the reduced GAD67 and reelin expression in cortical GABAergic interneurons ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505394102

    authors： Dong E,Agis-Balboa RC,Simonini MV,Grayson DR,Costa E,Guidotti A

    更新日期：2005-08-30 00:00:00

  • Receptors for oxidized low-density lipoprotein on elicited mouse peritoneal macrophages can recognize both the modified lipid moieties and the modified protein moieties: implications with respect to macrophage recognition of apoptotic cells.

    abstract：:It has been shown previously that the binding of oxidized low-density lipoprotein (OxLDL) to resident mouse peritoneal macrophages can be inhibited (up to 70%) by the apoprotein B (apoB) isolated from OxLDL, suggesting that macrophage recognition of OxLDL is primarily dependent on its modified protein moiety. However,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.11.6347

    authors： Bird DA,Gillotte KL,Hörkkö S,Friedman P,Dennis EA,Witztum JL,Steinberg D

    更新日期：1999-05-25 00:00:00

  • Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation.

    abstract：:Pyrrolysine (Pyl), the 22nd natural amino acid and genetically encoded by UAG, becomes attached to its cognate tRNA by pyrrolysyl-tRNA synthetase (PylRS). We have determined three crystal structures of the Methanosarcina mazei PylRS complexed with either AMP-PNP, Pyl-AMP plus pyrophosphate, or the Pyl analogue N-epsil...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0704769104

    authors： Kavran JM,Gundllapalli S,O'Donoghue P,Englert M,Söll D,Steitz TA

    更新日期：2007-07-03 00:00:00

  • Uncovering the hidden ground state of green fluorescent protein.

    abstract：:The fluorescence properties of GFP are strongly influenced by the protonation states of its chromophore and nearby amino acid side chains. In the ground state, the GFP chromophore is neutral and absorbs in the near UV. Upon excitation, the chromophore is deprotonated, and the resulting anionic chromophore emits its gr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0404262102

    authors： Kennis JT,Larsen DS,van Stokkum IH,Vengris M,van Thor JJ,van Grondelle R

    更新日期：2004-12-28 00:00:00

  • Mechanism of insertion of diphtheria toxin: peptide entry and pore size determinations.

    abstract：:Diphtheria toxin ( DTx ) is an extremely potent inhibitor of protein synthesis. It is secreted as a linear polypeptide, which is cleaved to produce disulfide-linked A and B fragments. Fragment A, the inhibitor of protein synthesis, requires fragment B, the recognition subunit, for entry into intact cells. Fragment B h...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.11.3341

    authors： Zalman LS,Wisnieski BJ

    更新日期：1984-06-01 00:00:00

  • Chromosomal drive and the evolution of meiotic nondisjunction and trisomy in humans.

    abstract：:Trisomy is a genetic abnormality of considerable medical importance. The most familiar example is trisomy 21, which causes Down Syndrome [Cummings, M. R. (1988) Human Heredity: Principles and Issues (West Publishing Company, New York)]. In a classic paper, Axelrod and Hamilton [Axelrod, R. & Hamilton, W. D. (1981) Sci...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.5.2361

    authors： Day T,Taylor PD

    更新日期：1998-03-03 00:00:00

  • Replicator degrees of freedom allow publication of misleading failures to replicate.

    abstract：:In recent years, the field of psychology has begun to conduct replication tests on a large scale. Here, we show that "replicator degrees of freedom" make it far too easy to obtain and publish false-negative replication results, even while appearing to adhere to strict methodological standards. Specifically, using data...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1910951116

    authors： Bryan CJ,Yeager DS,O'Brien JM

    更新日期：2019-12-17 00:00:00

  • In vitro transcription of eukaryotic genes is affected differently by the degree of DNA supercoiling.

    abstract：:In a posterior silk gland extract, covalently closed circular (ccc) DNA is in a superhelical state that supports more transcription of fibroin gene than does linear DNA. A HeLa cell extract showed neither the supercoiling activity nor the preference for the transcription of ccc DNA over linear DNA. These activities co...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.3.718

    authors： Hirose S,Suzuki Y

    更新日期：1988-02-01 00:00:00

  • A semiquinone intermediate generated at the Qo site of the cytochrome bc1 complex: importance for the Q-cycle and superoxide production.

    abstract：:The cytochrome bc1 and related complexes are essential energy-conserving components of mitochondrial and bacterial electron transport chains. They orchestrate a complex sequence of electron and proton transfer reactions resulting in the oxidation of quinol, the reduction of a mobile electron carrier, and the transloca...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702621104

    authors： Cape JL,Bowman MK,Kramer DM

    更新日期：2007-05-08 00:00:00

  • Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.

    abstract：:The ribosomal RNA genes (rDNA) of Tetrahymena thermophila macronucleus exist as free linear 21-kilobase molecules that contain replication origins and telomeres. A mutation in this gene confers resistance to the antibiotic paromomycin. We have isolated rDNA from such a mutant (strain p2f), microinjected it into the ma...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.12.4369

    authors： Tondravi MM,Yao MC

    更新日期：1986-06-01 00:00:00

  • Blimp1-mediated repression of negative regulators is required for osteoclast differentiation.

    abstract：:Regulation of irreversible cell lineage commitment depends on a delicate balance between positive and negative regulators, which comprise a sophisticated network of transcription factors. Receptor activator of NF-kappaB ligand (RANKL) stimulates the differentiation of bone-resorbing osteoclasts through the induction o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912779107

    authors： Nishikawa K,Nakashima T,Hayashi M,Fukunaga T,Kato S,Kodama T,Takahashi S,Calame K,Takayanagi H

