Expression profiling of pancreatic beta cells: glucose regulation of secretory and metabolic pathway genes.

abstract：:The interrelationships of hexose feeding and insulin action were studied in the Chinese hamster fibroblast cell lines 023 and DS-7. The latter, derived from 023 and deficient in phosphoglucose isomerase, has been used to map the metabolic requirements for aldohexose-mediated down-regulation or "curbing" of hexose tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson C,Peterson SW,Ullrey DB,Kalckar HM

更新日期：1986-06-01 00:00:00

abstract：:Protein S (ProS) and growth arrest-specific 6 (Gas6) bind to phosphatidylserine (PtdSer) and induce efferocytosis upon binding TAM-family receptors (Tyro3, Axl, and Mer). Here, we produced mouse ProS, Gas6, and TAM-receptor extracellular region fused to IgG fragment crystallizable region in HEK293T cells. ProS and Gas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yanagihashi Y,Segawa K,Maeda R,Nabeshima YI,Nagata S

更新日期：2017-08-15 00:00:00

abstract：:Na(+)-NQR is a unique respiratory enzyme that couples the free energy of electron transfer reactions to electrogenic pumping of sodium across the cell membrane. This enzyme is found in many marine and pathogenic bacteria where it plays an analogous role to the H(+)-pumping complex I. It has generally been assumed that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Juárez O,Morgan JE,Nilges MJ,Barquera B

更新日期：2010-07-13 00:00:00

abstract：:d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roper DI,Huyton T,Vagin A,Dodson G

更新日期：2000-08-01 00:00:00

abstract：:Bone morphogenetic protein 4 (BMP-4) induces ventral mesoderm but represses dorsal mesoderm formation in Xenopus embryos. We show that BMP-4 inhibits two signaling pathways regulating dorsal mesoderm formation, the induction of dorsal mesoderm (Spemann organizer) and the dorsalization of ventral mesoderm. Ectopic expr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Re'em-Kalma Y,Lamb T,Frank D

更新日期：1995-12-19 00:00:00

abstract：:Supernatant fractions (300,000 x g, 60 min) from homogenates of rat liver, heart, and skeletal muscle, dog liver, and rabbit liver prepared without detergent in the homogenization medium (referred to as S300) are shown to contain an activity that restores Mg2+-dependent fluoride- and guanine nucleotide-stimulated cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat MK,Iyengar R,Abramowitz J,Bordelon-Riser ME,Birnbaumer L

更新日期：1980-07-01 00:00:00

abstract：:Proteolytic removal of the pre-segment from growing nascent chains of pre-human placental lactogen (hPL) occurred during in vitro translation of placental mRNA if crude membranes derived from ascites lysates, dog pancreas, or rat liver rough endoplasmic reticulum were added to the translation mixtures. The cotranslati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strauss AW,Zimmerman M,Boime I,Ashe B,Mumford RA,Alberts AW

更新日期：1979-09-01 00:00:00

abstract：:Many and diverse modifications of the myosin subfragment 1 (S-1) increase (modulate) its ATPase activity, including interaction of this particle with actin; a recent addition to these modifications is the extensive lysine modification of S-1 that seems prerequisite to crystallizing it for structure analysis. In this s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bivin DB,Ue K,Khoroshev M,Morales M

更新日期：1994-08-30 00:00:00

abstract：:The anticancer peptide PNC-27, which contains an HDM-2-binding domain corresponding to residues 12-26 of p53 and a transmembrane-penetrating domain, has been found to kill cancer cells (but not normal cells) by inducing membranolysis. We find that our previously determined 3D structure of the p53 residues of PNC-27 is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sarafraz-Yazdi E,Bowne WB,Adler V,Sookraj KA,Wu V,Shteyler V,Patel H,Oxbury W,Brandt-Rauf P,Zenilman ME,Michl J,Pincus MR

更新日期：2010-02-02 00:00:00

abstract：:White matter stroke is a distinct stroke subtype, accounting for up to 25% of stroke and constituting the second leading cause of dementia. The biology of possible tissue repair after white matter stroke has not been determined. In a mouse stroke model, white matter ischemia causes focal damage and adjacent areas of a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sozmen EG,Rosenzweig S,Llorente IL,DiTullio DJ,Machnicki M,Vinters HV,Havton LA,Giger RJ,Hinman JD,Carmichael ST

