Abstract:
:An homologous in vitro assay for yeast nonsense suppressors was used to examine the effect of the cytoplasmically inherited genetic determinant [psi] on the efficiency of in vitro nonsense suppression. The efficiency of all three types of yeast tRNA-mediated nonsense suppressor (ochre, amber, and UGA) is much greater in cell-free lysates prepared from a sup+ [psi+] strain than in lysates prepared from an isogeneic sup+ [psi-] strain. Lysates prepared from a [psi-] strain, into which the [psi+] determinant was reintroduced by kar1-mediated cytoduction, support efficient suppression. Evidence is also presented that [psi-] lysates contain an inhibitor of in vitro nonsense suppression.
journal_name
Proc Natl Acad Sci U S Aauthors
Tuite MF,Cox BS,McLaughlin CSdoi
10.1073/pnas.80.10.2824subject
Has Abstractpub_date
1983-05-01 00:00:00pages
2824-8issue
10eissn
0027-8424issn
1091-6490journal_volume
80pub_type
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