MDC1 regulates intra-S-phase checkpoint by targeting NBS1 to DNA double-strand breaks.

Abstract:

:The product of the Nijmegen breakage syndrome gene (NBS1) plays crucial roles in DNA damage response through its association with many proteins, including MRE11 and RAD50. However, it remains to be determined exactly how NBS1 accumulates at or near DNA double-strand breaks. Here we report that MDC1 directly binds to NBS1 and targets NBS1 to the sites of DNA damage. The MDC1-NBS1 interaction occurs through a specific region (residues 200-420) of MDC1, which contains multiple consensus casein kinase 2 (CK2) phosphorylation sites. In addition, this interaction requires both the forkhead-associated (FHA) and tandem BRCA1 C-terminal (BRCT) domains of NBS1. Disruption of the MDC1-NBS1 interaction results in failure of NBS1 accumulation at DNA double-strand breaks and impairment of intra-S checkpoint activation. These studies provide important mechanistic insights as to how MDC1 regulates NBS1 and the intra-S-phase checkpoint in response to DNA damage.

authors

Wu L,Luo K,Lou Z,Chen J

doi

10.1073/pnas.0802885105

subject

Has Abstract

pub_date

2008-08-12 00:00:00

pages

11200-5

issue

32

eissn

0027-8424

issn

1091-6490

pii

0802885105

journal_volume

105

pub_type

杂志文章
  • Enzymatic repair of DNA, 1. Purification of two enzymes involved in the excision of thymine dimers from ultraviolet-irradiated DNA.

    abstract::Two nucleases that catalyze the excision of photoproducts from UV-irradiated DNA have been extensively purified from M. luteus (M. lysodeikticus). The first enzyme, an endonuclease, has been purified 5000-fold and is free of conflicting nuclease activities. It introduces single-strand breaks into irradiated DNA but do...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.63.1.144

    authors: Kaplan JC,Kushner SR,Grossman L

    更新日期:1969-05-01 00:00:00

  • Modification of microtubule steady-state dynamics by phosphorylation of the microtubule-associated proteins.

    abstract::Phosphorylation of purified microtubule-associated proteins (MAPs) inhibits the rate and extent of MAP-stimulated microtubule assembly. The extent of microtubule assembly is reduced as a result of a decrease in the fraction of tubulin polymerized, without a significant change in the critical protein concentration. The...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.6.3413

    authors: Jameson L,Caplow M

    更新日期:1981-06-01 00:00:00

  • A quantitative method for evaluating the stabilities of nucleic acids.

    abstract::We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of nucleic acid duplexes. We demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free energy differences between an initially f...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.11.6113

    authors: Gelfand CA,Plum GE,Mielewczyk S,Remeta DP,Breslauer KJ

    更新日期:1999-05-25 00:00:00

  • Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation.

    abstract::Certain types of human papillomaviruses (HPVs) are etiologically linked to cervical cancer. Their transforming capacity is encoded by a polycistronic premRNA, where alternative splicing leads to the translation of functional distinct proteins such as E6, E6*, and E7. Here we show that splicing of HPV16 E6/E7 ORF casse...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1002620107

    authors: Rosenberger S,De-Castro Arce J,Langbein L,Steenbergen RD,Rösl F

    更新日期:2010-04-13 00:00:00

  • Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii.

    abstract::State transition in photosynthesis is a short-term balancing mechanism of energy distribution between photosystem I (PSI) and photosystem II (PSII). When PSII is preferentially excited (state 2), a pool of mobile light-harvesting complex II (LHCII) antenna proteins is thought to migrate from PSII to PSI, but biochemic...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0509952103

    authors: Takahashi H,Iwai M,Takahashi Y,Minagawa J

    更新日期:2006-01-10 00:00:00

  • Prediction of an U2S3-type polymorph of Al2O3 at 3.7 Mbar.

    abstract::We predict by first principles a phase transition in alumina at approximately 3.7 Mbar and room temperature from the CaIrO(3)-type polymorph to another with the U(2)S(3)-type structure. Because alumina is used as window material in shock-wave experiments, this transformation should be important for the analysis of sho...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0711925105

    authors: Umemoto K,Wentzcovitch RM

    更新日期:2008-05-06 00:00:00

  • The anaphase promoting complex/cyclosome is required during development for modified cell cycles.

    abstract::Animals and plants use modified cell cycles to achieve particular developmental strategies. In one common example, most animals and plants have tissues in which the cells become polyploid or polytene by means of an S-G cycle, but the mechanism by which mitosis is inhibited in the endo cycle is not understood. The Dros...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.172391099

    authors: Kashevsky H,Wallace JA,Reed BH,Lai C,Hayashi-Hagihara A,Orr-Weaver TL

    更新日期:2002-08-20 00:00:00

  • Harm to others outweighs harm to self in moral decision making.

    abstract::Concern for the suffering of others is central to moral decision making. How humans evaluate others' suffering, relative to their own suffering, is unknown. We investigated this question by inviting subjects to trade off profits for themselves against pain experienced either by themselves or an anonymous other person....