更新日期：2016-12-27 00:00:00

abstract：:The 1,25-dihydroxyvitamin D3 [1,25-(OH)2vitamin D3] analog KH1060 exerts very potent effects on cell proliferation and cell differentiation via the vitamin D receptor (VDR). However, the activities of KH1060 are not associated with an increased affinity for the VDR. We now show that increased stabilization of the VDR-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van den Bemd GC,Pols HA,Birkenhäger JC,van Leeuwen JP

更新日期：1996-10-01 00:00:00

abstract：:A yeast gene has been identified by screening for DNA replication mutants using a permeabilized cell replication assay. The mutant is temperature sensitive for growth and shows a cell cycle phenotype typical of DNA replication mutants. RNA synthesis is normal in the mutant but DNA synthesis ceases upon shift to the no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Budd ME,Campbell JL

更新日期：1995-08-15 00:00:00

abstract：:A critical step in neuronal development is the formation of axon/dendrite polarity, a process involving symmetry breaking in the newborn neuron. Local self-amplifying processes could enhance and stabilize the initial asymmetry in the distribution of axon/dendrite determinants, but the identity of these processes remai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng PL,Song AH,Wong YH,Wang S,Zhang X,Poo MM

更新日期：2011-11-08 00:00:00

abstract：:We describe a molecular resistance biomarker to gefitinib, epithelial membrane protein-1 (EMP-1). Gefitinib is a small-molecule inhibitor that competes for the ATP-binding site on EGF receptor (EGFR) and has been approved for patients with advanced lung cancers. Treatment with gefitinib has resulted in clinical benefi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jain A,Tindell CA,Laux I,Hunter JB,Curran J,Galkin A,Afar DE,Aronson N,Shak S,Natale RB,Agus DB

更新日期：2005-08-16 00:00:00

abstract：:Accumulating evidence suggests cancer cells exhibit a dependency on metabolic pathways regulated by nicotinamide adenine dinucleotide (NAD+). Nevertheless, how the regulation of this metabolic cofactor interfaces with signal transduction networks remains poorly understood in glioblastoma. Here, we report nicotinamide ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gujar AD,Le S,Mao DD,Dadey DY,Turski A,Sasaki Y,Aum D,Luo J,Dahiya S,Yuan L,Rich KM,Milbrandt J,Hallahan DE,Yano H,Tran DD,Kim AH

更新日期：2016-12-20 00:00:00

abstract：:Methylmalonyl-CoA mutase (2-methylmalonyl-CoA CoA-carbonylmutase, EC 5.4.99.2) is a mitochondrial enzyme whose deficiency in man leads to several biochemically and clinically heterogenous++ forms of methylmalonic acidemia. Intact fibroblasts from 21 patients with mutase apoenzyme deficiency have been pulse-labeled wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fenton WA,Hack AM,Kraus JP,Rosenberg LE

更新日期：1987-03-01 00:00:00

abstract：:We have identified two chromosomal genes of Escherichia coli K12 that are required for the expression of conjugative plasmid functions in the presence of normal plasmid DNA. Hfr cells with mutations in both of these genes are resistant to donor-specific bacteriophage and defective as conjugal donors. These characteris...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McEwen J,Silverman P

更新日期：1980-01-01 00:00:00

abstract：:Binding enhancement by tertiary interactions is a strategy that takes advantage of the higher order folding of functionally important RNAs to bind short nucleic acid-based compounds tightly and more specifically than possible by simple base pairing. For example, tertiary interactions enhance binding of specific hexame...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Testa SM,Gryaznov SM,Turner DH

更新日期：1999-03-16 00:00:00

abstract：:Understanding interactions between membranes requires measurements on well-controlled systems close to natural conditions, in which fluctuations play an important role. We have determined, by grazing incidence X-ray scattering, the interaction potential between two lipid bilayers, one adsorbed on a solid surface and t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hemmerle A,Malaquin L,Charitat T,Lecuyer S,Fragneto G,Daillant J

更新日期：2012-12-04 00:00:00

abstract：:The hypothesis that perceived pain intensity can influence placebo analgesia was tested. One hundred and seven subjects rated their pain from from 0 to 10 on a visual analog scale after a standard wisdom tooth extraction. The expected course of such postoperative pain in the absence of therapy or placebo is a steady i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine JD,Gordon NC,Bornstein JC,Fields HL

