Permanence or change? The meaning of genetic variation.

abstract：:In genotypic mutation analysis DNA sequence changes are determined without the in vivo or in vitro selection of phenotypically altered cells. We have studied the induction of base-pair changes by N-ethyl-N-nitrosourea in Taq I endonuclease recognition site 2508-2511 (TCGA) of the c-H-ras1 gene in human fibroblasts by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiocca SM,Sandy MS,Cerutti PA

更新日期：1992-06-15 00:00:00

abstract：:In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Aalten DM,Synstad B,Brurberg MB,Hough E,Riise BW,Eijsink VG,Wierenga RK

更新日期：2000-05-23 00:00:00

abstract：:A method has been developed for de novo peptide sequencing using matrix-assisted laser desorption ionization mass spectrometry. This method will facilitate biological studies that require rapid determination of peptide or protein sequences, e.g., determination of posttranslational modifications, identification of acti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keough T,Youngquist RS,Lacey MP

更新日期：1999-06-22 00:00:00

abstract：:The product of the Nijmegen breakage syndrome gene (NBS1) plays crucial roles in DNA damage response through its association with many proteins, including MRE11 and RAD50. However, it remains to be determined exactly how NBS1 accumulates at or near DNA double-strand breaks. Here we report that MDC1 directly binds to N...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu L,Luo K,Lou Z,Chen J

更新日期：2008-08-12 00:00:00

abstract：:In this report we identify the specific isozymes of protein kinase C (PKC) that are involved in c-fos and c-jun mRNA accumulation in the rat basophilic leukemia cell line RBL-2H3. These cells could be largely depleted of the endogenous PKC isozymes by chronic treatment with phorbol 12-myristate 13-acetate followed by ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razin E,Szallasi Z,Kazanietz MG,Blumberg PM,Rivera J

更新日期：1994-08-02 00:00:00

abstract：:The mammalian target of rapamycin (mTOR) is a central regulator of cell growth and proliferation in response to growth factor and nutrient signaling. Consequently, this kinase is implicated in metabolic diseases including cancer and diabetes, so there is great interest in understanding the complete spectrum of mTOR-re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang BT,Ducker GS,Barczak AJ,Barbeau R,Erle DJ,Shokat KM

更新日期：2011-09-13 00:00:00

abstract：:We have carried out partial amino acid sequence analysis of a putative nuclear pore complex protein (nucleoporin) of rat that reacts with wheat germ agglutinin and with the polyspecific monoclonal antibody 414. Surprisingly, these partial amino acid sequence data revealed a high degree of similarity with the human CAN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kraemer D,Wozniak RW,Blobel G,Radu A

更新日期：1994-02-15 00:00:00

abstract：:Fumarase C was strongly induced by paraquat in a parental strain of Escherichia coli but was not induced in a strain lacking the soxRS response. Moreover, a strain that constitutively expresses the soxRS regulon contained more fumarase C than did the parental strain. The Mn-containing superoxide dismutase and glucose-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liochev SI,Fridovich I

更新日期：1992-07-01 00:00:00

abstract：:During early postnatal development, sensory regions of the brain undergo periods of heightened plasticity which sculpt neural networks and lay the foundation for adult sensory perception. Such critical periods were also postulated for learning and memory but remain elusive and poorly understood. Here, we present evide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao X,Castro-Gomez S,Grendel J,Graf S,Süsens U,Binkle L,Mensching D,Isbrandt D,Kuhl D,Ohana O

更新日期：2018-12-04 00:00:00

abstract：:We have examined the functional consequences of mutations present in defective alleles of the retinoblastoma susceptibility gene (RB1) isolated from two spontaneously arising tumors. Unlike cDNA clones expressing the wild-type protein p110Rb, those encoding the two mutant proteins failed to induce the appearance of se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Templeton DJ,Park SH,Lanier L,Weinberg RA

更新日期：1991-04-15 00:00:00

abstract：:The L-A double-stranded RNA virus of Saccharomyces cerevisiae makes a gag-pol fusion protein by a -1 ribosomal frameshift. The pol amino acid sequence includes consensus patterns typical of the RNA-dependent RNA polymerases (EC 2.7.7.48) of (+) strand and double-stranded RNA viruses of animals and plants. We have carr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ribas JC,Wickner RB

