PERK-dependent regulation of lipogenesis during mouse mammary gland development and adipocyte differentiation.

Abstract:

:The role of the endoplasmic reticulum stress-regulated kinase, PERK, in mammary gland function was assessed through generation of a targeted deletion in mammary epithelium. Characterization revealed that PERK is required for functional maturation of milk-secreting mammary epithelial cells. PERK-dependent signaling contributes to lipogenic differentiation in mammary epithelium, and perk deletion inhibits the sustained expression of lipogenic enzymes FAS, ACL, and SCD1. As a result, mammary tissue has reduced lipid content and the milk produced has altered lipid composition, resulting in attenuated pup growth. Consistent with PERK-dependent regulation of the lipogenic pathway, loss of PERK inhibits expression of FAS, ACL, and SCD1 in immortalized murine embryonic fibroblasts when cultured under conditions favoring adipocyte differentiation. These findings implicate PERK as a physiologically relevant regulator of the lipogenic pathway.

authors

Bobrovnikova-Marjon E,Hatzivassiliou G,Grigoriadou C,Romero M,Cavener DR,Thompson CB,Diehl JA

doi

10.1073/pnas.0808517105

subject

Has Abstract

pub_date

2008-10-21 00:00:00

pages

16314-9

issue

42

eissn

0027-8424

issn

1091-6490

pii

0808517105

journal_volume

105

pub_type

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