Mutation affecting thermostability of sigma subunit of Escherichia coli RNA polymerase lies near the dnaG locus at about 66 min on the E. coli genetic map.

abstract：:A strain of Escherichia coli, selected on the basis of its resistance to colicin N, reveals distinct structural and functional alterations in unspecific OmpF porin. A single mutation [Gly-119-->Asp (G119D)] was identified in the internal loop L3 that contributes critically to the formation of the construction inside t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jeanteur D,Schirmer T,Fourel D,Simonet V,Rummel G,Widmer C,Rosenbusch JP,Pattus F,Pagès JM

更新日期：1994-10-25 00:00:00

abstract：:Structure and function of p185neu receptor tyrosine kinase were found to be regulated in a cell cycle-dependent manner. In M phase, p185neu is hyperphosphorylated at serine and/or threonine residues. The phosphotyrosine [Tyr(P)] content of p185neu is at its highest level in G0/G1 phase, decreases through S and G2 phas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kiyokawa N,Yan DH,Brown ME,Hung MC

更新日期：1995-02-14 00:00:00

abstract：:Deletion derivatives of the maize transposable element Activator (Ac) were constructed in vitro and inserted into a kanamycin resistance gene. These constructions were then introduced into tobacco protoplasts derived from plants previously transformed with Ac. The ability of each deletion derivative to excise was meas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coupland G,Plum C,Chatterjee S,Post A,Starlinger P

更新日期：1989-12-01 00:00:00

abstract：:How likely is it that someone would approve of using a nuclear weapon to kill millions of enemy civilians in the hope of ending a ground war that threatens thousands of American troops? Ask them how they feel about prosecuting immigrants, banning abortion, supporting the death penalty, and protecting gun rights and yo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Slovic P,Mertz CK,Markowitz DM,Quist A,Västfjäll D

更新日期：2020-08-25 00:00:00

abstract：:There is no theoretical limit in using molecular networks to harvest diffusive sun photons on large areas and funnel them onto much smaller areas of highly efficient but also precious energy-converting materials. The most effective concept reported so far is based on a pool of randomly oriented, light-harvesting donor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willich MM,Wegener L,Vornweg J,Hohgardt M,Nowak J,Wolter M,Jacob CR,Walla PJ

更新日期：2020-12-29 00:00:00

abstract：:Deficiency of acyl CoA:cholesterol acyltransferase 2 (ACAT2) in mice results in a reduction in cholesterol ester synthesis in the small intestine and liver, which in turn limits intestinal cholesterol absorption, hepatic cholesterol gallstone formation, and the accumulation of cholesterol esters in the plasma lipoprot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willner EL,Tow B,Buhman KK,Wilson M,Sanan DA,Rudel LL,Farese RV Jr

更新日期：2003-02-04 00:00:00

abstract：:p70 S6 kinase (p70(S6k)) is a mitogen-activated protein kinase that plays a central role in the control of mRNA translation. It physiologically phosphorylates the S6 protein of the 40s ribosomal subunit in response to mitogenic stimuli and is a downstream component of the rapamycin-sensitive pathway, which includes th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burnett PE,Blackshaw S,Lai MM,Qureshi IA,Burnett AF,Sabatini DM,Snyder SH

更新日期：1998-07-07 00:00:00

abstract：:Effects of the anti-T-cell monoclonal antibodies OKT3, OKT5, and OKT8 on T-cell surface properties and cell functions were evaluated. Incubation of mononuclear cells isolated from peripheral blood for 48 hr with each monoclonal antibody in the absence of complement resulted in modulation of their respective surface an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Antel J,Oger JJ,Jackevicius S,Kuo HH,Arnason BG

更新日期：1982-05-01 00:00:00

abstract：:Disruption of the nucleotide excision repair (NER) pathway by mutations can cause xeroderma pigmentosum, a syndrome predisposing affected individuals to development of skin cancer. The xeroderma pigmentosum C (XPC) protein is essential for initiating global genome NER by recognizing the DNA lesion and recruiting downs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ming M,Shea CR,Guo X,Li X,Soltani K,Han W,He YY

