Induction of specific Escherichia coli genes by sublethal treatments with alkylating agents.

abstract：:The Cdc7p protein kinase is essential for the G1/S transition and initiation of DNA replication during the cell division cycle in Saccharomyces cerevisiae. Cdc7p appears to be an evolutionarily conserved protein, since a homolog Hsk1 has been isolated from Schizosaccharomyces pombe. Here, we report the isolation of a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang W,Hunter T

更新日期：1997-12-23 00:00:00

abstract：:A technique involving 5-bromodeoxyuridine, 33258 Hoechst, and fluorescence microscopy has been used to analyze replication kinetics in cells from embryonic and adult mice bearing the Cattanach [T(X;7)ICt] translocation in a balanced or an unbalanced form. In balanced 9- and 13-day female embryos, the translocated X wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Disteche CM,Eicher EM,Latt SA

更新日期：1979-10-01 00:00:00

abstract：:It is well accepted that the induction of endothelial cell (EC) adhesion molecules is a critical component in acute inflammatory responses as well as allogeneic interactions in vascularized allografts and, possibly, atherogenesis. The "inflammatory triad" of interleukin 1 (IL-1), tumor necrosis factor, and lipopolysac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bender JR,Sadeghi MM,Watson C,Pfau S,Pardi R

更新日期：1994-04-26 00:00:00

abstract：:The androgen receptor (AR) binds to androgen response elements and regulates target genes via a mechanism involving coregulators. Here we demonstrate that the AR can interact with the testicular orphan receptor-4 (TR4) and function as a repressor to down-regulate the TR4 target genes by preventing the TR4 binding to i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee YF,Shyr CR,Thin TH,Lin WJ,Chang C

更新日期：1999-12-21 00:00:00

abstract：:The Escherichia coli strain, ts-rnp5, originally described in 1975 by G. D. Burdick and H. Berger, is shown to possess an RNA polymerase (RNA nucleotidyltransferase) sigma subunit with an activity 4--6 times less thermostable at 45 degrees than sigma from wild-type strains. This defect remains associated with the sigm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross C,Hoffman J,Ward C,Hager D,Burdick G,Berger H,Burgess R

更新日期：1978-01-01 00:00:00

abstract：:This article briefly summarizes the research activities in the field of hydrogen storage in sorbent materials and reports our recent works and future directions for the design of such materials. Distinct features of sorption-based hydrogen storage methods are described compared with metal hydrides and complex chemical...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park N,Choi K,Hwang J,Kim DW,Kim DO,Ihm J

更新日期：2012-12-04 00:00:00

abstract：:Most paleo-episodes of ocean acidification (OA) were either too slow or too small to be instructive in predicting near-future impacts. The end-Cretaceous event (66 Mya) is intriguing in this regard, both because of its rapid onset and also because many pelagic calcifying species (including 100% of ammonites and more t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyrrell T,Merico A,Armstrong McKay DI

更新日期：2015-05-26 00:00:00

abstract：:A chimeric mouse-human antibody has been created that recognizes an antigen found on the surface of cells from many carcinomas. Immunoglobulin constant (C) domains of the mouse monoclonal antibody L6, C gamma 2a and C kappa, were substituted by the human C gamma 1 and C kappa by recombining cDNA modules encoding varia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu AY,Robinson RR,Hellström KE,Murray ED Jr,Chang CP,Hellström I

更新日期：1987-05-01 00:00:00

abstract：:We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kasianowicz JJ,Brandin E,Branton D,Deamer DW

更新日期：1996-11-26 00:00:00

abstract：:Establishing a timeline for the evolution of novelties is a common, unifying goal at the intersection of evolutionary and developmental biology. Analyses of gene regulatory networks (GRNs) provide the ability to understand the underlying genetic and developmental mechanisms responsible for the origin of morphological ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson JR,Erkenbrack EM,Hinman VF,McCauley BS,Petsios E,Bottjer DJ

更新日期：2017-06-06 00:00:00

abstract：:Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harvie NR,Schultz JS