更新日期：1979-07-01 00:00:00

abstract：:Investigation of retinal neurochemistry in a well-defined chick model of form-deprivation myopia indicated that dopamine and its metabolite 3,4-dihydroxyphenylacetic acid are reduced in myopic as compared to control eyes. The reduction in retinal dopamine is evident only during light adaptation and is accompanied by a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone RA,Lin T,Laties AM,Iuvone PM

更新日期：1989-01-01 00:00:00

abstract：:Optogenetic methods have been highly effective for suppressing neural activity and modulating behavior in rodents, but effects have been much smaller in primates, which have much larger brains. Here, we present a suite of technologies to use optogenetics effectively in primates and apply these tools to a classic quest...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acker L,Pino EN,Boyden ES,Desimone R

更新日期：2016-11-15 00:00:00

abstract：:The statistics of extrasolar planetary systems indicate that the default mode of planet formation generates planets with orbital periods shorter than 100 days and masses substantially exceeding that of the Earth. When viewed in this context, the Solar System is unusual. Here, we present simulations which show that a p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Batygin K,Laughlin G

更新日期：2015-04-07 00:00:00

abstract：:Extrachromosomal copies of the 1.6-kilobase transposable element Tc1 are present at the level of between 0.1 and 1.0 copy per cell in Caenorhabditis elegans strain Bergerac. Extrachromosomal elements were detected and studied using Southern hybridizations employing a Tc1-specific probe. The amount of extrachromosomal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan K,Emmons SW

更新日期：1984-07-01 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) adopt heterogeneous ensembles of conformations under physiological conditions. Understanding the relationship between amino acid sequence and conformational ensembles of IDPs can help clarify the role of disorder in physiological function. Recent studies revealed that polar IDP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao AH,Crick SL,Vitalis A,Chicoine CL,Pappu RV

更新日期：2010-05-04 00:00:00

abstract：:Because the insolubility of the scrapie prion protein (PrP(Sc)) has frustrated structural studies by x-ray crystallography or NMR spectroscopy, we used electron crystallography to characterize the structure of two infectious variants of the prion protein. Isomorphous two-dimensional crystals of the N-terminally trunca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wille H,Michelitsch MD,Guenebaut V,Supattapone S,Serban A,Cohen FE,Agard DA,Prusiner SB

更新日期：2002-03-19 00:00:00

abstract：:Retroviruses undergo a high frequency of genetic alterations during the process of copying their RNA genomes. However, little is known about the replication fidelity of other elements that transpose via reverse transcription of an RNA intermediate. The complete sequence of 29 independently integrated copies of the yea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gabriel A,Willems M,Mules EH,Boeke JD

更新日期：1996-07-23 00:00:00

abstract：:VIP21/caveolin is localized to both caveolae and apical transport vesicles and presumably cycles between the cell surface and the Golgi complex. We have studied the lipid interactions of this protein by reconstituting Escherichia coli-expressed VIP21/caveolin into liposomes. Surprisingly, the protein reconstituted onl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murata M,Peränen J,Schreiner R,Wieland F,Kurzchalia TV,Simons K

更新日期：1995-10-24 00:00:00

abstract：:We have analyzed the level of intraindividual sequence variability (heteroplasmy) of mtDNA in human brain by denaturing gradient gel electrophoresis and sequencing. Single base substitutions, as well as insertions or deletions of single bases, were numerous in the noncoding control region (D-loop), and 35-45% of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jazin EE,Cavelier L,Eriksson I,Oreland L,Gyllensten U

更新日期：1996-10-29 00:00:00

abstract：:We previously reported that laminar flow activates peroxisome proliferator-activated receptor gamma (PPARgamma) in vascular endothelial cells in a ligand-dependent manner that involves phospholipase A2 and cytochrome P450 epoxygenases. In this study, we investigated whether epoxyeicosatrienoic acids (EETs), the cataly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Y,Zhang Y,Schmelzer K,Lee TS,Fang X,Zhu Y,Spector AA,Gill S,Morisseau C,Hammock BD,Shyy JY

更新日期：2005-11-15 00:00:00