Spontaneous driving forces give rise to protein-RNA condensates with coexisting phases and complex material properties.

abstract：:Solid-state cross-polarization/magic-angle sample-spinning (13)C NMR spectra have been recorded on chlorophyll a-water aggregates, methyl pyrochlorophyllide a, and methyl pyropheophorbide a (derivatives that lack a phytyl chain). Spectra have also been collected under a decoupling regime in which resonances of certain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown CE,Spencer RB,Burger VT,Katz JJ

更新日期：1984-01-01 00:00:00

abstract：:Although Hsp90 displays general chaperone activity in vitro, few substrates of the chaperone have been identified in vivo, and the characteristics that render these substrates dependent on Hsp90 remain elusive. To investigate this issue, we exploited a paradoxical observation: several unrelated oncogenic viral tyrosin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu Y,Singer MA,Lindquist S

更新日期：1999-01-05 00:00:00

abstract：:Novel cellular behaviors and characteristics can be obtained by coupling engineered gene networks to the cell's natural regulatory circuitry through appropriately designed input and output interfaces. Here, we demonstrate how an engineered genetic circuit can be used to construct cells that respond to biological signa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobayashi H,Kaern M,Araki M,Chung K,Gardner TS,Cantor CR,Collins JJ

更新日期：2004-06-01 00:00:00

abstract：:After exposure to low density lipoprotein (LDL) that had been minimally modified by oxidation (MM-LDL), human endothelial cells (EC) and smooth muscle cells (SMC) cultured separately or together produced 2- to 3-fold more monocyte chemotactic activity than did control cells or cells exposed to freshly isolated LDL. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cushing SD,Berliner JA,Valente AJ,Territo MC,Navab M,Parhami F,Gerrity R,Schwartz CJ,Fogelman AM

更新日期：1990-07-01 00:00:00

abstract：:We have investigated the folding dynamics of Thermus thermophilus cytochrome c(552) by time-resolved fluorescence energy transfer between the heme and each of seven site-specific fluorescent probes. We have found both an equilibrium unfolding intermediate and a distinct refolding intermediate from kinetics studies. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada S,Bouley Ford ND,Keller GE,Ford WC,Gray HB,Winkler JR

更新日期：2013-01-29 00:00:00

abstract：:Serial passage of simian virus 40 (SV40) at high multiplicity of infection leads to the emergence of variants with deleted, substituted, and/or duplicated DNA. Individual variants have been cloned by selective complementation with temperature sensitive SV40 mutants, or nonselectively by coinfection of cells with wild-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brockman WW,Nathans D

更新日期：1974-03-01 00:00:00

abstract：:Actin purified from mouse fibroblasts by sodium dodecyl sulfate gel electrophoresis was used as antigen to obtain an antibody in rabbits. The elicited antibody was shown to be specific for actin as judged by immunodiffusion and complement fixation against partially purified mouse fibroblast actin and highly purified c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lazarides E,Weber K

更新日期：1974-06-01 00:00:00

abstract：:How cells achieve their final sizes is a pervasive biological question. One strategy to increase cell size is for the cell to amplify its chromosomal DNA content through endoreduplication cycles. Although endoreduplication is widespread in eukaryotes, we know very little about its molecular mechanisms. Successful prog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugimoto-Shirasu K,Roberts GR,Stacey NJ,McCann MC,Maxwell A,Roberts K

更新日期：2005-12-20 00:00:00

abstract：:Homozygous uniparental mouse embryos, produced by microsurgical removal of the male pronucleus from fertilized eggs and diploidization of the female pronucleus with cytochalasin, were surrounded with blastomeres from normal embryos to produce chimeric embryos. A few of these chimeras developed into viable adults, and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderegg C,Markert CL

更新日期：1986-09-01 00:00:00

abstract：:A series of monoclonal antibodies was used to define three discrete stages of human intrathymic T-cell differentiation. The earliest stage was confined to <10% of thymocytes, which were.reactive with both OKT9 and OKT10. Subsequently, approximately 70% of human thymocytes acquired a thymocyte-restricted antigen, OKT6,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinherz EL,Kung PC,Goldstein G,Levey RH,Schlossman SF

