The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm.

abstract：:The kinetics for hydrogen exchange at individual base pairs in self-complementary deoxydodecanucleotide duplexes have been estimated from NMR saturation recovery measurements on the resolved imino protons as a function of temperature. The imino protons of dA . dT base pairs in the center of the fully alternating d(C-G...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel DJ,Ikuta S,Kozlowski S,Itakura K

更新日期：1983-04-01 00:00:00

abstract：:Low-dimensional objects such as molecular strands, ladders, and sheets have intrinsic features that affect their propensity to fold into 3D objects. Understanding this relationship remains a challenge for de novo design of functional structures. Using molecular dynamics simulations, we investigate the refolding of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dodd PM,Damasceno PF,Glotzer SC

更新日期：2018-07-17 00:00:00

abstract：:Dimension reduction methods are commonly applied to high-throughput biological datasets. However, the results can be hindered by confounding factors, either biological or technical in origin. In this study, we extend principal component analysis (PCA) to propose AC-PCA for simultaneous dimension reduction and adjustme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin Z,Yang C,Zhu Y,Duchi J,Fu Y,Wang Y,Jiang B,Zamanighomi M,Xu X,Li M,Sestan N,Zhao H,Wong WH

更新日期：2016-12-20 00:00:00

abstract：:Helping and sharing among humans is often motivated by empathy and accompanied by a sense of satisfaction. To determine whether similar self-rewarding mechanisms may underpin assistance among nonhuman primates, eight female brown capuchin monkeys (Cebus apella) underwent testing in a simple choice paradigm. Paired wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Waal FB,Leimgruber K,Greenberg AR

更新日期：2008-09-09 00:00:00

abstract：:neurogenin2 (ngn2) encodes a basic helix-loop-helix transcription factor and plays an important role in neurogenesis from migratory neural crest cells. Its role in retinal development is poorly understood. We observed that in the developing chick retina, ngn2 was expressed in a subpopulation of proliferating progenito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan RT,Ma WX,Wang SZ

更新日期：2001-12-18 00:00:00

abstract：:Light microscopy autoradiography has been used to visualize neuroanatomical patterns of brain opiate receptor upregulation in response to chronic naltrexone administration. Slide-mounted brain sections of frozen rat brain were labeled in vitro with dihydro[3H]morphine, a relatively selective mu opioid ligand. The grea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tempel A,Gardner EL,Zukin RS

更新日期：1984-06-01 00:00:00

abstract：:Hepatocyte growth factor (HGF) is an activating ligand of the Met receptor tyrosine kinase, whose activity is essential for normal tissue development and organ regeneration but abnormal activation of Met has been implicated in growth, invasion, and metastasis of many types of solid tumors. HGF has two natural splice v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tolbert WD,Daugherty-Holtrop J,Gherardi E,Vande Woude G,Xu HE

更新日期：2010-07-27 00:00:00

abstract：:Insulin activates sterol regulatory element-binding protein-1c (SREBP-1c) in liver, thereby increasing fatty acid and triglyceride synthesis. We created a line of transgenic rats that produce epitope-tagged human SREBP-1c in liver under control of the constitutive apolipoprotein E promoter/enhancer. This system allows...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Owen JL,Zhang Y,Bae SH,Farooqi MS,Liang G,Hammer RE,Goldstein JL,Brown MS

更新日期：2012-10-02 00:00:00

abstract：:This investigation was undertaken to determine the possible role of growth hormone (GH) in the hormonal regulation of hepatic low density lipoprotein (LDL) receptor expression. Treatment of normal rats with estrogen (ethynylestradiol, 5 mg/kg per day) increased the number of hepatic LDL receptors, and the LDL receptor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rudling M,Norstedt G,Olivecrona H,Reihnér E,Gustafsson JA,Angelin B

更新日期：1992-08-01 00:00:00

abstract：:Norharman enhanced the known mutagenicity of 2-acetylaminofluorene derivatives in the Salmonella test system. The mutagenicities of 2-acetylaminofluorene, 2-aminofluorene, and N-hydroxy-2-acetylaminofluorene were enhanced by norharman only when rat liver microsomal enzymes were added, whereas the mutagenicity of N-ace...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Umezawa K,Shirai A,Matsushima T,Sugimura T

