Abstract:
:Our understanding of the mechanisms of neural circuit assembly is far from complete. Identification of wiring molecules with novel mechanisms of action will provide insights into how complex and heterogeneous neural circuits assemble during development. In the Drosophila olfactory system, 50 classes of olfactory receptor neurons (ORNs) make precise synaptic connections with 50 classes of partner projection neurons (PNs). Here, we performed an RNA interference screen for cell surface molecules and identified the leucine-rich repeat-containing transmembrane protein known as Fish-lips (Fili) as a novel wiring molecule in the assembly of the Drosophila olfactory circuit. Fili contributes to the precise axon and dendrite targeting of a small subset of ORN and PN classes, respectively. Cell-type-specific expression and genetic analyses suggest that Fili sends a transsynaptic repulsive signal to neurites of nonpartner classes that prevents their targeting to inappropriate glomeruli in the antennal lobe.
journal_name
Proc Natl Acad Sci U S Aauthors
Xie Q,Wu B,Li J,Xu C,Li H,Luginbuhl DJ,Wang X,Ward A,Luo Ldoi
10.1073/pnas.1905832116subject
Has Abstractpub_date
2019-08-06 00:00:00pages
16068-16073issue
32eissn
0027-8424issn
1091-6490pii
1905832116journal_volume
116pub_type
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