Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli.

abstract：:We report the discovery of mammalian tribosphenic teeth from the basal Cenomanian of southwestern France that we refer to a new primitive marsupial-like form identified as a basal taxon of Marsupialiformes, a new clade recognized here to include the crown group Marsupialia and primitive stem lineages more closely rela...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vullo R,Gheerbrant E,de Muizon C,Néraudeau D

更新日期：2009-11-24 00:00:00

abstract：:Previous reports of a relatively air-stable soluble hydrogenase from the photosynthetic anaerobe, Chromatium vinosum, have been substantiated. The properties of this enzyme, as seen in highly purified samples prepared by procedures that permit improved yields, are described. A possible role for flavin mediation is not...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kakuno T,Kaplan NO,Kamen MD

更新日期：1977-03-01 00:00:00

abstract：:Loss or reduction in function of tumor suppressor genes contributes to tumorigenesis. Here, by allelic DNA copy number analysis using single-nucleotide polymorphism genotyping array and mass spectrometry, we report homozygous deletion in glioblastoma multiformes at chromosome 13q21, where DACH1 gene is located. We fou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe A,Ogiwara H,Ehata S,Mukasa A,Ishikawa S,Maeda D,Ueki K,Ino Y,Todo T,Yamada Y,Fukayama M,Saito N,Miyazono K,Aburatani H

更新日期：2011-07-26 00:00:00

abstract：:Streptococcus pneumoniae is a major human pathogen that successfully adapts to the host environment via an efficient uptake system for free DNA liberated from other organisms in the upper respiratory tract, facilitating immune evasion and drug resistance. Although the initial signaling events leading to pneumococcal c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balaban M,Bättig P,Muschiol S,Tirier SM,Wartha F,Normark S,Henriques-Normark B

更新日期：2014-02-18 00:00:00

abstract：:Ovarian cancer remains the most lethal gynecologic malignancy. We analyzed the mutational landscape of 64 primary, 41 metastatic, and 17 recurrent fresh-frozen tumors from 77 patients along with matched normal DNA, by whole-exome sequencing (WES). We also sequenced 13 pairs of synchronous bilateral ovarian cancer (SBO...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li C,Bonazzoli E,Bellone S,Choi J,Dong W,Menderes G,Altwerger G,Han C,Manzano A,Bianchi A,Pettinella F,Manara P,Lopez S,Yadav G,Riccio F,Zammataro L,Zeybek B,Yang-Hartwich Y,Buza N,Hui P,Wong S,Ravaggi A,Bigno

更新日期：2019-01-08 00:00:00

abstract：:The importance of carbon in Earth's mantle greatly exceeds its modest abundance of approximately 1,000-4,000 ppm. Carbon is a constituent of key terrestrial volatiles (CO, CO(2), CH(4)), it forms diamonds, and it may also contribute to the bulk electrical properties of the silicate Earth. In contrast to that of the ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hayden LA,Watson EB

更新日期：2008-06-24 00:00:00

abstract：:Lifelong interactions between host and the ubiquitous and persistent cytomegalovirus (CMV) have been proposed to contribute to the age-related decline in immunity. Prior work from us and others found some support for that idea, yet evidence that this led to increased vulnerability to other infections was not obtained....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smithey MJ,Venturi V,Davenport MP,Buntzman AS,Vincent BG,Frelinger JA,Nikolich-Žugich J

更新日期：2018-07-17 00:00:00

abstract：:To identify mannosyl (Man)-containing intermediates of the human glycoinositol phospholipid (GPI) anchor pathway and examine their expression in paroxysmal nocturnal hemoglobinuria (PNH), mannolipid products deriving from in vitro guanosine diphosphate [3H]Man labeling of HeLa cell microsomes were characterized. The d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirose S,Ravi L,Prince GM,Rosenfeld MG,Silber R,Andresen SW,Hazra SV,Medof ME

更新日期：1992-07-01 00:00:00

abstract：:The major protein constituent of human plasma high density lipoproteins has been isolated and its complete amino-acid sequence determined. The protein, designated apolipoprotein-glutamine-I by the presence of carboxyl-terminal glutamine, is a single polypeptide chain of 245 amino-acid residues, including three residue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baker HN,Delahunty T,Gotto AM Jr,Jackson RL

