Naturally soluble component(s) that confer(s) guanine nucleotide and fluoride sensitivity to adenylate cyclase.

abstract：:Acute glaucoma is a sight-threatening condition characterized by a sudden and substantial rise in intraocular pressure (IOP) and consequent retinal ganglion cell (RGC) death. Angle closure glaucoma, a common cause of glaucoma in Asia that affects tens of millions of people worldwide, often presents acutely with loss o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chi W,Li F,Chen H,Wang Y,Zhu Y,Yang X,Zhu J,Wu F,Ouyang H,Ge J,Weinreb RN,Zhang K,Zhuo Y

更新日期：2014-07-29 00:00:00

abstract：:A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaptain S,Downey WE,Tang C,Philpott C,Haile D,Orloff DG,Harford JB,Rouault TA,Klausner RD

更新日期：1991-11-15 00:00:00

abstract：:Acute and chronic injuries to the heart result in perturbation of intracellular calcium signaling, which leads to pathological cardiac hypertrophy and remodeling. Calcium/calmodulin-dependent protein kinase II (CaMKII) has been implicated in the transduction of calcium signals in the heart, but the specific isoforms o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Backs J,Backs T,Neef S,Kreusser MM,Lehmann LH,Patrick DM,Grueter CE,Qi X,Richardson JA,Hill JA,Katus HA,Bassel-Duby R,Maier LS,Olson EN

更新日期：2009-02-17 00:00:00

abstract：:Recently, hepatic peroxisome proliferator-activated receptor (PPAR)γ has been implicated in hepatic lipid accumulation. We found that the C3H mouse strain does not express PPARγ in the liver and, when subject to a high-fat diet, is resistant to hepatic steatosis, compared with C57BL/6 (B6) mice. Adenoviral PPARγ2 inje...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee YJ,Ko EH,Kim JE,Kim E,Lee H,Choi H,Yu JH,Kim HJ,Seong JK,Kim KS,Kim JW

更新日期：2012-08-21 00:00:00

abstract：:Under steady-state conditions, the immune system is poised to sense and respond to the microbiota. As such, immunity to the microbiota, including T cell responses, is expected to precede any inflammatory trigger. How this pool of preformed microbiota-specific T cells contributes to tissue pathologies remains unclear. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hurabielle C,Link VM,Bouladoux N,Han SJ,Merrill ED,Lightfoot YL,Seto N,Bleck CKE,Smelkinson M,Harrison OJ,Linehan JL,Tamoutounour S,Lionakis MS,Kaplan MJ,Nakajima S,Belkaid Y

更新日期：2020-07-14 00:00:00

abstract：:The Japanese firefly squid, Watasenia scintillans, emits intense flashes of light from three tiny luminous organs that are located at the tip of each of a pair of ventral arms. Light is also produced from hundreds of other minute organs that are scattered over the body. The luminescence is due to an ATP-dependent reac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsuji FI

更新日期：1985-07-01 00:00:00

abstract：:Biological membranes are complex, self-organized structures that define boundaries and compartmentalize space in living matter. Composed of a wide variety of lipid and protein molecules, these responsive surfaces mediate transmembrane signaling and material transport within the cell and with its environment. It is wel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watkins EB,Miller CE,Majewski J,Kuhl TL

更新日期：2011-04-26 00:00:00

abstract：:We investigate the dynamics and spatial distribution of land use fragmentation in a rapidly urbanizing region of the United States to test key propositions regarding the evolution of sprawl. Using selected pattern metrics and data from 1973 and 2000 for the state of Maryland, we find significant increases in developed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irwin EG,Bockstael NE

更新日期：2007-12-26 00:00:00

abstract：:When a local mechanical perturbation is applied to the surface of a thin film of a mechanically interlocked molecule (a rotaxane), the molecules self-organize into periodic arrays of discrete dots or lines. The dimensionality of the nanostructures depends on whether the mechanical stimulus acts along a 1D line or over...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biscarini F,Cavallini M,Kshirsagar R,Bottari G,Leigh DA,León S,Zerbetto F

更新日期：2006-11-21 00:00:00

abstract：:For the emergence of early life, the formation of biopolymers such as RNA is essential. However, the addition of nucleotide monomers to existing oligonucleotides requires millimolar concentrations. Even in such optimistic settings, no polymerization of RNA longer than about 20 bases could be demonstrated. How then cou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mast CB,Schink S,Gerland U,Braun D