更新日期：1978-02-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNF-alpha) stimulated the phosphorylation of a 28-kDa protein (p28) in the ME-180 line of human cervical carcinoma cells. The effect of TNF-alpha on the phosphorylation state of p28 was rapid (4-fold increase within 15 min) and persistent, remaining above the basal level for at least 2 hr....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marino MW,Pfeffer LM,Guidon PT Jr,Donner DB

更新日期：1989-11-01 00:00:00

abstract：:The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ungewickell A,Ward ME,Ungewickell E,Majerus PW

更新日期：2004-09-14 00:00:00

abstract：:Our anatomical analysis revealed that a dry maize seed contains four to five embryonic leaves at different developmental stages. Rudimentary kranz structure (KS) is apparent in the first leaf with a substantial density, but its density decreases toward younger leaves. Upon imbibition, leaf expansion occurs rapidly wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu WY,Chang YM,Chen SC,Lu CH,Wu YH,Lu MY,Chen DR,Shih AC,Sheue CR,Huang HC,Yu CP,Lin HH,Shiu SH,Ku MS,Li WH

更新日期：2013-03-05 00:00:00

abstract：:The three-dimensional crystal structure of variant-3 toxin from the scorpion Centruroides sculpturatus Ewing has been determined at 3 A resolution. Phases were obtained by use of K2PtCl4 and K2IrCl6 derivatives. The most prominent secondary structural features are two and a half turns of alpha-helix and a three-strand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fontecilla-Camps JC,Almassy RJ,Suddath FL,Watt DD,Bugg CE

更新日期：1980-11-01 00:00:00

abstract：:von Willebrand factor (vWF) is a multimeric glycoprotein that mediates the adhesion of platelets to the subendothelium by binding to platelet glycoprotein Ib. For human vWF, this interaction can be induced in vitro by the antibiotic ristocetin or the snake venom protein botrocetin. A missense mutation, Gly-561-->Ser, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rabinowitz I,Tuley EA,Mancuso DJ,Randi AM,Firkin BG,Howard MA,Sadler JE

更新日期：1992-10-15 00:00:00

abstract：:Structural information about rat enteroglucagon, intestinal peptides containing the pancreatic glucagon sequence, has been based previously on cDNA, immunologic, and chromatographic data. Our interests in testing the physiological actions of synthetic enteroglucagon peptides in rats required that we identify precisely...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collie NL,Walsh JH,Wong HC,Shively JE,Davis MT,Lee TD,Reeve JR Jr

更新日期：1994-09-27 00:00:00

abstract：:Proalbumins are rare genetic variants of human serum albumin containing a basic propeptide that is not removed during post-transcriptional processing because of a mutation in the site of excision, an Arg-Arg sequence. We have identified the amino acid substitutions in three different types of proalbumins designated Ga...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takahashi N,Takahashi Y,Putnam FW

更新日期：1987-11-01 00:00:00

abstract：:The distribution of enkephalin-like immunoreactivity within the avian retina was studied by immunofluorescence and immunoperoxidase techniques with antiserum to [Met5]enkephalin and [Leu5]enkephalin. Formaldehyde-fixed retinae were sectioned and incubated in antiserum to either [Met5]- or [Leu5]enkephalin. Specificity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brecha N,Karten HJ,Laverack C

更新日期：1979-06-01 00:00:00

abstract：:Genome editing with engineered site-specific endonucleases involves nonhomologous end-joining, leading to reading frame disruption. The approach is applicable to dominant negative disorders, which can be treated simply by knocking out the mutant allele, while leaving the normal allele intact. We applied this strategy ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shinkuma S,Guo Z,Christiano AM

更新日期：2016-05-17 00:00:00

abstract：:L-Asparaginase (EC 3.5.1.1) inhibited respiration in sensitive, but not resistant, lines of murine lymphoma 6C3HED. Glucose, in these tumor lines, was principally converted to lactate, and very little was oxidized in the citric acid cycle or hexose monophosphate shunt. The cells derived 70-80% of their respiratory CO(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lavietes BB,Regan DH,Demopoulos HB