更新日期：1992-03-15 00:00:00

abstract：:Multiple sclerosis is a chronic disease of the central nervous system (CNS) characterized by inflammation, demyelination, and axonal loss. The immunopathogenesis of demyelination in multiple sclerosis involves an autoantibody response to myelin oligodendrocyte glycoprotein (MOG), a type I transmembrane protein located...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breithaupt C,Schubart A,Zander H,Skerra A,Huber R,Linington C,Jacob U

更新日期：2003-08-05 00:00:00

abstract：:Prostate cancer is a leading cause of cancer death in men over 50 years of age, and there is a characteristic marked decrease in Zn content in the malignant prostate cells. The cause and consequences of this loss have thus far been unknown. We found that in middle-aged rats a Zn-deficient diet reduces prostatic Zn lev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fong LY,Jing R,Smalley KJ,Wang ZX,Taccioli C,Fan S,Chen H,Alder H,Huebner K,Farber JL,Fiehn O,Croce CM

更新日期：2018-11-20 00:00:00

abstract：:Infection of HeLa cells with poliovirus results in an inhibition of host-cell protein synthesis. Cytoplasmic extracts from infected cells contian an activity that inhibits initiation of protein synthesis in vitro by rabbit reticulocyte lysates. This inhibitory activity has been purified and characterized, and is shown...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ehrenfeld E,Hunt T

更新日期：1971-05-01 00:00:00

abstract：:In preeclampsia (PE), cytotrophoblast (CTB) invasion of the uterus and spiral arteries is often shallow. Thus, the placenta's role has been a focus. In this study, we tested the hypothesis that decidual defects are an important determinant of the placental phenotype. We isolated human endometrial stromal cells from no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garrido-Gomez T,Dominguez F,Quiñonero A,Diaz-Gimeno P,Kapidzic M,Gormley M,Ona K,Padilla-Iserte P,McMaster M,Genbacev O,Perales A,Fisher SJ,Simón C

更新日期：2017-10-03 00:00:00

abstract：:XMAP215/Dis1 family proteins positively regulate microtubule growth. Repeats at their N termini, called TOG domains, are important for this function. While TOG domains directly bind tubulin dimers, it is unclear how this interaction translates to polymerase activity. Understanding the functional roles of TOG domains i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Widlund PO,Stear JH,Pozniakovsky A,Zanic M,Reber S,Brouhard GJ,Hyman AA,Howard J

更新日期：2011-02-15 00:00:00

abstract：:The chemokine stromal cell-derived factor 1, SDF-1, is an important regulator of leukocyte and hematopoietic precursor migration and pre-B cell proliferation. The receptor for SDF-1, CXCR4, also functions as a coreceptor for T-tropic HIV-1 entry. We find that mice deficient for CXCR4 die perinatally and display profou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma Q,Jones D,Borghesani PR,Segal RA,Nagasawa T,Kishimoto T,Bronson RT,Springer TA

更新日期：1998-08-04 00:00:00

abstract：:The nematode Caenorhabditis elegans exhibits behavioral responses to many volatile odorants. Chemotaxis toward one such odorant, diacetyl (butanedione), requires the function of a seven-transmembrane receptor protein encoded by the odr-10 gene. To determine directly whether ODR-10 protein is an odorant receptor, it is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Chou JH,Bradley J,Bargmann CI,Zinn K

更新日期：1997-10-28 00:00:00

abstract：:Maintaining a fleet of buses to transport students to school is a major expense for school districts. To reduce costs by reusing buses between schools, many districts spread start times across the morning. However, assigning each school a time involves estimating the impact on transportation costs and reconciling addi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bertsimas D,Delarue A,Martin S

更新日期：2019-03-26 00:00:00

abstract：:Four monoclonal anti-parathyroid antibodies were generated after immunization of mice with intact cells from human parathyroid tissue. All four monoclonal antibodies reacted in immunohistochemistry with structures present on parathyroid epithelial cells and proximal-tubule cells of the kidney but were unreactive with ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Juhlin C,Holmdahl R,Johansson H,Rastad J,Akerström G,Klareskog L