更新日期：1974-09-01 00:00:00

abstract：:Primary tumors secrete factors that alter the microenvironment of distant organs, rendering those organs as fertile soil for subsequent metastatic cancer cell colonization. Although the lungs are exposed to these factors ubiquitously, lung metastases usually develop as a series of discrete lesions. The underlining mol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hiratsuka S,Goel S,Kamoun WS,Maru Y,Fukumura D,Duda DG,Jain RK

更新日期：2011-03-01 00:00:00

abstract：:Double-positive (DP) thymocytes respond to intrathymic T-cell receptor (TCR) signals by undergoing positive selection and lineage differentiation into single-positive (SP) mature cells. Concomitant with these well-characterized events is the acquisition of a mature T-cell gene expression program characterized by the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi LZ,Saravia J,Zeng H,Kalupahana NS,Guy CS,Neale G,Chi H

更新日期：2017-01-03 00:00:00

abstract：:Muscular dystrophy is a general term encompassing muscle disorders that cause weakness and wasting, typically leading to premature death. Membrane instability, as a result of a genetic disruption within the dystrophin-glycoprotein complex (DGC), is thought to induce myofiber degeneration, although the downstream mecha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millay DP,Goonasekera SA,Sargent MA,Maillet M,Aronow BJ,Molkentin JD

更新日期：2009-11-10 00:00:00

abstract：:Three enzyme reactions have been reported to catalyze the synthesis of phosphoribosylamine in eukaryotic cells. These activities are glutamine phosphoribosylpyrophosphate (P-Rib-P-P) amidotransferase [amidophosphoribosyl-transferase; 5-phosphoribosylamine: pyrophosphate phosphoribosyltransferase (glutamate-amidating) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Holmes EW,King GL,Leyva A,Singer SC

更新日期：1976-07-01 00:00:00

abstract：:Translation elongation factor P (EF-P) stimulates ribosomal peptidyltransferase activity. EF-P is conserved in bacteria and is essential for cell viability. Eukarya and Archaea have an EF-P homologue, eukaryotic initiation factor 5A (eIF-5A). In the present study, we determined the crystal structure of EF-P from Therm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hanawa-Suetsugu K,Sekine S,Sakai H,Hori-Takemoto C,Terada T,Unzai S,Tame JR,Kuramitsu S,Shirouzu M,Yokoyama S

更新日期：2004-06-29 00:00:00

abstract：:Smooth muscle cells use fibronectin to bind to type I and type III collagens but bind to type V collagen by a trypsin-resistant intrinsic glycoconjugate. The binding site on type V collagen is located in the alpha A chain. By using collagen-coated filters in a modified Boyden chamber assay for chemotaxis, it was obser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grotendorst GR,Seppä HE,Kleinman HK,Martin GR

更新日期：1981-06-01 00:00:00

abstract：:Transforming growth factor betas (TGF-betas) regulate key aspects of embryonic development and major human diseases. Although Smad2, Smad3, and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases (MAPKs) have been proposed as key mediators in TGF-beta signaling, their functional specificities...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang YC,Piek E,Zavadil J,Liang D,Xie D,Heyer J,Pavlidis P,Kucherlapati R,Roberts AB,Böttinger EP

更新日期：2003-09-02 00:00:00

abstract：:The tubercle bacillus parasitizes macrophages by inhibiting phagosome maturation into the phagolysosome. This phenomenon underlies the tuberculosis pandemic involving 2 billion people. We report here how Mycobacterium tuberculosis causes phagosome maturation arrest. A glycosylated M. tuberculosis phosphatidylinositol ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fratti RA,Chua J,Vergne I,Deretic V

更新日期：2003-04-29 00:00:00

abstract：:Genetic evidence indicates that the PrlA (SecY) protein of Escherichia coli functions as a membrane integrated signal sequence receptor in protein "export"--i.e., in protein translocation across (or integration into) the plasma membrane. We have raised antibodies in rabbits against two synthetic peptides representing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe M,Blobel G

更新日期：1989-03-01 00:00:00

abstract：:VIP21/caveolin is localized to both caveolae and apical transport vesicles and presumably cycles between the cell surface and the Golgi complex. We have studied the lipid interactions of this protein by reconstituting Escherichia coli-expressed VIP21/caveolin into liposomes. Surprisingly, the protein reconstituted onl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murata M,Peränen J,Schreiner R,Wieland F,Kurzchalia TV,Simons K

