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Sequence dependence of hydrogen exchange kinetics in DNA duplexes at the individual base pair level in solution.

Abstract：

:The kinetics for hydrogen exchange at individual base pairs in self-complementary deoxydodecanucleotide duplexes have been estimated from NMR saturation recovery measurements on the resolved imino protons as a function of temperature. The imino protons of dA . dT base pairs in the center of the fully alternating d(C-G-C-G-T-A-T-A-C-G-C-G) duplex exchange a factor of 2- to 3-fold faster than the corresponding protons at the same positions in the partially alternating d(C-G-C-G-A-A-T-T-C-G-C-G) duplex. These exchange parameters are a direct measure of the rate constants for transient opening of individual dA . dT base pairs in the dodecanucleotide duplexes and demonstrate faster opening kinetics for the "TATA" box region compared to the related "AATT" segment.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Patel DJ,Ikuta S,Kozlowski S,Itakura K

doi

10.1073/pnas.80.8.2184

subject

Has Abstract

pub_date

1983-04-01 00:00:00

pages

2184-8

issue

8

eissn

0027-8424

issn

1091-6490

journal_volume

80

pub_type

杂志文章

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