The primary structure of high density apolipoprotein-glutamine-I.

abstract：:The C terminus of transcription factor NusA from Escherichia coli comprises two repeat units, which bind during antitermination to protein N from phage lambda. To delineate the structural basis of the NusA-lambdaN interaction, we attempted to crystallize the NusA C-terminal repeats in complex with a lambdaN peptide (r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonin I,Mühlberger R,Bourenkov GP,Huber R,Bacher A,Richter G,Wahl MC

更新日期：2004-09-21 00:00:00

abstract：:Synaptically released glutamate has been identified as a signal coupling excitatory neuronal activity to increased glucose utilization. The proposed mechanism of this coupling involves glutamate uptake into astrocytes resulting in increased intracellular Na+ (Nai+) and activation of the Na+/K+-ATPase. Increased metabo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatton JY,Pellerin L,Magistretti PJ

更新日期：2003-10-14 00:00:00

abstract：:Genetic manipulation of the alpha(2A)-adrenergic receptor (alpha(2A)-AR) in mice has revealed the role of this subtype in numerous responses, including agonist-induced hypotension and sedation. Unexpectedly, alpha(2)-agonist treatment of mice heterozygous for the alpha(2A)-AR (alpha(2A)-AR(+/-)) lowers blood pressure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tan CM,Wilson MH,MacMillan LB,Kobilka BK,Limbird LE

更新日期：2002-09-17 00:00:00

abstract：:Methane can be converted to triose dihydroxyacetone (DHA) by chemical processes with formaldehyde as an intermediate. Carbon dioxide, a by-product of various industries including ethanol/butanol biorefineries, can also be converted to formaldehyde and then to DHA. DHA, upon entry into a cell and phosphorylation to DHA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Chauliac D,Rhee MS,Panneerselvam A,Ingram LO,Shanmugam KT

更新日期：2018-04-24 00:00:00

abstract：:The human spliceosome is a large ribonucleoprotein complex that catalyzes pre-mRNA splicing. It consists of five snRNAs and more than 200 proteins. Because of this complexity, much work has focused on the Saccharomyces cerevisiae spliceosome, viewed as a highly simplified system with fewer than half as many splicing f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stark MR,Dunn EA,Dunn WS,Grisdale CJ,Daniele AR,Halstead MR,Fast NM,Rader SD

更新日期：2015-03-17 00:00:00

abstract：:Newly emerging plants provide the best forage for herbivores. To exploit this fleeting resource, migrating herbivores align their movements to surf the wave of spring green-up. With new technology to track migrating animals, the Green Wave Hypothesis has steadily gained empirical support across a diversity of migrator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geremia C,Merkle JA,Eacker DR,Wallen RL,White PJ,Hebblewhite M,Kauffman MJ

更新日期：2019-12-17 00:00:00

abstract：:The homogeneous exchange process whereby IgG molecules adsorbed onto latex particles are replaced by IgG molecules from the bulk solution was studied by means of 125I radiolabeling. The exchange mechanism was investigated on surfaces saturated with either labeled or unlabeled proteins in the presence of a solution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ball V,Huetz P,Elaissari A,Cazenave JP,Voegel JC,Schaaf P

更新日期：1994-07-19 00:00:00

abstract：:We report the successful infection of two rabbit T-cell lines and one rabbit macrophage line with human immunodeficiency virus 1 (HIV-1). One T-cell line was a herpesvirus ateles transformant, the other T-cell line was a human T-cell leukemia virus I transformant, and the macrophage line was a simian virus 40 transfor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kulaga H,Folks TM,Rutledge R,Kindt TJ

更新日期：1988-06-01 00:00:00

abstract：:There are two competing hypotheses for the origin of megaliths in Europe. The conventional view from the late 19th and early 20th centuries was of a single-source diffusion of megaliths in Europe from the Near East through the Mediterranean and along the Atlantic coast. Following early radiocarbon dating in the 1970s,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz Paulsson B

更新日期：2019-02-26 00:00:00

abstract：:Although the role of APP and PSEN genes in genetic Alzheimer's disease (AD) cases is well established, fairly little is known about the molecular mechanisms affecting Abeta generation in sporadic AD. Deficiency in Abeta clearance is certainly a possibility, but increased expression of proteins like APP or BACE1/beta-s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hébert SS,Horré K,Nicolaï L,Papadopoulou AS,Mandemakers W,Silahtaroglu AN,Kauppinen S,Delacourte A,De Strooper B