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,随机对照试验

    doi:10.1073/pnas.1408988111

    authors: Crockett MJ,Kurth-Nelson Z,Siegel JZ,Dayan P,Dolan RJ

    更新日期:2014-12-02 00:00:00

  • Redox-dependent stability, protonation, and reactivity of cysteine-bound heme proteins.

    abstract::Cysteine-bound hemes are key components of many enzymes and biological sensors. Protonation (deprotonation) of the Cys ligand often accompanies redox transformations of these centers. To characterize these phenomena, we have engineered a series of Thr78Cys/Lys79Gly/Met80X mutants of yeast cytochrome c (cyt c) in which...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1317173111

    authors: Zhong F,Lisi GP,Collins DP,Dawson JH,Pletneva EV

    更新日期:2014-01-21 00:00:00

  • A unified model of protein dynamics.

    abstract::Protein functions require conformational motions. We show here that the dominant conformational motions are slaved by the hydration shell and the bulk solvent. The protein contributes the structure necessary for function. We formulate a model that is based on experiments, insights from the physics of glass-forming liq...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900336106

    authors: Frauenfelder H,Chen G,Berendzen J,Fenimore PW,Jansson H,McMahon BH,Stroe IR,Swenson J,Young RD

    更新日期:2009-03-31 00:00:00

  • Retroviral analysis of cardiac morphogenesis: discontinuous formation of coronary vessels.

    abstract::Cellular progenitors of the coronary vasculature are believed to enter the chicken heart during epicardial morphogenesis between stages 17 and 27 (days 3-5) of egg incubation. To trace cells which give rise to the coronary arteries in vivo, we applied retroviral cell tagging procedures and analyzed clonal populations ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.20.9504

    authors: Mikawa T,Fischman DA

    更新日期:1992-10-15 00:00:00

  • Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix.

    abstract::Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase involved in development and human disease, including cancer. It is currently thought that the four-point one, ezrin, radixin, moesin (FERM)-kinase domain linker, which contains autophosphorylation site tyrosine (Y) 397, is not required for in vivo FAK functi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1614894114

    authors: Heim JB,Squirewell EJ,Neu A,Zocher G,Sominidi-Damodaran S,Wyles SP,Nikolova E,Behrendt N,Saunte DM,Lock-Andersen J,Gaonkar KS,Yan H,Sarkaria JN,Krendel M,van Deursen J,Sprangers R,Stehle T,Böttcher RT,Lee JH,Ordog T

    更新日期:2017-04-11 00:00:00

  • In vivo bioluminescence imaging reveals copper deficiency in a murine model of nonalcoholic fatty liver disease.

    abstract::Copper is a required metal nutrient for life, but global or local alterations in its homeostasis are linked to diseases spanning genetic and metabolic disorders to cancer and neurodegeneration. Technologies that enable longitudinal in vivo monitoring of dynamic copper pools can help meet the need to study the complex ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1613628113

    authors: Heffern MC,Park HM,Au-Yeung HY,Van de Bittner GC,Ackerman CM,Stahl A,Chang CJ

    更新日期:2016-12-13 00:00:00

  • An uncorrelated relaxed-clock analysis suggests an earlier origin for flowering plants.

    abstract::We present molecular dating analyses for land plants that incorporate 33 fossil calibrations, permit rates of molecular evolution to be uncorrelated across the tree, and take into account uncertainties in phylogenetic relationships and the fossil record. We attached a prior probability to each fossil-based minimum age...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 历史文章,杂志文章

    doi:10.1073/pnas.1001225107

    authors: Smith SA,Beaulieu JM,Donoghue MJ

    更新日期:2010-03-30 00:00:00

  • UVRAG is required for virus entry through combinatorial interaction with the class C-Vps complex and SNAREs.