更新日期：2013-05-14 00:00:00

abstract：:Flowering time (i.e., heading date in crops) is an important ecological trait that determines growing seasons and regional adaptability of plants to specific natural environments. Rice (Oryza sativa L.) is a short-day plant that originated in the tropics. Increasing evidence suggests that the northward expansion of cu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu W,Zheng XM,Lu G,Zhong Z,Gao H,Chen L,Wu C,Wang HJ,Wang Q,Zhou K,Wang JL,Wu F,Zhang X,Guo X,Cheng Z,Lei C,Lin Q,Jiang L,Wang H,Ge S,Wan J

更新日期：2013-02-19 00:00:00

abstract：:The serum thymic factor (FTS) utilized in its synthetic or natural form loses its biological activity in a rosette assay after treatment with a metal ion-chelating agent, Chelex 100. This activity is restored by the addition of Zn salts and, to a lesser extent, certain other metal salts. FTS activation is secondary to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dardenne M,Pléau JM,Nabarra B,Lefrancier P,Derrien M,Choay J,Bach JF

更新日期：1982-09-01 00:00:00

abstract：:Pin1 is a modular enzyme that accelerates the cis-trans isomerization of phosphorylated-Ser/Thr-Pro (pS/T-P) motifs found in numerous signaling proteins regulating cell growth and neuronal survival. We have used NMR to investigate the interaction of Pin1 with three related ligands that include a pS-P substrate peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Namanja AT,Wang XJ,Xu B,Mercedes-Camacho AY,Wilson KA,Etzkorn FA,Peng JW

更新日期：2011-07-26 00:00:00

abstract：:A protein of 87 kilodaltons (87 kDa) was previously identified as a major specific substrate for protein kinase C in neuronal and other tissues. We have now studied the effect of protein kinase C-catalyzed phosphorylation of this protein on its association with membranes in isolated nerve terminals (synaptosomes) from...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang JK,Walaas SI,Sihra TS,Aderem A,Greengard P

更新日期：1989-04-01 00:00:00

abstract：:In many song birds, males develop their songs as adults by imitating the songs of one or more tutors, memorized previously during a sensitive phase early in life. Previous work using two assays, the production of imitations by adult males and playback-induced calling by young birds during the sensitive phase for memor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nelson DA

更新日期：2000-11-21 00:00:00

abstract：:An homologous in vitro assay for yeast nonsense suppressors was used to examine the effect of the cytoplasmically inherited genetic determinant [psi] on the efficiency of in vitro nonsense suppression. The efficiency of all three types of yeast tRNA-mediated nonsense suppressor (ochre, amber, and UGA) is much greater ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tuite MF,Cox BS,McLaughlin CS

更新日期：1983-05-01 00:00:00

abstract：:The human tumor necrosis factor-alpha (TNF-alpha) gene encodes a pleiotropic cytokine that plays a critical role in basic immunologic processes. To investigate the TNF-alpha regulatory region in the primate lineage, we isolated TNF-alpha promoters from representative great apes, Old World monkeys, and New World monkey...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung JY,McKenzie FE,Uglialoro AM,Flores-Villanueva PO,Sorkin BC,Yunis EJ,Hartl DL,Goldfeld AE

更新日期：2000-06-06 00:00:00

abstract：:Activation of androgen receptor (AR) via androgen in muscle cells has been closely linked to their growth and differentiation. Here, we report the cloning and characterization of supervillin (SV), a 205-kDa actin-binding protein, as an AR coregulator from the skeletal muscle cDNA library. Mammalian two-hybrid and glut...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ting HJ,Yeh S,Nishimura K,Chang C

更新日期：2002-01-22 00:00:00

abstract：:We report a molecular analysis of the chicken Ig loci in single bursal follicles from 3- to 7-week-old chickens. Each follicle contained between 10(5) and 3 X 10(5) cells. The Ig gene rearrangement patterns obtained were compared to the pattern observed with the corresponding total bursal DNA. The results obtained for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weill JC,Reynaud CA,Lassila O,Pink JR

更新日期：1986-05-01 00:00:00

abstract：:Mutagenic specificity of 2-acetylaminofluorene (AAF) has been established in mammalian cells and several strains of bacteria by using a shuttle plasmid vector containing a single N-(deoxyguanosin-8-yl)acetylaminofluorene (C8-dG-AAF) adduct. The nucleotide sequence of the gene conferring tetracycline resistance was mod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moriya M,Takeshita M,Johnson F,Peden K,Will S,Grollman AP

更新日期：1988-03-01 00:00:00

abstract：:We demonstrate how a conventional confocal spinning-disk (CSD) microscope can be converted into a doubly resolving image scanning microscopy (ISM) system without changing any part of its optical or mechanical elements. Making use of the intrinsic properties of a CSD microscope, we illuminate stroboscopically, generati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz O,Pieper C,Clever M,Pfaff J,Ruhlandt A,Kehlenbach RH,Wouters FS,Großhans J,Bunt G,Enderlein J

