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Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.

Abstract：

:Genetic evidence indicates that the PrlA (SecY) protein of Escherichia coli functions as a membrane integrated signal sequence receptor in protein "export"--i.e., in protein translocation across (or integration into) the plasma membrane. We have raised antibodies in rabbits against two synthetic peptides representing the hydrophilic N- or C-terminal region of PrlA. Using these antibodies as probes in cell fractionation experiments, we confirm that PrlA is an integral membrane protein of the plasma membrane of E. coli. Fab fragments prepared from each of the two antisera specifically inhibit protein export by interfering with the binding of preproteins to the plasma membrane. Inhibition of preprotein binding and export by Fab fragments was shown in a cell-free translocation system: precursors for LamB (an integral membrane protein) and for a truncated form of MalE (a periplasmic protein) were first synthesized in a membrane-depleted E. coli-derived cell-free translocation system followed by posttranslational incubation with inverted vesicles derived from the plasma membrane of E. coli. Our data thus indicate that the N and C termini of PrlA are exposed to the cytoplasm. We discuss the possibility that the transmembrane segments of PrlA could be arranged in the lipid bilayer in a cylindrical fashion, thereby delimiting a protein conducting channel, with a signal sequence binding domain represented, at least in part, by the N and C termini of PrlA. Such a channel would also contain a "stop-transfer" sequence binding domain that in response to a stop-transfer sequence would open the cylindrical channel to the lipid bilayer and permit displacement of the polypeptide from the channel to the lipid bilayer, resulting in membrane integration.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Watanabe M,Blobel G

doi

10.1073/pnas.86.6.1895

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

1895-9

issue

eissn

0027-8424

issn

1091-6490

journal_volume

pub_type

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Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.