更新日期：2010-12-28 00:00:00

abstract：:The soil-transmitted helminths or nematodes (hookworms, whipworms, and Ascaris) are roundworms that infect more than 1 billion of the poorest peoples and are leading causes of morbidity worldwide. Few anthelmintics are available for treatment, and only one is commonly used in mass drug administrations. New anthelminti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Y,Platzer EG,Bellier A,Aroian RV

更新日期：2010-03-30 00:00:00

abstract：:Excitatory amino acid transporters (EAATs) buffer and remove synaptically released L-glutamate and maintain its concentrations below neurotoxic levels. EAATs also mediate a thermodynamically uncoupled substrate-gated anion conductance that may modulate cell excitability. Here, we demonstrate that modification of a cys...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seal RP,Shigeri Y,Eliasof S,Leighton BH,Amara SG

更新日期：2001-12-18 00:00:00

abstract：:The recent completion of the deletion of essentially all of the ORFs in yeast is an important new resource for identifying the phenotypes of unknown genes. Each ORF is replaced with a cassette containing unique tag sequences that allow rapid parallel analysis of strains in a pool by using hybridization to a high-densi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birrell GW,Giaever G,Chu AM,Davis RW,Brown JM

更新日期：2001-10-23 00:00:00

abstract：:4"'-Diazomalonyldigitoxin and its isomer, 3"'-diazomalonyldigitoxin, have been synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall C,Ruoho A

更新日期：1980-08-01 00:00:00

abstract：:Saposin A, a heat-stable 16-kDa glycoprotein, was isolated from Gaucher disease spleen and purified to homogeneity. Chemical sequencing from its amino terminus and of peptides obtained by digestion with protease from Staphylococcus aureus strain V-8 demonstrated that saposin A is derived from proteolytic processing of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morimoto S,Martin BM,Yamamoto Y,Kretz KA,O'Brien JS,Kishimoto Y

更新日期：1989-05-01 00:00:00

abstract：:Ancient DNA (aDNA) analysis provides a powerful means of investigating human migration, social organization, and a plethora of other crucial questions about humanity's past. Recently, specialists have suggested that the ideal research design involving aDNA would include multiple independent lines of evidence. In this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bongers JL,Nakatsuka N,O'Shea C,Harper TK,Tantaleán H,Stanish C,Fehren-Schmitz L

更新日期：2020-08-04 00:00:00

abstract：:Protein tyrosine phosphatase (PTP)σ (PTPRS) was shown previously to be associated with susceptibility to inflammatory bowel disease (IBD). PTPσ(-/-) mice exhibit an IBD-like phenotype in the intestine and show increased susceptibility to acute models of murine colitis. However, the function of PTPσ in the intestine is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murchie R,Guo CH,Persaud A,Muise A,Rotin D

更新日期：2014-01-14 00:00:00

abstract：:A central unresolved issue in evolutionary biology is how metabolic innovations emerge. Low-level enzymatic side activities are frequent and can potentially be recruited for new biochemical functions. However, the role of such underground reactions in adaptation toward novel environments has remained largely unknown a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Notebaart RA,Szappanos B,Kintses B,Pál F,Györkei Á,Bogos B,Lázár V,Spohn R,Csörgő B,Wagner A,Ruppin E,Pál C,Papp B

更新日期：2014-08-12 00:00:00

abstract：:A model chemical network involving reactions and diffusion is studied. Spatially and temporally ordered solutions of the equations are found by bifurcation theory. These solutions are calculated analytically and their stability is studied. Properties of these dissipative structures are discussed, and a comparison with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicolis G,Auchmuty JF

更新日期：1974-07-01 00:00:00

abstract：:The quaternary structure of delta-aminolevulinic acid dehydratase (5-aminolaevulinate hydrolyase, EC 4.2.1.24) from bovine liver was examined by analytical ultracentrifugation, polyacrylamide gel electrophoresis, and electron microscopy. The molecular weights, determined by sedimentation-velocity and sedimentation-equ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu WH,Shemin D,Richards KE,Williams RC

更新日期：1974-05-01 00:00:00

abstract：:In Escherichia coli, acetyl phosphate can be formed from acetyl-CoA via the phosphotransacetylase (phosphate acetyltransferase; acetyl-CoA:orthophosphate acetyltransferase, EC 2.3.1.8) reaction and from acetate (plus ATP) via the acetate kinase (ATP:acetate phosphotransferase, EC 2.7.2.1) reaction. By restricting acet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong JS,Hunt AG,Masters PS,Lieberman MA

