Abstract:
:A mixture of two recombinant plasmids was microinjected into mouse thymidine kinase-negative fibroblasts (L cells). One plasmid contained the thymidine kinase gene of herpes simplex virus type I and the other contained the human beta globin gene. Seven fibroblast colonies arising from injected cells incubated in hypoxanthine/aminopterin/thymidine medium were analyzed. These microinjected cells were shown to: (i) produce functionally active herpes simplex type I thymidine kinase enzyme, (ii) replicate the human beta globin gene, and (iii) produce human beta globin mRNA sequences at low levels. Thus, the genetic defect (lack of thymidine kinase activity) was corrected by the microinjected thymidine kinase gene, and a coinjected human beta globin gene was replicated and weakly expressed.
journal_name
Proc Natl Acad Sci U S Aauthors
Anderson WF,Killos L,Sanders-Haigh L,Kretschmer PJ,Diacumakos EGdoi
10.1073/pnas.77.9.5399subject
Has Abstractpub_date
1980-09-01 00:00:00pages
5399-403issue
9eissn
0027-8424issn
1091-6490journal_volume
77pub_type
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更新日期:2020-05-26 00:00:00