    abstract::Enveloped viruses exploit the endomembrane system to enter host cells. Through a cascade of membrane-trafficking events, virus-bearing vesicles fuse with acidic endosomes and/or lysosomes mediated by SNAREs triggering viral fusion. However, the molecular mechanisms underlying this process remain elusive. Here, we foun...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1320629111

    authors: Pirooz SD,He S,Zhang T,Zhang X,Zhao Z,Oh S,O'Connell D,Khalilzadeh P,Amini-Bavil-Olyaee S,Farzan M,Liang C

    更新日期:2014-02-18 00:00:00

  • Mitochondrial creatine kinase: a major constituent of pathological inclusions seen in mitochondrial myopathies.

    abstract::Overaccumulation of abnormally organized mitochondria in so-called "ragged-red" skeletal muscle fibers is a morphological hallmark of mitochondrial myopathies, in particular of mitochondrial encephalomyopathies. Characteristic for the abnormal mitochondria is the occurrence of highly ordered crystalline inclusions. Im...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.11.5089

    authors: Stadhouders AM,Jap PH,Winkler HP,Eppenberger HM,Wallimann T

    更新日期:1994-05-24 00:00:00

  • The inhibition of LSD1 via sequestration contributes to tau-mediated neurodegeneration.

    abstract::Tauopathies are a class of neurodegenerative diseases associated with pathological tau. Despite many advances in our understanding of these diseases, the direct mechanism through which tau contributes to neurodegeneration remains poorly understood. Previously, our laboratory implicated the histone demethylase LSD1 in ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2013552117

    authors: Engstrom AK,Walker AC,Moudgal RA,Myrick DA,Kyle SM,Bai Y,Rowley MJ,Katz DJ

    更新日期:2020-11-17 00:00:00

  • Separation of telomerase functions by reverse genetics.

    abstract::The canonical function of the human telomerase protein (hTERT) is to synthesize telomeric DNA, but it has other biological activities, including enhancing cell proliferation, decreasing apoptosis, regulating DNA damage responses, and increasing cellular proliferative lifespan. The mechanistic relationships among these...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1112414108

    authors: Mukherjee S,Firpo EJ,Wang Y,Roberts JM

    更新日期:2011-12-13 00:00:00

  • Selective viral vector transduction of ErbB4 expressing cortical interneurons in vivo with a viral receptor-ligand bridge protein.

    abstract::Both treatment of disease and basic studies of complex tissues can benefit from directing viral vector infection to specific cell types. We have used a unique cell targeting method to direct viral vector transduction to cerebral cortical neurons expressing the neuregulin (NRG) receptor ErbB4; both NRG and ErbB4 have b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1006233107

    authors: Choi J,Young JA,Callaway EM

    更新日期:2010-09-21 00:00:00

  • Fidelity of replication of phage phi X174 DNA by DNA polymerase III holoenzyme: spontaneous mutation by misincorporation.

    abstract::DNA from phi X174 is replicated in vitro with a fidelity similar to that found genetically. A mutation of TAG leads to TGG may be induced, however, by varying the concentrations of deoxynucleoside triphosphates, with a frequency proportional to [dGTP]2/[dATP]. This complex concentration dependence is consistent with t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.76.10.4946

    authors: Fersht AR

    更新日期:1979-10-01 00:00:00

  • Information-theoretic model comparison unifies saliency metrics.

    abstract::Learning the properties of an image associated with human gaze placement is important both for understanding how biological systems explore the environment and for computer vision applications. There is a large literature on quantitative eye movement models that seeks to predict fixations from images (sometimes termed...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1510393112

    authors: Kümmerer M,Wallis TS,Bethge M

    更新日期:2015-12-29 00:00:00

  • On-line visualization of dendritic release of acetylcholinesterase from mammalian substantia nigra neurons.

    abstract::This study presents, to our knowledge, the first on-line measurement of acetylcholinesterase (AcChoEase) release from brain tissue. It is now well established that a soluble form of the enzyme is released from central nervous system neurons, and it has been proposed on indirect grounds that such release may occur not ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.9.3047

    authors: Llinás RR,Greenfield SA

    更新日期:1987-05-01 00:00:00

  • GTF2IRD2 is located in the Williams-Beuren syndrome critical region 7q11.23 and encodes a protein with two TFII-I-like helix-loop-helix repeats.