更新日期：2013-12-24 00:00:00

abstract：:Lithium-air batteries have captured worldwide attention due to their highest energy density among the chemical batteries. To provide continuous oxygen channels, here, we synthesized hierarchical mesoporous perovskite La(0.5)Sr(0.5)CoO(2.91) (LSCO) nanowires. We tested the intrinsic oxygen reduction reaction (ORR) and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Y,Xu L,Mai L,Han C,An Q,Xu X,Liu X,Zhang Q

更新日期：2012-11-27 00:00:00

abstract：:Phosducin is a soluble phosphoprotein found in retinal photoreceptor cells and in the pineal gland. It binds to the beta gamma subunits of guanine nucleotide-binding proteins (G proteins) (G beta gamma) and may regulate G-protein function. In this study, the ability of specific regions of phosducin to bind G beta gamm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Wu D,Slepak VZ,Simon MI

更新日期：1995-03-14 00:00:00

abstract：:Preterm birth (PTB) complications are the leading cause of long-term morbidity and mortality in children. By using whole blood samples, we integrated whole-genome sequencing (WGS), RNA sequencing (RNA-seq), and DNA methylation data for 270 PTB and 521 control families. We analyzed this combined dataset to identify gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knijnenburg TA,Vockley JG,Chambwe N,Gibbs DL,Humphries C,Huddleston KC,Klein E,Kothiyal P,Tasseff R,Dhankani V,Bodian DL,Wong WSW,Glusman G,Mauldin DE,Miller M,Slagel J,Elasady S,Roach JC,Kramer R,Leinonen K,Linth

更新日期：2019-03-19 00:00:00

abstract：:Immunoprecipitation of proteins extracted from metabolically labeled human glioblastoma and fibrosarcoma cells with antiserum to platelet-derived growth factor (PDGF) showed that these cells express and secrete proteins that are recognized specifically by the antiserum. The molecular masses of immunoprecipitated prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis P,Pelicci PG,Dalla-Favera R,Antoniades HN

更新日期：1985-04-01 00:00:00

abstract：:Alterations in the activity/levels of the extralarge G protein α-subunit (XLαs) are implicated in various human disorders, such as perinatal growth retardation. Encoded by GNAS, XLαs is partly identical to the α-subunit of the stimulatory G protein (Gsα), but the cellular actions of XLαs remain poorly defined. Followi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He Q,Bouley R,Liu Z,Wein MN,Zhu Y,Spatz JM,Wang CY,Divieti Pajevic P,Plagge A,Babitt JL,Bastepe M

更新日期：2017-11-07 00:00:00

abstract：:The structure of respiratory cytochrome c551 of Pseudomonas aeruginosa, with 82 amino acids, has been solved by x-ray analysis and refined to a crystallographic R factor of 16.2%. It has the same basic folding pattern and hydrophobic heme environment as cytochromes c, c2, and c550, except for a large deletion at the b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Almassy RJ,Dickerson RE

更新日期：1978-06-01 00:00:00

abstract：:Scientific cinematography using ultrafast optical imaging is a common tool to study motion. In opaque organisms or structures, X-ray radiography captures sequences of 2D projections to visualize morphological dynamics, but for many applications full four-dimensional (4D) spatiotemporal information is highly desirable....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： dos Santos Rolo T,Ershov A,van de Kamp T,Baumbach T

更新日期：2014-03-18 00:00:00

abstract：:The human Melan-A/MART-1 gene encodes an HLA-A2-restricted peptide epitope recognized by melanoma-reactive CD8(+) cytotoxic T lymphocytes. Here we report that this gene also encodes at least one HLA-DR4-presented peptide recognized by CD4(+) T cells. The Melan-A/MART-1(51-73) peptide was able to induce the in vitro ex...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zarour HM,Kirkwood JM,Kierstead LS,Herr W,Brusic V,Slingluff CL Jr,Sidney J,Sette A,Storkus WJ

更新日期：2000-01-04 00:00:00

abstract：:A rapid method for quantitating products of the oligodeoxynucleotide processing reaction in vitro has been developed to facilitate enzymatic studies of the retroviral integrases. Unlike earlier procedures, this assay does not depend on polyacrylamide gel electrphoresis but separates products by batch adsorption to PEI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller B,Jones KS,Merkel GW,Skalka AM

更新日期：1993-12-15 00:00:00