更新日期：1995-10-24 00:00:00

abstract：:In a classic model of mammalian brain formation, precursors of principal glutamatergic neurons migrate radially along radial glia fibers whereas GABAergic interneuron precursors migrate tangentially. These migration modes have significant implications for brain function. Here we used clonal lineage tracing of active r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun GJ,Zhou Y,Stadel RP,Moss J,Yong JH,Ito S,Kawasaki NK,Phan AT,Oh JH,Modak N,Reed RR,Toni N,Song H,Ming GL

更新日期：2015-07-28 00:00:00

abstract：:Bacteria commonly grow in densely populated surface-bound communities, termed biofilms, where they gain benefits including superior access to nutrients and resistance to environmental insults. The secretion of extracellular polymeric substances (EPS), which bind bacterial collectives together, is ubiquitously associat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nadell CD,Bassler BL

更新日期：2011-08-23 00:00:00

abstract：:Stomatal pores are microscopic structures on the epidermis of leaves formed by 2 specialized guard cells that control the exchange of water vapor and CO(2) between plants and the atmosphere. Stomatal size (S) and density (D) determine maximum leaf diffusive (stomatal) conductance of CO(2) (g(c(max))) to sites of assim...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Franks PJ,Beerling DJ

更新日期：2009-06-23 00:00:00

abstract：:New approaches to the study of early hominin diets have refreshed interest in how and when our diets diverged from those of other African apes. A trend toward significant consumption of C4 foods in hominins after this divergence has emerged as a landmark event in human evolution, with direct evidence provided by stabl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wynn JG,Alemseged Z,Bobe R,Grine FE,Negash EW,Sponheimer M

更新日期：2020-09-08 00:00:00

abstract：:In insulin resistant states such as type 2 diabetes, there is a high demand on the β-cell to synthesize and secrete insulin, which challenges the ability of the endoplasmic reticulum (ER) to synthesize and fold nascent proteins. This creates a state of ER stress that triggers a coordinated program referred to as the u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Winnay JN,Dirice E,Liew CW,Kulkarni RN,Kahn CR

更新日期：2014-01-21 00:00:00

abstract：:Seeking research funding is an essential part of academic life. Funded projects are primarily collaborative in nature through internal and external partnerships, but what role does funding play in the formulation of these partnerships? Here, by examining over 43,000 scientific projects funded over the past three decad...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma A,Mondragón RJ,Latora V

更新日期：2015-12-01 00:00:00

abstract：:Alzheimer's disease is characterized by two primary pathological features: amyloid plaques and neurofibrillary tangles. The interconnection between amyloid and tau aggregates is of intense interest, but mouse models have yet to reveal a direct interrelationship. We now show that NO may be a key factor that connects am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colton CA,Vitek MP,Wink DA,Xu Q,Cantillana V,Previti ML,Van Nostrand WE,Weinberg JB,Dawson H

更新日期：2006-08-22 00:00:00

abstract：:Circadian timekeeping by intracellular molecular clocks is evident widely in prokaryotes and eukaryotes. The clockworks are driven by autoregulatory feedback loops that lead to oscillating levels of components whose maxima are in fixed phase relationships with one another. These phase relationships are the key metric ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mirsky HP,Liu AC,Welsh DK,Kay SA,Doyle FJ 3rd

更新日期：2009-07-07 00:00:00

abstract：:We propose that retroviruses exploit a cell-encoded pathway of intercellular vesicle traffic, exosome exchange, for both the biogenesis of retroviral particles and a low-efficiency but mechanistically important mode of infection. This Trojan exosome hypothesis reconciles current paradigms of retrovirus-directed transm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gould SJ,Booth AM,Hildreth JE

更新日期：2003-09-16 00:00:00

abstract：:Apurinic/apyrimidinic (AP) sites are common DNA lesions that arise from spontaneous depurination or by base excision repair (BER) of modified bases. A biotin-containing aldehyde-reactive probe (ARP) [Kubo, K., Ide, H., Wallace, S. S. & Kow, Y. W. (1992) Biochemistry 31, 3703-3708] is used to measure AP sites in living...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atamna H,Cheung I,Ames BN

更新日期：2000-01-18 00:00:00

abstract：:Steep topography, a tropical climate, and mafic lithologies contribute to efficient chemical weathering and carbon sequestration in the Southeast Asian islands. Ongoing arc-continent collision between the Sunda-Banda arc system and Australia has increased the area of subaerially exposed land in the region since the mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park Y,Maffre P,Goddéris Y,Macdonald FA,Anttila ESC,Swanson-Hysell NL

更新日期：2020-10-13 00:00:00