A regulated RNA binding protein also possesses aconitase activity.

Abstract:

:A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.

authors

Kaptain S,Downey WE,Tang C,Philpott C,Haile D,Orloff DG,Harford JB,Rouault TA,Klausner RD

doi

10.1073/pnas.88.22.10109

subject

Has Abstract

pub_date

1991-11-15 00:00:00

pages

10109-13

issue

22

eissn

0027-8424

issn

1091-6490

journal_volume

88

pub_type

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