更新日期：1974-10-01 00:00:00

abstract：:The signal recognition particle (SRP) is a ribonucleoprotein composed of an Alu domain and an S domain. The S domain contains unique sequence SRP RNA and four SRP proteins: SRP19, SRP54, SRP68, and SRP72. SRP interacts with ribosomes to bring translating membrane and secreted proteins to the endoplasmic reticulum (ER)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Politz JC,Yarovoi S,Kilroy SM,Gowda K,Zwieb C,Pederson T

更新日期：2000-01-04 00:00:00

abstract：:The fluorescence decay of the rare earth terbium when bound to the protein calmodulin changes from a simple exponential decay to a complex nonexponential decay as the temperature is lowered below 200 K. We have fit the observed decay curves by assuming that the terbium emission is a forced electric dipole transition a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Austin RH,Stein DL,Wang J

更新日期：1987-03-01 00:00:00

abstract：:A structural and functional model of bacterial nitric oxide reductase (NOR) has been designed by introducing two glutamates (Glu) and three histidines (His) in sperm whale myoglobin. X-ray structural data indicate that the three His and one Glu (V68E) residues bind iron, mimicking the putative Fe(B) site in NOR, while...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin YW,Yeung N,Gao YG,Miner KD,Tian S,Robinson H,Lu Y

更新日期：2010-05-11 00:00:00

abstract：:Interdigital webbing has evolved repeatedly in tropical salamanders (bolitoglossines). This derived foot morphology is only one of many homoplastic traits in this diverse amphibian clade. Indeed, few if any morphological traits sort lineages within this clade. We investigate the processes underlying the homoplastic ev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaekel M,Wake DB

更新日期：2007-12-18 00:00:00

abstract：:Recently, we described a recurrent variant translocation, t(11;17)(q23;q21), in acute promyelocytic leukemia (APL) which juxtaposes PLZF, a gene encoding a zinc finger protein, to RARA, encoding retinoic acid receptor alpha (RAR alpha). We have now cloned cDNAs encoding PLZF-RAR alpha chimeric proteins and studied the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Z,Guidez F,Rousselot P,Agadir A,Chen SJ,Wang ZY,Degos L,Zelent A,Waxman S,Chomienne C

更新日期：1994-02-01 00:00:00

abstract：:The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villeneuve S,Souchon H,Riottot MM,Mazie JC,Lei P,Glaudemans CP,Kovác P,Fournier JM,Alzari PM

更新日期：2000-07-18 00:00:00

abstract：:During the first 2 postnatal years, cortical thickness of the human brain develops dynamically and spatially heterogeneously and likely peaks between 1 and 2 y of age. The striking development renders this period critical for later cognitive outcomes and vulnerable to early neurodevelopmental disorders. However, due t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang F,Lian C,Wu Z,Zhang H,Li T,Meng Y,Wang L,Lin W,Shen D,Li G

更新日期：2019-08-06 00:00:00

abstract：:Human PC4 and the yeast ortholog Sub1 have multiple functions in RNA polymerase II transcription. Genome-wide mapping revealed that Sub1 is present on Pol III-transcribed genes. Sub1 was found to interact with components of the Pol III transcription system and to stimulate the initiation and reinitiation steps in a sy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tavenet A,Suleau A,Dubreuil G,Ferrari R,Ducrot C,Michaut M,Aude JC,Dieci G,Lefebvre O,Conesa C,Acker J

更新日期：2009-08-25 00:00:00

abstract：:Combinations of molecular tags visible in light and electron microscopes become particularly advantageous in the analysis of dynamic cellular components like the Golgi apparatus. This organelle disassembles at the onset of mitosis and, after a sequence of poorly understood events, reassembles after cytokinesis. The pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gaietta GM,Giepmans BN,Deerinck TJ,Smith WB,Ngan L,Llopis J,Adams SR,Tsien RY,Ellisman MH

更新日期：2006-11-21 00:00:00

abstract：:Ras proteins are small GTP binding proteins that serve as critical relays in a variety of signal transduction pathways in eukaryotic cells. Like most metazoan Ras proteins, yeast Ras is post-translationally modified by addition of a farnesyl and a palmitoyl moiety, and these modifications are required for targeting th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhattacharya S,Chen L,Broach JR,Powers S

更新日期：1995-03-28 00:00:00