Quinacrine promotes replication and conformational mutation of chronic wasting disease prions.

abstract：:A Raleigh, NC, population of Drosophila melanogaster was sampled for the presence of enzyme null alleles at 25 loci. No nulls were found at any of five X-linked loci. Nulls were recovered at 13 of 20 autosomal loci; the weighted mean frequency for all 20 autosomal loci was estimated to be 0.0025. A consideration of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voelker RA,Langley CH,Brown AJ,Ohnishi S,Dickson B,Montgomery E,Smith SC

更新日期：1980-02-01 00:00:00

abstract：:Overproduction or deficiency of many chaperones and other cellular components cure the yeast prions [PSI+] (formed by Sup35p) or [URE3] (based on Ure2p). However, at normal expression levels, Btn2p and Cur1p eliminate most newly arising [URE3] variants but do not cure [PSI+], even after overexpression. Deficiency or o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorkovskiy A,Reidy M,Masison DC,Wickner RB

更新日期：2017-05-23 00:00:00

abstract：:Recent functional imaging studies have revealed coactivation in a distributed network of cortical regions that characterizes the resting state, or default mode, of the human brain. Among the brain regions implicated in this network, several, including the posterior cingulate cortex and inferior parietal lobes, have al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greicius MD,Srivastava G,Reiss AL,Menon V

更新日期：2004-03-30 00:00:00

abstract：:Sensitization of defensive gill and siphon withdrawal reflexes in Aplysia results, in part, from presynaptic facilitation of transmitter release from mechanoreceptor sensory neurons that innervate the siphon skin and synapse with interneurons and motor neurons. Presynaptic facilitation also can be elicited by applicat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hochner B,Klein M,Schacher S,Kandel ER

更新日期：1986-11-01 00:00:00

abstract：:Understanding the complexity of anisotropic turbulent processes over a wide range of spatiotemporal scales in engineering shear turbulence as well as climate atmosphere ocean science is a grand challenge of contemporary science with important societal impact. In such inhomogeneous turbulent dynamical systems there is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Majda AJ

更新日期：2015-07-21 00:00:00

abstract：:Effects of thyrotropin-releasing hormone (TRH; thyroliberin) on membrane electrical properties were studied in the clonal rat anterior pituitary cell (GH3) by continuous recording of the intracellular potential. Application of TRH, which stimulates the release of prolactin and growth hormone from the GH3 cell, elicite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozawa S,Kimura N

更新日期：1979-11-01 00:00:00

abstract：:A cDNA of the human terminal deoxynucleotidyltransferase (TdT; "terminal transferase," EC 2.7.7.31) was isolated from a human lymphoblastoid cell cDNA library in lambda gt 11 by using immunological procedures. Four inserts containing 723 to 939 base pairs were recloned in pBR322 for hybridization and preliminary seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peterson RC,Cheung LC,Mattaliano RJ,Chang LM,Bollum FJ

更新日期：1984-07-01 00:00:00

abstract：:By use of the indirect peroxidase-antiperoxidase complex immunocytochemical technique, antibody to purified human renal renin was applied to formalin-fixed paraffin sections of human cadaver brain. Immune reaction products were observed in most nerve cells in all areas of the brain examined; staining was limited to th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slater EE,Defendini R,Zimmerman EA

更新日期：1980-09-01 00:00:00

abstract：:We investigate the hypothesis that two classes of Ca2+ currents, one quickly inactivated by Ca2+ and one slowly inactivated by voltage, contribute to bursting electrical activity in pancreatic islets. A mathematical model of these currents is fit to the experimental whole-cell current-voltage and inactivation profiles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keizer J,Smolen P

更新日期：1991-05-01 00:00:00

abstract：:A three-step purification procedure for the isolation of heart muscle chalones is described. The method uses ultrafiltration followed by concanavalin A-Sepharose affinity chromatography and subsequent sucrose density gradient centrifugation. Analytical ultracentrifugation of the highly purified chalone fraction indica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kriek JA,van der Walt BJ,Bester AJ