更新日期：1970-05-01 00:00:00

abstract：:We discuss the generic time behavior of reaction-diffusion processes capable of modeling various types of biological transport processes, such as ligand migration in proteins and gating fluctuations in ion channel proteins. The main observable in these two cases, the fraction of unbound ligands and the probability of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadler W,Stein DL

更新日期：1991-08-01 00:00:00

abstract：:Activated RAS transforming genes that encode proteins (p21s) with amino acid substitutions at positions 12, 13, or 61 have been detected in 10-20% of human neoplasms. This report describes a monoclonal antibody (DWP) raised against a synthetic peptide corresponding to amino acids 5-16 of a mutated RAS gene encoding Va...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carney WP,Petit D,Hamer P,Der CJ,Finkel T,Cooper GM,Lefebvre M,Mobtaker H,Delellis R,Tischler AS

更新日期：1986-10-01 00:00:00

abstract：:Although melanomas with mutant v-Raf murine sarcoma viral oncogene homolog B1 (BRAF) can now be effectively targeted, there is no molecular target for most melanomas expressing wild-type BRAF. Here, we show that the activation of Pleckstrin homology domain-interacting protein (PHIP), promotes melanoma metastasis, can ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Semir D,Nosrati M,Bezrookove V,Dar AA,Federman S,Bienvenu G,Venna S,Rangel J,Climent J,Meyer Tamgüney TM,Thummala S,Tong S,Leong SP,Haqq C,Billings P,Miller JR 3rd,Sagebiel RW,Debs R,Kashani-Sabet M

更新日期：2012-05-01 00:00:00

abstract：:The proteins cdc2 and cyclin form a heterodimer (maturation promoting factor) that controls the major events of the cell cycle. A mathematical model for the interactions of cdc2 and cyclin is constructed. Simulation and analysis of the model show that the control system can operate in three modes: as a steady state wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tyson JJ

更新日期：1991-08-15 00:00:00

abstract：:We have used expression cloning in Xenopus oocytes to isolate two different cDNAs encoding functional N-methyl-D-aspartate (NMDA) receptor subunits. The two receptors (NMDA-R1A and -R1B) display different pharmacologic properties as a consequence of alternative exon addition within the putative ligand-binding domain. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakanishi N,Axel R,Shneider NA

更新日期：1992-09-15 00:00:00

abstract：:Although it is accepted that regulatory T cells (T regs) contribute to cancer progression, most studies in the field consider nonantigen-specific suppression. Here, we show the presence of tumor antigen-specific CD4(+) T regs in the blood of patients with metastatic melanoma. These CD4(+) T regs recognize a broad rang...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vence L,Palucka AK,Fay JW,Ito T,Liu YJ,Banchereau J,Ueno H

更新日期：2007-12-26 00:00:00

abstract：:The neural cell adhesion molecule N-CAM is a sialic acid-rich, cell surface glycoprotein that mediates cell adhesion by a homophilic mechanism. Its binding function has been implicated in both morphogenesis and histogenesis; during development it changes in amount at the cell surface and perinatally it undergoes a dec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman S,Chuong CM,Edelman GM

更新日期：1984-11-01 00:00:00

abstract：:In this contribution, we study situations in which nanoparticles in a fluid are strongly heated, generating high heat fluxes. This situation is relevant to experiments in which a fluid is locally heated by using selective absorption of radiation by solid particles. We first study this situation for different types of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Merabia S,Shenogin S,Joly L,Keblinski P,Barrat JL

更新日期：2009-09-08 00:00:00

abstract：:Recently, we described a recurrent variant translocation, t(11;17)(q23;q21), in acute promyelocytic leukemia (APL) which juxtaposes PLZF, a gene encoding a zinc finger protein, to RARA, encoding retinoic acid receptor alpha (RAR alpha). We have now cloned cDNAs encoding PLZF-RAR alpha chimeric proteins and studied the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Z,Guidez F,Rousselot P,Agadir A,Chen SJ,Wang ZY,Degos L,Zelent A,Waxman S,Chomienne C

