Abstract:
:d-alanine-d-lactate ligase from Enterococcus faecium BM4147 is directly responsible for the biosynthesis of alternate cell-wall precursors in bacteria, which are resistant to the glycopeptide antibiotic vancomycin. The crystal structure has been determined with data extending to 2.5-A resolution. This structure shows that the active site has unexpected interactions and is distinct from previous models for d-alanyl-d-lactate ligase mechanistic studies. It appears that the preference of the enzyme for lactate as a ligand over d-alanine could be mediated by electrostatic effects and/or a hydrogen-bonding network, which principally involve His-244. The structure of d-alanyl-d-lactate ligase provides a revised interpretation of the molecular events that lead to vancomycin resistance.
journal_name
Proc Natl Acad Sci U S Aauthors
Roper DI,Huyton T,Vagin A,Dodson Gdoi
10.1073/pnas.150116497keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
8921-5issue
16eissn
0027-8424issn
1091-6490pii
150116497journal_volume
97pub_type
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