更新日期：1981-07-01 00:00:00

abstract：:Neuroblastoma-glioma hybrid cells (NG108-15) in suspension accumulate the permeant lipophilic cation [(3)H]tetraphenylphosphonium (TPP(+)) against a concentration gradient. The steady-state level of TPP(+) accumulation is about twice as great in physiological media of low K(+) concentration (i.e., 5 mM K(+)/135 mM Na(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lichtshtein D,Kaback HR,Blume AJ

更新日期：1979-02-01 00:00:00

abstract：:A hierarchy of residue density assessments and packing properties in protein structures are contrasted, including a regular density, a variety of charge densities, a hydrophobic density, a polar density, and an aromatic density. These densities are investigated by alternative distance measures and also at the interfac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baud F,Karlin S

更新日期：1999-10-26 00:00:00

abstract：:Alzheimer's disease (AD) is characterized by a progressive dysfunction of central neurons. Recent experimental evidence indicates that in the cortex, in addition to the silencing of a fraction of neurons, other neurons are hyperactive in amyloid-β (Aβ) plaque-enriched regions. However, it has remained unknown what com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busche MA,Chen X,Henning HA,Reichwald J,Staufenbiel M,Sakmann B,Konnerth A

更新日期：2012-05-29 00:00:00

abstract：:In this era of unprecedented biodiversity loss and increased zoonotic disease emergence, it is imperative to understand the effects of biodiversity on zoonotic pathogen dynamics in wildlife. Whether increasing biodiversity should lead to a decrease or increase in infection prevalence, termed the dilution and amplifica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luis AD,Kuenzi AJ,Mills JN

更新日期：2018-07-31 00:00:00

abstract：:Inhibition of protein synthesis in rabbit reticulocyte lysates by double-stranded RNA is caused by the inactivation of IF-3, an initiation factor required for the recycling of ribosomes and for their binding to messenger RNA. The evidence for this is that (i) the inhibition can be overcome by addition of exogenous If-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaempfer R,Kaufman J

更新日期：1973-04-01 00:00:00

abstract：:Antagonists of growth hormone-releasing hormone (GHRH) inhibit the proliferation of various human cancers in vitro and in vivo by mechanisms that include apparent direct effects through specific binding sites expressed on tumors and that differ from pituitary human GHRH (hGHRH) receptors. In this study, GHRH antagonis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Halmos G,Schally AV,Varga JL,Plonowski A,Rekasi Z,Czompoly T

更新日期：2000-09-12 00:00:00

abstract：:Diabetic peripheral neuropathy (DPN) is the most common complication in both type 1 and type 2 diabetes. Here we studied some phenotypic features of a well-established animal model of type 2 diabetes, the leptin receptor-deficient db(-)/db(-) mouse, and also the effect of long-term (6 mo) treatment with coenzyme Q10 (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi TJ,Zhang MD,Zeberg H,Nilsson J,Grünler J,Liu SX,Xiang Q,Persson J,Fried KJ,Catrina SB,Watanabe M,Arhem P,Brismar K,Hökfelt TG

更新日期：2013-01-08 00:00:00

abstract：:In mantle cell lymphoma (MCL), the translocation t(11;14) is considered the cytogenetic hallmark of the disease. Recently, however, deletion of the chromosomal region 11q22-q23 has been identified as a frequent event in this type of cancer, indicating the existence of a pathogenically relevant tumor suppressor gene in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaffner C,Idler I,Stilgenbauer S,Döhner H,Lichter P

更新日期：2000-03-14 00:00:00

abstract：:Pancreatic beta cells respond to changes in blood glucose by secreting insulin and increasing insulin synthesis. To identify genes used in these responses, we have carried out expression profiling of beta cells exposed to high (25 mM) or low (5.5 mM) glucose by using oligonucleotide microarrays. Functional clustering ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webb GC,Akbar MS,Zhao C,Steiner DF

更新日期：2000-05-23 00:00:00

abstract：:Three proteins from cyanobacteria (KaiA, KaiB, and KaiC) can reconstitute circadian oscillations in vitro. At least three molecular properties oscillate during this reaction, namely rhythmic phosphorylation of KaiC, ATP hydrolytic activity of KaiC, and assembly/disassembly of intermolecular complexes among KaiA, KaiB,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin X,Byrne M,Mori T,Zou P,Williams DR,McHaourab H,Johnson CH

