The mammalian target of rapamycin regulates cholesterol biosynthetic gene expression and exhibits a rapamycin-resistant transcriptional profile.

abstract：:The free energy difference between complexes of the restriction nuclease EcoRI with nonspecific DNA and with the enzyme's recognition sequence is linearly dependent on the water chemical potential of the solution, set using several very different solutes, ranging from glycine and glycerol to triethylene glycol and suc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sidorova NY,Rau DC

更新日期：1996-10-29 00:00:00

abstract：:The human beta-globin locus is activated transcriptionally by a complex series of events that culminate in appropriate temporal and tissue-specific control over five separate genes during embryonic and early postnatal development. One cis-regulatory element in the locus, originally identified as an enhancer 3' to the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Q,Tanimoto K,Bungert J,Engel JD

更新日期：1998-08-18 00:00:00

abstract：:Tumor metastasis is the cause of most cancer deaths. Although metastases can form in multiple end organs, the liver is recognized as a highly permissive organ. Nevertheless, there is evidence for immune cell-mediated mechanisms that function to suppress liver metastasis by certain tumors, although the underlying mecha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kimura Y,Inoue A,Hangai S,Saijo S,Negishi H,Nishio J,Yamasaki S,Iwakura Y,Yanai H,Taniguchi T

更新日期：2016-12-06 00:00:00

abstract：:The cellular replicative DNA polymerases cannot initiate DNA synthesis without a priming 3' OH. During DNA replication, this is supplied in the context of a short RNA primer molecule synthesized by DNA primase. The primase of archaea and eukaryotes, despite having varying subunit compositions, share sequence and struc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan J,Holzer S,Pellegrini L,Bell SD

更新日期：2018-06-26 00:00:00

abstract：:The human immunodeficiency virus 1 (HIV-1) long terminal repeat (LTR) sequences were inserted into adenovirus in place of the E1 region. The HIV-1 LTR contained in this recombinant adenovirus responds to trans-activation by tatIII in a HeLa cell line constitutively expressing that HIV-1 gene product. In addition, the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rice AP,Mathews MB

更新日期：1988-06-01 00:00:00

abstract：:The pancreatic beta cell can respond in the long term to hyperglycemia both with an increased capacity for insulin production and, in susceptible individuals, with apoptosis. When glucose-induced apoptosis offsets the increasing beta cell capacity, type 2 diabetes results. Here, we tested the idea that the pathway of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu K,Paterson AJ,Chin E,Kudlow JE

更新日期：2000-03-14 00:00:00

abstract：:Life in environments devoid of photosynthesis, such as on early Earth or in contemporary dark subsurface ecosystems, is supported by chemical energy. How, when, and where chemical nutrients released from the geosphere fuel chemosynthetic biospheres is fundamental to understanding the distribution and diversity of life...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dunham EC,Dore JE,Skidmore ML,Roden EE,Boyd ES

更新日期：2021-01-12 00:00:00

abstract：:Understanding the detailed mechanism of the activation of voltage-gated ion channels has been a problem of great current interest. Reliable molecular simulations of voltage effects present a major challenge because meaningful converging microscopic simulations are not yet available and macroscopic treatments involve m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dryga A,Chakrabarty S,Vicatos S,Warshel A

更新日期：2012-02-28 00:00:00

abstract：:FIP-3 (14.7K interacting protein) was discovered during a search for cell proteins that could interact with an adenovirus protein (Ad E3-14.7K) that had been shown to prevent tumor necrosis factor (TNF)-alpha-induced cytolysis. FIP-3, which contains leucine zippers and a zinc finger domain, inhibits both basal and ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Kang J,Friedman J,Tarassishin L,Ye J,Kovalenko A,Wallach D,Horwitz MS

更新日期：1999-02-02 00:00:00

abstract：:Chondrules may have played a critical role in the earliest stages of planet formation by mediating the accumulation of dust into planetesimals. However, the origin of chondrules and their significance for planetesimal accretion remain enigmatic. Here, we show that chondrules and matrix in the carbonaceous chondrite Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Budde G,Kleine T,Kruijer TS,Burkhardt C,Metzler K