    更新日期：2010-02-16 00:00:00

  • Autophosphorylation reversibly regulates the Ca2+/calmodulin-dependence of Ca2+/calmodulin-dependent protein kinase II.

    abstract：:Ca2+/calmodulin-dependent protein kinase II contains two subunits, alpha (Mr 50,000) and beta (Mr 60,000/58,000), both of which undergo Ca2+/calmodulin-dependent autophosphorylation. In the present study, we have studied the mechanism of this autophosphorylation reaction and its effect on the activity of the enzyme. B...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.12.4253

    authors： Lai Y,Nairn AC,Greengard P

    更新日期：1986-06-01 00:00:00

  • Pituitary growth hormone network responses are sexually dimorphic and regulated by gonadal steroids in adulthood.

    abstract：:There are well-recognized sex differences in many pituitary endocrine axes, usually thought to be generated by gonadal steroid imprinting of the neuroendocrine hypothalamus. However, the recognition that growth hormone (GH) cells are arranged in functionally organized networks raises the possibility that the responses...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1010849107

    authors： Sanchez-Cardenas C,Fontanaud P,He Z,Lafont C,Meunier AC,Schaeffer M,Carmignac D,Molino F,Coutry N,Bonnefont X,Gouty-Colomer LA,Gavois E,Hodson DJ,Le Tissier P,Robinson IC,Mollard P

    更新日期：2010-12-14 00:00:00

  • Generic theory for channel sinuosity.

    abstract：:Sinuous patterns traced by fluid flows are a ubiquitous feature of physical landscapes on Earth, Mars, the volcanic floodplains of the Moon and Venus, and other planetary bodies. Typically discussed as a consequence of migration processes in meandering rivers, sinuosity is also expressed in channel types that show lit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1214074110

    authors： Lazarus ED,Constantine JA

    更新日期：2013-05-21 00:00:00

  • Lessons learned and concepts formed from study of the pathogenesis of the two negative-strand viruses lymphocytic choriomeningitis and influenza.

    abstract：:Viruses have unique lifestyles. To describe the pathogenesis and significance of viral infection in terms of host responses, resultant injury, and therapy, we focused on two RNA viruses: lymphocytic choriomeningitis (LCMV) and influenza (Flu). Many of the currently established concepts and consequences about viruses a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.1222025110

    authors： Oldstone MB

    更新日期：2013-03-12 00:00:00

  • The tRNA processing enzyme RNase T is essential for maturation of 5S RNA.

    abstract：:The maturation of 5S RNA in Escherichia coli is poorly understood. Although it is known that large precursors of 5S RNA accumulate in mutant cells lacking the endoribonuclease-RNase E, almost nothing is known about how the mature 5' and 3' termini of these molecules are generated. We have examined 5S RNA maturation in...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.15.6883

    authors： Li Z,Deutscher MP

    更新日期：1995-07-18 00:00:00

  • RNase T is responsible for the end-turnover of tRNA in Escherichia coli.

    abstract：:A mutant strain deficient in RNase T was isolated and used to study the role of this enzyme in Escherichia coli. Strains lacking as much as 70% of RNase T activity, alone or in combination with the absence of other RNases, display normal growth properties. However, in cca strains, which lack tRNA nucleotidyltransferas...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.19.6427

    authors： Deutscher MP,Marlor CW,Zaniewski R

    更新日期：1985-10-01 00:00:00

  • Cytotoxic T cells: Lyt phenotype and blocking of killing activity by Lyt antisera.

    abstract：:WE REEXAMINED TWO QUESTIONS CONCERNING LYT ANTIGENS OF CYTOTOXIC T CELLS OF THE MOUSE: is Lyt-1 antigen expressed on cytotoxic effector cells and can cytotoxicity be blocked by antibody to Lyt antigens in the absence of added complement? A 3-hr (51)Cr-release assay with splenic effector cells and leukemia or myeloma t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.4.1977

    authors： Nakayama E,Shiku H,Stockert E,Oettgen HF,Old LJ

    更新日期：1979-04-01 00:00:00

  • Global variability in seawater Mg:Ca and Sr:Ca ratios in the modern ocean.

    abstract：:Seawater Mg:Ca and Sr:Ca ratios are biogeochemical parameters reflecting the Earth-ocean-atmosphere dynamic exchange of elements. The ratios' dependence on the environment and organisms' biology facilitates their application in marine sciences. Here, we present a measured single-laboratory dataset, combined with previ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1918943117

    authors： Lebrato M,Garbe-Schönberg D,Müller MN,Blanco-Ameijeiras S,Feely RA,Lorenzoni L,Molinero JC,Bremer K,Jones DOB,Iglesias-Rodriguez D,Greeley D,Lamare MD,Paulmier A,Graco M,Cartes J,Barcelos E Ramos J,de Lara A,Sanchez-Lea

    更新日期：2020-09-08 00:00:00

  • In vivo imaging reveals that pregabalin inhibits cortical spreading depression and propagation to subcortical brain structures.

    abstract：:Migraine is characterized by severe headaches that can be preceded by an aura likely caused by cortical spreading depression (SD). The antiepileptic pregabalin (Lyrica) shows clinical promise for migraine therapy, although its efficacy and mechanism of action are unclear. As detected by diffusion-weighted MRI (DW-MRI)...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1614447114

    authors： Cain SM,Bohnet B,LeDue J,Yung AC,Garcia E,Tyson JR,Alles SR,Han H,van den Maagdenberg AM,Kozlowski P,MacVicar BA,Snutch TP

    更新日期：2017-02-28 00:00:00