更新日期：2008-04-29 00:00:00

abstract：:Laser-induced fluorescence at 337-nm excitation was used in vivo to differentiate neoplastic [cervical intraepithelial neoplasia (CIN)], nonneoplastic abnormal (inflammation and human papilloma viral infection), and normal cervical tissues. A colposcope (low-magnification microscope used to view the cervix with reflec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramanujam N,Mitchell MF,Mahadevan A,Warren S,Thomsen S,Silva E,Richards-Kortum R

更新日期：1994-10-11 00:00:00

abstract：:30S ribosomal proteins from Bacillus stearothermophilus (B. proteins) have been fractionated and characterized with respect to their ability to replace various E. coli 30S proteins (E. proteins) in the E. coli 30S ribosome reconstitution system. The functional counterparts of all the E. proteins, except S1, S6, S9, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higo K,Held W,Kahan L,Nomura M

更新日期：1973-03-01 00:00:00

abstract：:Cultured fibroblast cells derived from a skin biopsy sample taken from normal human adult were exposed to a potent carcinogen, 4-nitroquinoline 1-oxide. Alterations of cell growth pattern such as higher density and piling up of cells were noticed in some fractions of cultures that were successively subcultured after n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kakunaga T

更新日期：1978-03-01 00:00:00

abstract：:Vasopressin's action in renal cells to regulate water transport depends on protein phosphorylation. Here we used mass spectrometry-based quantitative phosphoproteomics to identify signaling pathways involved in the short-term V2-receptor-mediated response in cultured collecting duct cells (mpkCCD) from mouse. Using St...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rinschen MM,Yu MJ,Wang G,Boja ES,Hoffert JD,Pisitkun T,Knepper MA

更新日期：2010-02-23 00:00:00

abstract：:The human pathogen Mycobacterium tuberculosis encodes a proteasome that carries out regulated degradation of bacterial proteins. It has been proposed that the proteasome contributes to nitrogen metabolism in M. tuberculosis, although this hypothesis had not been tested. Upon assessing M. tuberculosis growth in several...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker SH,Jastrab JB,Dhabaria A,Chaton CT,Rush JS,Korotkov KV,Ueberheide B,Darwin KH

更新日期：2019-02-19 00:00:00

abstract：:Atherosclerosis is associated with alterations in nitric oxide (NO)/cGMP signaling. In early stages of the disease, inflammatory and possibly other cells produce reactive oxygen species that scavenge vasoprotective NO. In addition to the oxidative stress, expression and activity of enzymes downstream to NO formation m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melichar VO,Behr-Roussel D,Zabel U,Uttenthal LO,Rodrigo J,Rupin A,Verbeuren TJ,Kumar H S A,Schmidt HH

更新日期：2004-11-23 00:00:00

abstract：:Numerous relatively short regions within intrinsically disordered proteins (IDPs) serve as molecular recognition elements (MoREs). They fold into ordered structures upon binding to their partner molecules. Currently, there is still a lack of in-depth understanding of how coupled binding and folding occurs in MoREs. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Chu X,Longhi S,Roche P,Han W,Wang E,Wang J

更新日期：2013-10-01 00:00:00

abstract：:In most Gram-positive bacteria, serine-46-phosphorylated HPr (P-Ser-HPr) controls the expression of numerous catabolic genes ( approximately 10% of their genome) by acting as catabolite corepressor. HPr kinase/phosphorylase (HprK/P), the bifunctional sensor enzyme for catabolite repression, phosphorylates HPr, a phosp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mijakovic I,Poncet S,Galinier A,Monedero V,Fieulaine S,Janin J,Nessler S,Marquez JA,Scheffzek K,Hasenbein S,Hengstenberg W,Deutscher J

更新日期：2002-10-15 00:00:00

abstract：:Plants constantly undergo external mechanical loads such as wind or touch and respond to these stimuli by acclimating their growth processes. A fascinating feature of this mechanical-induced growth response is that it can occur rapidly and at long distance from the initial site of stimulation, suggesting the existence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Louf JF,Guéna G,Badel E,Forterre Y