    abstract::Williams-Beuren syndrome (also known as Williams syndrome) is caused by a deletion of a 1.55- to 1.84-megabase region from chromosome band 7q11.23. GTF2IRD1 and GTF2I, located within this critical region, encode proteins of the TFII-I family with multiple helix-loop-helix domains known as I repeats. In the present wor...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404150101

    authors: Makeyev AV,Erdenechimeg L,Mungunsukh O,Roth JJ,Enkhmandakh B,Ruddle FH,Bayarsaihan D

    更新日期:2004-07-27 00:00:00

  • Type II protein arginine methyltransferase 5 (PRMT5) is required for circadian period determination in Arabidopsis thaliana.

    abstract::Posttranslational modification is an important element in circadian clock function from cyanobacteria through plants and mammals. For example, a number of key clock components are phosphorylated and thereby marked for subsequent ubiquitination and degradation. Through forward genetic analysis we demonstrate that prote...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1011987107

    authors: Hong S,Song HR,Lutz K,Kerstetter RA,Michael TP,McClung CR

    更新日期:2010-12-07 00:00:00

  • Disregulation of leukosialin (CD43, Ly48, sialophorin) expression in the B-cell lineage of transgenic mice increases splenic B-cell number and survival.

    abstract::Leukosialin (also known as Ly48, CD43, and sialophorin) is a major cell surface sialoglycoprotein found on a variety of hematopoietically derived cells. The precise function of this molecule is poorly understood but it has been implicated in cell proliferation and intercellular adhesion. We developed a transgenic mous...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.2.626

    authors: Dragone LL,Barth RK,Sitar KL,Disbrow GL,Frelinger JG

    更新日期:1995-01-17 00:00:00

  • Long-term potentiation involves increases in the probability of neurotransmitter release.

    abstract::There is great interest in understanding the mechanisms of expression underlying long-term potentiation (LTP). They are agreed to involve an increase in synaptic efficacy, which is described by three multiplicative parameters: p, the probability of neurotransmitter release; n, the number of active release sites; and q...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.11.5888

    authors: Schulz PE

    更新日期:1997-05-27 00:00:00

  • Interleukin 1 induces expression of the human immunodeficiency virus alone and in synergy with interleukin 6 in chronically infected U1 cells: inhibition of inductive effects by the interleukin 1 receptor antagonist.

    abstract::In the present study we have observed that interleukin (IL) 1 alpha or IL-1 beta directly induced expression of human immunodeficiency virus (HIV) in the latently infected human promonocytic cell line U1. In addition, IL-1 synergized with IL-6, but not with tumor necrosis factor, in the upregulation of virus expressio...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.1.108

    authors: Poli G,Kinter AL,Fauci AS

    更新日期:1994-01-04 00:00:00

  • Heterodimerization of type II phytochromes in Arabidopsis.

    abstract::Coimmunoprecipitation of members of the phytochrome red/farred photoreceptor family from plant extracts has been used to analyze their heteromeric binding interactions. Phytochrome (phy)B or phyD apoproteins with six myc epitopes fused to their N termini are biologically active when expressed in Arabidopsis. Immunopre...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404286101

    authors: Sharrock RA,Clack T

    更新日期:2004-08-03 00:00:00

  • Characterization of anti-anti-idiotypic antibodies that bind antigen and an anti-idiotype.

    abstract::Two mouse monoclonal anti-anti-idiotopic antibodies (anti-anti-Id, Ab3), AF14 and AF52, were prepared by immunizing BALB/c mice with rabbit polyclonal anti-idiotypic antibodies (anti-Id, Ab2) raised against antibody D1.3 (Ab1) specific for the antigen hen egg lysozyme. AF14 and AF52 react with an "internal image" mono...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.16.8697

    authors: Goldbaum FA,Velikovsky CA,Dall'Acqua W,Fossati CA,Fields BA,Braden BC,Poljak RJ,Mariuzza RA

    更新日期:1997-08-05 00:00:00

  • Feedback regulation of proliferation vs. differentiation rates explains the dependence of CD4 T-cell expansion on precursor number.

    abstract::The mechanisms regulating clonal expansion and contraction of T cells in response to immunization remain to be identified. A recent study established that there was a log-linear relation between CD4 T-cell precursor number (PN) and factor of expansion (FE), with a slope of ∼-0.5 over a range of 3-30,000 precursors per...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1019706108

    authors: Bocharov G,Quiel J,Luzyanina T,Alon H,Chiglintsev E,Chereshnev V,Meier-Schellersheim M,Paul WE,Grossman Z

    更新日期:2011-02-22 00:00:00