更新日期：1980-03-01 00:00:00

abstract：:EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Timmis K,Cabello F,Cohen SN

更新日期：1975-06-01 00:00:00

abstract：:p19ARF has been implicated as a key regulator of p53 stability and activation. While numerous stresses activate the p53 growth arrest pathway, those requiring p19ARF remain to be elucidated. We used p19ARF knockout mouse embryo fibroblasts to show that DNA damage and microtubule disruption require p19ARF to induce p53...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khan SH,Moritsugu J,Wahl GM

更新日期：2000-03-28 00:00:00

abstract：:The class of neurons within the visual cortex of normal adult cats that has the smallest receptive fields (less than or equal to 2.25 degrees2) and that responds only to low rates of stimulus motion (less than or equal to 50 degrees / sec) responds preferentially to lines oriented about either the horizontal axis (+/-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leventhal AG,Hirsch HV

更新日期：1977-03-01 00:00:00

abstract：:The first step in poliovirus replication is binding of virus to a cellular receptor. Mouse L cells, which are resistant to poliovirus infection because they do not bear a poliovirus receptor, were transformed with HeLa cell (human) DNA to poliovirus sensitivity at a frequency of approximately 1 in 50,000 transformants...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mendelsohn C,Johnson B,Lionetti KA,Nobis P,Wimmer E,Racaniello VR

更新日期：1986-10-01 00:00:00

abstract：:The S region of the murine major histocompatibility complex contains two structurally related genes (21-OHase A and 21-OHase B) that encode 21-hydroxylase (21-OHase), an enzyme essential for the synthesis of adrenal steroids. Expression of these two genes has been analyzed by using oligonucleotide probes specific for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker KL,Chaplin DD,Wong M,Seidman JG,Smith JA,Schimmer BP

更新日期：1985-12-01 00:00:00

abstract：:Genetic studies of the white locus have shown that it has a distal region where structural mutations occur and a proximal region where regulatory mutations occur. To better understand the molecular basis of this genetic organization we have analyzed white locus transcription. A 2.7-kilobase transcript comprising 0.000...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'hare K,Levis R,Rubin GM

更新日期：1983-11-01 00:00:00

abstract：:Human appropriation of net primary production (HANPP), the aggregate impact of land use on biomass available each year in ecosystems, is a prominent measure of the human domination of the biosphere. We present a comprehensive assessment of global HANPP based on vegetation modeling, agricultural and forestry statistics...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haberl H,Erb KH,Krausmann F,Gaube V,Bondeau A,Plutzar C,Gingrich S,Lucht W,Fischer-Kowalski M

更新日期：2007-07-31 00:00:00

abstract：:A vaccinia virus host range mutant (hr mutant) deleted of 18 kilobase pairs at the left end of the genome has been employed to precisely map a viral gene required for multiplication in human cells. DNA fragments from the wild-type virus were inserted into the thymidine kinase gene of mutant virus by means of in vivo h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gillard S,Spehner D,Drillien R,Kirn A

更新日期：1986-08-01 00:00:00

abstract：:Estrogen signaling occurs through at least two distinct molecular pathways: (i) direct binding of liganded estrogen receptors (ERs) to estrogen-responsive DNA elements (EREs) (the "ER/ERE pathway") and (ii) indirect recruitment of liganded ERs to activating protein-1 (AP-1)-responsive DNA elements via heterodimers of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung E,Acevedo ML,Cole PA,Kraus WL

更新日期：2005-01-18 00:00:00

abstract：:8-Azido[2-3H]adenine was used as a photoaffinity label for the purine-cytosine transport system. After irradiation in the presence of the photoaffinity label, the cells were converted into protoplasts, their plasma membranes were purified, and the membrane proteins were extracted and separated by NaDodSO4/PAGE. The ra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt R,Manolson MF,Chevallier MR