更新日期：1994-02-01 00:00:00

abstract：:Aphids maintain mutualistic symbioses involving consortia of coinherited organisms. All possess a primary endosymbiont, Buchnera, which compensates for dietary deficiencies; many also contain secondary symbionts, such as Hamiltonella defensa, which confers defense against natural enemies. Genome sequences of uncultiva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moran NA,Degnan PH,Santos SR,Dunbar HE,Ochman H

更新日期：2005-11-22 00:00:00

abstract：:Soil organisms, as recorded by trace fossils in paleosols of the Willwood Formation, Wyoming, show significant body-size reductions and increased abundances during the Paleocene-Eocene Thermal Maximum (PETM). Paleobotanical, paleopedologic, and oxygen isotope studies indicate high temperatures during the PETM and shar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith JJ,Hasiotis ST,Kraus MJ,Woody DT

更新日期：2009-10-20 00:00:00

abstract：:To achieve the mission of personalized medicine, centering on delivering the right drug to the right patient at the right dose, therapeutic drug monitoring solutions are necessary. In that regard, wearable biosensing technologies, capable of tracking drug pharmacokinetics in noninvasively retrievable biofluids (e.g., ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin S,Yu W,Wang B,Zhao Y,En K,Zhu J,Cheng X,Zhou C,Lin H,Wang Z,Hojaiji H,Yeung C,Milla C,Davis RW,Emaminejad S

更新日期：2020-08-11 00:00:00

abstract：:The assembly of actin monomers into filaments and networks plays vital roles throughout eukaryotic biology, including intracellular transport, cell motility, cell division, determining cellular shape, and providing cells with mechanical strength. The regulation of actin assembly and modulation of filament mechanical p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang H,Bradley MJ,McCullough BR,Pierre A,Grintsevich EE,Reisler E,De La Cruz EM

更新日期：2012-10-16 00:00:00

abstract：:Large-scale conformational changes in proteins are often associated with the binding of a substrate. Because conformational changes may be related to the function of an enzyme, understanding the kinetics and energetics of these motions is very important. We have delineated the atomically detailed conformational transi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arora K,Brooks CL 3rd

更新日期：2007-11-20 00:00:00

abstract：:Deciphering the electron transfer reactivity characteristics of amyloid β-peptide copper complexes is an important task in connection with the role they are assumed to play in Alzheimer's disease. A systematic analysis of this question with the example of the amyloid β-peptide copper complex by means of its electroche...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balland V,Hureau C,Savéant JM

更新日期：2010-10-05 00:00:00

abstract：:The DNA origami method permits folding of long single-stranded DNA into complex 3D structures with subnanometer precision. Transmission electron microscopy, atomic force microscopy, and recently cryo-EM tomography have been used to characterize the properties of such DNA origami objects, however their microscopic stru...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoo J,Aksimentiev A

更新日期：2013-12-10 00:00:00

abstract：:Excitatory amino acid transporters (EAATs) buffer and remove synaptically released L-glutamate and maintain its concentrations below neurotoxic levels. EAATs also mediate a thermodynamically uncoupled substrate-gated anion conductance that may modulate cell excitability. Here, we demonstrate that modification of a cys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seal RP,Shigeri Y,Eliasof S,Leighton BH,Amara SG

更新日期：2001-12-18 00:00:00

abstract：:Biomimetic nanopores based on membrane-spanning single-walled carbon nanotubes have been designed to include selectivity filters based on combinations of anionic and cationic groups mimicking those present in bacterial porins and in voltage-gated sodium and calcium channels. The ion permeation and selectivity properti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： García-Fandiño R,Sansom MS

更新日期：2012-05-01 00:00:00

abstract：:The spectrum of cellular targets in the autoimmune diseases is both large and varied and includes among the nuclear components the so-called Sm and RNP antigens associated with systemic lupus erythematosus. The use of immunoaffinity chromatography with dual specificity for the Sm and RNP antigens has allowed for their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White PJ,Gardner WD,Hoch SO

更新日期：1981-01-01 00:00:00