更新日期：2010-08-17 00:00:00

abstract：:Transabdominal (Tab) is a dominant gain-of-function mutation that results in islands of sexually dimorphic abdominal cuticle in the dorsal thorax of the adult fly. This phenotype has complete penetrance and constant expressivity, and we show that it results from ectopic expression of ABD-BII, one of two proteins deriv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Celniker SE,Lewis EB

更新日期：1993-02-15 00:00:00

abstract：:The switch from fetal to adult hemoglobin expression is regulated in many mammalian species by a developmental clock-like mechanism and determined by the gestational age of the fetus. Prolonging fetal globin gene expression is of considerable interest for therapeutic potential in diseases caused by abnormal beta-globi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perrine SP,Rudolph A,Faller DV,Roman C,Cohen RA,Chen SJ,Kan YW

更新日期：1988-11-01 00:00:00

abstract：:One of the factors required for the antiviral activity of the synthetic nucleoside, ribavirin (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), is the ability of the molecule to adopt the substrate conformation specified by the enzyme for which it is a competitive inhibitor, inosine 5'-phosphate dehydrogenase (IM...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DL,Miles DW,Redington P,Eyring H

更新日期：1976-12-01 00:00:00

abstract：:The influx of Ca ions into the presynaptic nerve terminal through ion channels is a key link between the action potential and the release of chemical transmitters. It is not clear, however, which types of Ca channel are involved in neurosecretion at vertebrate synapses. In particular, there is disagreement as to wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanley EF,Atrakchi AH

更新日期：1990-12-01 00:00:00

abstract：:A central tenet in understanding the biological effects of ionizing radiation has been that the initially affected cells were directly damaged by the radiation. By contrast, evidence has emerged concerning "bystander" responses involving damage to nearby cells that were not themselves directly traversed by the radiati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Belyakov OV,Mitchell SA,Parikh D,Randers-Pehrson G,Marino SA,Amundson SA,Geard CR,Brenner DJ

更新日期：2005-10-04 00:00:00

abstract：:DNA was prepared from 15 different mouse and rat cell lines transformed by chemical carcinogens in vitro and in vivo. These DNAs were applied to NIH3T3 mouse fibroblast cultures by using the calcium phosphate transfection technique. DNAs of five donor lines were able to induce foci on the recipient monolayers. Ten oth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shih C,Shilo BZ,Goldfarb MP,Dannenberg A,Weinberg RA

更新日期：1979-11-01 00:00:00

abstract：:The earliest measurable parameter that shows density-dependent inhibition is the uptake of [(3)H]deoxyglucose by Balb 3T3 cells. The rate decreases even during the exponential phase of growth and reaches a minimum, about 8- to 10-times lower than maximum, as the culture approaches the saturation density. Cells transfo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bose SK,Zlotnick BJ

更新日期：1973-08-01 00:00:00

abstract：:Clones of embryonic chick chondrocytes have been isolated and collagen biosynthesis has been followed as the clones grow and eventually lose division capacity. Analysis of collagen type at each successive subculture until the time of cellular senescence has shown that a change in synthesis occurs from the cartilage-sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayne R,Vail MS,Mayne PM,Miller EJ

更新日期：1976-05-01 00:00:00

abstract：:A cDNA library prepared from human liver has been screened for factor IX (Christmas factor), a clotting factor that participates in the middle phase of blood coagulation. The library was screened with a single-stranded DNA prepared from enriched mRNA for baboon factor IX and a synthetic oligonucleotide mixture. A plas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kurachi K,Davie EW

更新日期：1982-11-01 00:00:00

abstract：:We have screened a large number of isolated human genomic clones that hybridize to a cloned HLA cDNA probe for their ability to direct the synthesis of HLA-A, -B, and -C surface antigens on mouse L cells following DNA-mediated gene transfer. The surface expression of human histocompatibility antigens, monitored by ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbosa JA,Kamarck ME,Biro PA,Weissman SM,Ruddle FH

更新日期：1982-10-01 00:00:00