更新日期：1984-10-01 00:00:00

abstract：:Plasmons, the collective oscillations of interacting electrons, possess emergent properties that dramatically alter the optical response of metals. We predict the existence of a new class of plasmons-chiral Berry plasmons (CBPs)-for a wide range of 2D metallic systems including gapped Dirac materials. As we show, in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song JC,Rudner MS

更新日期：2016-04-26 00:00:00

abstract：:The kainate receptor (KAR), a subtype of glutamate receptor, mediates excitatory synaptic responses at a subset of glutamatergic synapses. However, the molecular mechanisms underlying the trafficking of its different subunits are poorly understood. Here we use the CA1 hippocampal pyramidal cell, which lacks KAR-mediat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheng N,Shi YS,Nicoll RA

更新日期：2017-01-31 00:00:00

abstract：:The monoclonal antibodies (mAb) DA6.147, DA6.164, and HIG.48 against human Ia antigens, but not the W6/32 mAb against human class I major histocompatibility complex antigens or the anti-monocyte OKM1 and 63D3 mAb, stimulated monocytes to secrete interleukin 1 (IL-1). IL-1 was measured by its property of promoting the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palacios R

更新日期：1985-10-01 00:00:00

abstract：:P450c17 is the single enzyme mediating both 17 alpha-hydroxylase (steroid 17 alpha-monooxygenase, EC 1.14.99.9) and 17,20 lyase activities in the synthesis of steroid hormones. It has been suggested that different P450c17 isozymes mediate these activities in the adrenal gland and testis. We sequenced 423 of the 509 am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung BC,Picado-Leonard J,Haniu M,Bienkowski M,Hall PF,Shively JE,Miller WL

更新日期：1987-01-01 00:00:00

abstract：:Strongylocentrotus intermedius (A. Agassiz, 1863) is an economically important sea urchin inhabiting the northwest Pacific region of Asia. The northern Primorye (Sea of Japan) populations of S. intermedius consist of two sympatric morphological forms, "usual" (U) and "gray" (G). The two forms are significantly differe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balakirev ES,Pavlyuchkov VA,Ayala FJ

更新日期：2008-10-21 00:00:00

abstract：:We examined the distributions of short tandemly repeated DNAs (microsatellites) in nine complete microbial genomes (Saccharomyces cerevisiae, Archaeoglobus fulgidus, Escherichia coli, Haemophilus influenzae, Helicobacter pylori, Methanococcus jannaschii, Mycoplasma pneumoniae, M. genitalium, and Synechocystis PCC6803....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Field D,Wills C

更新日期：1998-02-17 00:00:00

abstract：:Specific rearrangement of internal chromosomal regions occurs during development of the somatic macronucleus in Tetrahymena thermophila and results in elimination of germ-line (micronuclear) DNA sequences. The timing and mechanism of genome rearrangement within one particular 9.3-kilobase region, which contains three ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Austerberry CF,Allis CD,Yao MC

更新日期：1984-12-01 00:00:00

abstract：:A model for protein synthesis is proposed in which the donor for the peptide elongation reaction is peptidyl-5S RNA. Space-filling models show that peptide bond formation between peptidyl-5S RNA and aminoacyl-tRNA is eminently feasible from a stereochemical point of view. The peptide is transferred to 5S RNA, while at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raacke ID

更新日期：1971-10-01 00:00:00

abstract：:To use recombinant DNA technology to functionally analyze mutations introduced into cloned eukaryotic genes, a rapid procedure is necessary to assay the steps along the gene expression pathway. Since cloned rat insulin genes are not transcribed efficiently after transfection into various cell lines, I have asked wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lomedico PT

更新日期：1982-10-01 00:00:00

abstract：:Recent technical advances allow detection of several hundred volatile organic compounds (VOCs) in human exhaled air, many of which reflect unidentified endogenous pathways. Our group has previously estimated plasma glucose levels in healthy adults during a standard oral glucose tolerance test via exhaled VOC analysis....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novak BJ,Blake DR,Meinardi S,Rowland FS,Pontello A,Cooper DM,Galassetti PR

更新日期：2007-10-02 00:00:00