更新日期：2016-03-15 00:00:00

abstract：:The peptide composition of purified Histocompatibility-2 (H-2) reactive glycoprotein fragments from two murine strains allelic at the H-2 locus (H-2(b) and H-2(d)) were compared by a cellulose thin-layer peptide mapping micro technique. Approximately 70 per cent of the theoretical maximum number of peptides produced b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shimada A,Yamane K,Nathenson SG

更新日期：1970-03-01 00:00:00

abstract：:This study presents, to our knowledge, the first on-line measurement of acetylcholinesterase (AcChoEase) release from brain tissue. It is now well established that a soluble form of the enzyme is released from central nervous system neurons, and it has been proposed on indirect grounds that such release may occur not ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Llinás RR,Greenfield SA

更新日期：1987-05-01 00:00:00

abstract：:Fusions of the lac operon to genes induced by treatment with sublethal levels of alkylating agents have been selected from random insertions of the Mu-dl(ApRlac) phage by screening for induction of beta-galactosidase activity in the presence of methyl methanesulfonate. Genetic analysis reveals that these fusions resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Volkert MR,Nguyen DC

更新日期：1984-07-01 00:00:00

abstract：:Most transmissible spongiform encephalopathies arise either spontaneously or by infection. Mutations of PRNP, which encodes the prion protein, PrP, segregate with phenotypically similar diseases. Here we report that moderate overexpression in transgenic mice of mPrP(170N,174T), a mouse PrP with two point mutations tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sigurdson CJ,Nilsson KP,Hornemann S,Heikenwalder M,Manco G,Schwarz P,Ott D,Rülicke T,Liberski PP,Julius C,Falsig J,Stitz L,Wüthrich K,Aguzzi A

更新日期：2009-01-06 00:00:00

abstract：:A plasmid construct expressing the p66 version of the human immunodeficiency virus reverse transcriptase as a bacterial fusion protein was subjected to in vitro mutagenesis, and the resulting variant proteins were assayed to define the locations of the two major enzymatic activities. The DNA polymerase activity was lo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prasad VR,Goff SP

更新日期：1989-05-01 00:00:00

abstract：:Prolonged incubation of NIH 3T3 cells under the growth constraint of confluence results in a persistent impairment of proliferation when the cells are subcultured at low density and a greatly increased probability of neoplastic transformation in assays for transformation. These properties, along with the large accumul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chow M,Rubin H

更新日期：1996-09-03 00:00:00

abstract：:The allocation of carbon and nitrogen resources to the synthesis of plant proteins, carbohydrates, and lipids is complex and under the control of many genes; much remains to be understood about this process. QQS (Qua-Quine Starch; At3g30720), an orphan gene unique to Arabidopsis thaliana, regulates metabolic processes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Zheng W,Zhu Y,Ye H,Tang B,Arendsee ZW,Jones D,Li R,Ortiz D,Zhao X,Du C,Nettleton D,Scott MP,Salas-Fernandez MG,Yin Y,Wurtele ES

更新日期：2015-11-24 00:00:00

abstract：:We introduce a coarse-grained model for atomic glass formers. Its elements are physically motivated local microscopic dynamical rules parameterized by observables. Results of the model are established and used to interpret the measured behaviors of supercooled fluids approaching glass transitions. The model predicts t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garrahan JP,Chandler D

更新日期：2003-08-19 00:00:00

abstract：:A vaccine capable of stimulating protective antiviral antibody responses is needed to curtail the global AIDS epidemic caused by HIV-1. Although rarely elicited during the course of natural infection or upon conventional vaccination, the membrane-proximal ectodomain region (MPER) of the HIV-1 glycoprotein of M(r) 41,0...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song L,Sun ZY,Coleman KE,Zwick MB,Gach JS,Wang JH,Reinherz EL,Wagner G,Kim M

更新日期：2009-06-02 00:00:00

abstract：:Epidemiological and genetic association studies show that genetics play an important role in the attainment of education. Here, we investigate the effect of this genetic component on the reproductive history of 109,120 Icelanders and the consequent impact on the gene pool over time. We show that an educational attainm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kong A,Frigge ML,Thorleifsson G,Stefansson H,Young AI,Zink F,Jonsdottir GA,Okbay A,Sulem P,Masson G,Gudbjartsson DF,Helgason A,Bjornsdottir G,Thorsteinsdottir U,Stefansson K