更新日期：2017-10-17 00:00:00

abstract：:We compared students' self-reported perception of learning with their actual learning under controlled conditions in large-enrollment introductory college physics courses taught using 1) active instruction (following best practices in the discipline) and 2) passive instruction (lectures by experienced and highly rated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deslauriers L,McCarty LS,Miller K,Callaghan K,Kestin G

更新日期：2019-09-24 00:00:00

abstract：:Transcriptional activation by steroid hormones is often associated with the appearance of a DNase I hypersensitive site resulting from a local alteration of the nucleoprotein structure of the promoter. For the mouse mammary tumor virus long terminal repeat, a viral promoter under glucocorticoid control, a model has be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carr KD,Richard-Foy H

更新日期：1990-12-01 00:00:00

abstract：:This paper reports a general in situ method to grow a polymer conjugate solely from the C terminus of a recombinant protein. GFP was fused at its C terminus with an intein; cleavage of the intein provided a unique thioester moiety at the C terminus of GFP that was used to install an atom transfer radical polymerizatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gao W,Liu W,Christensen T,Zalutsky MR,Chilkoti A

更新日期：2010-09-21 00:00:00

abstract：:We describe in this study punchless, a nonpathogenic mutant from the rice blast fungus M. grisea, obtained by plasmid-mediated insertional mutagenesis. As do most fungal plant pathogens, M. grisea differentiates an infection structure specialized for host penetration called the appressorium. We show that punchless dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clergeot PH,Gourgues M,Cots J,Laurans F,Latorse MP,Pepin R,Tharreau D,Notteghem JL,Lebrun MH

更新日期：2001-06-05 00:00:00

abstract：:An improved electron cloud bolometer is analyzed. In this device the cloud temperature is read out not via thermal noise induced by the electrons in a coupled LC circuit but via shift in their axial oscillation frequency in the Penning trap confining them. This shift occurs because as the electron cloud expands with i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dehmelt H

更新日期：1994-07-05 00:00:00

abstract：:We determined the frequency of integrated viral DNA in the livers of three woodchucks chronically infected with the woodchuck hepatitis virus before and during 30 weeks of therapy with the nucleoside analog L-FMAU [1-(2-fluoro-5-methyl-beta, L-arabinofuranosyl)uracil, clevudine]. We found that although viral covalentl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Summers J,Mason WS

更新日期：2004-01-13 00:00:00

abstract：:Transcription of the type 1 herpes simplex virus (HSV-1) genome in trigeminal ganglia of latently infected mice was studied using in situ hybridization. Probes representative of each temporal gene class were used to determine the regions of the genome that encode the transcripts present in latently infected cells. Pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deatly AM,Spivack JG,Lavi E,Fraser NW

更新日期：1987-05-01 00:00:00

abstract：:The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin conten...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Studer MH,DeMartini JD,Davis MF,Sykes RW,Davison B,Keller M,Tuskan GA,Wyman CE

更新日期：2011-04-12 00:00:00

abstract：:Excited electronic states created by UV excitation of the diribonucleoside monophosphates ApA, ApG, ApC, ApU, and CpG were studied by the femtosecond transient-absorption technique. Bleach recovery signals recorded at 252 nm show that long-lived excited states are formed in all five dinucleosides. The lifetimes of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takaya T,Su C,de La Harpe K,Crespo-Hernández CE,Kohler B

更新日期：2008-07-29 00:00:00

abstract：:Presynaptic and postsynaptic differentiation occurs at axodendritic contacts between CNS neurons. Synaptic adhesion mediated by synaptic cell adhesion molecule (SynCAM) and beta-neurexins/neuroligins triggers presynaptic differentiation. The signals that trigger postsynaptic differentiation are, however, unknown. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nam CI,Chen L

更新日期：2005-04-26 00:00:00

abstract：:The ability to analyze and isolate cells based on the expression of specific surface markers has increased our understanding of cell biology and produced numerous applications for biomedicine. However, established cell-sorting platforms rely on labels that are limited in number due to biophysical constraints, such as ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dahotre SN,Chang YM,Wieland A,Stammen SR,Kwong GA

更新日期：2018-04-24 00:00:00