更新日期：1979-03-01 00:00:00

abstract：:Hepatocellular injury during hepatitis B virus (HBV) infection has been postulated to result from a human leukocyte antigen (HLA)-restricted T-lymphocyte host immune response against HBV antigens. Although HLA expression is enhanced in the presence of hepatic inflammation, whether HBV itself can induce HLA expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu KQ,Vierling JM,Siddiqui A

更新日期：1990-09-01 00:00:00

abstract：:Genetic switches based on the [Formula: see text] system are master regulators of an array of cellular responses. Recent kinetic experiments have shown that [Formula: see text] can actively remove NF-κB bound to its genetic sites via a process called "molecular stripping." This allows the [Formula: see text] switch to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Potoyan DA,Zheng W,Komives EA,Wolynes PG

更新日期：2016-01-05 00:00:00

abstract：:The domestication of cultivated barley has been used as a model system for studying the origins and early spread of agrarian culture. Our previous results indicated that the Tibetan Plateau and its vicinity is one of the centers of domestication of cultivated barley. Here we reveal multiple origins of domesticated bar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai F,Chen ZH,Wang X,Li Z,Jin G,Wu D,Cai S,Wang N,Wu F,Nevo E,Zhang G

更新日期：2014-09-16 00:00:00

abstract：:Preincubation of quiescent BALB/C3T3 cells with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) in conditioned medium resulted in a time- and concentration-dependent stimulation of uptake of 2-deoxyglucose, uridine, and alpha-aminoisobutyric acid over that seen in conditioned medium alone. The apparent Km values ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Natraj CV,Datta P

更新日期：1978-08-01 00:00:00

abstract：:For centuries, traditional agricultural systems have contributed to food and livelihood security throughout the world. Recognizing the ecological legacy in the traditional agricultural systems may help us develop novel sustainable agriculture. We examine how rice-fish coculture (RF), which has been designated a "globa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xie J,Hu L,Tang J,Wu X,Li N,Yuan Y,Yang H,Zhang J,Luo S,Chen X

更新日期：2011-12-13 00:00:00

abstract：:Two human 12-lipoxygenase enzyme (arachidonate:oxygen 12-oxidoreductase, EC 1.13.11.31)-related genes were characterized from 13 distinct clones isolated from three genomic bacteriophage and cosmid libraries. A complete gene (12-lipoxygenase gene 1) spanning approximately 17 kilobases and consisting of 14 exons with s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Funk CD,Funk LB,FitzGerald GA,Samuelsson B

更新日期：1992-05-01 00:00:00

abstract：:The multiplication in gases of ionization electrons, by the effect of the electric fields between parallel electrodes, leads to the emission of light from the molecules excited in the avalanche process. The optical imaging of this light, with intensifiers, on charge-coupled devices permits the localization, in the gas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charpak G,Dominik W,Zaganidis N

更新日期：1989-03-01 00:00:00

abstract：:Active matter, both synthetic and biological, demonstrates complex spatiotemporal self-organization and the emergence of collective behavior. A coherent rotational motion, the vortex phase, is of great interest because of its ability to orchestrate well-organized motion of self-propelled particles over large distances...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han K,Kokot G,Tovkach O,Glatz A,Aranson IS,Snezhko A

更新日期：2020-05-05 00:00:00

abstract：:Infection during the neonatal period commonly induces apnea episodes, and the proinflammatory cytokine IL-1beta may serve as a critical mediator between these events. To determine the mechanism by which IL-1beta depresses respiration, we examined a prostaglandin E(2) (PGE(2))-dependent pathway in newborn mice and huma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hofstetter AO,Saha S,Siljehav V,Jakobsson PJ,Herlenius E

更新日期：2007-06-05 00:00:00

abstract：:The mammalian target of rapamycin (mTOR) is a central regulator of cell growth and proliferation in response to growth factor and nutrient signaling. Consequently, this kinase is implicated in metabolic diseases including cancer and diabetes, so there is great interest in understanding the complete spectrum of mTOR-re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang BT,Ducker GS,Barczak AJ,Barbeau R,Erle DJ,Shokat KM

更新日期：2011-09-13 00:00:00