更新日期：2017-01-31 00:00:00

abstract：:Preference behaviors are often established during early life, but the underlying neural circuit mechanisms remain unknown. Adapting a unique nesting behavior assay, we confirmed a "critical period" for developing music preference in C57BL/6 mice. Early music exposure between postnatal days 15 and 24 reversed their inn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang EJ,Lin EW,Hensch TK

更新日期：2012-10-16 00:00:00

abstract：:Natural ecosystems contain many individuals and species interacting with each other and with their abiotic environment. Such systems can be expected to exhibit complex dynamics in which small perturbations can be amplified to cause large changes. Here, we document the reorganization of an arid ecosystem that has occur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JH,Valone TJ,Curtin CG

更新日期：1997-09-02 00:00:00

abstract：:Fisheries-induced evolution and its impact on the productivity of exploited fish stocks remains a highly contested research topic in applied fish evolution and fisheries science. Although many quantitative models assume that larger, more fecund fish are preferentially removed by fishing, there is no empirical evidence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutter DA,Suski CD,Philipp DP,Klefoth T,Wahl DH,Kersten P,Cooke SJ,Arlinghaus R

更新日期：2012-12-18 00:00:00

abstract：:Magnetic ground states in solids often arise as a result of a delicate balance between competing factors. One currently active area of research in magnetic materials involves compounds in which long-range magnetic ordering at low temperatures is frustrated by the geometry of the crystalline lattice, a situation known ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karunadasa H,Huang Q,Ueland BG,Schiffer P,Cava RJ

更新日期：2003-07-08 00:00:00

abstract：:The influx of Ca2+ ions controls many important processes in excitable cells, including the regulation of the gating of Ca(2+)-activated K+ channels (the current IK[Ca]). Various IK[Ca] channels contribute to the regulation of the action-potential waveform, the repetitive discharge of spikes, and the secretion of neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wisgirda ME,Dryer SE

更新日期：1994-03-29 00:00:00

abstract：:Operant conditioning techniques were used to assess how gender and song familiarity affect song discrimination in adult zebra finches (Taeniopygia guttata). Twenty-five males and females, divided into five cohorts, were trained to discriminate between conspecific songs at different times of year. Males discriminating ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cynx J,Nottebohm F

更新日期：1992-02-15 00:00:00

abstract：:We have isolated cold-sensitive mutations in two genes of the yeast Saccharomyces cerevisiae, BIN2 and BIN3, that cause aberrant chromosome segregation in vivo. BIN2 and BIN3 encode essential proteins that are similar to each other and to TCP-1. TCP-1 and TCP-1-like proteins are components of the eukaryotic cytoplasmi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Sullivan DS,Huffaker TC

更新日期：1994-09-13 00:00:00

abstract：:Our understanding of the mechanisms of neural circuit assembly is far from complete. Identification of wiring molecules with novel mechanisms of action will provide insights into how complex and heterogeneous neural circuits assemble during development. In the Drosophila olfactory system, 50 classes of olfactory recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie Q,Wu B,Li J,Xu C,Li H,Luginbuhl DJ,Wang X,Ward A,Luo L

更新日期：2019-08-06 00:00:00

abstract：:The highly polymorphic system of serologically defined genetic markers on human IgG heavy chains (Gm allotypes) is second only to the HLA complex in terms of the large number of determinants, alleles, and haplotypes that can be used for analyses of disease associations and other genetic studies. However, present typin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zelaschi D,Newby C,Parsons M,van West B,Cavalli-Sforza LL,Herzenberg LA,Herzenberg LA

更新日期：1983-06-01 00:00:00

abstract：:The role of the endoplasmic reticulum stress-regulated kinase, PERK, in mammary gland function was assessed through generation of a targeted deletion in mammary epithelium. Characterization revealed that PERK is required for functional maturation of milk-secreting mammary epithelial cells. PERK-dependent signaling con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bobrovnikova-Marjon E,Hatzivassiliou G,Grigoriadou C,Romero M,Cavener DR,Thompson CB,Diehl JA

更新日期：2008-10-21 00:00:00