更新日期：1987-05-01 00:00:00

abstract：:Two decades after the discovery of the first animal microRNA (miRNA), the number of miRNAs in animal genomes remains a vexing question. Here, we report findings from analyzing 1,323 short RNA sequencing samples (RNA-seq) from 13 different human tissue types. Using stringent thresholding criteria, we identified 3,707 s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Londin E,Loher P,Telonis AG,Quann K,Clark P,Jing Y,Hatzimichael E,Kirino Y,Honda S,Lally M,Ramratnam B,Comstock CE,Knudsen KE,Gomella L,Spaeth GL,Hark L,Katz LJ,Witkiewicz A,Rostami A,Jimenez SA,Hollingsworth MA,

更新日期：2015-03-10 00:00:00

abstract：:Increased cardiovascular mortality occurs in diabetic patients with or without coronary artery disease and is attributed to the presence of diabetic cardiomyopathy. One potential mechanism is hyperglycemia that has been reported to activate protein kinase C (PKC), preferentially the beta isoform, which has been associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakasaki H,Koya D,Schoen FJ,Jirousek MR,Ways DK,Hoit BD,Walsh RA,King GL

更新日期：1997-08-19 00:00:00

abstract：:The molecular mechanism of desensitization of soluble guanylyl cyclase (sGC), the NO receptor, has long remained unresolved. Posttranslational modification and redox state have been postulated to affect sGC sensitivity to NO but evidence has been lacking. We now show that sGC can be S-nitrosylated in primary aortic sm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sayed N,Baskaran P,Ma X,van den Akker F,Beuve A

更新日期：2007-07-24 00:00:00

abstract：:An assay has been developed to test the hypothesis that neurons from a limited area of the retina will adhere preferentially to that part of the optic tectum near their normal synaptic termini. The method measures the adherence of (32)P-labeled cell bodies from either the dorsal or ventral half of the neural retina of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbera AJ,Marchase RB,Roth S

更新日期：1973-09-01 00:00:00

abstract：:Arc repressor forms a homodimer in which the subunits intertwine to create a single globular domain. To obtain Arc sequences that fold preferentially as heterodimers, variants with surface patches of excess positive or negative charge were designed. Several but not all oppositely charged sequence pairs showed preferen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nohaile MJ,Hendsch ZS,Tidor B,Sauer RT

更新日期：2001-03-13 00:00:00

abstract：:Protein-protein interactions allow the retinoid X receptor (RXR) to bind to cognate DNA as a homo- or a heterodimer and to participate in mediating the effects of a variety of hormones on gene transcription. Here we report a systematic study of the oligomeric state of RXR in the absence of a DNA template. We have used...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kersten S,Kelleher D,Chambon P,Gronemeyer H,Noy N

更新日期：1995-09-12 00:00:00

abstract：:Recent experiments have shown that mouse primary visual cortex (V1) is very different from that of cat or monkey, including response properties-one of which is that contrast invariance in the orientation selectivity (OS) of the neurons' firing rates is replaced in mouse with contrast-dependent sharpening (broadening) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai WP,Zhou D,McLaughlin DW,Cai D

更新日期：2018-11-06 00:00:00

abstract：:DNA topoisomerase I (top1) is the target of potent anticancer agents, including camptothecins and DNA intercalators, which reversibly stabilize (trap) top1 catalytic intermediates (cleavage complexes). The aim of the present study was to define the structural relationship between the site(s) of covalently bound interc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pommier Y,Laco GS,Kohlhagen G,Sayer JM,Kroth H,Jerina DM

更新日期：2000-09-26 00:00:00

abstract：:The expression of a number of genes encoding products involved in copper ion uptake in yeast is specifically inhibited by copper ions. We show here that copper metalloregulation occurs through Cu-dependent repression of the transactivation activity of Mac1p. A segment of the yeast transcription factor Mac1p was identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graden JA,Winge DR

更新日期：1997-05-27 00:00:00

abstract：:We have cloned an additional member (GC-D) of the membrane receptor guanylyl cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] family that is specifically expressed in a subpopulation of olfactory sensory neurons. The extracellular, putative ligand-binding domain of the olfactory cyclase is similar in primary ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fülle HJ,Vassar R,Foster DC,Yang RB,Axel R,Garbers DL

更新日期：1995-04-11 00:00:00